scholarly journals Comparative study of some standardization parameters of two samples of Aquilegia sibirica L. aerial parts

2020 ◽  
pp. 20-25
Author(s):  
Nomin M ◽  
Odontuya G
Keyword(s):  
Quantitative Analysis ◽  
Total Phenolic ◽  
Control Parameters ◽  
Altai Mountains ◽  
Total Phenolic Compounds ◽  
Active Compounds ◽  
Aerial Parts ◽  
Geographical Regions ◽  
Two Samples ◽  
Total Moisture Content

The aerial parts of Aquilegia sibirica L. were prepared from two different plant-geographical regions of Mongolia. Some main qualitative control parameters and a quantitative analysis of main biological active compounds of these samples were studied for a standardization of this drug. The aerial parts of Aquilegia sibirica L. were collected on June 26, 2006 at the Altai mountains, steppe, Gobi-Altai aimag, Khantaishir range, Yalaatin am (N46о105’, E97о004’) and on June 23, 2019 at the Khyangan mountains, meadow, Selenge aimag, Mandalsoum, Thunkhel village (N48o63’, E106o79’). As a result of the study, the total moisture content of the samples collected from Gobi-Altai and Selenge aimags were 8.25 ± 0.31% and 8.45 ± 0.13%, the total ash was 8.85 ± 0.17% and 9.71 ± 0.21%, and the acid insoluble ash was 0.95 ± 0.05% and 0.99 ± 0.26%, the water-extracted compounds were 26.24 ± 0.85% and 25.67 ± 0.53%, respectively. According to the analysis of main biological active compounds of these samples, the total phenolic compounds 2.8 ± 0.05% and 2.5 ± 0.05% (gallic acid equivalents), the total flavonoids 3.6 ± 0.13% and 3.67 ± 0.06% (isocytisoside equivalents), and the total tannins 0.79 ± 0.03% and 0.55 ± 0.03% (pyrogallol equivalents) were determined, respectively. Study of qualitative control parameters and quantitative analysis of main biological active compounds of this plant drug collected from different regions gave comparable results. Сибирь удвал (Aquilegia sibirica L.) ургамлын газрын дээд хэсгийн хоёр дээжийн стандарчиллын зарим үзүүлэлтийн харьцуулсан судалгаа Хураангуй: Манай оронд ургадаг Сибирь удвал ургамлын газрын дээд хэсгийг ургамал газар зүйн ангиллын хоёр өөр бүс нутгаас бэлтгэсэн бөгөөд энэхүү эмийн түүхий эдийн стандартчиллын гол үзүүлэлт болох чанарын хяналтын тоон үзүүлэлтүүд, биологийн идэвхт гол нэгдлүүдийн тооны шинжилгээг харьцуулан судлав. Бид Сибирь удвал ургамлын газрын дээд хэсгийг 2006 оны 06-р сарын 26-нд ургамал газар зүйн Монгол Алтайн уулын хээрийн тойрогт орших Говь-Алтай аймаг, Хантайширын нуруу, Ялаатын ам (N46о105’, E97о004’), 2019 оны 06-р сарын 23-нд Хянганы уулын нугат хээрийн тойрогт орших Сэлэнгэ аймаг, Мандал сум, Түнхэл тосгоноос (N48o63’, E106o79’) тус тус түүж, шинжилгээнд бэлтгэв. Бидний судалгааны үр дүнд Говь-Алтай ба Сэлэнгэ аймгаас түүж бэлтгэсэн дээжийн нийт чийг 8.25±0.31% ба 8.45±0.13 %, нийт үнс 8.85±0.17 % ба 9.71±0.21 %, сулруулсан хлорт устөрөгчийн хүчилд үл уусах үнс 0.95±0.05 % ба 0.99±0.26 %, усанд хандлагдах бодисын тоо хэмжээ 26.24±0.85 % ба 25.67±0.53 % байв. Энэ ургамлын биологийн идэвхт гол нэгдлүүдийн тооны шинжилгээний дүнд нийт фенолт нэгдэл галлын хүчилд шилжүүлснээр 2.80±0.05 % ба 2.50±0.05 %, нийт флавоноид изоцитизозидод шилжүүлснээр 3.60±0.13 % ба 3.67±0.06 %, нийт идээлэгч бодис пирогаллолд шилжүүлснээр 0.79±0.03 % ба 0.55±0.03 % болохыг тус тус тодорхойлов. Ургамал газар зүйн өөр өөр бүс тойргоос бэлтгэсэн ургамлын - эмийн түүхий эдийн чанарын хяналтын тоон үзүүлэлтүүд ба биологийн идэвхт гол үйлчлэгч бодисуудын тоо хэмжээ ойролцоо дүнтэй байгааг тодорхойлов. Түлхүүр үг: Сибирь удвал, чанарын хяналтын тоон үзүүлэлтүүд, фенолт нэгдэл, флавоноид, идээлэгч бодис  

scholarly journals Quantitative Analysis of Total Flavonoids and Total Phenolic Acids in Thirty Hypericum Taxa

