In vitro assessments of white-spot lesions treated with NaF plus tricalcium phosphate (TCP) toothpastes using synchrotron radiation micro computed tomography (SR micro-CT)

Objective: The aim of this study was to use Micro computed tomography (micro-CT) to evaluate the influence of the type of vehicle associated with calcium hydroxide on its ability to penetrate simulated lateral canals. Materials and methods: 30 acrylic blocks with simulated lateral canals comprising apical, middle and cervical thirds were used in the in vitro study. The blocks were divided into 3 groups (n = 10) according to the type of vehicle used (chlorhexidine, distilled water and propylene glycol) in the calcium hydroxide slurry, which was inserted in the respective group of simulated canals with a K# 30 file and then agitated with an ultrasonic tip. The blocks were scanned by micro-computed tomography (micro-CT) before and after insertion of the medication. The images obtained were reconstructed and analyzed to obtain the initial volume of lateral canals and the volume of medication that penetrated into them. Results: In the intragroup analysis, both distilled water and chlorhexidine 2% were observed to present statistical difference in all thirds of the canal. Propylene glycol showed no intragroup difference. In the inter-group analysis, the propylene glycol paste presented higher values of penetration into the simulated lateral canals than the other groups (p <0.05). Conclusion: Propylene glycol used as vehicle of the calcium hydroxide paste provided better penetration results in simulated lateral canals.

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Three-Dimensional Quantification of Calcium Salt-Composite Resorption (CSC) In Vitro by Micro-computed Tomography (Micro-CT)

JOM ◽

10.1007/s11837-014-0884-4 ◽

2014 ◽

Vol 66 (4) ◽

pp. 559-565 ◽

Cited By ~ 1

Author(s):

T. Winkler ◽

X. Y. Dai ◽

G. Mielke ◽

S. Vogt ◽

H. Buechner ◽

...

Keyword(s):

Computed Tomography ◽

Calcium Salt ◽

Three Dimensional ◽

Micro Ct ◽

Micro Computed Tomography

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Detection of Marginal Leakage of Class V Restorations In Vitro by Micro–Computed Tomography

Operative Dentistry ◽

10.2341/12-182-l ◽

2014 ◽

Vol 39 (2) ◽

pp. 174-180 ◽

Cited By ~ 13

Author(s):

XY Zhao ◽

SB Li ◽

LJ Gu ◽

Y Li

Keyword(s):

Computed Tomography ◽

Resin Composite ◽

Nitrate Solution ◽

Three Dimensional ◽

Micro Ct ◽

Micro Computed Tomography ◽

Class V ◽

Enamel Structure ◽

Significant Difference

SUMMARY This in vitro study evaluated the efficacy of micro–computed tomography (CT) in marginal leakage detection of Class V restorations. Standardized Class V preparations with cervical margins in dentin and occlusal margins in enamel were made in 20 extracted human molars and restored with dental bonding agents and resin composite. All teeth were then immersed in 50% ammoniacal silver nitrate solution for 12 hours, followed by a developing solution for eight hours. Each restoration was scanned by micro-CT, the depth of marginal silver leakage in the central scanning section was measured, and the three-dimensional images of the silver leakage around each restoration were reconstructed. Afterward, all restorations were cut through the center and examined for leakage depth using a microscope. The silver leakage depth of each restoration obtained by the micro-CT and the microscope were compared for equivalency. The silver leakage depth in cervical walls observed by micro-CT and microscope showed no significant difference; however, in certain cases the judgment of leakage depth in the occlusal wall in micro-CT image was affected by adjacent enamel structure, providing less leakage depth than was observed with the microscope (p<0.01). Micro-CT displayed the three-dimensional image of the leakage around the Class V restorations with clear borders only in the dentin region. It can be concluded that micro-CT can detect nondestructively the leakage around a resin composite restoration in two and three dimensions, with accuracy comparable to that of the conventional microscope method in the dentin region but with inferior accuracy in the enamel region.

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New Technique for Detecting Cracked Teeth and Evaluating the Crack Depth by Contrast-enhanced Cone Beam Computed Tomography: an in Vitro Study

10.21203/rs.3.rs-1110699/v1 ◽

2021 ◽

Author(s):

Jie Zhou ◽

Jieni Fu ◽

Mo Xiao ◽

Yangyang Lv ◽

Fei Wu ◽

...

