Anatomical and physiological aspects of cucumber graft

The grafting of two cucumber scions (Cucumis sativus L.)—Taiko hybrid and Tsuyoi hybrid—onto a Tropical hybrid pumpkin rootstock (Cucurbita moschata L.) was studied. The study was conducted in a greenhouse. Plants grafted using the tongue approach grafting technique were compared with ungrafted plants. We evaluated the initial establishment of grafting through anatomical cuts on the region of union graft and examining leaves’ chlorophyll index (SPAD) and their levels of chlorophyll a, chlorophyll b and total chlorophyll. The experimental design utilized randomized blocks and the data were subjected to variance analysis (ANOVA) and the Tukey Test at 5% probability. The results indicate that Taiko cucumber scions are best grafted onto Tropical pumpkin rootstock, since these exhibits early grafting establishment. Moreover, when compared to grafting using Tsuyoi cucumber scions, Taiko cucumber scions present higher SPAD index values and levels of chlorophyll a and total chlorophyll.

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The effect of drought stress and nitrogen fertilization on the active ingredient, chlorophyll A and B, and chlorophyll index of henna

Nexo Revista Científica ◽

10.5377/nexo.v33i02.10802 ◽

2020 ◽

Vol 33 (02) ◽

pp. 694-706

Author(s):

Hassan Sarhadi ◽

Jahanfar Daneshian ◽

Seyyed Alireza Valadabadi ◽

Hossein Heidary Sharafabad ◽

...

Keyword(s):

Drought Stress ◽

Chlorophyll A ◽

Active Ingredient ◽

Block Design ◽

N Fertilization ◽

Water Requirement ◽

Plant Water ◽

Chlorophyll B ◽

Chlorophyll A And B ◽

Chlorophyll Index

The response of active ingredient, chlorophyll a and b contents, and chlorophyll index of henna to different rates of N fertilization and drought stress was explored in a split-split-plot experiment based on a randomized complete block design with four replications in the research farm of Jiroft Branch, Islamic Azad University, Jiroft, Iran in the 2016-2017 growing season. In the current study, the main plot was assigned to drought stress at three levels (including irrigation to supply 100%, 75% or 50% of plant water requirement), the sub-plot was assigned to N fertilization at three rates (50, 100 or 150 kg ha-1 pure N), and the sub-sub-plot was assigned to ecotype at three levels (Bami, Bushehri, and Rudbari). The results showed that the effect of drought level was significant on active ingredient and chlorophyll index at the p < 0.01 level and on chlorophyll a, total chlorophyll, and chlorophyll a/b ratio at the p < 0.05 level. But, it could not influence chlorophyll b significantly. The effect of N fertilization was significant on most studied traits at the p< 0.01 level, but its effect was significant on the chlorophyll index at the p < 0.05 level and insignificant on dye and chlorophyll b. According to the results, it seems that the application of 100 kg ha-1 N and the irrigation to supply 75% of plant water requirement can contribute to having more fresh plants with the higher active ingredient and dye contents.

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Pemanfaatan Pupuk Organik Kulit Pisang Dan Azolla Terhadap Pertumbuhan Kangkung Darat Dengan Sistem Akuaponik

STIGMA: Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa ◽

10.36456/stigma.vol12.no01.a1854 ◽

2019 ◽

Vol 12 (01) ◽

pp. 1-10

Author(s):

Maria Sastriana ◽

Ngadiani Ngadiani

Keyword(s):

