Influence of the calcium hydroxide paste vehicle on penetration into lateral canals

Objective: The aim of this study was to use Micro computed tomography (micro-CT) to evaluate the influence of the type of vehicle associated with calcium hydroxide on its ability to penetrate simulated lateral canals. Materials and methods: 30 acrylic blocks with simulated lateral canals comprising apical, middle and cervical thirds were used in the in vitro study. The blocks were divided into 3 groups (n = 10) according to the type of vehicle used (chlorhexidine, distilled water and propylene glycol) in the calcium hydroxide slurry, which was inserted in the respective group of simulated canals with a K# 30 file and then agitated with an ultrasonic tip. The blocks were scanned by micro-computed tomography (micro-CT) before and after insertion of the medication. The images obtained were reconstructed and analyzed to obtain the initial volume of lateral canals and the volume of medication that penetrated into them. Results: In the intragroup analysis, both distilled water and chlorhexidine 2% were observed to present statistical difference in all thirds of the canal. Propylene glycol showed no intragroup difference. In the inter-group analysis, the propylene glycol paste presented higher values of penetration into the simulated lateral canals than the other groups (p <0.05). Conclusion: Propylene glycol used as vehicle of the calcium hydroxide paste provided better penetration results in simulated lateral canals.

Download Full-text

Micro-Computed Tomography as a Tool to Investigate the Deformation Behavior of Particulate-Filled Composite Materials

Journal of Engineering Materials and Technology ◽

10.1115/1.4037658 ◽

2017 ◽

Vol 140 (2) ◽

Cited By ~ 3

Author(s):

Kenan Cinar ◽

Ibrahim Guven

Keyword(s):

Computed Tomography ◽

Composite Materials ◽

Displacement Field ◽

Deformation Behavior ◽

Mass Fraction ◽

Indentation Test ◽

Micro Ct ◽

Micro Computed Tomography ◽

Before And After ◽

Filled Composite

Micro-computed tomography (CT) was used as a tool to investigate the deformation behavior of particulate-filled composite materials. Three different shapes of glass fillers (spherical, flake, and fiber) and filler mass fractions (5%, 10%, and 15%) were introduced to the epoxy resin. Rockwell hardness H scale indentation test was used to deform the composite material. The composite materials were scanned before and after the indentation test by using micro-CT. Displacement field for each filler type and mass fraction were measured through correlation of before and after scan data. The effects of filler type and mass fraction on the internal displacement field were investigated. It was also demonstrated that micro-CT can be used as a tool to create realistic representative volume elements (RVEs) for particulate-filled composite materials instead of randomly distributed particles within the matrix material.

Download Full-text

Three-Dimensional Quantification of Calcium Salt-Composite Resorption (CSC) In Vitro by Micro-computed Tomography (Micro-CT)

JOM ◽

10.1007/s11837-014-0884-4 ◽

2014 ◽

Vol 66 (4) ◽

pp. 559-565 ◽

Cited By ~ 1

Author(s):

T. Winkler ◽

X. Y. Dai ◽

G. Mielke ◽

S. Vogt ◽

H. Buechner ◽

...

Keyword(s):

Computed Tomography ◽

Calcium Salt ◽

Three Dimensional ◽

Micro Ct ◽

Micro Computed Tomography

Download Full-text

In vitro assessments of white-spot lesions treated with NaF plus tricalcium phosphate (TCP) toothpastes using synchrotron radiation micro computed tomography (SR micro-CT)

Journal of Dentistry and Oral Hygiene ◽

10.5897/jdoh2013.0105 ◽

2014 ◽

Vol 6 (1) ◽

pp. 10-21 ◽

Cited By ~ 1

Author(s):

Asaizumi Makoto ◽

Uesugi Kentaro ◽

Hoshino Masato ◽

Kato Tomoaki ◽

C. Mackey Allen ◽

...

Keyword(s):

Computed Tomography ◽

Synchrotron Radiation ◽

Tricalcium Phosphate ◽

White Spot ◽

White Spot Lesions ◽

Micro Ct ◽

Micro Computed Tomography

Download Full-text

Detection of Marginal Leakage of Class V Restorations In Vitro by Micro–Computed Tomography

Operative Dentistry ◽

10.2341/12-182-l ◽

2014 ◽

Vol 39 (2) ◽

pp. 174-180 ◽

Cited By ~ 13

Author(s):

XY Zhao ◽

SB Li ◽

LJ Gu ◽

Y Li

Keyword(s):

