scholarly journals Lignocellulosic Processing with Acid Pretreatment and Enzymatic Hydrolysis for Improving the Acquisition of Sugar Fermentation

2015 ◽  
Vol 9 (7) ◽  
pp. 24 ◽  
Author(s):  
Nuniek Hendrianie ◽  
Sri Rahmania Juliastuti ◽  
Moch. Izati Iwani ◽  
Affrida Eka
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Aspergillus Niger ◽  
Trichoderma Reesei ◽  
Lignin Content ◽  
Cellulose Content ◽  
Acid Pretreatment ◽  
Glucose Content ◽  
Mixture Ratio ◽  
Glucose Levels ◽  
Sugar Fermentation

Banana peels is a waste which has not been widely used, so that the relatively long time the existence of such waste brings its own problems such as pollution. Banana peels contain high enough lignocellulosic and can be degraded into simpler forms. The Lignin content of banana peels needs to be removed / destroyed structure. The purpose of this study was to determine the effect of the addition ratio of Trichoderma reseei and Aspergillus niger on hydrolysis liquefaction and saccharification time in stage hydrolysis to glucose produced with the highest glucose parameters. In this study, the method was used to degrade lignin by using sulfuric acid (2%). Afterward, the cellulose content was changed in liquefaction process. Hydrolysis liquefaction performed using Aspergillus niger and Trichoderma reesei with a mixture ratio of 1: 0, 0: 1, 1: 1 and 1: 2. Furthermore, substrate hydrolysis liquefaction saccharification going through the process of hydrolysis to increase glucose levels were formed. Hydrolysis liquefaction aims to change the content of starch in the banana skin into glucose using Saccharomyces cerevisiae as the addition of 20% (v / v). Hydrolysis process of liquefaction is done by varying the sampling time on day - 3, 6, and 9. In this study the hydrolysis of liquefaction of banana peels use a mixture of Trichoderma reesei and Aspergillus niger 2:1 at a temperature of 50oC, pH 5, and 64 h for resulted in glucose with the best content was 0.52%. In addition, the results showed that the hydrolysis saccharification with the addition of Saccharomyces cerevisiae glucose levels were highest on day 3 with a temperature of 30oC and pH 5, resulting in glucose content to 1.63%.

Lignocellulosic Processing with Acid Pretreatment and Enzymatic Hydrolysis for Improving the Acquisition of Sugar Fermentation

2015 ◽  
Vol 9 (7) ◽  
pp. 23 ◽  
Author(s):  
Nuniek Hendrianie ◽  
Sri Rahmania Juliastuti ◽  
Moch. Izati Iwani ◽  
Affrida Eka
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Aspergillus Niger ◽  
Trichoderma Reesei ◽  
Lignin Content ◽  
Banana Peel ◽  
Acid Pretreatment ◽  
Glucose Content ◽  
Mixture Ratio ◽  
Glucose Levels ◽  
Sugar Fermentation

<p class="zhengwen"><span lang="EN-GB">Banana peel</span><span lang="IN">s</span><span lang="EN-GB"> is a waste </span><span lang="IN">which</span><span lang="EN-GB"> has not been widely used, so that the relatively long time the existence of such waste brings its own problems such as pollution. Banana peels contain high enough lignocellulosic and can be degraded into simpler forms.</span><span lang="IN"> The</span><span lang="EN-GB"> Lignin content </span><span lang="IN">of</span><span lang="EN-GB"> banana </span><span lang="IN">peels</span><span lang="EN-GB"> needs to be removed / destroyed structure. The purpose of this study was to determine the effect of the addition ratio of <em>Trichoderma reseei </em>and <em>Aspergillus niger</em> on hydrolysis liquefaction and saccharification time in stage hydrolysis to glucose produced with the highest glucose parameters. In this study, the method</span><span lang="IN"> was</span><span lang="EN-GB"> used to degrade lignin </span><span lang="IN">by using sulfuric acid</span><span lang="EN-GB"> (2%). After</span><span lang="IN">ward,</span><span lang="IN"> </span><span lang="EN-GB">the</span><span lang="IN"> cellulose</span><span lang="EN-GB"> content </span><span lang="IN">was changed in </span><span lang="EN-GB">liquefaction </span><span lang="IN">process</span><span lang="EN-GB">. Hydrolysis liquefaction performed using <em>Aspergillus niger</em> and <em>Trichoderma reesei</em> with a mixture ratio of 1: 0, 0: 1, 1: 1 and 1: 2. Furthermore, substrate hydrolysis liquefaction saccharification going through the process of hydrolysis to increase glucose levels were formed. Hydrolysis liquefaction aims to change the content of starch in the banana skin into glucose using Saccharomyces cerevisiae as the addition of 20% (v / v). Hydrolysis process of liquefaction is done by varying the sampling time on day - 3, 6, and 9. In this study the hydrolysis of liquefaction of banana peels use a mixture of <em>Trichoderma reesei</em> and <em>Aspergillus niger</em> 2:1 at a temperature of 50<sup>o</sup>C, pH 5, and 64 h for resulted in glucose with the best content was 0.52%. In addition, the results showed that the hydrolysis saccharification with the addition of <em>Saccharomyces cerevisiae</em> glucose levels were highest on day 3 with a temperature of 30<sup>o</sup>C and pH 5, resulting in glucose content to 1.63%.</span></p>

