scholarly journals Comparative Extraction of Chlorophylls in Selected Forest and Savanna Mosses Using Dimethylsulphoxide and Acetone

2016 ◽  
Vol 8 (3) ◽  
pp. 347-353
Author(s):  
Fisayo S. AROYEHUN ◽  
Amos M. MAKINDE ◽  
Musibau O. ISA
Keyword(s):  
Statistical Analysis ◽  
Chlorophyll A ◽  
Natural Populations ◽  
Chlorophyll B ◽  
Total Chlorophyll ◽  
Standard Methods ◽  
Derived Savanna ◽  
Chlorophyll Accumulation ◽  
Significant Difference

The present study compared the extraction of chlorophylls from selected forest mosses (Hyophila involuta and Thuidium gratum) and derived savanna moss (Archidium ohioense) using dimethylsulphoxide (DMSO) and 80% acetone. The mosses were collected from natural populations found in the Central Campus of the Obafemi Awolowo University, Ile-Ife, Nigeria. The chlorophyll extractions process followed standard methods and the absorbance of the extracts were read on spectrophotometer at wavelengths of 645 nm and 663 nm respectively. The data obtained were later subjected to appropriate statistical analysis. The results showed that DMSO was a better chlorophyll extractant for mosses than 80% acetone. Although there were significant differences in the chlorophyll a, chlorophyll a/b ratio and total chlorophyll accumulation within all three species using DMSO and 80% acetone as extractant (P < 0.05), there was no significant difference in the chlorophyll b accumulation of all the three species (P > 0.05).

scholarly journals Effect Different Concentrations of Crude Oil on the Pigment Content and Protein Content of Hydrilla Verticillata Plant

2019 ◽  
pp. 2141-2148
Author(s):  
Sadiq Kadhum Lafta Alzurfi ◽  
Kasim Kadhim Alasedi ◽  
Noor Imad Abdulraheem
Keyword(s):  
Statistical Analysis ◽  
Crude Oil ◽  
Protein Content ◽  
Chlorophyll A ◽  
Hydrilla Verticillata ◽  
Gradual Decrease ◽  
Control Treatment ◽  
Chlorophyll B ◽  
Total Chlorophyll ◽  
Probability Level

The current study was conducted to determine the effect of crude oil on the pigments and protein content of Hydrilla verticillata plant from December 2018 to February 2019. Hydrilla verticillata was exposed to different concentrations of treatments (0.01,0.05,0.10,0.20) % of crude oil for 24 days. The pigments content was evaluated as total chlorophyll, a, b, carotene and protein content within plant tissues during the days (1, 8, 16 and 24).      The results of this study showed an increment in the total chlorophyll values of plant, which is exposed to different concentrations of crude oil compared to the control treatment during the first day of the experiment and decreased compared to control treatment during 8,16 and 24 days of the experiment. The recorded maximum value of total chlorophyll was (29.49) μg / g at 0.10% treatment during the first day and the statistical analysis showed significant differences below the probability level (P> 0.05) between the concentrations and the days of the experiment. Chlorophyll a, b and carotene of plant exhibit the overall trend of total chlorophyll. The values of chlorophyll b were higher than the values of chlorophyll and carotene.      The current study showed a gradual decrease in the total protein values of plants in crude oil during experiential period compared to control treatment, recording the highest values at the 0.01% during the first day (29.4 mg/g) and the lowest in the concentration of 0.05% during the day 24 was (3.5) mg / g, and the statistical analysis showed significant differences below the probability level (P> 0.05) between the days and concentrations of the experiment. The study concluded that a gradual decrease of total chlorophyll values during period experimental, recorded the chlorophyll b values higher compare to chlorophyll a and carotene. the gradual decrease of protein values with the increasing the concentration.

scholarly journals Seasonal Variation of Chlorophyll and Carotenoids in Leaves of Hancornia speciosa in Three Central Areas of the Cerrado of the State of Tocantins, Brazil