2013 ◽  
Vol 8 (3) ◽  
pp. 1934578X1300800 ◽  
Author(s):  
Kroata Hazler Pilepić ◽  
Željan Maleš ◽  
Maja Crkvenčić
Keyword(s):  
Quantitative Analysis ◽  
Phenolic Acids ◽  
Total Phenolic ◽  
Total Flavonoids ◽  
Spectrophotometric Methods ◽  
Aerial Parts

The content of total flavonoids and total phenolic acids in the aerial parts of thirty Hypericum taxa collected over two consecutive seasons was investigated by spectrophotometric methods. All taxa examined contained flavonoids and phenolic acids in different quantities, although the differences between species and year's harvest were not found to be significant. The quantity of total flavonoids ranged from 0.1 to 1.6%. The highest content of flavonoids was found in the samples of H. japonicum (1.6%) and H. perforatum (1.5%), while the samples of H. androsaemum and H. balearicum comprised the lowest flavonoid amount (0.13%). The content of total phenolic acids in the investigated samples was found to be between 1.1–10.4%. The samples of H. perforatum were found to contain the highest quantity of phenolic acids (10.4% and 10.2%), whereas the sample of H. linarifolium showed the lowest amount of phenolic acids (1.1%).

scholarly journals Сontent of phenolic compounds in the native and alien species of the Bidens genus growing in various regions of Belarus

2020 ◽  
Vol 65 (2) ◽  
pp. 144-152
Author(s):  
T. A. Skuratovich ◽  
O. V. Molchan
Keyword(s):  
Phenolic Compounds ◽  
Alien Species ◽  
Native Species ◽  
Dry Weight ◽  
Total Phenolic ◽  
Total Flavonoids ◽  
Total Phenolic Compounds ◽  
Aerial Parts ◽  
Growing Conditions ◽  
Range Of Variation

The content of phenolic compounds and flavonoids in separate organs and aerial parts of Bidens species growing in different regions of Belarus was studied (alien species – Bidens frondosus L. and Bidens connatus Willd., native species – Bidens tripartitus L.). It was found that the leaves were characterized by the maximum accumulation of total phenolic compounds and total flavonoids. The accumulation of these metabolites decreased in the row of “leaves – inflorescences – stems and roots”. Approximately 14 % of phenolic compounds in leaves and inflorescences were flavonoids. The accumulation of phenolic compounds in the aerial parts of B. frondosus, B. connatus and B. tripartitus, depending on the growing conditions, varied from 60, 55, 90 to 155, 130, 115 mg/g dry weight, flavonoids – from 7, 11, 8 to 20, 15, 19 mg/g dry weight, correspondingly. B. frondosus and B. connatus demonstrated a wider range of variation in the sum of phenolic compounds than B. tripartitus. A comparative analysis of the total phenolic accumulation in plants of different species collected in geographically close localities showed the absence of significant differences in the content of the studied secondary metabolites in alien and native species of Bidens.

Phytochemical composition and antioxidant activity of Malus baccata (L.) Borkh. fruits

2020 ◽  
Vol 3 (1) ◽  
pp. 47
Author(s):  
Nadezhda Petkova ◽  
Manol Ognyanov ◽  
Blaga Inyutin ◽  
Petar Zhelev ◽  
Panteley Denev
Keyword(s):  
Phenolic Compounds ◽  
Antioxidant Activities ◽  
Ethanol Extract ◽  
Titratable Acidity ◽  
Total Phenolic ◽  
Total Phenolic Compounds ◽  
Frap Assay ◽  
Phytochemical Composition ◽  
Malus Baccata

Crab apple (Malus baccata (L.) Borkh.) was mainly distributed in Europe as an ornamental plant, but the nutritional properties of its edible fruits were not fully revealed. The aim of the current study was to characterize the phytochemical composition of ripen carb apple fruits and to evaluate their nutritional and antioxidant potentials. The fruits were assayed for moisture and ash content, proteins, lipids, carbohydrates, titratable acidity (TA), pH, total phenolic compounds and natural pigments. Among the analyzed carbohydrates cellulose was found in the highest content (6% dw), followed by sugars (sucrose, glucose and fructose) and 1.8 % dw uronic acids. The total chlorophylls and carotenoids contents in their fruits were 6.51 and 4.80 μg/g fw, respectively. Total monomeric anthocyanins were not detected. The highest content of total phenolic compounds (2.67 mg GAE/g fw) was found in 95 % ethanol extract from fruits, while the total flavonoids were relatively low – 0.1 mg QE/g fw. DPPH assay (17.27 mM TE/g fw) and FRAP assay (14.34 mM TE/g fw) demonstrated in vitro antioxidant activities of crabapple. Malus baccata fruits were evaluated as a rich source of dietary fibers and phenolic compounds with significant antioxidant potential that could be used in human nutrition.