scholarly journals Synthesis of lactic acid from sugar palm trunk waste (Arenga pinnata): Preliminary hydrolysis and fermentation studies

2020 ◽  
Vol 21 (5) ◽  
Author(s):  
WHINY HARDIYATI ERLIANA ◽  
Tri Widjaja ◽  
ALI ALTWAY ◽  
LILY PUDJIASTUTI
Keyword(s):  
Lactic Acid ◽  
Lignin Content ◽  
Agricultural Waste ◽  
Lactic Acid Production ◽  
Lactobacillus Rhamnosus ◽  
Lactobacillus Brevis ◽  
Reducing Sugar ◽  
Cellulose Content ◽  
Acid Pretreatment ◽  
Acid Fermentation

Abstract. Erliana WH, Widjaja T, Altway A, Pudjiastuti L. 2020. Synthesis of lactic acid from sugar palm trunk waste (Arenga pinnata): Hydrolysis and fermentation studies. Biodiversitas 21: 2281-2288. The increasing problems of global energy and the environment are the main reasons for developing products with new techniques through green methods. Sugar palm trunk waste (SPTW) has potential as agricultural waste because of its abundant availability, but it is not used optimally. This study was aimed to determine the effect of various microorganisms on increasing lactic acid production by controlling pH and temperature conditions in the fermentation process. SPTW contains 43.88% cellulose, 7.24% hemicellulose, and 33.24% lignin. The lignin content in SPTW can inhibit reducing sugar formation; the pretreatment process should remove this content. In the study, the pretreatment process was conducted using acid-organosolv. In the acid pretreatment, 0.2 M H2SO4 was added at 120oC for 40 minutes; organosolv pretreatment using 30% ethanol (v/v) at 107oC for 33 minutes was able to increase cellulose content by 56.33% and decrease lignin content by 27.09%. The pretreatment was followed by an enzymatic hydrolysis process with a combination of commercial cellulase enzymes from Aspergillus niger (AN) and Trichoderma reesei (TR), with variations of 0:1, 1:0, 1:1, 1:2 and 2:1. The best reducing sugar concentration was obtained with an AN: TR ratio of 1:2 to form reducing sugar from cellulose. Subsequently, lactic acid fermentation was carried out using lactic acid bacteria at 37oC and pH 6 incubated for 48 hours. The highest lactic acid concentration (33.292 g/L) was obtained using a mixed culture of Lactobacillus rhamnosus and Lactobacillus brevis to convert reducing sugar become lactic acid.

scholarly journals KINERJA MIKROBA Zymomonas mobilis DAN Saccharomyces cerevisiae UNTUK MENGURAIKAN HIDROLISAT TONGKOL JAGUNG MENJADI BIOETANOL DENGAN PENGARUH WAKTU FERMENTASI DAN RASIO PENAMBAHAN MIKROBA

2017 ◽  
Vol 6 (2) ◽  
pp. 1-6
Author(s):  
Fatimah ◽  
Deralisa Ginting ◽  
Veronica Sirait
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Gas Chromatography ◽  
Aspergillus Niger ◽  
Trichoderma Reesei ◽  
Zymomonas Mobilis ◽  
Alternative Fuel ◽  
Fermentation Time ◽  
Corn Cobs ◽  
Hydrolysis Of ◽  
Bioethanol Yield

