Morphological Characterization of Mulberry (Morus spp.) Accessions Grown in Kenya

Genetic divergence of five mulberry accessions including Embu, Thika, Thailand (M. alba), Kanva-2 and S41 (M. indica) grown in Kenya were examined using twelve phenotypic traits. The assessment of phenotypic traits was done in a field study in two localities, Nairobi and Eldoret. The traits that were significantly different across the mulberry accessions included lamina width and petiole length (P ? 0.01), petiole width and growth height (P ? 0.05), internodes distance and number of branches (P ? 0.001). The Duncan’s Multiple Range Test (DMRT) results were used to generate a dendrogram derived from hierarchical cluster analysis that further partitioned the mulberry accessions into four groups. Embu and Thailand accessions grouped together while S41, Thika and Kanva-2 accessions grouped separately. Embu and Thailand accessions were characterized by fewer numbers of branches than the rest of the accessions. Thika accession had high number of branches and short internode distance. Significant and positive correlations were found between leaf yield traits except in internode distance and number of branches which were significant and negatively correlated. Significant and positive correlations can be utilized since they are rewarding for mulberry leaf yield improvement.

Download Full-text

Morphometric Characterization of the Lidia Cattle Breed

Animals ◽

10.3390/ani10071180 ◽

2020 ◽

Vol 10 (7) ◽

pp. 1180

Author(s):

Juan Manuel Lomillos ◽

Marta E. Alonso

Keyword(s):

Cattle Breed ◽

Internal Variability ◽

Morphological Characterization ◽

Relative Depth ◽

Sexually Dimorphic ◽

Muscular Development ◽

Morphological Differences ◽

Positive Correlations ◽

Morphometric Characterization

Morphometric studies in Lidia cattle are scarce due to the challenges of handling and approaching this breed of cattle. For this reason, the first morphological characterization of the Lidia breed was carried out using photogrammetry. In the present work, 264 adult individuals (184 males and 80 females), belonging to 21 different herds, were studied. A total of 20 morphological measurements and five indexes were determined in every individual. There were many positive correlations between the measures, giving the model great morphostructural harmony. Considerable internal variability of the studied parameters was observed. This breed reflected significant sexually dimorphic features and internal morphological differences between the different genetic lines of the breed. Lidia cattle are small and mostly have a sub-concave profile (58.4% males and 69.7% females). The male proportionality indexes and the relative depth of the thorax indicated great muscular development of the anterior third and high thoracic capacity. The phaneroptic information describes a mostly black animal with black mucous and hooves and an outstanding development of the dewlaps and the tail in the males.

Download Full-text

PHENOTYPIC CHARACTERIZATION OF BENECKEA PARAHAEMOLYTICA: A PRELIMINARY REPORT1

Journal of Milk and Food Technology ◽

10.4315/0022-2747-36.4.214 ◽

1973 ◽

Vol 36 (4) ◽

pp. 214-219 ◽

Cited By ~ 6

Author(s):

Paul Baumann ◽

Linda Baumann

Keyword(s):

Liquid Medium ◽

Type Strain ◽

Solid Medium ◽

Morphological Characterization ◽

The Other ◽

Polar Flagellum ◽

Phenotypic Characterization ◽

Phenotypic Traits ◽

General Properties

Eighty-six strains which were isolated from cases of gastroenteritis and had the general properties of the genus Beneckea were submitted to an extensive nutritional, physiological, and morphological characterization. The results indicated that this collection of strains, which included the type strain of Beneckea parahaemolytica, was phenotypically homogeneous and distinguishable from the other known species of Beneckea by multiple, unrelated, phenotypic traits. When grown in liquid medium, strains of B. parahaemolytica had single, sheathed, polar flagella; when grown on solid medium, these strains had unsheathed, peritrichous flagella in addition to the sheathed, polar flagellum. Additional traits of use for differentiation of this species from the remaining species of the genus Beneckea were the ability of B. parahaemolytica to grow at 40 C, utilize d-galactose, l-leucine, l-histidine, and putrescine and the inability to utilize sucrose, dl-β-hydroxy-butyrate or give a positive Voges-Proskauer reaction. The validity of some of the traits previously used to identify B. parahaemolytica as well as the possible difficulties encountered in the identification of this organism from marine sources are considered.