Keyword(s):

Computed Tomography ◽

Cone Beam Computed Tomography ◽

Crack Depth ◽

In Vitro Study ◽

New Technique ◽

Cone Beam ◽

Micro Ct ◽

Micro Computed Tomography ◽

Contrast Enhanced

Abstract Background: Develop a new technique based on contrast-enhanced cone beam computed tomography (CBCT) to improve the detection of cracked teeth and the accuracy of crack depth evaluation in vitro. Methods: We developed an in vitro artificial simulation model of cracked teeth. Pre-experimental CBCT (pre-CBCT), and micro-computed tomography (micro-CT) were first performed for all cracked teeth (n = 31). Contrast-enhanced CBCT was then performed by infiltrating the crack with ioversol under vacuum conditions. The results of pre-CBCT, micro-CT, and contrast-enhanced CBCT were recorded. SPSS v.26.0 software (IBM Corp, Somers, NY) and R software , version 3.6.0 (R Foundation for Statistical Computing; http://www.r-project.org/) and RStudio 1.1.463 (RStudio, PBC, Boston, MA, US) were used to perform the statistical analysis for the study.Results: The sensitivities of pre-CBCT and contrast-enhanced CBCT were 48.4%, and 67.7%, respectively. ICC value of crack depth as measured by pre-CBCT and contrast-enhanced CBCT was 0.847 (95% confidence interval:0.380-0.960; P < 0.001). The areas under ROC curves (AUC) of pre-CBCT and contrast-enhanced CBCT were different, the AUC of pre-CBCT was 0.958 (P = 0.000, 95% CI :0.843-1.074), and the AUC of enhanced CBCT was 0.979 (P = 0.000, 95% CI :0.921-1.037), and the difference was not statistically significant (Z=-0.707, P = 0.480). The ICC value of crack depth as measured by contrast-enhanced CBCT and micro-CT was 0.753 (95% CI: 0.248-0.911; P < 0.001). Conclusion: Contrast-enhanced CBCT under vacuum conditions with a contrast medium can only significantly improve the cracks detection rate of cracked teeth, but not measure the crack depths accurately.

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Cortical and trabecular bone parameters from HR-Pqct images at the Tibia: a local comparison with synchrotron radiation micro-computed tomography

Bone Abstracts ◽

10.1530/boneabs.1.pp316 ◽

2013 ◽

Author(s):

Agnes Ostertag ◽

Francoise Peyrin ◽

Sylvie Fernandez ◽

Jean-Denis Laredo ◽

Vernejoul Marie-Christine De ◽

...

Keyword(s):

Computed Tomography ◽

Synchrotron Radiation ◽

Trabecular Bone ◽

Micro Computed Tomography ◽

Bone Parameters ◽

Local Comparison

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Micro-Computed Tomography Analysis of Subchondral Bone Regeneration Using Osteochondral Scaffolds in an Ovine Condyle Model

Applied Sciences ◽

10.3390/app11030891 ◽

2021 ◽

Vol 11 (3) ◽

pp. 891

Author(s):

Taylor Flaherty ◽

Maryam Tamaddon ◽

Chaozong Liu

Keyword(s):

Computed Tomography ◽

Bone Regeneration ◽

Subchondral Bone ◽

Volume Ratio ◽

Bone Volume ◽

Osteochondral Defects ◽

Micro Ct ◽

Micro Computed Tomography ◽

Trabecular Thickness ◽

Osteochondral Scaffold

Osteochondral scaffold technology has emerged as a promising therapy for repairing osteochondral defects. Recent research suggests that seeding osteochondral scaffolds with bone marrow concentrate (BMC) may enhance tissue regeneration. To examine this hypothesis, this study examined subchondral bone regeneration in scaffolds with and without BMC. Ovine stifle condyle models were used for the in vivo study. Two scaffold systems (8 mm diameter and 10 mm thick) with and without BMC were implanted into the femoral condyle, and the tissues were retrieved after six months. The retrieved femoral condyles (with scaffold in) were examined using micro-computed tomography scans (micro-CT), and the micro-CT data were further analysed by ImageJ with respect to trabecular thickness, bone volume to total volume ratio (BV/TV) ratio, and degree of anisotropy of bone. Statistical analysis compared bone regeneration between scaffold groups and sub-set regions. These results were mostly insignificant (p < 0.05), with the exception of bone volume to total volume ratio when comparing scaffold composition and sub-set region. Additional trends in the data were observed. These results suggest that the scaffold composition and addition of BMC did not significantly affect bone regeneration in osteochondral defects after six months. However, this research provides data which may guide the development of future treatments.