Chlorophyll Content ◽

Chlorophyll A ◽

Organic Fertilizer ◽

Control Treatment ◽

Banana Peel ◽

Chlorophyll B ◽

Total Chlorophyll ◽

Azolla Pinnata ◽

Randomized Design ◽

Weight Test

The aim of this study was to compare the organic fertilizer of azolla pinnata and banana peel and a combination of azolla pinnata and banana peel with the same concentration of 800 ppm to the weight of harvest and the chlorophyll content of ground spinach (Ipomoea reptans Poir.). The experiment was carried out experimentally using a completely randomized design with control treatment, 800 ppm azolla pinnata, 800 ppm banana skin and a combination of azolla pinnata and banana peels 800 ppm which were repeated 6 times each. The application was carried out by pouring 800 ppm of azolla pinnata, 800 ppm of banana peel and a combination of azolla pinnata and 800 ppm banana peel on each gutter. The results showed that azolla pinnata and banana peel nutrition had a significant effect (P <0.05) on harvest weight and chlorophyll content of ground spinach plants (Ipomoea reptans Poir.). Nutrition azolla pinnata and banana peel with a concentration of 800 ppm can increase the weight of the land spinach (Ipomoea reptans Poir.) Which has the highest average. In testing the chlorophyll content, the results of chlorophyll a content, chlorophyll b content and the highest total chlorophyll content was found in azolla pinnata 800 ppm nutrition. The results of this study concluded that 800 ppm azolla pinnata, 800 ppm banana peel and 800 ppm combination of azolla pinnata and banana peel in the growth of ground spinach plants (Ipomoea reptans Poir.) Were effective to increase harvest weight and increase chlorophyll content of land spinach (Ipomoea reptans Poir.) Namely chlorophyll a, chlorophyll b and total chlorophyll of land spinach plants (Ipomoea reptans Poir.). Keywords: ground kale, azolla pinnata, banana peel, harvest weight, test of chlorophyll content, chlorophyll a, chlorophyll b, total chlorophyll.

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Effect of nicosulfuron on chlorophyll content of marandu-grass cultivated in crop-forest-livestock integration systems

Australian Journal of Crop Science ◽

10.21475/ajcs.20.14.12.2631 ◽

2020 ◽

pp. 1876-1882

Author(s):

Evander Alves Ferreira ◽

Marcia Vitória Santos ◽

Leandro Diego da Silva ◽

Priscila Júnia Rodrigues da Cruz ◽

Raul Ribeiro Silveira ◽

...

Keyword(s):

Growth Rate ◽

Chlorophyll Content ◽

Chlorophyll A ◽

Dry Matter ◽

Agroforestry Systems ◽

Chlorophyll B ◽

Total Chlorophyll ◽

Integration System ◽

Total Chlorophyll Content ◽

Urochloa Brizantha

The aim of this work was to study the effect of sub-doses of herbicide nicosulfuron on the levels of chlorophyll and growth of marandu-grass in an forest-livestock integration system. The treatments consisted of eucalyptus consortium with sorghum (BRS 655 hybrid) and forage Urochloa brizantha cv. Marandu (marandu-grass) at 12×2 and 12×3 m spacings in agroforestry systems, in addition to sorghum + marandu-grass and marandu-grass in monoculture. The doses of nicosulfuron applied at the marandu-grass were 0, 15 and 30 g ha-1. At 30 and 120 days after the application of nicosulfuron, chlorophyll a and chlorophyll b were measured usinf a portable chlorophyll meter, with six measurements per plant. Afterwards, the total chlorophyll content and chlorophyll a/b were calculated. We also measured the mass of the total dry matter of marandu-grass and growth rate of the culture. Marandu-grass plants cultivated in monoculture showed higher quantities of chlorophyll and the highest growth rates. The levels of chlorophyll a, b and chlorophyll a/b and the growth rate of marandu grass were similar between systems integrated with eucalyptus (12x2 and 12x3 m). The increase of herbicide dose negatively influenced on chlorophyll content in marandu-grass in all treatments evaluated. The use of nicosulfuron in doses of 25 and 50 g L-1 reduced the growth rate of marandu grass only when grown in monoculture

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EVALUATION OF SALINITY STRESS EFFECTS ON CHANGES IN PHOTOSYNTHETIC PIGMENTS, HYDROGEN PEROXIDE AND OSMOLYTES IN SENSITIVE AND TOLERANT CULTIVARS OF WHEAT CROP

PLANT ARCHIVES ◽

10.51470/plantarchives.2021.v21.s1.373 ◽

2020 ◽

Vol 21 (supplement 1) ◽

Author(s):

Rasoul Khodavirdivand Keshtiban ◽

Hassan Soltanloo ◽

Seyedeh Sanaz Ramazanpour ◽

Vahid Shariati ◽

Vahid Shariati

Keyword(s):