Download Full-text

Morphological characterization of selected papaya (Carica papaya L.) inbreds and hybrids

Annals of Tropical Research ◽

10.32945/atr3116.2009 ◽

2009 ◽

pp. 102-112

Author(s):

Florence Lasalita-Zapico ◽

Violeta Villegas

Keyword(s):

Carica Papaya ◽

Genetic Relatedness ◽

Morphological Characteristics ◽

Morphological Characterization ◽

Accurate Estimation ◽

Phenotypic Traits ◽

Complete Dominance ◽

Phylogenetic Affinities ◽

High Degree

Morphological analysis was undertaken to assess the degree of genetic relatedness and to characterize selected papaya (Carica papaya L.) inbreds and hybrids. Transmission of phenotypic traits from inbred parents to hybrid progeny followed the Mendelian pattern (complete dominance). The clustering mechanism separated the papaya genotypes into two groups. It was also revealed that some inbred lines presumably of very diverse origins exhibited similar morphological characteristics, raising the possibility that they have phylogenetic affinities and/or common origins. Screening for morphological traits with a high degree of polymorphism and with invariable expressions of the phenotypes would help in the identification of markers for hybrid identification and also in the accurate estimation of genetic relatedness among these hybrids and their parents.

Download Full-text

Collection and agro morphological characterization of Algerian accessions of lentil (Lens culinaris)

Biodiversitas Journal of Biological Diversity ◽

10.13057/biodiv/d190125 ◽

2018 ◽

Vol 19 (1) ◽

pp. 183-193 ◽

Cited By ~ 1

Author(s):

DJOUHER GAAD ◽

MERIEM LAOUAR ◽

AISSA ABDELGUERFI ◽

FATIMA GABOUN

Keyword(s):

Lens Culinaris ◽

Quantitative Traits ◽

Hierarchical Cluster ◽

Morphological Characterization ◽

Ecological Zones ◽

Morphological Evaluation ◽

Quantitative Characters ◽

Arid Conditions ◽

Agro Ecological Zones

Gaad D, Laouar M, Gaboun F, Abdelguerfi A. 2018. Collection and agro morphological characterization of Algerian accessions of lentil (Lens culinaris). Biodiversitas 19: 183-193. Lentil is one of the important pulse crops in Algeria. The narrow genetic base of local cultivars and the disappearance of many local accessions contribute to the loss of lentil biodiversity. Therefore, collection, characterization and preservation of existing local accessions of lentils are important. Lentil accessions were collected across different agro-ecological zones of Algeria. From 10 regions (Departments), 15 villages were surveyed and 30 accessions were collected. Eighteen local accessions and 26 references collection have been used for agro morphological evaluation. The assessment was performed in two locations, sub-humid and semi-arid conditions, based on 12 quantitative characters. The result of variance analysis shows a significant effect of the interaction genotype x location for six quantitative traits. Three macrosperma Algerian accessions characterized by a high number of pod/plant, number of seed/plant and seed yield/plant were positioned on PCA1. The remaining Algerian accessions were positioned on PCA2 and were characterized by later flowering and maturity with high plant stature and height of the lowest pods. Hierarchical cluster analysis grouped the accessions into five distinct clusters independently of the accessions origins and revealed the distribution of Algerian accessions in all of the groups. This study will help breeders to better select accessions to be used in lentil breeding program.

Download Full-text

Morphological Characterization of Mycobacteriophage R1

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100051281 ◽

1974 ◽

Vol 32 ◽

pp. 254-255

Author(s):

B. L. Soloff ◽

T. A. Rado

Keyword(s):

Carbon Source ◽

Stationary Phase ◽

Growth Phase ◽

Minimal Medium ◽

Morphological Characterization ◽

Logarithmic Growth Phase ◽

Complete Lysis ◽

Lysogenic Culture ◽

Logarithmic Growth

Mycobacteriophage R1 was originally isolated from a lysogenic culture of M. butyricum. The virus was propagated on a leucine-requiring derivative of M. smegmatis, 607 leu−, isolated by nitrosoguanidine mutagenesis of typestrain ATCC 607. Growth was accomplished in a minimal medium containing glycerol and glucose as carbon source and enriched by the addition of 80 μg/ ml L-leucine. Bacteria in early logarithmic growth phase were infected with virus at a multiplicity of 5, and incubated with aeration for 8 hours. The partially lysed suspension was diluted 1:10 in growth medium and incubated for a further 8 hours. This permitted stationary phase cells to re-enter logarithmic growth and resulted in complete lysis of the culture.

Download Full-text

Morphological Characterization of Rhinacanthus Species of Sri Lanka

Planta Medica ◽

10.1055/s-0030-1251769 ◽

2010 ◽

Vol 76 (05) ◽

Author(s):

APPR Amarasinghe ◽

RP Karunagoda ◽

DSA Wijesundara

Keyword(s):

Sri Lanka ◽

Morphological Characterization

Download Full-text

Density and morphological characterization of bacteria in integrated and exclusive production systems

Scientific Electronic Archives ◽

10.36560/13920201194 ◽

2020 ◽

Vol 13 (11) ◽

pp. 1

Author(s):

A. R. B. Zanco ◽

A. Ferreira ◽

G. C. M. Berber ◽

E. N. Gonzaga ◽

D. C. C. Sabino

Keyword(s):

