scholarly journals Changes in Oxidative Stress Index and Lipid Peroxidation Product in the Brain of Rats with Lesion of Central Dopaminergic System after Propofol Administration*

2019 ◽  
Vol 73 ◽  
pp. 337-343
Author(s):  
Ewa Romuk ◽  
Wioletta Szczurek ◽  
Przemysław Nowak ◽  
Magdalena Prudel-Babiuch ◽  
Ryszard Szkilnik ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Parkinson’S Disease ◽  
Lipid Peroxidation ◽  
Parkinson's Disease ◽  
Stress Index ◽  
Control Group ◽  
Lipid Peroxidation Product ◽  
Oxidative Stress Index ◽  
Group I ◽  
Group Ii

Propofol is a commonly used intravenous anesthetic agent with antioxidant properties. However, the effect of propofol on oxidative stress index (OSI) and lipid peroxidation in Parkinson’s disease is still unknown. The present study aimed to evaluate the effect of propofol on OSI and malondialdehyde (MDA) level in the selected brain regions of the rats with Parkinson’s disease (PD). 32 male Wistar rats were divided into four groups: I- control group, II- group with PD, III-control group with propofol, IV-PD group with propofol. 60mg/kg of propofol was given to the 8-weeks-old rats intraperitoneally, and the selected parts of the rats’ brains (frontal cortex, striatum, thalamus and hippocampus) were isolated after decapitation. The concentration of MDA, which is a marker of lipid peroxidation, and OSI were measured. In group IV compared to group II, was observed a significant MDA level decrease in the cortex (39%, p <0.001), striatum (28%, p <0.001), hippocampus (21%, p <0.05) and thalamus (20%, p <0.05), together with a decreased OSI level in the thalamus (71%, p <0,001), cortex (70%, p <0.05), striatum (65%, p <0.001), and hippocampus (57%, p <0.05). In group III compared to group I was observed decrease in MDA level in the cortex (40%, p <0.001). Propofol inhibits oxidative stress in all the evaluated structures of the rat brain with Parkinson’s disease. There are significant differences in the response of brain tissues to administered propofol between rats with PD and healthy ones.

scholarly journals Total Antioxidant Capacity and Lipid Peroxidation Status in Cervical Cancer Patients Compared with Women Without Cervical Cancer in Bangladesh

2021 ◽  
Vol 19 (4) ◽  
Author(s):  
Taslima Nigar ◽  
Annekathryn Goodman ◽  
Shahana Pervin
Keyword(s):  
Oxidative Stress ◽  
Cervical Cancer ◽  
Lipid Peroxidation ◽  
Ascorbic Acid ◽  
Antioxidant Capacity ◽  
Cancer Patients ◽  
Total Antioxidant Capacity ◽  
Stress Index ◽  
Oxidative Stress Index ◽  
Total Antioxidant

Abstract Purpose Over the past several decades, research has suggested reactive oxygen species act as cofactors for cervical cancer development. The aim of this study is to evaluate the antioxidant and lipid peroxidation status in cervical cancer patients in Bangladesh. Methods From December 2017 to 2018, a cross-sectional observational study was conducted on 50 cervical cancer patients and 50 controls. Plasma levels of lipid peroxidation and total antioxidant capacity were measured. The Student’s t test was used for statistical analysis. P values less than 0.05 were taken as a level of significance. Results There was a significant reduction in total antioxidant levels in patients with cervical cancer, 972.77 ± 244.22 SD µmol equivalent to ascorbic acid/L, compared to normal controls, 1720.13 ± 150.81 SD µmol equivalent to ascorbic acid/L (P < 0.001). Levels of lipid peroxidation were found to be significantly higher in cervical cancer, 7.49 ± 2.13 SD µmol/L, than in women without cervical cancer, 3.28 ± 0.58 SD µmol/L (P < 0.001). The cervical cancer patients had significantly higher levels of oxidative stress index (0.83 ± 0.31) in comparison to controls (0.19 ± 0.04) (P < 0.001). Conclusion There was an increased oxidative stress index due to imbalance between lipid peroxidation generation and total antioxidant capacity in cervical cancer patients. Further studies are needed to explore the role of oxidative stress as a cofactor for cervical carcinogenesis.