2018 ◽  
Vol 10 (3) ◽  
pp. 344
Author(s):  
Rafael J. de Oliveira ◽  
José Expedito C. da Silva ◽  
Davi B. das Chagas
Keyword(s):  
Seasonal Variation ◽  
Chlorophyll A ◽  
Temporal Analysis ◽  
Chlorophyll B ◽  
Total Chlorophyll ◽  
Dry Period ◽  
Rainy Period ◽  
Significant Difference ◽  
Spatio Temporal ◽  
Hancornia Speciosa

This study aimed to evaluate the seasonal variation in concentrations of chlorophyll a, b, total chlorophyll and carotenoids in leaves of Hancornia speciosa, Gomes, during the periods of the year, relating them to the main phenological events, periods (rainy, dry and transitions) and populations evaluated. The survey was performed in three sites and the spatio-temporal analysis divided into four periods (rainy, rainy-drytransition, dry and dry-rainy transition), with 10 replicates (matrix plants). The data were collected in average intervals of 33 days from October 2014 to April 2017. The extraction and calculation of the chlorophyll and carotenoid contents of the leaves were expressed in mg/g DM, according to the equations of Arnon (1949) and Lichtenthaler (1987). There was a significant difference between the periods and sites analyzed for all pigments and their relationships. There was a greater amount of chlorophyll a than chlorophyll b; this difference was greater in the dry period. In the rainy period, we found a greater amount of total chlorophyll, carotenoids and total chlorophyll/carotenoid ratio. The behavior for the species follows that already observed for deciduous plants, closely related to water availability.

scholarly journals Pemanfaatan Pupuk Organik Kulit Pisang Dan Azolla Terhadap Pertumbuhan Kangkung Darat Dengan Sistem Akuaponik

2019 ◽  
Vol 12 (01) ◽  
pp. 1-10
Author(s):  
Maria Sastriana ◽  
Ngadiani Ngadiani
Keyword(s):  
Chlorophyll Content ◽  
Chlorophyll A ◽  
Organic Fertilizer ◽  
Control Treatment ◽  
Banana Peel ◽  
Chlorophyll B ◽  
Total Chlorophyll ◽  
Azolla Pinnata ◽  
Randomized Design ◽  
Weight Test

The aim of this study was to compare the organic fertilizer of azolla pinnata and banana peel and a combination of azolla pinnata and banana peel with the same concentration of 800 ppm to the weight of harvest and the chlorophyll content of ground spinach (Ipomoea reptans Poir.). The experiment was carried out experimentally using a completely randomized design with control treatment, 800 ppm azolla pinnata, 800 ppm banana skin and a combination of azolla pinnata and banana peels 800 ppm which were repeated 6 times each. The application was carried out by pouring 800 ppm of azolla pinnata, 800 ppm of banana peel and a combination of azolla pinnata and 800 ppm banana peel on each gutter. The results showed that azolla pinnata and banana peel nutrition had a significant effect (P <0.05) on harvest weight and chlorophyll content of ground spinach plants (Ipomoea reptans Poir.). Nutrition azolla pinnata and banana peel with a concentration of 800 ppm can increase the weight of the land spinach (Ipomoea reptans Poir.) Which has the highest average. In testing the chlorophyll content, the results of chlorophyll a content, chlorophyll b content and the highest total chlorophyll content was found in azolla pinnata 800 ppm nutrition. The results of this study concluded that 800 ppm azolla pinnata, 800 ppm banana peel and 800 ppm combination of azolla pinnata and banana peel in the growth of ground spinach plants (Ipomoea reptans Poir.) Were effective to increase harvest weight and increase chlorophyll content of land spinach (Ipomoea reptans Poir.) Namely chlorophyll a, chlorophyll b and total chlorophyll of land spinach plants (Ipomoea reptans Poir.).   Keywords: ground kale, azolla pinnata, banana peel, harvest weight, test of chlorophyll content, chlorophyll a, chlorophyll b, total chlorophyll.