Bacterial Community ◽

Rainy Season ◽

Production Systems ◽

Cropping System ◽

Morphological Characterization ◽

Native Forest ◽

Exclusive Production ◽

Colony Forming Units

The different integrated production systems can directly interfere with its bacterial community. The present study aimed to assess density, bacterial diversity and the influence of dry and rainy season in different integrated and an exclusive production system. The fallow and a native forest area was assessed to. Samples were collected in 2012 March and September. The isolation were carried out into Petri dishes containing DYGS medium. The number of colony forming units (CFU) was counted after 48 hours and. The bacterial density ranged between 106 and 107 CFU g-1 soil. The crop system affected the dynamics of the bacterial community only in the rainy season. The rainy season showed greater density of total bacteria when compared to the dry period regardless of the cropping system. The dendrograms with 80 % similarity showed thirteen and fourteen groups in the rainy and dry seasons. Isolates with the capacity to solubilize phosphate in vitro were obtained from all areas in the two seasons, but this feature has been prevalent in bacteria isolated during the rainy season

Download Full-text

Characterization of Antibacterial-Producing Endophytic Fungi of Syzygiumpolyanthum Leaves

Infectious Disorders - Drug Targets ◽

10.2174/1871526519666181226123541 ◽

2020 ◽

Vol 20 (4) ◽

pp. 448-454

Author(s):

Rahmita Burhamzah ◽

Gemini Alam ◽

Herlina Rante

Keyword(s):

Molecular Characterization ◽

Endophytic Fungi ◽

Morphological Characterization ◽

Test Method ◽

Analysis Method ◽

Nitrogen Base ◽

Rdna Genes ◽

Antibacterial Screening ◽

Planting Method

Background: Endophytic fungi live in plants’ tissue and can produce the same bioactive compounds as its host plant produces. Syzygiumpolyanthum leaves have known to be one of the antibacterial compound producers. Aim and Objective: This study aimed to characterize morphologically, microscopically, and molecularly the antibacterial-producing endophytic fungi of Syzygiumpolyanthum leaves. Methods: The isolation of endophytic fungi was done by fragment planting method on PDA medium. The antibacterial screening was performed using the antagonistic test as the first screening followed by the disc diffusion test method. The morphological characterization was based on isolate’s mycelia color, growth pattern, margin, and surface texture of the colony, while the microscopic characterization was based on its hyphae characteristics. The molecular characterization of the isolate was done by nitrogen base sequence analysis method on nucleotide constituent of ITS rDNA genes of the isolate. Results: The results found that isolate DF1 has antibacterial activity against E.coli, S.aureus, P.acne, and P.aeruginosa, with the greatest inhibition at 10% concentration of broth fermentation extract on S.aureus with a diameter of inhibition of 13.77 mm. Conclusion: Based on macroscopic, microscopic, and molecular characterization, DF1 isolate is similar to Ceriporialacerate.

Download Full-text

Microscopic Image Segmentation and Morphological Characterization of Novel Chitosan/Silica Nanoparticle/Nisin Films Using Antimicrobial Technique for Blueberry Preservation

Membranes ◽

10.3390/membranes11050303 ◽

2021 ◽

Vol 11 (5) ◽

pp. 303

Author(s):

Rokayya Sami ◽

Schahrazad Soltane ◽

Mahmoud Helal

Keyword(s):

Ph Value ◽

Silica Nanoparticle ◽

Morphological Characterization ◽

Aerobic Bacteria ◽

Nano Materials ◽

Elongation At Break ◽

Stable Suspension ◽

Average Size ◽

Material Ductility

In the current work, the characterization of novel chitosan/silica nanoparticle/nisin films with the addition of nisin as an antimicrobial technique for blueberry preservation during storage is investigated. Chitosan/Silica Nanoparticle/N (CH-SN-N) films presented a stable suspension as the surface loads (45.9 mV) and the distribution was considered broad (0.62). The result shows that the pH value was increased gradually with the addition of nisin to 4.12, while the turbidity was the highest at 0.39. The content of the insoluble matter and contact angle were the highest for the Chitosan/Silica Nanoparticle (CH-SN) film at 5.68%. The use of nano-materials in chitosan films decreased the material ductility, reduced the tensile strength and elongation-at-break of the membrane. The coated blueberries with Chitosan/Silica Nanoparticle/N films reported the lowest microbial contamination counts at 2.82 log CFU/g followed by Chitosan/Silica Nanoparticle at 3.73 and 3.58 log CFU/g for the aerobic bacteria, molds, and yeasts population, respectively. It was observed that (CH) film extracted 94 regions with an average size of 449.10, at the same time (CH-SN) film extracted 169 regions with an average size of 130.53. The (CH-SN-N) film presented the best result at 5.19%. It could be observed that the size of the total region of the fruit for the (CH) case was the smallest (1663 pixels), which implied that the fruit lost moisture content. As a conclusion, (CH-SN-N) film is recommended for blueberry preservation to prolong the shelf-life during storage.

Download Full-text