scholarly journals Optimization of conteining dermal coverages production for burn wound treatment at in vivo model

2018 ◽  
Vol 22 ◽  
pp. 287-292
Author(s):  
L. L. Matsevych ◽  
A. Ye. Papuga ◽  
T. P. Ruban ◽  
T. V. Beregova ◽  
L. L. Lukash
Keyword(s):  
Cell Suspension ◽  
Positive Control ◽  
Negative Control ◽  
Skin Substitute ◽  
In Vivo Model ◽  
Control Group ◽  
Burn Wound ◽  
Properties Of Coatings ◽  
Significant Difference

Aim. The aim was to estimate the influence of cell suspension quality on the therapeutic efficiency of cell-containing dermal coverages in animal model in vivo. Methods. We carried out the application of gel wound coverages with different quality of cellular compound on the third degree burns of ICR line mice. In the negative control group animals were treated with fresh medium-containing gel, and in positive control – by gel containing high quality cell suspension. Photo fixation of burn wound status was carried out once a day. The results were estimated by ANOVA approach. Results. There was a statistically significant difference of burn wounds development and healing between positive control and both experimental groups. The decreasing of alive cell fraction in prepared gel within 5 hours has been shown as well. Conclusions. It has been shown the dependence of wound healing properties of coatings containing cells on the cell compound quality, in particular, from the viability and integrity of cells. These results are important for developing of clinical protocols using such cell‑containing dermal equivalents. Keywords: burn wound, dermal equivalent, stem cells, skin substitute, tissue engineering.

scholarly journals Investigation of cell-derived preparations efficacy for the treatment of severe burn wounds

1970 ◽  
pp. 232-236
Author(s):  
L. L. Matsevych ◽  
O. Ye. Papuga ◽  
T. P. Ruban ◽  
L. L. Lukash
Keyword(s):  
Biologically Active ◽  
Skin Substitute ◽  
Control Group ◽  
Burn Wound ◽  
Properties Of Coatings ◽  
Burn Wounds ◽  
Cultural Medium ◽  
Significant Difference ◽  
Active Substances

Aim. The aim was to estimate the efficiency of cell-containing and non-cellular dermal coverages in animal model in vivo. Methods. We carried out the application of gel wound coverages with three different cell lines and cultural medium conditioned by these cells on the third degree burns of ICR line mice. In the control group animals were treated with fresh medium-containing gel. Photo fixation of burn wound status was carried out once a day. The results were estimated by ANOVA approach. Results. There was a statistically significant difference of burn wounds development and healing within three experimental groups treated with cell suspensions of different lines. Cell-free gels with media conditioned by these cells were shown to be effective as well. Conclusions. It has been shown the dependence of wound healing properties of coatings containing cells from the origin of these cells. The effectiveness of these coverages is supposed to be intermediated by biologically active substances secreted by the cells.Keywords: burn wound, dermal equivalent, stem cells, skin equivalent, skin substitute, tissue engineering.

Potential Antiseptic of Rhodomyrtus tomentosa leaves extract on Healing wound in Male Wistar rats

2021 ◽  
pp. 3143-3149
Author(s):  
Endang Sri Purwanti Ningsih ◽  
Noorlaila Noorlaila ◽  
Ikhwan Rizki Muhammad ◽  
Windy Yuliana Budianto
Keyword(s):  
Wound Healing ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Wound Care ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Rhodomyrtus Tomentosa

Background: The process of wound healing is influenced by various factors such as age, hormones, and wound care. Wound care is done to accelerate wound healing which can be done by various methods, one of them is traditional care. Traditional wound care can use medicinal plants. Rhodomyrtus tomentosa is a medicinal plant that has an antioxidant, anti-inflammatory, antitumor and antibacterial content. Thus this study aims to evaluate the effectiveness of the antiseptic solution of the Rodhomyrtus tomentosa leaf extract on wound healing in male Wistar rats. Method: this research is pure experimental research with post test only control group design. Thirty male white rats were divided into five groups, namely negative control, positive control, Rhodomyrtus tomentosa leaf extract 15%, 30%, and 60%. Rhodomyrtus tomentosa leaf extraction was carried out by maceration method with 70% ethano solvent. The extraction results are divided into 3 concentrations (15%, 30% and 60%). The wound healing process was evaluated by measuring the length of the wound manually from 0 to 10 days in each group. Meanwhile, the number of fibroblast cells was calculated through hematoxylin eosin (HE) staining and observed using an Olympus CX41 microscope with a 10x magnification and objective lens magnification in 3 fields. Result: There was a significant difference in the reduction in wound length (p =< 0,000) between the five experimental groups (Rhodomyrtus tomentosa leaf extract solution 15%, 30% and 60%, negative control and positive control. Solution of rhodomyrtus tomentosa leaf extract accelerated the increase in the number of fibroblasts compared to the negative control group (p = 0.003), but did not make a difference (p = 0.403) with the positive control group. Rhodomyrtus tomentosa leaf extraction solution had the same microscopic effect on the number of fibroblasts with a positive control group given 0.9% NaCl solution. Conclusion: There was a significant difference in the number of fibroblasts between all groups, but no difference in wound healing length.

