scholarly journals Salinity Tolerance in Canola: Insights from Proteomic Studies

2021 ◽  
Author(s):  
Ali Bandehagh ◽  
Zahra Dehghanian ◽  
Robert Henry ◽  
Mohammad Anwar Hossain
Keyword(s):  
Edible Oils ◽  
Biodiesel Fuel ◽  
Brassica Napus L ◽  
Crop Species ◽  
Worldwide Production ◽  
The Family ◽  
Salinity Levels ◽  
Plant Characteristics ◽  
Brassicaceae Family ◽  
Adaptation To Salinity

Salinity considerably lowers crop yield worldwide. Production of salt stress-tolerant species will be essential to maintain the food supply in the coming decades. Brassicas, including various members of the family Brassicaceae, are very necessary sources of human food. Importantly, the key crop species that are members of the Brassicaceae family are genetically diverse and therefore their response reaction and adaptation to salinity varies greatly. Canola (Brassica napus L.) is commonly grown for edible oils and other uses such as biodiesel fuel production. Although most types of canola are identified as salt-resistant, plant yield and development are reduced significantly by rising salinity levels. In saline situations, the plant’s genome supports a range of physiological changes in some plant characteristics. Since the function of genes cannot indicate the exact condition of cells, proteomic approaches are emerged as methods to investigate the plant’s responses to stresses in the molecular levels. Exploring the proteome complements research at the genome and transcriptome level and helps elucidate the mechanism of salt tolerance in plants. Proteins are reliable indicators of salinity responses, as they are directly involved in forming the new phenotype providing adaptation to salinity. In this chapter, we review the response of the rapeseed proteome to salinity stress.

scholarly journals Molecular response of canola to salt stress: insights on tolerance mechanisms

2018 ◽  
Author(s):  
Reza Shokri-Gharelo ◽  
Pouya Motie-Noparvar
Keyword(s):  
Salt Stress ◽  
Salt Tolerance ◽  
Edible Oils ◽  
Biodiesel Fuel ◽  
Molecular Response ◽  
Salt Tolerant ◽  
Tolerance Mechanisms ◽  
Brassica Napus L ◽  
Salt Response ◽  
The Many

Canola (Brassica napus L.) is widely cultivated around the world for the production of edible oils and biodiesel fuel. Despite many canola varieties being described as ‘salt-tolerant’, plant yield and growth decline drastically with increasing salinity. Although many studies have resulted in better understanding of the many important salt-response mechanisms that control salt signaling in plants, detoxification of ions, and synthesis of protective metabolites, the engineering of salt-tolerant crops has only progressed slowly. Genetic engineering has been considered as an efficient method for improving the salt tolerance of canola but there are many unknown or little-known aspects regarding canola response to salinity stress at the cellular and molecular level. In order to develop highly salt-tolerant canola, it is essential to improve knowledge of the salt-tolerance mechanisms, especially the key components of the plant salt-response network. In this review, we focus on studies of the molecular response of canola to salinity to unravel the different pieces of the salt response puzzle. The paper includes a comprehensive review of the latest studies, particularly of proteomic and transcriptomic analysis, including the most recently identified canola tolerance components under salt stress, and suggests where researchers should focus future studies.

scholarly journals Molecular response of canola to salt stress: insights on tolerance mechanisms

2018 ◽  
Author(s):  
Reza Shokri-Gharelo ◽  
Pouya Motie-Noparvar
Keyword(s):  
Salt Stress ◽  
Salt Tolerance ◽  
Edible Oils ◽  
Biodiesel Fuel ◽  
Molecular Response ◽  
Salt Tolerant ◽  
Tolerance Mechanisms ◽  
Brassica Napus L ◽  
Salt Response ◽  
The Many