Bioethanol from biomass is one of energy which  has a  potential as  alternative fuel. Bioethanol can be produced by using fungi or bacteria. The research was about  the performance of Zymomonas mobilis and Saccharomyces cerevisiae to change corn cobs hydrolyzate into bioethanol by adding microbes to the influence of time and ratio of  fermentation had been done. The hydrolyzate  were decomposition of corn cobs using Trichoderma reesei and Aspergillus niger. The purpose of this study was to know the conversion of  hydrolysis of corn cobs into bioethanol with variation time of fermentation (1 day, 3 days, 5 days, and 7 days) and rate of adding microbes  (Zymomonas mobilis : Saccharomyces cerevisiae = 1:1; 1:2 and 2:1) (v/v). The glucose from corn cobs hydrolyzate was 5,869 g/ml. Fermentation wass carried out at 25 0C. Bioethanol which obtained from this study was investigated using gas chromatography. The optimum bioethanol yield was equal to 6,31% by using Zymomonas mobilis : Saccharomyces cerevisiae (2:1) and at the 3 days  fermentation time.

scholarly journals Produksi Etanol Berbahan Baku Molasses Melalui Proses Fermentasi Menggunakan Ragi Roti

2021 ◽  
Vol 1 (1) ◽  
pp. 1
Author(s):  
Yustia Wulandari Mirzayanti ◽  
Sugiono . ◽  
Reta Kurniayati
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Renewable Energy ◽  
Ethanol Production ◽  
Incubation Time ◽  
Baker’S Yeast ◽  
Baker's Yeast ◽  
Alcohol Content ◽  
Glucose Content ◽  
Alcohol Production ◽  
Glucose Levels

<table class="NormalTable"><tbody><tr><td width="200"><span class="fontstyle0">One of the alternatives and renewable energy that is being developed is ethanol.<br />Ethanol is better known as Gasohol. Molasses can make Gasohol through the<br />synthesis of molasses fermentation using a yeast starter. This study aims to<br />analyze how the effect of the addition of baker's yeast, the length of fermentation<br />incubation time on the resulting alcohol content. In addition, the yield obtained<br />from the highest amount of ethanol production in the molasses fermentation<br />process. Ethanol production through fermentation synthesis using the help of<br />microorganisms Saccharomyces cerevisiae. Based on the objective review, the<br />variation used is the amount of baker's yeast, namely 0.1; 0.2; 0.3; 0.4; and 0.5%<br />glucose levels. The fermentation times were 24, 48, 72, 96, and 120 hours (T =<br />30?C and pH = 5). Based on these variations, the highest alcohol production<br />was 11%, obtained by adding 0.2% of yeast to the glucose content in the solution.<br />The incubation time is 72 hours. The yield obtained for the highest alcohol<br />content is 4.48%</span></td></tr></tbody></table>

scholarly journals Microbial production of 2,3-butanediol from rice husk using anaerobic Clostridium species

2021 ◽  
Vol 15 (1) ◽  
pp. 251-262
Author(s):  
A. Sanusi ◽  
A.A. Farouq ◽  
A.Y. Bazata ◽  
A.D. Ibrahim ◽  
I. Mas’ud ◽  
...  
Keyword(s):  
Aspergillus Niger ◽  
Rice Husk ◽  
Lignin Content ◽  
Agricultural Waste ◽  
Major Fatty Acid ◽  
Reducing Sugar ◽  
Microbial Production ◽  
Gas Chromatography Mass Spectrometry ◽  
Cellulose Content ◽  
Clostridium Species