Computed Tomography ◽

Resin Composite ◽

Nitrate Solution ◽

Three Dimensional ◽

Micro Ct ◽

Micro Computed Tomography ◽

Class V ◽

Enamel Structure ◽

Significant Difference

SUMMARY This in vitro study evaluated the efficacy of micro–computed tomography (CT) in marginal leakage detection of Class V restorations. Standardized Class V preparations with cervical margins in dentin and occlusal margins in enamel were made in 20 extracted human molars and restored with dental bonding agents and resin composite. All teeth were then immersed in 50% ammoniacal silver nitrate solution for 12 hours, followed by a developing solution for eight hours. Each restoration was scanned by micro-CT, the depth of marginal silver leakage in the central scanning section was measured, and the three-dimensional images of the silver leakage around each restoration were reconstructed. Afterward, all restorations were cut through the center and examined for leakage depth using a microscope. The silver leakage depth of each restoration obtained by the micro-CT and the microscope were compared for equivalency. The silver leakage depth in cervical walls observed by micro-CT and microscope showed no significant difference; however, in certain cases the judgment of leakage depth in the occlusal wall in micro-CT image was affected by adjacent enamel structure, providing less leakage depth than was observed with the microscope (p<0.01). Micro-CT displayed the three-dimensional image of the leakage around the Class V restorations with clear borders only in the dentin region. It can be concluded that micro-CT can detect nondestructively the leakage around a resin composite restoration in two and three dimensions, with accuracy comparable to that of the conventional microscope method in the dentin region but with inferior accuracy in the enamel region.

Download Full-text

New Technique for Detecting Cracked Teeth and Evaluating the Crack Depth by Contrast-enhanced Cone Beam Computed Tomography: an in Vitro Study

10.21203/rs.3.rs-1110699/v1 ◽

2021 ◽

Author(s):

Jie Zhou ◽

Jieni Fu ◽

Mo Xiao ◽

Yangyang Lv ◽

Fei Wu ◽

...

Keyword(s):

Computed Tomography ◽

Cone Beam Computed Tomography ◽

Crack Depth ◽

In Vitro Study ◽

New Technique ◽

Cone Beam ◽

Micro Ct ◽

Micro Computed Tomography ◽

Contrast Enhanced

Abstract Background: Develop a new technique based on contrast-enhanced cone beam computed tomography (CBCT) to improve the detection of cracked teeth and the accuracy of crack depth evaluation in vitro. Methods: We developed an in vitro artificial simulation model of cracked teeth. Pre-experimental CBCT (pre-CBCT), and micro-computed tomography (micro-CT) were first performed for all cracked teeth (n = 31). Contrast-enhanced CBCT was then performed by infiltrating the crack with ioversol under vacuum conditions. The results of pre-CBCT, micro-CT, and contrast-enhanced CBCT were recorded. SPSS v.26.0 software (IBM Corp, Somers, NY) and R software , version 3.6.0 (R Foundation for Statistical Computing; http://www.r-project.org/) and RStudio 1.1.463 (RStudio, PBC, Boston, MA, US) were used to perform the statistical analysis for the study.Results: The sensitivities of pre-CBCT and contrast-enhanced CBCT were 48.4%, and 67.7%, respectively. ICC value of crack depth as measured by pre-CBCT and contrast-enhanced CBCT was 0.847 (95% confidence interval:0.380-0.960; P < 0.001). The areas under ROC curves (AUC) of pre-CBCT and contrast-enhanced CBCT were different, the AUC of pre-CBCT was 0.958 (P = 0.000, 95% CI :0.843-1.074), and the AUC of enhanced CBCT was 0.979 (P = 0.000, 95% CI :0.921-1.037), and the difference was not statistically significant (Z=-0.707, P = 0.480). The ICC value of crack depth as measured by contrast-enhanced CBCT and micro-CT was 0.753 (95% CI: 0.248-0.911; P < 0.001). Conclusion: Contrast-enhanced CBCT under vacuum conditions with a contrast medium can only significantly improve the cracks detection rate of cracked teeth, but not measure the crack depths accurately.

Download Full-text

Micro-Computed Tomography Analysis of Subchondral Bone Regeneration Using Osteochondral Scaffolds in an Ovine Condyle Model

Applied Sciences ◽

10.3390/app11030891 ◽

2021 ◽

Vol 11 (3) ◽

pp. 891

Author(s):

Taylor Flaherty ◽

Maryam Tamaddon ◽

Chaozong Liu

Keyword(s):

Computed Tomography ◽

Bone Regeneration ◽

Subchondral Bone ◽

Volume Ratio ◽

Bone Volume ◽

Osteochondral Defects ◽

Micro Ct ◽

Micro Computed Tomography ◽

Trabecular Thickness ◽

Osteochondral Scaffold

Osteochondral scaffold technology has emerged as a promising therapy for repairing osteochondral defects. Recent research suggests that seeding osteochondral scaffolds with bone marrow concentrate (BMC) may enhance tissue regeneration. To examine this hypothesis, this study examined subchondral bone regeneration in scaffolds with and without BMC. Ovine stifle condyle models were used for the in vivo study. Two scaffold systems (8 mm diameter and 10 mm thick) with and without BMC were implanted into the femoral condyle, and the tissues were retrieved after six months. The retrieved femoral condyles (with scaffold in) were examined using micro-computed tomography scans (micro-CT), and the micro-CT data were further analysed by ImageJ with respect to trabecular thickness, bone volume to total volume ratio (BV/TV) ratio, and degree of anisotropy of bone. Statistical analysis compared bone regeneration between scaffold groups and sub-set regions. These results were mostly insignificant (p < 0.05), with the exception of bone volume to total volume ratio when comparing scaffold composition and sub-set region. Additional trends in the data were observed. These results suggest that the scaffold composition and addition of BMC did not significantly affect bone regeneration in osteochondral defects after six months. However, this research provides data which may guide the development of future treatments.