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Using micro-computed tomography to reveal the anatomy of adult Diaphorina citri Kuwayama (Insecta: Hemiptera, Liviidae) and how it pierces and feeds within a citrus leaf

Scientific Reports ◽

10.1038/s41598-020-80404-z ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Javier Alba-Tercedor ◽

Wayne B. Hunter ◽

Ignacio Alba-Alejandre

Keyword(s):

Computed Tomography ◽

Significant Advance ◽

Micro Ct ◽

Diaphorina Citri ◽

Ct Reconstruction ◽

Internal Anatomy ◽

Micro Computed Tomography ◽

Citrus Leaf ◽

Teaching Aids ◽

Adult Feeding

AbstractThe Asian citrus psyllid (ACP), Diaphorina citri, is a harmful pest of citrus trees that transmits Candidatus Liberibacter spp. which causes Huanglongbing (HLB) (citrus greening disease); this is considered to be the most serious bacterial disease of citrus plants. Here we detail an anatomical study of the external and internal anatomy (excluding the reproductive system) using micro-computed tomography (micro-CT). This is the first complete 3D micro-CT reconstruction of the anatomy of a psylloid insect and includes a 3D reconstruction of an adult feeding on a citrus leaf that can be used on mobile devices. Detailed rendered images and videos support first descriptions of coxal and scapus antennal glands and sexual differences in the internal anatomy (hindgut rectum, mesothoracic ganglion and brain). This represents a significant advance in our knowledge of ACP anatomy, and of psyllids in general. Together the images, videos and 3D model constitute a unique anatomical atlas and are useful tools for future research and as teaching aids.

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Poly(δ-valerolactone)/Poly(ethylene-co-vinylalcohol)/β-Tricalcium Phosphate Composite as Scaffolds: Preparation, Properties, and In Vitro Amoxicillin Release

Polymers ◽

10.3390/polym13010046 ◽

2020 ◽

Vol 13 (1) ◽

pp. 46

Author(s):

Mohammed Badwelan ◽

Mohammed Alkindi ◽

Osama Alghamdi ◽

Waseem Sharaf Saeed ◽

Abdel-Basit Al-Odayni ◽

...

Keyword(s):

Hybrid Materials ◽

Tricalcium Phosphate ◽

Drug Carrier ◽

Human Mesenchymal Stem Cells ◽

In Vitro Study ◽

Micro Computed Tomography ◽

Scanning Calorimetry ◽

Sem Images ◽

Poly Ethylene

Two poly(δ-valerolactone)/poly(ethylene-co-vinylalcohol)/β-tricalcium phosphate (PEVAL/PDVAL/β-TCP) composites containing an equal ratio of polymer and filled with 50 and 70 wt% of β-TCP microparticles were prepared by the solvent casting method. Interconnected pores were realized using the salt leached technique, and the porosity of the resulted composites was evaluated by the scanning electron microscopy (SEM) method. The homogeneity of the hybrid materials was investigated by differential scanning calorimetry (DSC) and X-ray diffraction (XRD) analysis. The prepared materials’ SEM images showed interconnected micropores that respond to the conditions required to allow their uses as scaffolds. The porosity of each scaffold was determined from micro computed tomography (micro-CT) data, and the analysis of the mechanical properties of the prepared materials was studied through the stress-strain compressive test. The proliferation test results used human mesenchymal stem cells (MSCs) to grow and proliferate on the different types of prepared materials, reflecting that the hybrid materials were non-toxic and could be biologically acceptable scaffolds. The antibacterial activity test revealed that incorporation of amoxicillin in the specimens could inhibit the bacterial growth of S. aureus. The in vitro study of the release of amoxicillin from the PEVAL/PDVAL/amoxicillin and PEVAL/PDVAL/β-TCP/amoxicillin drug carrier systems in pH media 7.4, during eight days, gave promising results, and the antibiotic diffusion in these scaffolds obeys the Fickian model.

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MicroCT protocols for scanning of embryos and juvenile Hexaplex trunculus v1

10.17504/protocols.io.bxw4ppgw ◽

2021 ◽

Author(s):

Eva Chatzinikolaou ◽

Kleoniki Keklikoglou

Keyword(s):

Computed Tomography ◽

High Resolution ◽

3D Imaging ◽

Imaging Technique ◽

Ct Scanning ◽

Micro Ct ◽

Micro Computed Tomography ◽

Gastropod Species ◽

Biological Studies ◽

Hexaplex Trunculus

Micro-computed tomography (micro-CT) is a high-resolution 3D-imaging technique which is now increasingly applied in biological studies focusing on taxonomy and functional morphology. The creation of virtual representations of specimens can increase availability of otherwise underexploited and inaccessible samples. This protocol aims to standardise micro-CT scanning procedures for embryos and juveniles of the marine gastropod species Hexaplex trunculus.

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