Effect of Nitrate and Ammonium Ions on the Pigment Content (Xanthophylls, Carotenes and Chlorophylls) of Ramalina Capitata

1995 ◽  
Vol 27 (2) ◽  
pp. 155-160
Author(s):  
M. A. Sanchez-Hoyos ◽  
E. Manrique
Keyword(s):  
Water Supply ◽  
Chlorophyll A ◽  
Nitrate Solution ◽  
Deionized Water ◽  
Pigment Content ◽  
Chlorophyll B ◽  
Ammonium Ions ◽  
Controlled Conditions ◽  
Significant Difference ◽  
Β Carotene

AbstractThalli of Ramalina capitata were sprayed with deionized water, 10 mM solutions of KNO3 or NH4Cl, or maintained without any water supply, for 10 days under controlled conditions. The lichens without a water supply showed an increase n antheraxanthin, lutein and β-carotene. The samples treated with deionized water showed a significant increase in violaxathin, antheraxanthin and β-carotene. The lack of a significant difference in zeaxanthin could indicate the absence of de-epoxidation of violaxanthin to zeaxanthin via antheraxanthin. Those samples treated with nitrate solution showed a significant increase in all pigments except for antheraxanthin. In contrast, ammonium treatment did not induce changes compared to water alone, except that antheraxanthin decreased. The lichens treated with nitrate exhibited a significant increase in both chlorophyll a and chlorophyll b, but ammonium affected only chlorophyll b.

scholarly journals Effect of nicosulfuron on chlorophyll content of marandu-grass cultivated in crop-forest-livestock integration systems

2020 ◽  
pp. 1876-1882
Author(s):  
Evander Alves Ferreira ◽  
Marcia Vitória Santos ◽  
Leandro Diego da Silva ◽  
Priscila Júnia Rodrigues da Cruz ◽  
Raul Ribeiro Silveira ◽  
...  
Keyword(s):  
Growth Rate ◽  
Chlorophyll Content ◽  
Chlorophyll A ◽  
Dry Matter ◽  
Agroforestry Systems ◽  
Chlorophyll B ◽  
Total Chlorophyll ◽  
Integration System ◽  
Total Chlorophyll Content ◽  
Urochloa Brizantha

The aim of this work was to study the effect of sub-doses of herbicide nicosulfuron on the levels of chlorophyll and growth of marandu-grass in an forest-livestock integration system. The treatments consisted of eucalyptus consortium with sorghum (BRS 655 hybrid) and forage Urochloa brizantha cv. Marandu (marandu-grass) at 12×2 and 12×3 m spacings in agroforestry systems, in addition to sorghum + marandu-grass and marandu-grass in monoculture. The doses of nicosulfuron applied at the marandu-grass were 0, 15 and 30 g ha-1. At 30 and 120 days after the application of nicosulfuron, chlorophyll a and chlorophyll b were measured usinf a portable chlorophyll meter, with six measurements per plant. Afterwards, the total chlorophyll content and chlorophyll a/b were calculated. We also measured the mass of the total dry matter of marandu-grass and growth rate of the culture. Marandu-grass plants cultivated in monoculture showed higher quantities of chlorophyll and the highest growth rates. The levels of chlorophyll a, b and chlorophyll a/b and the growth rate of marandu grass were similar between systems integrated with eucalyptus (12x2 and 12x3 m). The increase of herbicide dose negatively influenced on chlorophyll content in marandu-grass in all treatments evaluated. The use of nicosulfuron in doses of 25 and 50 g L-1 reduced the growth rate of marandu grass only when grown in monoculture

scholarly journals Anatomical and physiological aspects of cucumber graft

2018 ◽  
Vol 9 (2) ◽  
pp. 282-286
Author(s):  
Daniel Baron ◽  
Gustavo Francisco Rosalin Saraiva ◽  
Talita Silveira Amador ◽  
João Domingos Rodrigues ◽  
Rumy Goto ◽  
...  
Keyword(s):  
Experimental Design ◽  
Chlorophyll A ◽  
Chlorophyll B ◽  
Cucurbita Moschata ◽  
Total Chlorophyll ◽  
Cucumis Sativus L ◽  
Tukey Test ◽  
Grafting Technique ◽  
Grafting Through ◽  
Chlorophyll Index