Evaluation of Dynamic Thiol-Disulfide Balance in Preinvasive Lesions of the Cervix

2021 ◽  
Author(s):  
Burak Sezgin ◽  
Fatih Pirinççi ◽  
Aysun Camuzcuoğlu ◽  
Eda Adeviye Şahin ◽  
Özcan Erel ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Human Papillomavirus ◽  
Stress Index ◽  
Lesion Group ◽  
Control Group ◽  
High Grade ◽  
Squamous Intraepithelial Lesion ◽  
Oxidative Stress Index ◽  
Preinvasive Lesions ◽  
Intraepithelial Lesion

Abstract Purpose: This study aimed to determine the potential clinical use of dynamic thiol disulfide balance in cases with preinvasive lesions of the cervix.Methods: One hundred and sixteen patients with high-grade squamous intraepithelial lesion, one hundred patients with low-grade squamous intraepithelial lesion and one hundred and ten healthy controls were enrolled in the study. A fully automated colorimetric system was used to determine the levels of thiol-disulfide parameters. The ischemia-modified albumin, total oxidant-antioxidant capacity, oxidative stress index of the retrieved cases were further analysed.Results: Native thiol and total thiol levels are significantly lower in the high-grade squamous intraepithelial lesion group according to control group (p:0.004 and p:0.015, respectively). Disulfide level is significantly increased in the high-grade squamous intraepithelial lesion group compared to control group (p:0.004). Oxidative stress index levels in high-grade squamous intraepithelial lesion group were observed as significantly higher according to the control group (p:0.014). Ischemia-modified albumin levels in the high-grade squamous intraepithelial lesion group were observed as significantly higher compared to the control group (p:0.020). Disulfide levels are positively correlated with risk type of Human papillomavirus (r:0.420, p<0.001).Conclusion: The analysis of dynamic thiol disulfide balance revealed considerable oxidative damage in patients with Human papillomavirus -related cervical precursor lesions compared to women with ordinary cytology specimens. Therefore, investigation of thiol disulfide balance with presented method represents a new promising test for early diagnosis and management of women at high risk for cervical cancer.

scholarly journals The Possible Role of Bifidobacterium longum BB536 and Lactobacillus rhamnosus HN001 on Locomotor Activity and Oxidative Stress in a Rotenone-Induced Zebrafish Model of Parkinson’s Disease

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Ovidiu-Dumitru Ilie ◽  
Emanuela Paduraru ◽  
Madalina-Andreea Robea ◽  
Ioana-Miruna Balmus ◽  
Roxana Jijie ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Locomotor Activity ◽  
Prolonged Exposure ◽  
Bifidobacterium Longum ◽  
The Body ◽  
The Other ◽  
Control Group

Background. As every organ within the body, the brain is also extremely susceptible to a plethora of noxious agents that change its chemistry. One component frequently found in current products against harmful species to crops is rotenone whose effect under prolonged exposure has been demonstrated to cause neurodegenerative disorders such as Parkinson’s disease. The latest reports have indeed revealed that rotenone promotes Parkinson’s in humans, but studies aiming to show congruent effects in zebrafish (Danio rerio) are lacking. Material and Methods. In this context, the aim of the present study was to demonstrate how chronic administration of rotenone for 3 weeks impairs the locomotor activity and sociability and induces oxidative stress in zebrafish. Results. There were no statistically significant differences following the analysis of their social interaction and locomotor tests ( p > 0.05 ). However, several exceptions have been noted in the control, rotenone, and probiotics groups when we compared their locomotor activity during the pretreatment and treatment interval ( p < 0.05 ). We further assessed the role of rotenone in disturbing the detoxifying system as represented by three enzymes known as superoxide dismutase (SOD), glutathione peroxidase (GPx), and malondialdehyde (MDA). Despite the fact that there were no statistically significant changes within SOD and GPx levels between the control group and rotenone, probiotics, and rotenone + probiotics ( p > 0.05 ), relevant changes have been observed between the analyzed groups ( p < 0.05 and p < 0.005 , respectively). On the other hand, significant differences ( p < 0.05 ) have been observed for MDA when we analyzed the data between the control group and the other three groups. Conclusions. Our results suggest that rotenone can be successfully used to trigger Parkinson’s disease-related symptomatology in zebrafish.