Canola (Brassica napus L.) is widely cultivated around the world for the production of edible oils and biodiesel fuel. Despite many canola varieties being described as ‘salt-tolerant’, plant yield and growth decline drastically with increasing salinity. Although many studies have resulted in better understanding of the many important salt-response mechanisms that control salt signaling in plants, detoxification of ions, and synthesis of protective metabolites, the engineering of salt-tolerant crops has only progressed slowly. Genetic engineering has been considered as an efficient method for improving the salt tolerance of canola but there are many unknown or little-known aspects regarding canola response to salinity stress at the cellular and molecular level. In order to develop highly salt-tolerant canola, it is essential to improve knowledge of the salt-tolerance mechanisms, especially the key components of the plant salt-response network. In this review, we focus on studies of the molecular response of canola to salinity to unravel the different pieces of the salt response puzzle. The paper includes a comprehensive review of the latest studies, particularly of proteomic and transcriptomic analysis, including the most recently identified canola tolerance components under salt stress, and suggests where researchers should focus future studies.

scholarly journals Molecular response of canola to salt stress: insights on tolerance mechanisms

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e4822 ◽  
Author(s):  
Reza Shokri-Gharelo ◽  
Pouya Motie Noparvar
Keyword(s):  
Salt Stress ◽  
Salt Tolerance ◽  
Edible Oils ◽  
Biodiesel Fuel ◽  
Molecular Response ◽  
Salt Tolerant ◽  
Tolerance Mechanisms ◽  
Brassica Napus L ◽  
Salt Response ◽  
The Many

Canola (Brassica napus L.) is widely cultivated around the world for the production of edible oils and biodiesel fuel. Despite many canola varieties being described as ‘salt-tolerant’, plant yield and growth decline drastically with increasing salinity. Although many studies have resulted in better understanding of the many important salt-response mechanisms that control salt signaling in plants, detoxification of ions, and synthesis of protective metabolites, the engineering of salt-tolerant crops has only progressed slowly. Genetic engineering has been considered as an efficient method for improving the salt tolerance of canola but there are many unknown or little-known aspects regarding canola response to salinity stress at the cellular and molecular level. In order to develop highly salt-tolerant canola, it is essential to improve knowledge of the salt-tolerance mechanisms, especially the key components of the plant salt-response network. In this review, we focus on studies of the molecular response of canola to salinity to unravel the different pieces of the salt response puzzle. The paper includes a comprehensive review of the latest studies, particularly of proteomic and transcriptomic analysis, including the most recently identified canola tolerance components under salt stress, and suggests what researchers should focus on in future studies.

The extensin gene family in oilseed rape (Brassica napus L.): Characterisation of sequences of representative members of the family

1990 ◽  
Vol 223 (2) ◽  
pp. 273-287 ◽  
Author(s):  
I. Marta Evans ◽  
Laurence N. Gatehouse ◽  
John A. Gatehouse ◽  
Jennifer N. Yarwood ◽  
Donald Boulter ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Gene Family ◽  
Oilseed Rape ◽  
Brassica Napus L ◽  
The Family

scholarly journals Genetic Diversity in Commercial Rapeseed (Brassica napus L.) Varieties from Turkey as Revealed by RAPD

2013 ◽  
Vol 5 (1) ◽  
pp. 114-119 ◽  
Author(s):  
Özlem ÖZBEK ◽  
Betül Uçar GIDIK
Keyword(s):  
Genetic Diversity ◽  
Brassica Napus ◽  
Dna Analysis ◽  
Diversity Index ◽  
Principal Component ◽  
Brassica Napus L ◽  
Binary Matrix ◽  
Crop Species ◽  
Commercial Crop ◽  
The Mean

In cultivated commercial crop species, genetic diversity tends to decrease because of the extensive breeding processes. Therefore, germplasm of commercial crop species, such as Brassica napus L. should be evaluated and the genotypes, which have higher genetic diversity index, should be addressed as potential parental cross materials in breeding programs. In this study, the genetic diversity was analysed by using randomly amplified polymorphic DNA analysis (RAPD) technique in nine Turkish commercial rapeseed varieties. The RAPD primers (10-mer oligonucleotides) produced 51 scorable loci, 31 loci of which were polymorphic (60.78%) and 20 loci (39.22%) were monomorphic The RAPD bands were scored as binary matrix data and were analysed using POPGENE version 1.32. At locus level, the values of genetic diversity within population (Hs) and total (HT) were 0.15 and 0.19 respectively. The genetic differentiation (GST) and the gene flow (Nm) values between the populations were 0.20 and 2.05 respectively. The mean number of alleles (na), the mean number of effective alleles (nae), and the mean value of genetic diversity (He) were 2.00, 1.26, and 0.19 respectively. According to Pearson’s correlation, multiple regression and principal component analyses, eco-geographical conditions in combination had significant effect on genetic indices of commercial B. napus L. varieties were discussed.