The grafting of two cucumber scions (Cucumis sativus L.)—Taiko hybrid and Tsuyoi hybrid—onto a Tropical hybrid pumpkin rootstock (Cucurbita moschata L.) was studied. The study was conducted in a greenhouse. Plants grafted using the tongue approach grafting technique were compared with ungrafted plants. We evaluated the initial establishment of grafting through anatomical cuts on the region of union graft and examining leaves’ chlorophyll index (SPAD) and their levels of chlorophyll a, chlorophyll b and total chlorophyll. The experimental design utilized randomized blocks and the data were subjected to variance analysis (ANOVA) and the Tukey Test at 5% probability. The results indicate that Taiko cucumber scions are best grafted onto Tropical pumpkin rootstock, since these exhibits early grafting establishment. Moreover, when compared to grafting using Tsuyoi cucumber scions, Taiko cucumber scions present higher SPAD index values and levels of chlorophyll a and total chlorophyll. 

scholarly journals EVALUATION OF SALINITY STRESS EFFECTS ON CHANGES IN PHOTOSYNTHETIC PIGMENTS, HYDROGEN PEROXIDE AND OSMOLYTES IN SENSITIVE AND TOLERANT CULTIVARS OF WHEAT CROP

2020 ◽  
Vol 21 (supplement 1) ◽  
Author(s):  
Rasoul Khodavirdivand Keshtiban ◽  
Hassan Soltanloo ◽  
Seyedeh Sanaz Ramazanpour ◽  
Vahid Shariati ◽  
Vahid Shariati
Keyword(s):  
Hydrogen Peroxide ◽  
Chlorophyll A ◽  
Salinity Stress ◽  
Chinese Spring ◽  
Sampling Time ◽  
Wheat Crop ◽  
Chlorophyll B ◽  
Total Chlorophyll ◽  
Wheat Cultivars ◽  
Total Carbohydrates

Investigation of wheat response to salinity stress can help to better understand the effective defense mechanisms of salinity stress tolerance. For this purpose, biochemical and physiological traits related to salinity tolerance in wheat cultivars were evaluated at Gorgan University of Agricultural Sciences and Natural Resources in 2019. Experimental factors, included two wheat crop cultivars (Sarc and Chinese spring as tolerant and susceptible wheat cultivars, respectively) and sampling time series (zero or control, 24, 48, 72, and 96 h) were examined in a factorial experiment based on a completely randomized design with three replications. Salinity stress was applied with sodium chloride at a concentration of 250 mM to uniform 10-day-old seedlings at the two-leaf stage, followed by sampling of shoot tissue. The studied traits were hydrogen peroxide (H2O2 ), chlorophyll a, chlorophyll b, total chlorophyll, chlorophyllase, carotenoids, proline, and total carbohydrates. Results of analysis of variance (ANOVA) indicated significant effects of genotype, time, and interaction of genotype × time (except H2O2 and total carbohydrates) on all the studied traits. Results of interaction of genotype × time showed although the trend of changes in the studied traits, depending on the type of cultivar and the sampling time were different, but generally, the susceptible Chinese spring cultivar contained higher levels of chlorophyllase and carotenoids than the control time at the end of sampling time and also higher H2O2 levels than the Sarc tolerant cultivar, while the Sarc tolerant cultivar, on the other hand, contained higher levels of chlorophyll a, chlorophyll b, total chlorophyll, and proline than the control time at the end of sampling time and also greater total carbohydrates than the susceptible Chinese spring cultivar. The results confirm the higher capacity of the antioxidant defense system of Sarc tolerant cultivar than the susceptible Chinese spring cultivar. Therefore

scholarly journals Antioxidant properties of pepper (Capsicum annuum L.) depending on its cultivar and fruit colouration  

2021 ◽  
Author(s):  
Agnieszka Żurawik ◽  
Dorota Jadczak ◽  
Nikolay Panayotov ◽  
Piotr Żurawik
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Chlorophyll A ◽  
Antioxidant Properties ◽  
Temperate Climate ◽  
Chlorophyll B ◽  
Total Chlorophyll ◽  
Climate Conditions ◽  
Biological Value ◽  
Fruit Colouration