Download Full-text

Using micro-computed tomography to reveal the anatomy of adult Diaphorina citri Kuwayama (Insecta: Hemiptera, Liviidae) and how it pierces and feeds within a citrus leaf

Scientific Reports ◽

10.1038/s41598-020-80404-z ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Javier Alba-Tercedor ◽

Wayne B. Hunter ◽

Ignacio Alba-Alejandre

Keyword(s):

Computed Tomography ◽

Significant Advance ◽

Micro Ct ◽

Diaphorina Citri ◽

Ct Reconstruction ◽

Internal Anatomy ◽

Micro Computed Tomography ◽

Citrus Leaf ◽

Teaching Aids ◽

Adult Feeding

AbstractThe Asian citrus psyllid (ACP), Diaphorina citri, is a harmful pest of citrus trees that transmits Candidatus Liberibacter spp. which causes Huanglongbing (HLB) (citrus greening disease); this is considered to be the most serious bacterial disease of citrus plants. Here we detail an anatomical study of the external and internal anatomy (excluding the reproductive system) using micro-computed tomography (micro-CT). This is the first complete 3D micro-CT reconstruction of the anatomy of a psylloid insect and includes a 3D reconstruction of an adult feeding on a citrus leaf that can be used on mobile devices. Detailed rendered images and videos support first descriptions of coxal and scapus antennal glands and sexual differences in the internal anatomy (hindgut rectum, mesothoracic ganglion and brain). This represents a significant advance in our knowledge of ACP anatomy, and of psyllids in general. Together the images, videos and 3D model constitute a unique anatomical atlas and are useful tools for future research and as teaching aids.

Download Full-text

Effects of pre-extraction intermittent PTH administration on extraction socket healing in bisphosphonate administered ovariectomized rats

Scientific Reports ◽

10.1038/s41598-020-79787-w ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Jae-Young Kim ◽

Hyo-Won Jang ◽

Jung-In Kim ◽

In-Ho Cha

Keyword(s):

Tooth Extraction ◽

Bisphosphonate Therapy ◽

Ovariectomized Rats ◽

Control Group ◽

Micro Ct ◽

Micro Computed Tomography ◽

Extraction Socket ◽

Before And After ◽

Socket Healing ◽

Positive Effect

AbstractThe purpose of this study was to investigate the effect of administering intermittent parathyroid hormone (iPTH) before tooth extraction versus after tooth extraction on the risk of developing MRONJ in experimental animal model. Twenty-five ovariectomized rats received 6 weeks of bisphosphonate therapy. They were classified into 3 groups, based on the timing of the medication, as Control, Pre-PTH and Post-PTH groups. For Control group, normal saline was administered before and after tooth extraction. iPTH was administered during 4 weeks before tooth extraction for Pre-PTH group and after tooth extraction for Post-PTH group. The animals were euthanized 8 weeks after tooth extraction. Macroscopic, histological, micro-computed tomography (micro-CT), and histomorphometric examinations were conducted. The incidences of impaired healing were 11.11% both in Pre-PTH and Post-PTH groups, which was lower than the Control group (42.86%). Bone healing in the extraction socket, based on micro-CT and histomorphometry evaluations, was best in Post-PTH and worst in Control group. The Pre-PTH group showed moderate healing pattern. Despite of limitations in this study, the authors identified Pre-PTH group seems to have positive effect on extraction socket healing. With regard to timing, administering iPTH after tooth extraction was superior to applying it before tooth extraction.

Download Full-text

MicroCT protocols for scanning of embryos and juvenile Hexaplex trunculus v1

10.17504/protocols.io.bxw4ppgw ◽

2021 ◽

Author(s):

Eva Chatzinikolaou ◽

Kleoniki Keklikoglou

Keyword(s):

Computed Tomography ◽

High Resolution ◽

3D Imaging ◽

Imaging Technique ◽

Ct Scanning ◽

Micro Ct ◽

Micro Computed Tomography ◽

Gastropod Species ◽

Biological Studies ◽

Hexaplex Trunculus

Micro-computed tomography (micro-CT) is a high-resolution 3D-imaging technique which is now increasingly applied in biological studies focusing on taxonomy and functional morphology. The creation of virtual representations of specimens can increase availability of otherwise underexploited and inaccessible samples. This protocol aims to standardise micro-CT scanning procedures for embryos and juveniles of the marine gastropod species Hexaplex trunculus.

Download Full-text