Hydrogen Peroxide ◽

Chlorophyll A ◽

Salinity Stress ◽

Chinese Spring ◽

Sampling Time ◽

Wheat Crop ◽

Chlorophyll B ◽

Total Chlorophyll ◽

Wheat Cultivars ◽

Total Carbohydrates

Investigation of wheat response to salinity stress can help to better understand the effective defense mechanisms of salinity stress tolerance. For this purpose, biochemical and physiological traits related to salinity tolerance in wheat cultivars were evaluated at Gorgan University of Agricultural Sciences and Natural Resources in 2019. Experimental factors, included two wheat crop cultivars (Sarc and Chinese spring as tolerant and susceptible wheat cultivars, respectively) and sampling time series (zero or control, 24, 48, 72, and 96 h) were examined in a factorial experiment based on a completely randomized design with three replications. Salinity stress was applied with sodium chloride at a concentration of 250 mM to uniform 10-day-old seedlings at the two-leaf stage, followed by sampling of shoot tissue. The studied traits were hydrogen peroxide (H2O2 ), chlorophyll a, chlorophyll b, total chlorophyll, chlorophyllase, carotenoids, proline, and total carbohydrates. Results of analysis of variance (ANOVA) indicated significant effects of genotype, time, and interaction of genotype × time (except H2O2 and total carbohydrates) on all the studied traits. Results of interaction of genotype × time showed although the trend of changes in the studied traits, depending on the type of cultivar and the sampling time were different, but generally, the susceptible Chinese spring cultivar contained higher levels of chlorophyllase and carotenoids than the control time at the end of sampling time and also higher H2O2 levels than the Sarc tolerant cultivar, while the Sarc tolerant cultivar, on the other hand, contained higher levels of chlorophyll a, chlorophyll b, total chlorophyll, and proline than the control time at the end of sampling time and also greater total carbohydrates than the susceptible Chinese spring cultivar. The results confirm the higher capacity of the antioxidant defense system of Sarc tolerant cultivar than the susceptible Chinese spring cultivar. Therefore

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Antioxidant properties of pepper (Capsicum annuum L.) depending on its cultivar and fruit colouration

Plant Soil and Environment ◽

10.17221/333/2021-pse ◽

2021 ◽

Author(s):

Agnieszka Żurawik ◽

Dorota Jadczak ◽

Nikolay Panayotov ◽

Piotr Żurawik

Keyword(s):

Antioxidant Activity ◽

Vitamin C ◽

Chlorophyll A ◽

Antioxidant Properties ◽

Temperate Climate ◽

Chlorophyll B ◽

Total Chlorophyll ◽

Climate Conditions ◽

Biological Value ◽

Fruit Colouration

Due to its nutritional, health-promoting and taste-related values, new cultivars are introduced every year. The aim of the study was to assess the biological value of Polish and Bulgarian cultivars of pepper grown in moderate climate conditions and collected at different degrees of maturity. Ascorbic acid, carotenoids, polyphenols, chlorophyll a, chlorophyll b, total chlorophyll and antioxidant activity (DPPH) were determined in air-dried fruit. The investigation included five Polish cultivars: Roberta, Marta Polka, Etiuda, Trapez, and Cyklon and five Bulgarian cultivars: Bulgarski Ratung, Sivriya, Kurtovska Kapiya, Delikates, and Dzuliunska Shipka. Its design involved randomised sub-blocks, with three replications comprising nine plants each. The area of a single plot was 1.44 m<sup>2 </sup>(1.2 m × 1.2 m). The study confirmed the strong antioxidant properties of pepper grown in the field, without cover, and under temperate climate conditions. The vegetable is rich in vitamin C, polyphenols, carotenoids, chlorophyll pigments, and shows high antioxidant activity. However, the biological value of pepper is cultivar-dependent and is also determined by the fruit colouration degree. The coloured fruits are richer in vitamin C and carotenoids than the green ones, and when matured, they have greater antioxidant capacity. Green fruits contain more chlorophyll a, chlorophyll b and total chlorophyll than the coloured ones. The fruits of cv. Etiuda were the richest in vitamin C, of cv. Sivriya in polyphenols, of cv. Dzuliunska Shipka in carotenoids, and of cv. Trapez in total chlorophyll. Cv. Sivriya showed the strongest antioxidant properties.