Chemical Constituents from Chloranthus elatior and Their Inhibitory Effect on Human Dihydroorotate Dehydrogenase

Planta Medica ◽  
2021 ◽  
Author(s):  
Qian Yang ◽  
An Jia ◽  
Xizi Liu ◽  
Shiyi Han ◽  
Siyang Fan
Keyword(s):  
Circular Dichroism ◽  
Chemical Constituents ◽  
Inhibitory Effect ◽  
Mass Spectrometric ◽  
Dihydroorotate Dehydrogenase ◽  
Active Compounds ◽  
Electronic Circular Dichroism ◽  
Aerial Parts ◽  
Ic50 Values ◽  
Circular Dichroïsm

AbstractA new sesquiterpene, chlorantholide G (1), a new sesquiterpene dimer, elatiolactone (2), and 2 new diterpenes, elatiorlabdane B (3) and elatiorlabdane C (4), together with 51 known compounds, were isolated from the aerial parts of Chloranthus elatior. The new structures including their absolute configurations were mainly established by mass spectrometric, NMR, and electronic circular dichroism experiments. All isolated compounds were tested for their anti-hDHODH activity. (4S,6R)-4-hydroxy-6-isopropyl-3-methylcyclohex-2-enone (5) and (4S,5R,9S,10R)-8(17),12,14-labdatrien-18-oic acid (29) were the most active compounds with IC50 values of 18.7 and 30.7 µM, respectively.

scholarly journals Evaluation of Silicon and Proline Application on the Oxidative Machinery in Drought-Stressed Sugar Beet

Antioxidants ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 398
Author(s):  
Muneera D. F. AlKahtani ◽  
Yaser M. Hafez ◽  
Kotb Attia ◽  
Emadeldeen Rashwan ◽  
Latifa Al Husnain ◽  
...  
Keyword(s):  
Antioxidant Enzymes ◽  
Drought Stress ◽  
Phenolic Compounds ◽  
Sugar Beet ◽  
Harmful Effect ◽  
Sugar Yield ◽  
Total Phenolic ◽  
Total Phenolic Compounds ◽  
Sod Activity ◽  
Yield Parameters

Drought stress deleteriously affects growth, development and productivity in plants. So, we examined the silicon effect (2 mmol) and proline (10 mmol) individually or the combination (Si + proline) in alleviating the harmful effect of drought on total phenolic compounds, reactive oxygen species (ROS), chlorophyll concentration and antioxidant enzymes as well as yield parameters of drought-stressed sugar beet plants during 2018/2019 and 2019/2020 seasons. Our findings indicated that the root diameter and length (cm), root and shoot fresh weights (g plant−1) as well as root and sugar yield significantly decreased in sugar beet plants under drought. Relative water content (RWC), nitrogen (N), phosphorus (P) and potassium (K) contents and chlorophyll (Chl) concentration considerably reduced in stressed sugar beet plants that compared with control in both seasons. Nonetheless, lipid peroxidation (MDA), electrolyte leakage (EL), hydrogen peroxide (H2O2) and superoxide (O2●−) considerably elevated as signals of drought. Drought-stressed sugar beet plants showed an increase in proline accumulation, total phenolic compounds and up-regulation of antioxidant enzymes catalase (CAT) and superoxide dismutase (SOD) activity to mitigate drought effects. Si and proline individually or the combination Si + proline considerably increased root and sugar yield, sucrose%, Chl concentration and RWC, MDA and EL were remarkably reduced. The treatments led to adjust proline and total phenolic compounds as well as CAT and SOD activity in stressed sugar beet plants. We concluded that application of Si + proline under drought stress led to improve the resistance of sugar beet by regulating of proline, antioxidant enzymes, phenolic compounds and improving RWC, Chl concentration and Nitrogen, Phosphorus and Potassium (NPK) contents as well as yield parameters.

Vegetative propagation, chemical composition and antioxidant activity of yerba mate genotypes

2021 ◽  
pp. 1-10
Author(s):  
Leandro Marcolino Vieira ◽  
Renata de Almeida Maggioni ◽  
Jéssica de Cássia Tomasi ◽  
Erik Nunes Gomes ◽  
Ivar Wendling ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Vegetative Propagation ◽  
Adventitious Rooting ◽  
Ilex Paraguariensis ◽  
Total Phenolic ◽  
Yerba Mate ◽  
Total Phenolic Compounds ◽  
Leaf Retention ◽  
Correlated Components