Interest in the area of biomass based-product production is increasing all over the world due to the environmental challenges posed by fossil fuel and fear of its extinction. Production of biofuel and other compounds especially from agricultural waste can reduce these environmental problems because of its sustainability and environmentally friendliness. One of the major petrochemical product widely used in many industries is 2,3-butanediol and was found to be produced from agricultural wastes by microorganisms. Therefore, Microbial production of 2,3-butanediol from rice husk using Clostridium species was investigated in this research. Structural composition of the rice husk was determined before and after pretreatment. Hemicellulose and lignin content of rice husk was determined after extraction while cellulose was determined as the difference from the extractives, hemicelluloses and lignin. Dilute (2%) NaOH was used for the pretreatment of rice husk. Hydrolysis was carried out using Aspergillus niger and reducing sugar released was determined using standard method with UV-VIS spectrophotometer. Clostridium species was isolated from sugarcane bagasse, identified using basic morphological and molecular biology techniques. The fermentation of rice husk was performed using the Clostridium species. Fermentation by-product was determined using Gas Chromatography Mass-spectrometry. Cellulose content increased from 32% before pretreatment to 53.3% after pretreatment, lignin increased from 8.4% before pretreatment to 30.7% after pretreatmemt and hemicellulose decreased from 30% before pretreatment to 8% after pretreatment. A total of 1.05 g/l of reducing sugar was released after enzymatic hydrolysis of the rice husk with Aspergillus niger. Alcohol 2,3-butanediol (0.6%) and Furfuryl alcohol (0.45%) were detected in the by-product of fermentation. Other compounds detected are fatty acids that ranges from C16 to C25 with 9,12-Octadecanoic acid as the major fatty acid. From the results of this work, Rice husk was found to have substantial amount of sugar (cellulose and hemicelluce) that can be converted to valuable product including 2,3-butanediol. Keywords: Biofuel, Bio-refinary, Cellulose, Clostrudium, fermentation.

scholarly journals Effect of Dilute Acid - Alkaline Pretreatment on Rice Husk Composition and Hydrodynamic Modeling with CFD

2019 ◽  
Vol 4 (1) ◽  
pp. 18
Author(s):  
Novia Novia ◽  
Vishnu K Pareek ◽  
Hermansyah Hermansyah ◽  
Asyeni Miftahul Jannah
Keyword(s):  
Rice Husk ◽  
Lignin Content ◽  
Hydrodynamic Modeling ◽  
Alkaline Pretreatment ◽  
Hot Water ◽  
Dilute Acid Pretreatment ◽  
Cfd Modeling ◽  
Cellulose Content ◽  
Dilute Acid ◽  
Acid Pretreatment

The high cellulosic content of rice husk can be utilized as a feedstock for pulp and biofuel. Pretreatment is necessary to break the bonds in the complex lignocellulose matrices addressing the cellulose access. This work aims to utilize the rice husk using dilute acid and alkaline pretreatment experimentally and CFD modeling. The study consists of three series of research. The first stage was the dilute acid pretreatment with sulfuric acid concentration of 1% to 5% (v/v) at 85°C for 60 minutes, and alkaline pretreatment with NaOH concentration of 1% to 5% (w/v) at 85oC for 30 minutes separately. The second stage used the combination of both pretreatment. Moreover the last stage of research was hydrodynamic modeling of pretreatment process by CFD (ANSYS FLUENT 16). The experimental results showed that the lowest lignin content after acid pretreatment was about 10.74%. Alkaline pretreatment produced the lowest lignin content of 4.35%. The highest cellulose content was 66.75 % for acid-alkaline pretreatment. The lowest content of lignin was about 6.09% for acid-alkaline pretreatment. The lowest performance of alkaline pretreatment on HWS (hot water solubility) of about 7.34% can be enhanced to 9.71% by using a combination alkaline-acid. The combined pretreatments result hemicellulose of about 9.59% (alkaline-acid) and 9.27% (acid-alkaline). Modeling results showed that the mixing area had the minimum pressure of about -6250 Pa which is vortex leading minimum efficiency of mixing. The rice husk flowed upward to the upper level and mixed with reagent in the perfect mixing.  

scholarly journals Lignin and Cellulose Content of Fermented Rice Straw with Aspergillus niger (van Tieghem) and Trichoderma mutan AA1

2021 ◽  
Vol 226 ◽  
pp. 00043
Author(s):  
Sri Sukaryani ◽  
Engkus Ainul Yakin ◽  
Yos Wahyu Harinta ◽  
Zane Vincēviča–Gaile ◽  
Endang Dwi Purbajanti
Keyword(s):  
Aspergillus Niger ◽  
Rice Straw ◽  
Crude Protein ◽  
Lignin Content ◽  
Silica Content ◽  
Limiting Factors ◽  
Cellulose Content ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Level 1