Comparing the nitrogen and phosphorus requirements of canola and wheat for grain yield and quality

2009 ◽  
Vol 60 (6) ◽  
pp. 566 ◽  
Author(s):  
R. F. Brennan ◽  
M. D. A. Bolland
Keyword(s):  
Grain Yield ◽  
Protein Concentration ◽  
Grain Production ◽  
Wheat Grain ◽  
Nitrogen And Phosphorus ◽  
Brassica Napus L ◽  
Crop Species ◽  
Oil Concentration ◽  
Four Levels ◽  
Yield Responses

Canola (oilseed rape, Brassica napus L.) is now grown in rotation with spring wheat (Triticum aestivum L.) on the predominantly sandy soils of south-western Australia. For both crop species, fertiliser nitrogen (N) and phosphorus (P) need to be applied for profitable grain production. The fertiliser N requirements have been determined separately for canola or wheat when adequate P was applied. By contrast, the fertiliser P requirements of the 2 species have been compared in the same experiment when adequate N was applied and showed that canola consistently required ~25–60% less P than wheat to produce 90% of the maximum grain yield. We report results of a field experiment conducted at 7 sites from 2000 to 2003 in the region to compare grain yield responses of canola and wheat to application of N and P in the same experiment. Four levels of N (0–138 kg N/ha as urea [46% N]) and 6 levels of P (0–40 kg P/ha as superphosphate [9.1%P]) were applied. Significant grain yield responses to applied N and P occurred for both crop species at all sites of the experiment, and the N × P interaction for grain production was always significant. To produce 90% of the maximum grain yield, canola required ~40% more N (range 16–75%) than wheat, and ~25% less P (range 12–43%) than wheat. For both crop species at 7 sites, applying increasing levels of N had no significant effect on the level of P required for 90% of maximum grain yield, although at 1 site the level of P required to achieve the target yield for both crop species when no N was applied (nil-N treatment) was significantly lower than for the other 3 treatments treated with N. For both crop species at all 7 sites, applying increasing levels of P increased the level of N required for 90% of the maximum grain yield. Fertiliser P had no significant effect on protein concentration in canola and wheat grain, and oil concentration in canola grain. As found in previous studies, application of increasing levels of N decreased oil concentration while increasing protein concentration in canola grain, and increased protein concentration in wheat grain. The N × P interaction was not significant for protein or oil concentration in grain. Protein concentrations in canola grain were about double those found in wheat grain.

scholarly journals Comparing the phosphorus requirements of wheat, lupin, and canola

2008 ◽  
Vol 59 (11) ◽  
pp. 983 ◽  
Author(s):  
M. D. A. Bolland ◽  
R. F. Brennan
Keyword(s):  
Growth Rates ◽  
Triticum Aestivum L ◽  
Yield Response ◽  
Brassica Napus L ◽  
Crop Species ◽  
P Concentration ◽  
Large Yield ◽  
P Content ◽  
Yield Responses ◽  
Phosphorus Requirements

