scholarly journals The Antimicrobial Resistance Pattern of Klebsiella pneumonia Isolated from the Clinical Specimens in Duhok City in Kurdistan Region of Iraq

2020 ◽  
Vol 24 (2) ◽  
Author(s):  
Ibrahim Abdulqader Naqid ◽  
Nawfal Rasheed Hussein ◽  
Amer A Balatay ◽  
Kurdistan Abdullah Saeed ◽  
Hiba A Ahmed
Keyword(s):  
Antimicrobial Resistance ◽  
High Resistance ◽  
Clinical Sample ◽  
Resistance Rate ◽  
Clinical Samples ◽  
Resistance Pattern ◽  
Klebsiella Pneumonia ◽  
Global Public Health ◽  
Clinical Specimens ◽  
Sensitivity Pattern

Background: Klebsiella pneumoniae is an important opportunistic enteric bacterial strain, which is a major cause of pneumonia and urinary tract infection. Antimicrobial resistance is an increasingly severe threat to the Global Public Health, requiring immediate action across governmental sectors and communities. Objectives: The present study aimed to determine the sensitivity pattern of K. pneumonia isolated from various clinical specimens for common antibiotics in Duhok City, Iraq. Methods: This study was conducted in Duhok City during January 2017-February 2019 on 130 clinical samples of urine, blood, sputum, wound swabs, central venous lines, and oral swabs. K. pneumoniae strains were confirmed and tested in terms of susceptibility to various antimicrobial drugs using the VITEK-2 compact system. Results: In total, 130 positive K. pneumoniae cultures from various clinical samples were examined. The isolates were more predominant in the females (n = 99; 76.2%) compared to males (n = 31; 23.8%). The antibiotic resistance rate of K. pneumoniae varied among different isolate clinical sample sources. Overall, high resistance rates were recorded for ampicillin (96.9%), ceftriaxone (65.8%), and cefepime (60.8%). However, ertapenem (93.8%) and imipenem (82.3%) showed the highest susceptibility rate against the isolates. Conclusions: According to the results, K. pneumoniae isolated from various clinical specimens varied in terms of the antibiotic susceptibility pattern with high resistance to common antibiotics, particularly ampicillin. Ertapenem and imipenem were the most effective antibiotics against the isolates. Our findings could help physicians and clinicians to select appropriate antimicrobial therapies in the region.

scholarly journals Antimicrobial Resistance Pattern of Enterobacter Species Isolated from Different Clinical Specimens in a Tertiary Care Hospital of Bangladesh

2019 ◽  
Vol 13 (2) ◽  
pp. 3-6
Author(s):  
Mashrura Quraishi ◽  
Ahmed Abu Saleh ◽  
Chandan Kumar Roy ◽  
Fatima Afroz ◽  
GM Mohiuddin
Keyword(s):  
Antimicrobial Resistance ◽  
High Resistance ◽  
Antimicrobial Susceptibility ◽  
Susceptibility Testing ◽  
Antimicrobial Susceptibility Testing ◽  
Diffusion Method ◽  
Resistance Pattern ◽  
Clinical Specimens ◽  
Enterobacter Species ◽  
Resistance Rates

The present study was undertaken to determine the antimicrobial resistance pattern of Enterobacter species to guide the clinician in selecting the best antimicrobial agent for an individual patient. A total of 50 clinical isolates of Enterobacter species were collected from different clinical specimens at the microbiology laboratory of BSMMU between August, 2018 and September, 2019. The two main species of Enterobacter, E.cloacae and E.aerogenes were identified by biochemical tests. Antimicrobial susceptibility testing was performed by Kirby Bauer disc diffusion method and reported according to CLSI guidelines. Majority (56%) of the isolated Enterobacter were E.cloacae, 40% were E.aerogenes and 4% were other species. The Enterobacter isolates showed relatively high resistance rates to the cephalosporins including cefoxitin (82%), cefixime (62%), ceftazidime (46%) and ceftriaxone (46%). Resistance to the carbapenems and aminoglycosides was relatively low. The high resistance rates of Enterobacter species to multiple antibiotics makes it necessary for antimicrobial susceptibility testing to be conducted prior to antibiotic prescription. Bangladesh J Med Microbiol 2019; 13 (2): 3-6

scholarly journals Antimicrobial Resistance Pattern In Escherichia Coli Isolated From Children Admitted At Selected Health Care Facilities In Moshi Municipality, Tanzania