The rice straw has potential to be used as an alternative ruminant feed. However, it has limiting factors i.e low crude protein, high crude fiber, lignin, cellulose, and silica content. To overcome the limiting factors, immersion in a solution of alkaline (lime) or fermentation by using inoculum microbial cellulolytic and lignocellulolytic (Trichoderma mutan AA1 and Aspergillus niger.). The research method was experimental, with four treatments and repeated five times. Completely randomized design was used and if there are differences among treatments a further test with DMRT was carried out (level 1 % and 5 %). These treatments were T0: The rice straw without t fermentation; T1: Fermented with A. niger; T2: Fermented with T. mutan AA1; T3: Fermented with a combination A. niger and T. mutan AA1. The results showed that the rice straw fermented with A. niger and T. mutants AA1 very significantly increased the cellulose and decreased lignin content. The highest cellulose reached on T3 (20.297 %) followed by T2 (18.191 %), T1 (17.712 %) and T0 (16.747 %), respectively. While the lowest content of lignin reached on T3 (14.793 %), followed T2 (26.063 %), T1 (26.421 %) and T0 (38.164 %).

scholarly journals Bioethanol production of second generation from corn cob

2021 ◽  
pp. 29-33
Author(s):  
Fabiola Sandoval-Salas ◽  
Carlos Méndez-Carreto ◽  
Christell Barrales-Fernández ◽  
Graciela Ortega-Avila
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Carbon Source ◽  
Second Generation ◽  
Culture Media ◽  
Lignin Content ◽  
Cellulose Content ◽  
Lignocellulosic Materials ◽  
Bioethanol Production ◽  
Corn Cob ◽  
Theoretical Yield

Bioethanol production from lignocellulosic materials has several environmental and economic advantages. In this work, corn cob was used to produce ethanol by fermentation. The cob was grounded, hydrolyzed chemically, and then enzymatically. Later, hydrolysates were used as a carbon source to formulate culture media that were inoculated with Saccharomyces cerevisiae; hollocellulose content was quantified by the ASTM D-1104 method; cellulose content by the TAPPTI 212 method; lignin content by the NREL / TP-510-42618 method; and ethanol was quantified by HPLC. In fermentation, bioethanol yields of up to 3.5 g / L were found, equivalent to YP/S value of 0.46, representing approximately 90% of the theoretical yield.

Analysis of Sound Reduction and Strong Drag Composite Concrete Fibers Gedebok Banana Results Delignification with Sodium Hydroxide Solvent (Naoh)

2018 ◽  
Vol 6 (02) ◽  
pp. 105-120
Author(s):  
Muhammad Rouf Suprayogi ◽  
Annisa Mufida ◽  
Edwin Azwar
Keyword(s):  
Tensile Strength ◽  
Compressive Strength ◽  
Lignin Content ◽  
Sound Intensity ◽  
Cellulose Fiber ◽  
Cellulose Content ◽  
Cellulose Powder ◽  
Drying Method ◽  
Normal Concrete ◽  
Sound Reduction

In composite science, desirable materials that are lighter but have the power and quality that can match or even exceed the material that has been there before. The purpose of this study was to investigate the effect of cellulose fiber addition from banana gedebok to tensile strength, compressive strength and damping of concrete composite sound. To achieve this objective, mixing of cellulose fibers with K-275 quality concrete mix with variation of 0% and 5% substitution in which the cellulose is varied in powder and wicker form. Delignification of lignin content from banana gedebok was done by soaking and drying method without any variation and yielding powder having cellulose content of 13,0388%, hemicellulose 18,2796% and lignin 0,6684%. This study produces concrete composites that have a tensile strength and a compressive strength lower than that of normal concrete. Normally reinforced concrete tensile strength value 94.5 kg / cm2, 71.4 kg / cm2 cellulose powder concrete and 90.3 kg / cm2 cellulose woven concrete. Normal concrete compressive strength value 334,22 kg / cm2, cellulose powder concrete 215,7 kg / cm2, and cellulose webbing concrete 157,98 kg / cm2. As for the power damping sound of cellulose webbing concrete has the highest damping power compared to other concrete with the absorbed sound intensity that is 52-68 dB

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