scholarly journals Effects of Flunixin Meglumine on Oxidant and Antioxidant System after Disbudding with Caustic Paste in Calves

2018 ◽  
Vol 46 (1) ◽  
pp. 9
Author(s):  
Selvinaz Yakan ◽  
Vesile Duzguner ◽  
Ozgur Aksoy
Keyword(s):  
Oxidative Stress ◽  
Local Anesthesia ◽  
Antioxidant System ◽  
Live Weight ◽  
Stress Index ◽  
Blood Samples ◽  
Flunixin Meglumine ◽  
Group I ◽  
Group Ii ◽  
Commercial Kit

Background: Disbudding is often practiced in modern farm because of the reasons for the safekeeping of the animal, other animals lessen the risk of injury and less aggressive behavior. Without regard to the method of disbudding leads to disruption behavioral changes, cardiac and endocrine responses related pain of animals. Sedation, local anesthesia and analgesia are performed studies in order to eliminate of cardiac, endocrine, behavioral response which is caused by pain associated with disbudding. In this study, it was aimed to determine the effect on the oxidant and antioxidant system in the calves of disbudding by using the caustic paste with and without painkiller.Materials, Methods & Results: The animal material of the study was created 24 Simmentals calves in different sexes on average 2 weeks (± 2 days) and 50 kg (± 15 kg) live weight. The cases were randomly divided into 2 groups of 12 calves in each group. In the group I (analgesia group); caustic paste+painkiller (2.2 mg-1 flunixin meglumine intravenous, IV) and group II (non-analgesia group); as disbudding applied with caustic pasta application only were divided into 2 groups. In the I group, 15th min before the application, flunixin meglumine at a dose of 2.2 mg-1 was administered IV and the horn blunting was performed by caustic paste method. Blood samples were taken at 15, 30 and 60th min after completion of horn blunting and physiological findings were recorded. In group II, the horns of the calves were disbudding with the same technique (but no analgesia aplication). Blood samples were taken at 15, 30 and 60th min after completion of horn blunting and physiological findings were record. Cortisol, glucose, total oxidant capacity (TOC) and total antioxidant capacity (TAC), 8-hydroxy-2’-deoxyguanosine (8-OHdG), glutathione (GSH), superoxide dismutase (SOD) enzymes measurements (ELISA) were performed in both groups. TOC and TAC tests were used to determine the oxidative stress index in the obtained serum, 8-OHdG measurement which is the most sensitive and most frequent oxidative DNA damage indicator for determination of oxidative damage; GSH, SOD enzymes were analyzed by ELISA using commercial kit for the evaluation of antioxidant potential. For evaluation of pain in animals, cortisol (ELISA) and glucose level in serum were measured with ELISA by using commercial kit. In the group without flunixin meglumine, cortisol was increased significantly from the 15th min (P < 0.05), but there was no increase in the 30 and 60th min in painkiller administered group. Glucose levels were higher in the group not using painkillers than those used at 15th (P < 0.001) and 30, 60th min (P < 0.05). TOC (P < 0.001) and 8-OHdG (P < 0.05) values were significantly higher in the group without painkiller while TAC, GSH and SOD levels (P < 0.05) were significantly decreased.Discussion: It has been observed that sedation (such as xylazine) and local anesthesia (such as lidocaine) use removed pain-induced stress in the disbudding with caustic paste in calves. However, in order to remove the endocrine response, it is necessary to combine the anesthesia protocol with the analgesic (such as flunixin meglumine) combination. According to our results concluded that the painkiller given before the disbudding process was supporting the antioxidant system, reducing the level of cortisol and oxidative stress.

scholarly journals An Enriched Environment Ameliorates Oxidative Stress and Olfactory Dysfunction in Parkinson’s Disease with α-Synucleinopathy