2019 ◽  
Author(s):  
Mzelifa Daud ◽  
Balthazar Nyombi
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Resistance ◽  
High Resistance ◽  
Health Centre ◽  
Resistance Rate ◽  
Health Care Facilities ◽  
Diffusion Method ◽  
Resistance Pattern ◽  
Rectal Swabs ◽  
Agar Media

Abstract Background The emergence of antimicrobial resistance is a great challenge in preventing, controlling and treatment Gram-negative bacteria and has become a significant public health threat worldwide. The study aimed at determination of the prevalence and pattern of antimicrobial resistance in Escherichia coli isolated in children admitted at health facilities in Moshi municipality, Tanzania. Methodology A descriptive cross sectional study was carried out on archived rectal swabs collected from 282 children aged 3 to 168 months admitted at Kilimanjaro Christian Medical Centre (KCMC), Mawenzi Regional Referral Hospital and Pasua health centre in Moshi municipality. Rectal swabs were cultured on MacConkey agar media. The isolated Escherichia coli was identified and antimicrobial susceptibility test was done by using Kirby–Bauer disc diffusion method on Muller Hinton agar media. Results Escherichia coli were isolated in all 282 cultured archived rectal swabs and all isolates were susceptible to amikacin and meropenem. ESBL producers were 13.1% and 86.9% were non-ESBLs. Conclusion High resistance rate were observed on ampicillin, ceftriaxone, ceftazidime, cefuroxime, trimethoprim and ciprofloxacin among ESBL producing Escherichia coli. Among non ESBL producing Escherichia coli high resistance rate were observed on ampicillin and trimethoprim; however all isolates were sensitive to amikacin and meropenem.

scholarly journals Evaluating the antimicrobial resistance patterns and MDR frequency in Escherichia coli strains isolated from clinical specimens taken from patients in Iran: 2017–2019

2020 ◽  
Author(s):  
Zahra Norouzi Bazgir ◽  
Mehrdad Gholami ◽  
Hamid Reza Goli
Keyword(s):  
Antimicrobial Resistance ◽  
High Resistance ◽  
Control Measures ◽  
Teaching Hospitals ◽  
Diffusion Method ◽  
Clinical Samples ◽  
Clinical Specimens ◽  
E Coli ◽  
Infection Control Measures ◽  
Resistance Patterns

Abstract Objectives The purpose of this study was to evaluate the antimicrobial resistance and frequency of MDR strains in E. coli isolated from patients in two hospitals of Iran. In this descriptive-analytical study, 13322 clinical specimens were collected from two teaching hospitals. The E. coli isolates were identified using standard bacteriological methods. Then, the resistance patterns of the isolates were analyzed by disk agar diffusion method according to the CLSI.Results Out of 13322 clinical samples, 964 (7.23%) isolates of E. coli were identified. In both hospitals, high resistance to ampicillin and cefalexin was presented in 621 (64.4%) and 402 (41.7%) isolates, respectively. The highest antibiotic resistance was observed in burn unit, burn ICU and burn restoration section, while all 8 strains which were isolated from the neonatal-ICU, were sensitive to all tested antibiotics except cefalexin, nitrofurantoin, nalidixic acid, and ampicillin. Also, the bacteria isolated from urine, wound, stool, and blood samples were resistant to all tested antibiotics. Increased resistance to different antibiotics in burn hospital has created increasing concern. Very high resistance to some antibiotics indicates that these drugs are misused in therapeutic centers and highlights that infection control measures should be arranged in the ICUs of our hospitals.

scholarly journals ANTIMICROBIAL RESISTANCE PATTERN IN ESCHERICHIA COLI ISOLATED FROM CHILDREN ADMITTED AT SELECTED HEALTH CARE FACILITIES IN MOSHI MUNICIPALITY, TANZANIA