Spring wheat (Triticum aestivum L.), lupin (Lupinus angustifolius L.), and canola (Brassica napus L.) are the major crop species grown in rotation on the predominantly sandy soils of south-western Australia. Comparisons among the species for yield responses to applied phosphorus (P), effects of applied P on growth rates of shoots, P response efficiency for shoot and grain production, and the pattern for accumulation of P into shoots during growth and into grain at maturity are rare, or are not known, and were quantified in the glasshouse study reported here. Size and P content (P concentration multiplied by yield) of sown seed were in the order canola < wheat < lupin. Therefore, yield responses to applied P were first observed at ~10 days after sowing (DAS) for canola, ~17 DAS for wheat, and ~60 DAS for lupin. Lupin shoots showed no yield response to applied P at the first harvest at 51 DAS. Otherwise all species showed large yield, P concentration, and P content responses to applied P for all harvests at 51, 78, 87, 101, 121, and 172 DAS. To produce 90% of the maximum grain yield, the relevant data for cropping, lupin required ~67% less P than wheat, canola required ~40% less P than wheat, and canola required ~75% more P than lupin. Growth rates, and P response efficiency, were generally largest for canola, followed by wheat, then lupin. For shoots, P accumulation was in the order lupin > wheat > canola at 51 DAS, canola > wheat > lupin at 78 and 87 DAS, canola > wheat = lupin at 101 DAS, and all 3 species were about similar at 121 DAS. For accumulation of P into shoots plus grain at maturity (172 DAS) the order was canola > lupin > wheat, and for grain only was canola > wheat = lupin.

scholarly journals Research on the Allelophatic Effect Between the Invasive Species Ambrosia Artemisiifolia L. (“Floarea Pustei”) and Some Agricultural Crops

1970 ◽  
Vol 66 (1) ◽  
Author(s):  
Nicolae HODIŞAN ◽  
Gavrilă MORAR ◽  
Cristina-Maria NEAG
Keyword(s):  
Invasive Species ◽  
Triticum Aestivum L ◽  
Ambrosia Artemisiifolia ◽  
Allelopathic Effect ◽  
Hordeum Vulgare L ◽  
Brassica Napus L ◽  
Crop Species ◽  
Inhibiting Effect ◽  
Secale Cereale L ◽  
Germination And Growth

The paper presents the results of the allelopathic effect on the germination and growth of plants, immediately after springing, in the interaction between the invasive species Ambrosia artemisiifolia L. (common ragweed) and five crop species: wheat (Triticum aestivum L.), rye (Secale cereale L.), barley (Hordeum vulgare L.), rape (Brassica napus L.) and lucerne (Medicago sativa). The tests consisted in applying treatments with aqueous extracts obtained from young Ambrosia artemisiifolia L. plants, as well as from different vegetative organs harvested from mature plants (roots, leaves and seeds). The results show a highly significant inhibiting effect on the germination of wheat, rye, barley and rape seeds and an insignificant one in lucerne seeds. A strong inhibiting effect upon the growth of plants in early stages of vegetation was established in wheat and rape and a stimulation of growth in the same stage of vegetation in barley and lucerne.

scholarly journals Evolution and development of the paired spikelet trait in maize and other grasses (Poaceae)

2017 ◽  
Author(s):  
◽  
Eden A. Johnson
Keyword(s):  
Central Zone ◽  
Grass Species ◽  
Plant Structure ◽  
Crop Species ◽  
Meristem Size ◽  
Evolution And Development ◽  
Complex Organization ◽  
The Family ◽  
Meristem Maintenance