Due to its nutritional, health-promoting and taste-related values, new cultivars are introduced every year. The aim of the study was to assess the biological value of Polish and Bulgarian cultivars of pepper grown in moderate climate conditions and collected at different degrees of maturity. Ascorbic acid, carotenoids, polyphenols, chlorophyll a, chlorophyll b, total chlorophyll and antioxidant activity (DPPH) were determined in air-dried fruit. The investigation included five Polish cultivars: Roberta, Marta Polka, Etiuda, Trapez, and Cyklon and five Bulgarian cultivars: Bulgarski Ratung, Sivriya, Kurtovska Kapiya, Delikates, and Dzuliunska Shipka. Its design involved randomised sub-blocks, with three replications comprising nine plants each. The area of a single plot was 1.44 m<sup>2 </sup>(1.2 m × 1.2 m). The study confirmed the strong antioxidant properties of pepper grown in the field, without cover, and under temperate climate conditions. The vegetable is rich in vitamin C, polyphenols, carotenoids, chlorophyll pigments, and shows high antioxidant activity. However, the biological value of pepper is cultivar-dependent and is also determined by the fruit colouration degree. The coloured fruits are richer in vitamin C and carotenoids than the green ones, and when matured, they have greater antioxidant capacity. Green fruits contain more chlorophyll a, chlorophyll b and total chlorophyll than the coloured ones. The fruits of cv. Etiuda were the richest in vitamin C, of cv. Sivriya in polyphenols, of cv. Dzuliunska Shipka in carotenoids, and of cv. Trapez in total chlorophyll. Cv. Sivriya showed the strongest antioxidant properties.  

Acifluorfen Tolerance inLycopersicon

Weed Science ◽  
1992 ◽  
Vol 40 (3) ◽  
pp. 413-417 ◽  
Author(s):  
Jacqueline A. Ricotta ◽  
John B. Masiunas
Keyword(s):  
Free Radical ◽  
Chlorophyll A ◽  
Negative Correlation ◽  
Surface Characteristic ◽  
Chlorophyll B ◽  
Total Chlorophyll

Studies were conducted to determine the mechanism of acifluorfen tolerance within theLycopersicongenus. Absorption of14C-acifluorfen was not correlated with tolerance. There was a negative correlation (r = −0.57) between absorption 24 h after treatment and wax density. No other surface characteristic correlated with absorption. Less than 3% of absorbed14C was translocated and there was no metabolism of acifluorfen. All genotypes were susceptible to paraquat, and acifluorfen-tolerant genotypes had lower levels of ascorbate than susceptible genotypes, implying that free radical protectant systems were not involved in tolerance. Genotypes varied in amounts of chlorophyll a, chlorophyll b, and total chlorophyll but the differences did not correlate to acifluorfen tolerance.

scholarly journals Synthesis, Characterization and Herbicidal Activity of Amide Derivatives of Glyphosate

2018 ◽  
Vol 34 (5) ◽  
pp. 2378-2383 ◽  
Author(s):  
Jatinder Pal Kaur Gill ◽  
Nidhi Sethi ◽  
Anand Mohan
Keyword(s):  
Chlorophyll Content ◽  
Chlorophyll A ◽  
Structure Analysis ◽  
Herbicidal Activity ◽  
Chlorophyll B ◽  
Total Chlorophyll ◽  
Total Chlorophyll Content ◽  
Amide Derivatives ◽  
The Common ◽  
Derivatives Of

The present work includes synthesis of a series of amide derivatives of glyphosate and their characterization. The structure analysis of these new derivatives was done with the help of FTIR and 1H NMR, Further, their herbicidal activity was analyzed on one of the common weeds (Parthenium hysterophorus). Under the influence of amide derivatives of glyphosate it was found that the chlorophyll content (Chlorophyll a, Chlorophyll b and total Chlorophyll content) of the weed was found to lessen than the control. Moreover, these synthesized derivatives are less polar as compared to the parent glyphosate molecule thereby can emphatically reduce the problem of their leaching into the groundwater.

Sign in / Sign up

Export Citation Format

Share Document