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Acifluorfen Tolerance inLycopersicon

Weed Science ◽

10.1017/s0043174500051833 ◽

1992 ◽

Vol 40 (3) ◽

pp. 413-417 ◽

Cited By ~ 4

Author(s):

Jacqueline A. Ricotta ◽

John B. Masiunas

Keyword(s):

Free Radical ◽

Chlorophyll A ◽

Negative Correlation ◽

Surface Characteristic ◽

Chlorophyll B ◽

Total Chlorophyll

Studies were conducted to determine the mechanism of acifluorfen tolerance within theLycopersicongenus. Absorption of14C-acifluorfen was not correlated with tolerance. There was a negative correlation (r = −0.57) between absorption 24 h after treatment and wax density. No other surface characteristic correlated with absorption. Less than 3% of absorbed14C was translocated and there was no metabolism of acifluorfen. All genotypes were susceptible to paraquat, and acifluorfen-tolerant genotypes had lower levels of ascorbate than susceptible genotypes, implying that free radical protectant systems were not involved in tolerance. Genotypes varied in amounts of chlorophyll a, chlorophyll b, and total chlorophyll but the differences did not correlate to acifluorfen tolerance.

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Synthesis, Characterization and Herbicidal Activity of Amide Derivatives of Glyphosate

Oriental Journal Of Chemistry ◽

10.13005/ojc/340519 ◽

2018 ◽

Vol 34 (5) ◽

pp. 2378-2383 ◽

Cited By ~ 1

Author(s):

Jatinder Pal Kaur Gill ◽

Nidhi Sethi ◽

Anand Mohan

Keyword(s):

Chlorophyll Content ◽

Chlorophyll A ◽

Structure Analysis ◽

Herbicidal Activity ◽

Chlorophyll B ◽

Total Chlorophyll ◽

Total Chlorophyll Content ◽

Amide Derivatives ◽

The Common ◽

Derivatives Of

The present work includes synthesis of a series of amide derivatives of glyphosate and their characterization. The structure analysis of these new derivatives was done with the help of FTIR and 1H NMR, Further, their herbicidal activity was analyzed on one of the common weeds (Parthenium hysterophorus). Under the influence of amide derivatives of glyphosate it was found that the chlorophyll content (Chlorophyll a, Chlorophyll b and total Chlorophyll content) of the weed was found to lessen than the control. Moreover, these synthesized derivatives are less polar as compared to the parent glyphosate molecule thereby can emphatically reduce the problem of their leaching into the groundwater.

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The Qualitative Differences for Photosynthetic Content of Common Bean (Phaseolus vulgaris L.) Populations in Kosovo

Notulae Scientia Biologicae ◽

10.15835/nsb639283 ◽

2014 ◽

Vol 6 (3) ◽

pp. 331-334

Author(s):

Sali Ali ALIU ◽

Imer RUSINOVCI ◽

Shukri FETAHU ◽

Bekim GASHI

Keyword(s):

Common Bean ◽

Chlorophyll A ◽

Room Temperature ◽

Genetic Material ◽

Carotenoid Content ◽

Chlorophyll B ◽

Total Chlorophyll ◽

Breeding Programs ◽

Chl A ◽

Acetone Water

Genetic diversity analysis of common bean populations is useful for breeding programs, as it helps to select genetic material to be used for further crossings. Twenty (20) common bean populations were analyzed using qualitative traits, chlorophyll “a” (Chl ‘a’), chlorophyll “b” (Chl ‘b’), total chlorophyll “a+b” (Total Chl) and carotenoides. The design of the experiment was conducted with leaves of common bean collected from different regions of Kosovo. The experiment was completely randomly with four repetitions. Pigments were extracted by grinding 80-100 mg freshly sampled leaves in 80% (v/v) acetone/water containing MgCO3, at room temperature, preserved in the dark for 24 hours. Concentration of chlorophyll and carotenoid content was measured by spectrophotometer using absorbance recorded at 663 nm, 644 nm and 452.3 nm for maximum absorption of Chl ‘a’, Chl ‘b’, and carotenoids respectively. According to our data the differences between populations for Chl ‘a’, and Chl ‘b’ was significantly higher at level of probability LSDp=0.01. The average values for Chl ‘a’, was 1.67 mg.g-1, while for Chl‘b’was 0.74 mg.g-1. In addition, the results for carotenoids content between populations were with high differences.

