scholarly journals Determination of aflatoxin B1 in food products in Thailand

2014 ◽  
Vol 13 (53) ◽  
pp. 4761-4765 ◽  
Author(s):  
Charoenpornsook K. ◽  
Kavisarasai P.
Keyword(s):  
Aflatoxin B1 ◽  
Food Products

scholarly journals Determination of aflatoxins in food products by the ELISA method

2013 ◽  
Vol 19 (No. 1) ◽  
pp. 8-12 ◽  
Author(s):  
J. Leszczyńska ◽  
J. MasŁowska ◽  
A. Owczarek ◽  
U. Kucharska
Keyword(s):  
Aflatoxin B1 ◽  
Dairy Products ◽  
Total Content ◽  
Food Products ◽  
Aflatoxin M1 ◽  
Elisa Method ◽  
Cereal Samples

To determine the total content of aflatoxins, aflatoxin B1 and aflatoxin M1 in food the ELISA method was used. Milk, dairy products and cereal samples were mainly investigated. A few samples were found to be contaminated with aflatoxins. A great usability of the ELISA method for aflatoxin determination in food was established. Selectivity and sensitivity of the method is reported.

scholarly journals Comparison of the efficiency of photoionization at atmospheric pressure and electrospray ionization on the example of some aflatoxins and trichothecenes

2020 ◽  
Vol 64 (12) ◽  
pp. 113-119
Author(s):  
Aisylu Z. Mukharlyamova ◽  
◽  
Aleksandr M. Saifutdinov ◽  
Elvira R. Rakhmetova ◽  
Aygul G. Mukhammetshina ◽  
...  
Keyword(s):  
Electrospray Ionization ◽  
Aflatoxin B1 ◽  
Atmospheric Pressure ◽  
Raw Materials ◽  
Food Products ◽  
Mass Spectrometric ◽  
Spectrometric Analysis ◽  
Ionization Source ◽  
Mass Spectrometric Detector

The article presents the results of comparing the effectiveness of modern ionization methods used in chromatomass spectrometric analysis: photoionization at atmospheric pressure (APPI) and electrospray ionization (ESI) for the determination of aflatoxin B1 and T-2 toxins related to the main natural pollutants of agricultural feed, food raw materials and food products. Aflatoxin B1 is a member of the aflatoxin family, which in their chemical structure are derivatives of substituted coumarins or furocoumarins. The peculiarity of aflatoxins is that in the process of technological or culinary processing, they are practically not destroyed, while being the strongest hepatocancerogens that cause irreversible liver damage. T-2 toxin refers to trichothecene mycotoxins, in the structure of which there is a trichothecene ring containing a multiple bond and an epoxy group. Most trichothecene mycotoxins are slightly toxic compounds, but T-2 toxin is deadly, having a negative effect on immunocompetent organs, hematopoietic organs, and the gastrointestinal tract, increasing the risk of internal hemorrhage. Due to the impossibility of preventing contamination of agricultural feed, food raw materials and food products with mycotoxins, their strict control is necessary. Chromatomass spectrometry is one of the methods that provide high sensitivity and reliability for the determination of mycotoxins Methods of ionization sources, such as electrospray and photoionization at atmospheric pressure during mass spectrometric detection of mycotoxins by high-performance liquid chromatography (HPLC-MS/MS), are evaluated. In order to increase the sensitivity of the determinations, the optimal conditions of the quadrupole-time-of-flight mass spectrometric detector were selected and the maximum intensity responses of the analytes were obtained. During the experiment, it was found that the intensity of the signal with a photoionization source obtained by analyzing the T-2 toxin is higher than the signal obtained by using an electrospray ionization source.

Development of ultrasensitive direct chemiluminescent enzyme immunoassay for determination of aflatoxin B1 in food products

Talanta ◽  
2013 ◽  
Vol 107 ◽  
pp. 25-29 ◽  
Author(s):  
Feng-Yih Yu ◽  
Anastasia V. Gribas ◽  
Marina M. Vdovenko ◽  
Ivan Yu. Sakharov
Keyword(s):  
Enzyme Immunoassay ◽  
Aflatoxin B1 ◽  
Food Products ◽  
Chemiluminescent Enzyme Immunoassay

Determination of quaternary ammonium compounds in food products by the method of ultra-performance liquid chromatography/high resolution mass-spectrometry

2020 ◽  
Vol 86 (8) ◽  
pp. 23-31
Author(s):  
V. G. Amelin ◽  
D. S. Bolshakov
Keyword(s):  
Mass Spectrometry ◽  
High Resolution ◽  
Quaternary Ammonium ◽  
High Resolution Mass Spectrometry ◽  
Food Products ◽  
Quaternary Ammonium Compounds ◽  
High Resolution Mass ◽  
Ammonium Compounds ◽  
Resolution Mass

The goal of the study is developing a methodology for determination of the residual amounts of quaternary ammonium compounds (QAC) in food products by UHPLC/high-resolution mass spectrometry after water-acetonitrile extraction of the determined components from the analyzed samples. The identification and determination of QAC was carried out on an «UltiMate 3000» ultra-high-performance liquid chromatograph (Thermo Scientific, USA) equipped with a «maXis 4G» high-resolution quadrupole-time-of-flight mass spectrometric detector and an ion spray «ionBooster» source (Bruker Daltonics, Germany). Samples of milk, cheese (upper cortical layer), dumplings, pork, chicken skin and ground beef were used as working samples. Optimal conditions are specified for chromatographic separation of the mixture of five QAC, two of them being a mixture of homologues with a linear structure (including isomeric forms). The identification of QAC is carried out by the retention time, exact mass of the ions, and coincidence of the mSigma isotopic distribution. The limits for QAC detection are 0.1 – 0.5 ng/ml, the determination limits are 1 ng/ml for aqueous standard solutions. The determinable content of QAC in food products ranges within 1 – 100 ng/g. The results of analysis revealed the residual amount of QAC present in all samples, which confirms data of numerous sources of information about active use of QAC-based disinfectants in the meat and dairy industry. The correctness of the obtained results is verified by introduction of the additives in food products at a level of 10 ng/g for each QAC. The relative standard deviation of the analysis results does not exceed 0.18. The duration of the analysis is 30 – 40 min.

scholarly journals Current Strategies for Studying the Natural and Synthetic Bioactive Compounds in Food by Chromatographic Separation Techniques

Processes ◽  
2021 ◽  
Vol 9 (7) ◽  
pp. 1100
Author(s):  
Wioletta Parys ◽  
Małgorzata Dołowy ◽  
Alina Pyka-Pająk
Keyword(s):  
Gas Chromatography ◽  
Bioactive Compounds ◽  
Residue Analysis ◽  
Food Products ◽  
Vital Role ◽  
Bioactive Molecules ◽  
Validation Parameters ◽  
Layer Chromatography ◽  
Natural And Synthetic

The present study summarizes the new strategies including advanced equipment and validation parameters of liquid and gas chromatography methods i.e., thin-layer chromatography (TLC), column liquid chromatography (CLC), and gas chromatography (GC) suitable for the identification and quantitative determination of different natural and synthetic bioactive compounds present in food and food products, which play an important role in human health, within the period of 2019–2021 (January). Full characteristic of some of these procedures with their validation parameters is discussed in this work. The present review confirms the vital role of HPLC methodology in combination with different detection modes i.e., HPLC-UV, HPLC-DAD, HPLC-MS, and HPLC-MS/MS for the determination of natural and synthetic bioactive molecules for different purposes i.e., to characterize the chemical composition of food as well as in the multi-residue analysis of pesticides, NSAIDs, antibiotics, steroids, and others in food and food products.

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