2019 ◽  
Author(s):  
Mzelifa Daud ◽  
Balthazar Nyombi
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Resistance ◽  
High Resistance ◽  
Health Centre ◽  
Resistance Rate ◽  
Health Care Facilities ◽  
Diffusion Method ◽  
Resistance Pattern ◽  
Rectal Swabs ◽  
Agar Media

Abstract Background The emergence of antimicrobial resistance is a great challenge in preventing, controlling and treatment Gram-negative bacteria and has become a significant public health threat worldwide. The study aimed at determination of the prevalence and pattern of antimicrobial resistance in Escherichia coli isolated in children admitted at health facilities in Moshi municipality, Tanzania. Methodology A descriptive cross sectional study was carried out on archived rectal swabs collected from 282 children aged 3 to 168 months admitted at Kilimanjaro Christian Medical Centre (KCMC), Mawenzi Regional Referral Hospital and Pasua health centre in Moshi municipality. Rectal swabs were cultured on MacConkey agar media. The isolated Escherichia coli was identified and antimicrobial susceptibility test was done by using Kirby–Bauer disc diffusion method on Muller Hinton agar media. Results Escherichia coli were isolated in all 282 cultured archived rectal swabs and all isolates were susceptible to amikacin and meropenem. ESBL producers were 13.1% and 86.9% were non-ESBLs. Conclusion High resistance rate were observed on ampicillin, ceftriaxone, ceftazidime, cefuroxime, trimethoprim and ciprofloxacin among ESBL producing Escherichia coli. Among non ESBL producing Escherichia coli high resistance rate were observed on ampicillin and trimethoprim; however all isolates were sensitive to amikacin and meropenem.

scholarly journals ANTIBACTERIAL RESISTANCE PATTERN OF PSEUDOMONAS AERUGINOSA ISOLATED FROM CLINICAL SAMPLES AT A GENERAL HOSPITAL IN PADANG, WEST SUMATRA, INDONESIA

2017 ◽  
Vol 10 (8) ◽  
pp. 158
Author(s):  
Rustini Rustini ◽  
Jamsari Jamsari ◽  
Marlina Marlina ◽  
Nasrul Zubir ◽  
Yori Yuliandra
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Bacterial Resistance ◽  
Clinical Sample ◽  
Opportunistic Pathogen ◽  
Positive Control ◽  
Diffusion Method ◽  
Clinical Samples ◽  
Resistance Pattern ◽  
Tract Infections ◽  
Almost All

Objectives: Pseudomonas aeruginosa is an opportunistic pathogen that has an innate resistance to some antibiotics. This bacterium is one of the mostcommon causes of nosocomial infections that include surgical wound infections, burns, and urinary tract infections. The bacteria have been reportedlyresistant to many antibiotics and have developed multidrug resistance (MDR). The objective of the study was to determine the resistance pattern ofP. aeruginosa isolated from clinical samples of patients against some major antibiotics.Methods: Isolates of P. aeruginosa were obtained from clinical sample of urine, sputum, swabs, pus, feces, and blood and cultured in cetrimide agar. P.aeruginosa ATCC 27853 was used as a positive control. The antibacterial susceptibility testing was conducted against 13 antibiotics: Ceftazidime, cefotaxime,ceftriaxone, cefoperazone, ciprofloxacin, levofloxacin, ofloxacin, gentamicin, amikacin, piperacillin, ticarcillin, meropenem, and imipenem. The examinationwas carried out using agar diffusion method of Kirby-Bauer and following the standards from Clinical and Laboratory Standards Institute (CLSI).Results: The results showed that bacterial resistance was established against all tested antibiotics. The highest number of resistance was shownagainst ceftriaxone (44.21%), whereas the most susceptibility was exhibited against amikacin (only 9.47% of resistance). MDR P. aeruginosa (MDRPA)was detected on almost all clinical samples tested, except the feces. The sample with the highest percentage of MDRPA was the pus.Conclusion: The study concludes that the most effective antibiotic against P. aeruginosa is amikacin (91.51%), whereas the most resistance is exhibited to ceftriaxone (43.16%).

scholarly journals Is Antimicrobial Resistance Pattern of Enteric Fever Changing in Kathmandu Valley?