Abstract Ilex paraguariensis, commonly known as yerba mate, is a tree species native to South America. Its commercial value is due to the manufacturing of teas, with potential also in the pharmacological and cosmetic industries. Vegetative propagation of yerba mate is considered an innovation to the traditional production systems based on sexual propagation. The present study aimed to evaluate the rhizogenic potential and chemical attributes of mini-cuttings from 15 yerba mate genotypes, as well as to verify the correlation between phytochemical and rooting-related variables. Mini-cuttings were collected from a pre-existing mini-clonal hedge and the experimental design was completely randomized, with 15 treatments (genotypes), four replications and 10 mini-cuttings per plot. After 120 days, mini-cuttings were assessed regarding rooting, mortality, callogenesis and leaf retention percentages, percentage of mini-cuttings with both calluses and roots, number of roots and average root length. At the time of collection, subsamples from each plot were used for phytochemical analyses including total phenolic compounds, protein, caffeine and theobromine contents and antioxidant activity. Rooting percentages ranged from 5 to 72.5%, with significant variation among genotypes. Adventitious rooting and phytochemical profile of yerba mate mini-cuttings are genotype-dependent. Leaf retention is a relevant factor in the rooting of yerba mate mini-cuttings and the levels of total phenolic compounds, antioxidants and theobromine present in mini-cuttings are negatively correlated components to Ilex paraguariensis adventitious rooting.

scholarly journals Impact of Specialty Malts on Wort and Beer Characteristics

Fermentation ◽  
2021 ◽  
Vol 7 (3) ◽  
pp. 137
Author(s):  
Luis F. Castro ◽  
Abigail D. Affonso ◽  
Renata M. Lehman
Keyword(s):  
Phenolic Compounds ◽  
Free Amino ◽  
Amino Nitrogen ◽  
The Other ◽  
Fermentable Sugars ◽  
Total Phenolic ◽  
Total Phenolic Compounds ◽  
Polyphenol Content ◽  
The Impact

Specialty malts are commonly used in brewing to provide flavor, aroma, and color to wort and beer. The use of specialty malts contributes to the variety of beer products; therefore, it is important to understand their effect on the characteristics of wort and beer. This study investigates the impact of various specialty malts on wort and beer properties. A control beer was prepared with 100% base malt, and four beer treatments were prepared with the addition of kilned, roasted, and caramel specialty malts. For each treatment, 20% of the base malt was substituted with the various specialty malts when preparing the wort. The fermentable sugars and free amino nitrogen (FAN) content for each wort were analyzed. Alcohol by volume (ABV), international bitterness units (IBU), diacetyl, and polyphenol content of each prepared beers were subsequently analyzed. Results showed that wort prepared with the addition of roasted and caramel malts contained a lower concentration of fermentable sugars and FAN than wort prepared with the base and kilned malts. Beers prepared with the addition of roasted and caramel malts exhibited the lowest levels of ABV, as well as the lowest levels of diacetyl. These beers also exhibited higher levels of total phenolic compounds compared to the other beer samples. No change was observed in IBU levels as a result of brewing with the different specialty malts. This study illustrates how the use of specialty malts impacts wort and beer properties, providing useful information to aid in the production of quality beer products.

scholarly journals Bioconversion of Flavonoid Glycosides from Hippophae rhamnoides Leaves into Flavonoid Aglycones by Eurotium amstelodami

2019 ◽  
Vol 7 (5) ◽  
pp. 122 ◽  
Author(s):  
Qiuya Gu ◽  
Guoliang Duan ◽  
Xiaobin Yu
Keyword(s):  
Antioxidative Activity ◽  
Hippophae Rhamnoides ◽  
Flavonoid Glycosides ◽  
Total Phenolic ◽  
Total Phenolic Compounds ◽  
Flavonoid Aglycones ◽  
Hippophaë Rhamnoides ◽  
Tea Industry ◽  
Functional Factors ◽  
Flowering Process

The flowering process has been reported to play crucial roles in improving the flavor and efficacy of fermented tea. Hippophae rhamnoides leaves containing many beneficial ingredients are a suitable plant source for tea processing. In this study, we isolated a β-glucosidase-producing fungus Eurotium amstelodami BSX001 from the fermented tea and used Hippophae rhamnoides leaves (HRL) as a substrate to explore the detailed process of bioconversion of some important functional factors. The results show that the contents of total phenolic compounds and flavonoids increased significantly after seven days, especially flavonoid aglycones (e.g., quercetin, kaempferol, and isorhamnetin). Such compounds greatly enhance the antioxidative activity of fermented products. Metabolic analysis of the standard compounds (rutin, quercetin-3-glucoside, kaempferol-3-glucoside, quercetin, isorhamnetin-3-glucoside, isorhamnetin, and kaempferol) further confirmed the effective biotransformation by E. amstelodami. Mechanisms of the bioconversion could be involved in deglycosylation, dihydroxylation, and O-methylation. Our findings expand the understanding of tea fermentation process and provide further guidance for the fermented tea industry.

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