2018 ◽  
Vol 27 (5) ◽  
pp. 831-839 ◽  
Author(s):  
Soohyun Wi ◽  
Jang Woo Lee ◽  
MinGi Kim ◽  
Chang-Hwan Park ◽  
Sung-Rae Cho
Keyword(s):  
Oxidative Stress ◽  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Underlying Mechanism ◽  
Olfactory Dysfunction ◽  
Olfactory Function ◽  
Enriched Environment ◽  
Detoxification Enzymes ◽  
Paraoxonase 1 ◽  
Control Group

Parkinson’s disease (PD) features nonmotor symptoms such as olfactory dysfunction referred to as hyposmia, an initial sign of disease progression. Metabolic dysfunction can contribute to neurodegenerative diseases, and various xenobiotics and endogenous compounds are also involved in the pathogenesis of PD. Although aerobic exercise was found to induce preservation or improvement in olfactory function in PD patients in a recent study, the exact underlying mechanism for this effect is not clear. We aimed to investigate the influence of an enriched environment (EE) on olfactory dysfunction especially via metabolic pathways related to detoxification enzymes. Eight-month-old transgenic (Tg) PD mice that overexpress human A53T α-synuclein (α-syn) were randomly allocated to an EE or standard conditions for 2 mo. The buried food test showed that EE group had significantly improved olfactory function compared to the control group. Reverse transcription polymerase chain reaction (PCR) and real-time quantitative PCR showed that expression of the detoxification enzymes–– cytochrome P450 family 1 subfamily A member 2, paraoxonase 1, alcohol dehydrogenase 1, UDP glucuronosyltransferase family 2 member A1 complex locus, aldehyde oxidase homolog 2, and aldehyde glutathione peroxidase 6––was significantly increased in the olfactory bulb (OB) of the PD control group, but these enzymes were normalized in the EE group. Immunohistochemical staining of the OB showed that oxidative stress and nitrated α-syn were significantly increased in the control group but decreased in the EE group. In conclusion, we suggest that exposure to an EE decreases both oxidative stress and nitrated α-syn, resulting in normalized detoxification enzymes and amelioration of olfactory dysfunction.

scholarly journals Methyl Gallate Attenuates Doxorubicin-Induced Cardiotoxicity in Rats by Suppressing Oxidative Stress

Scientifica ◽  
2021 ◽  
Vol 2021 ◽  
pp. 1-12
Author(s):  
Akheruz Zaman Ahmed ◽  
Shakta Mani Satyam ◽  
Prakashchandra Shetty ◽  
Melanie Rose D’Souza
Keyword(s):  
Oxidative Stress ◽  
Normal Control ◽  
Wistar Rats ◽  
Group Iv ◽  
Control Group ◽  
Methyl Gallate ◽  
Group Iii ◽  
Group I ◽  
Female Wistar Rats ◽  
Group Ii

Doxorubicin-induced cardiotoxicity is the leading cause of morbidity and mortality among cancer survivors. The present study was aimed to investigate the cardioprotective potential of methyl gallate; an active polyphenolic nutraceutical, against doxorubicin-induced cardiotoxicity in Wistar rats. Twenty-four female Wistar rats (150–200 g) were divided into four groups (n = 6) which consist of normal control (group I), doxorubicin control (group II), test-A (group III), and test-B (group IV). Group III and group IV animals were prophylactically treated with methyl gallate 150 mg/kg/day and 300 mg/kg/day orally, respectively, for seven days. Doxorubicin (25 mg/kg; single dose) was administered through an intraperitoneal route to group II, III, and IV animals on the seventh day to induce acute cardiotoxicity. On the 8th day, besides ECG analysis, serum CK, CK-MB, LDH, AST, MDA, and GSH were assayed. Following gross examination of isolated hearts, histopathological evaluation was performed by light microscopy. A significant ( p  < 0.05) cardiac injury, as well as oxidative stress, was observed in doxorubicin control rats in comparison to normal control rats. Methyl gallate at both the doses significantly ( p  < 0.05) reduced doxorubicin-induced ECG changes, dyslipidaemia, and elevation of CK, CK-MB, LDH, AST, MDA and increased GSH level. Methyl gallate reversed the doxorubicin-induced histopathological changes in the heart. The present study revealed that methyl gallate exerts cardioprotection against doxorubicin-induced cardiotoxicity in female Wistar rats by suppressing oxidative stress. Our study opens the perspective to clinical studies for consideration of methyl gallate as a potential chemoprotectant nutraceutical in the combination chemotherapy with doxorubicin to limit its cardiotoxicity.