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Effect Different Concentrations of Crude Oil on the Pigment Content and Protein Content of Hydrilla Verticillata Plant

Iraqi Journal of Science ◽

10.24996/ijs.2019.60.10.6 ◽

2019 ◽

pp. 2141-2148

Author(s):

Sadiq Kadhum Lafta Alzurfi ◽

Kasim Kadhim Alasedi ◽

Noor Imad Abdulraheem

Keyword(s):

Statistical Analysis ◽

Crude Oil ◽

Protein Content ◽

Chlorophyll A ◽

Hydrilla Verticillata ◽

Gradual Decrease ◽

Control Treatment ◽

Chlorophyll B ◽

Total Chlorophyll ◽

Probability Level

The current study was conducted to determine the effect of crude oil on the pigments and protein content of Hydrilla verticillata plant from December 2018 to February 2019. Hydrilla verticillata was exposed to different concentrations of treatments (0.01,0.05,0.10,0.20) % of crude oil for 24 days. The pigments content was evaluated as total chlorophyll, a, b, carotene and protein content within plant tissues during the days (1, 8, 16 and 24). The results of this study showed an increment in the total chlorophyll values of plant, which is exposed to different concentrations of crude oil compared to the control treatment during the first day of the experiment and decreased compared to control treatment during 8,16 and 24 days of the experiment. The recorded maximum value of total chlorophyll was (29.49) Î¼g / g at 0.10% treatment during the first day and the statistical analysis showed significant differences below the probability level (P> 0.05) between the concentrations and the days of the experiment. Chlorophyll a, b and carotene of plant exhibit the overall trend of total chlorophyll. The values of chlorophyll b were higher than the values of chlorophyll and carotene. The current study showed a gradual decrease in the total protein values of plants in crude oil during experiential period compared to control treatment, recording the highest values at the 0.01% during the first day (29.4 mg/g) and the lowest in the concentration of 0.05% during the day 24 was (3.5) mg / g, and the statistical analysis showed significant differences below the probability level (P> 0.05) between the days and concentrations of the experiment. The study concluded that a gradual decrease of total chlorophyll values during period experimental, recorded the chlorophyll b values higher compare to chlorophyll a and carotene. the gradual decrease of protein values with the increasing the concentration.

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Effect of IBA, NAA and their Combination on Rooting and Biochemical Parameters of Stem Cuttings in Barbados Cherry

International Journal for Research in Applied Sciences and Biotechnology ◽

10.31033/ijrasb.8.5.21 ◽

2021 ◽

Vol 8 (5) ◽

pp. 147-150

Author(s):

Alam Khan Samim ◽

B. S. Shivakumar ◽

Ganapathi M.

Keyword(s):

Chlorophyll Content ◽

Chlorophyll A ◽

Growth Regulators ◽

Biochemical Parameters ◽

Root Initiation ◽

Stem Cuttings ◽

Chlorophyll B ◽

Total Chlorophyll ◽

Fresh Weight ◽

Total Chlorophyll Content

An investigation was to assess the influence of growth regulators IBA, NAA and their combination on rooting and biochemical parameters of stem cuttings in Barbados cherry. It was observed that, the least number of days (21.33) taken for root initiation, maximum percentage of rooting (80.00) was observed in cutting treated with IBA 5000 ppm followed by IBA 4500 ppm over other treatments. Significantly highest Chlorophyll-a (1.26), Chlorophyll –b (0.55) and total chlorophyll content (1.82 mg/g of fresh weight) of leaves and C : N ratio (8.74) was observed in cuttings treated with IBA 5000 ppm followed by IBA 4500 ppm over all other treatments.

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