2013 ◽  
Vol 32 (3) ◽  
pp. 221-228
Author(s):  
Ganesh Kumar Rai ◽  
Subhana Karki ◽  
Bina Prajapati
Keyword(s):  
Antimicrobial Resistance ◽  
Nalidixic Acid ◽  
Enteric Fever ◽  
Public Health Problem ◽  
Salmonella Typhi ◽  
Resistance Pattern ◽  
Kathmandu Valley ◽  
Sensitivity Testing ◽  
Antimicrobial Sensitivity ◽  
Sensitivity Pattern

Introduction: Enteric fever is a public health problem in developing countries including Nepal. Antimicrobial sensitivity pattern of Salmonella sp causing enteric fever is changing over time and also differs according to geographical location. Periodic surveillance of antimicrobial resistance of Salmonella sp is mandatory for management of enteric fever rationally. The objective of this study was to analyze the resistance pattern of Salmonella sp to commonly used antimicrobials. Materials and Methods: This was a retrospective study undertaken reviewing the records of blood isolates of Salmonella sp over one year period at Kanti Children’s Hospital, Nepal. Antimicrobial sensitivity testing was done following Kirby Bauer’s disk diffusion technique using Muller Hinton agar. Results: Salmonella typhi was found to be 100% resistance to ampicillin and resistance to nalidixic acid and ciprofloxacin was also high. The resistance to chloramphenicol and ofloxacin was low. No resistance was found to amoxicillin, ceftriaxone, cefotaxime and amikacin. However, among the sensitive isolates of Salmonella typhi intermediate sensitivity to ceftriaxone and ceftazidime was reported to be high. Among the isolates of S paratyphi A, the resistance to nalidixic acid was 100% followed by 75% to ciprofloxacin. No resistance was found to chloramphenicol, ceftriaxone, cefixime, amikacin and low resistance to amoxicillin and ofloxacin. Among the sensitive isolates of S paratyphi A, majority of them were only moderately sensitive to cefotaxime and ceftazidime and about 1/3 of the organisms had only intermediate sensitivity to ceftriaxone. Conclusiion: Commonly used parenteral third generation cephalosporins, first line drugs like chloramphenicol and amoxicillin and ofloxacin among fluoroquinolones were found to be effective in vitro in treating enteric fever. However, Salmonella typhi was found to be highly resistant to the most frequently used drugs like ciprofloxacin and cefixime and Salmonella paratyphi A to ciprofloxacin. A comparative chart of antimicrobial sensitivity of enteric fever in children over 10 year period from different hospitals of Kathmandu valley is also presented in the study. DOI: http://dx.doi.org/10.3126/jnps.v32i3.6625 J. Nepal Paediatr. SocVol.32(3) 2012 221-228

scholarly journals The face of hypervirulent Klebsiella pneumoniae isolated from clinical samples of two Iranian teaching hospitals

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Rahimeh Sanikhani ◽  
Mohammad Moeinirad ◽  
Hamid Solgi ◽  
Azar Hadadi ◽  
Fereshteh Shahcheraghi ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Antimicrobial Resistance ◽  
Klebsiella Pneumoniae ◽  
High Resistance ◽  
Multidrug Resistant ◽  
Teaching Hospitals ◽  
Clinical Samples ◽  
Clonal Relatedness ◽  
The Face ◽  
Global Concern

AbstractHypervirulent Klebsiella pneumoniae (hvKp) has emerged as a pathogen of global concern. In this study, both phenotypic and genotypic tests were used to detect hvKp. Antimicrobial resistance profiles and clonal relatedness of clinical isolates were also determined. We found that 34.2% (163/477) of the isolates were tellurite resistant, and among them 102 hvKp isolates detected with iucA or iutA or peg-344 as molecular markers. The blaSHV (80.4%), followed by blaCTX-M-15 (76.5%) and blaTEM (67.6%), blaOXA-48 (53.9%), and blaNDM-1 (32.3%) were detected, while blaKPC-1 was not present in any hvKp isolates. It was found that the majority of hvKp isolates belonged to capsular serotype K20 and ompK36 group C, which is related to clonal group (CG) 23 (e.g. ST23). A high percentage of multidrug-resistant hvKp (76.6%) and high resistance to imipenem (67%) indicated a serious problem that should be addressed in the clinical setting.

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