scholarly journals Effects of Metformin in Combination with a Herbal Capsule (Glucoblock) on Insulin Resistance and Oxidative Stress Index in Type 2 Diabetic Rats

2021 ◽  
pp. 18-25
Author(s):  
Ojoye N. Briggs ◽  
Kemzi N. Elechi-amadi ◽  
Justice C. Ohaka ◽  
Edna O. Nwachuku ◽  
Bartimaeus S. Ebirien-agana
Keyword(s):  
Oxidative Stress ◽  
Insulin Resistance ◽  
Diabetic Control ◽  
Negative Control ◽  
Diabetic Rats ◽  
Stress Index ◽  
Control Group ◽  
Oxidative Stress Index ◽  
Treatment Groups ◽  
And Oxidative Stress

Aim: This study evaluated the effects of metformin in combination with a herbal capsule (glucoblock) on insulin resistance and oxidative stress index in type 2 diabetic rats. Methodology: A total of 35 male Wistar albino rats weighing between 120-220 g were used for this study. The rats were placed on high fat diet, and diabetes was induced by a single intraperitoneal injection of freshly prepared streptozotocin (STZ) (45 mg/kg body wt). Fasting plasma glucose (FPG) was determined using the glucose oxidase method. Fasting plasma insulin (FPI), total oxidant status (TOS), total antioxidant status (TAS) and superoxide dismutase (SOD) levels were quantitatively determined by a rat-specific sandwich-enzyme linked immunosorbent assay (ELISA) method. Insulin resistance (IR) was determined using the homeostatic model assessment for insulin resistance (HOMA-IR) method. Oxidative stress index (OSI) was determined by the ratio of TOS to TAS. Phytochemical analysis on the herbal capsule was done using classical methods. Results: The results revealed the presence of alkaloids (100.31μg/mg), flavonoids (131.45μg/mg), cardiac glycosides (55.93μg/mg) and saponins (61.47μg/mg) in the herbal drug glucoblock. The results showed significantly lower FPG levels in the treatment groups when compared to the diabetic control. Group 3 administered metformin had significantly higher FPG levels compared to the negative control. Group 4 administered the herbal drug glucoblock and group 5 administered a combination of metformin and glucoblock, showed no significant differences in FPG levels when compared to the negative control. The diabetic control had significantly higher FPI levels compared to the negative control and treatment groups. The treatment groups showed no significant differences in FPI levels when compared to the negative control. HOMA-IR was significantly higher in the diabetic control compared to the negative control and treatment groups. Also, HOMA-IR values in the treatment groups showed no significant difference compared to the negative control except for group 3 (metformin), that was significantly higher than the negative control. SOD was significantly lower in the diabetic control, compared to the negative control and treatment groups. There were no significant differences in SOD levels in the treatment groups compared to the negative control. TOS levels in the negative control group and treatment groups were significantly lower, compared to the diabetic control. TAS was significantly lower in the diabetic control and treatment groups compared to the negative control. OSI in the diabetic control was significantly higher, compared to the negative control and treatment groups. Also, the treatment groups had significantly higher OSI compared to the negative control. Conclusion: High fat diet and streptozotocin induction produced significant insulin resistance and oxidative stress in the diabetic rats. Glucoblock was more effective in reducing insulin resistance compared to metformin. The combination showed synergistic drug-herb reaction as glucoblock potentiated the actions of metformin. Both showed antioxidant potential but were not effective in lowering oxidative stress to normal levels. There is need to incorporate antioxidant therapy in the treatment protocol for diabetes mellitus.