Meristems are composed of groups of tightly-regulated cells that coordinate the number of stem cells in the central zone of the meristem with the number of cells migrating to organ primordia in the peripheral zones of the meristem. Mutations disturbing this balance can cause significant changes to the resulting plant structure. In order to buffer the meristem against drastic changes, it is necessary to have overlapping pathways regulating meristem maintenance and meristem determinacy. Furthermore, components and functions of these pathways are often conserved between plant lineages. While flowering in Arabidopsis occurs from the direct transition of the shoot apical meristem to a floral meristem, the grasses develop numerous axillary meristems that specify several different structures (i.e. inflorescences, branches, and grass spikelets), making the maintenance and identity of these meristems interesting areas of research. Thus, my first chapter reviews the mechanisms by which meristems are initiated and maintained through the complex organization of meristem size and determinacy pathways in plants. Members of the grass family develop inflorescences composed of spikelets, which house the inconspicuous grass flowers. One compelling difference between grass species is that, while the majority of the approximately 12,000 taxa develop single spikelets, there are also a significant number of species that produce paired spikelets. It is widely acknowledged that the subfamily Panicoideae contains many paired spikelet species, namely within three tribes that include significant crop species (i.e., maize, sorghum, setaria, and switchgrass). However, there are many cereal crops (i.e., wheat and rice) which develop single spikelets, indicating that the paired spikelet trait may be important to study as a way to increase yield in single spikelet species. My research into the semi-dominant maize mutant Suppressor of sessile spikelet1 (Sos1) investigated the developmental basis behind the paired spikelet trait. While maize plants normally bear paired spikelets, Sos1 mutants develop single spikelets in the tassel inflorescence and alternating rows of single kernels in the ear. A previous study examined this mutant early in development and revealed that the mutant inflorescence meristems were smaller than normal, which suggested a potential role for the sos1 gene in meristem maintenance. The objective of my second chapter was to summarize what is known about the Sos1 mutant, and uncover the role of the sos1 gene in meristem maintenance. Double mutants were generated between Sos1 and members of the CLAVATA signaling pathway, which is a well-known pathway shown to be critical for maintaining meristem size in maize, Arabidopsis, and other plant systems. Analysis of these double mutants indicated that sos1 functions in this important meristem maintenance pathway. Research into the Sos1 maize mutant also prompted a more in-depth investigation into the evolution of the paired spikelet trait in the grasses, which was previously only assumed to occur in three tribes of the Panicoideae subfamily. The objective of my third chapter was to determine if the paired spikelet trait evolved multiple times in the grasses. My analysis highlighted the fact that the paired spikelet trait originated before the Panicoideae subfamily. This study uncovered that species developing paired spikelets are prevalent in more grass lineages than previously shown, and highlights a pattern of repeated losses and origins of the paired spikelet trait throughout the family.

scholarly journals Evaluation of Organic Spring Cover Crop Termination Practices to Enhance Rolling/Crimping

Agronomy ◽  
2019 ◽  
Vol 9 (9) ◽  
pp. 519
Author(s):  
Price ◽  
Duzy ◽  
McElroy ◽  
Li
Keyword(s):  
Cover Crop ◽  
Conservation Tillage ◽  
The United States ◽  
Clove Oil ◽  
Hairy Vetch ◽  
Brassica Napus L ◽  
Crop Species ◽  
Cereal Rye ◽  
Crimson Clover ◽  
Clear Plastic

With organic farming hectarage and cover crop interest increasing throughout the United States, effectively timed cover crop termination practices are needed that can be utilized in organic conservation tillage production systems. Four commercially available termination treatments approved by Organic Materials Review Institute (OMRI) were evaluated, immediately following mechanical termination with a cover crop roller/crimper and compared to a synthetic herbicide termination to access termination rates. Treatments included rolling/crimping followed by (1) 20% vinegar solution (28 L a.i. ha−1 acetic acid), (2) 2.5 L a.i. ha−1 45% cinnamon (Cinnamomum verum L.) oil (cinnamaldehyde, eugenol, eugenol acetate)/45% clove oil (eugenol, acetyl eugenol, caryophyllene) mixture, (3) 0.15 mm clear polyethylene sheeting applied with edges manually tucked into the soil for 28 days over the entire plot area (clear plastic), (4) broadcast flame emitting 1100 °C applied at 1.2 k/h (flame), (5) glyphosate applied at 1.12 kg a.i. ha−1 (this non-OMRI-approved, non-organic conservation tillage cover crop termination standard practice was included to help ascertain desiccation, regrowth, and economics), and (6) a non-treated control. Five cover crop species were evaluated: (1) hairy vetch (Vicia villosa Roth), (2) crimson clover (Trifolium incarnatum L.), (3) cereal rye (Secale cereale L.), (4) Austrian winter pea (Pisum sativum L.), and (5) rape (Brassica napus L.). Three termination timings occurred at four-week intervals beginning mid-March each year. In April or May, organic producers are most likely to be successful using a roller crimper as either a broadcast flamer for terminating all winter covers evaluated, or utilizing clear plastic for hairy vetch, winter peas, and cereal rye. Ineffectiveness and regrowth concerns following cover crop termination in March are substantial. Commercially available vinegar and cinnamon/clove oil solutions provided little predictable termination, and producers attempting to use these OMRI-approved products will likely resort to cover crop incorporation, or mowing, to terminate covers if no other practice is readily available.

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