scholarly journals Effect of Curcumin on Oxidative Stress in a Model of Turpentine Induced Acute Experimental Inflammation

2017 ◽  
Vol 45 (1) ◽  
pp. 65-74 ◽  
Author(s):  
Andrei CONEAC ◽  
Meda Sandra ORASAN ◽  
Daniel Corneliu LEUCUTA ◽  
Nicoleta DECEA ◽  
Miuta FILIP ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
High Performance ◽  
Control Group ◽  
Group Iii ◽  
Group I ◽  
Total Antioxidant ◽  
Experimental Inflammation ◽  
Group Ii

Curcumin, a natural phenolic compound is an anti-tumor agent with anti-inflammatory and anti-oxidant properties. The aim of this research was to evaluate oxidative stress levels, the antioxidant activity and Curcumin concentrations by high performance liquid chromatography (HPLC) in an acute experimental inflammation induced by Turpentine oil (intramuscular 0.6 mg kg-1 body weight) and to compare a prophylactic versus a therapeutic regimen of Curcumin (oral suspension of 150 mg Curcumin kg-1 rat weight). Sixteen adult male Wistar rats were assigned to four groups: Control, Group I (Curcumin only), Group II (Curcumin administration, then induced inflammation after 1 hour) and Group III (induced inflammation then Curcumin administration after 2 hours). Oxidative stress was assessed by measuring serum malondialdehide and carbonylated proteins, while systemic and local total antioxidant capacity was determined by ABTS. Local tissue changes (muscle, kidney, liver) were analysed using histopathology. Results showed that acute inflammation significantly increased lipid peroxidation in Groups II and III compared to Control and Group I. A significantly reduced total antioxidant capacity (ATBS) was present in serum and kidney in Group II, also in muscle and kidney in Group III. ABTS levels were significantly increased only in the liver tissue of the animals in Groups II and III with induced inflammation as compared to Group I. This study proved the potential of Curcumin in reducing oxidative stress in both prophylactic and therapeutic regimens.

scholarly journals Effects of carvacrol and physical exercise on motor and memory impairments associated with Parkinson’s disease

2019 ◽  
Vol 77 (7) ◽  
pp. 493-500 ◽  
Author(s):  
Leila HAMZEHLOEI ◽  
Mohammad Ebrahim REZVANI ◽  
Ziba RAJAEI
Keyword(s):  
Oxidative Stress ◽  
Parkinson’S Disease ◽  
Lipid Peroxidation ◽  
Parkinson's Disease ◽  
Treadmill Exercise ◽  
Memory Deficit ◽  
Rotational Behavior ◽  
Oxidative Stress Biomarkers ◽  
Stress Biomarkers ◽  
And Oxidative Stress

ABSTRACT The present study was undertaken to investigate the effects of carvacrol and treadmill exercise on memory deficit, rotational behavior and oxidative stress biomarkers in a 6-OHDA-lesioned rat model of Parkinson’s disease. Wistar rats were treated with carvacrol at a dose of 25 mg/kg and/or ran on a treadmill for a week. Then, 6-OHDA was microinjected into the medial forebrain bundle and treatments continued for six more weeks. Aversive memory, rotational behavior and oxidative stress biomarkers were assessed at the end of week six. The 6-OHDA-lesioned group showed a significant increase in rotational behavior and a decrease in step-through latency in the passive avoidance test compared with the sham group. These behaviors were accompanied by increased lipid peroxidation levels and decreased total thiol concentration in the striatum and/or hippocampus of the hemiparkinsonian rats. Moreover, treatment with carvacrol and exercise reduced rotational behavior and improved aversive memory deficit, which was accompanied by decreased lipid peroxidation levels and increased total thiol concentration in the striatum and/or hippocampus. In conclusion, treatment with carvacrol and treadmill exercise ameliorated motor and memory deficits by modulating oxidative stress in the striatum and hippocampus of hemiparkinsonian rats. Therefore, the combination of carvacrol and treadmill exercise could be an effective therapeutic tool for treatment of neurobehavioral deficits in Parkinson’s disease patients.

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