scholarly journals Evaluation of the Sealing Ability of Three Obturation Techniques Using a Glucose Leakage Test

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
Katarzyna Olczak ◽  
Halina Pawlicka
Keyword(s):  
Continuous Wave ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Thermal Methods ◽  
Root Canals ◽  
Anterior Teeth ◽  
Sealing Ability ◽  
Significant Difference

The aim of this study was to evaluate the sealing ability of three different canal filling techniques. Sixty-four roots of extracted human maxillary anterior teeth were prepared using ProTaper® rotary instruments. The specimens were then randomly divided into 3 experimental groups (n=16) and 2 control groups (n=8). The root canals were filled using cold lateral compaction (CLC group), continuous wave condensation technique using the Elements Obturation Unit® (EOU group), and ProTaper obturators (PT group). For the negative control group, 8 roots were filled using lateral compaction as in the CLC group, and the teeth were covered twice with a layer of nail varnish (NCG group). Another 8 roots were filled using lateral compaction, but without sealer, and these were used as the positive control (PCG group). A glucose leakage model was used for quantitative evaluation of microleakage for 24 hours and 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, and 12 weeks. No significant difference in the cumulative amount of leakage was found between the three experimental groups at all observation times. The lateral condensation of cold gutta-percha can guarantee a similar seal of canal fillings as can be achieved by using thermal methods, in the round canals.

scholarly journals A Digital Microscopic Inspection of Dentinal Defects after Using Endodontic Retreatment Files

2021 ◽  
Vol 2021 ◽  
pp. 1-6
Author(s):  
Ammar AbuMostafa ◽  
Hala Almoqayyad ◽  
Al-Omari Mohammad
Keyword(s):  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Filling Material ◽  
Root Canals ◽  
Root Canal Retreatment ◽  
Significant Difference ◽  
Digital Microscope ◽  
Microscopic Inspection

Aim. The study aimed at evaluating the incidence of dentinal defects after root canal retreatment with ProTaper Universal retreatment (PTUR) and XP-endo Shaper and Finisher R (XP). Materials and Methods. Sixty extracted single-rooted human premolars were selected and divided into 4 groups of 15 teeth each. In the negative control group, the teeth were left unprepared. In the positive control group, the teeth were prepared with ProTaper Next and obturated with no further retreatment. In the PTUR and XP groups, the teeth were prepared and obturated followed by removal of the filling material at body temperature using PTUR and XP instruments, respectively. The roots were then sectioned at 3, 6, and 9 mm from the apex and observed under a digital microscope to detect defects. Results. PTUR group showed significantly higher ( p value <0.05) incidence of defects than the other groups. Comparison of no defects versus defects between groups in different areas of root canals demonstrated significant difference among the groups in the apical and cervical regions. Conclusion. Within the limitations of the present study, PTUR files created significantly more dentinal defects than XP files, with most of those defects at the cervical and apical areas of the root canals.

scholarly journals In-vivo Antiplasmodial Effect of Dihydroartemisinin-Piperaquine-Nitrofurantoin on Plasmodium berghei- Infected Mice

2021 ◽  
pp. 12-20
Author(s):  
Udeme O. Georgewill ◽  
Festus Azibanigha Joseph ◽  
Elias Adikwu
Keyword(s):  
Plasmodium Berghei ◽  
Blood Cells ◽  
Hematological Parameters ◽  
White Blood Cells ◽  
Positive Control ◽  
Negative Control ◽  
Antiplasmodial Activity ◽  
Significant Difference ◽  
Tract Infections

Nitrofurantoin (NT) used for the treatment of urinary tract infections may have antiplasmodial activity. Dihydroartemisinin-piperaquine (DP) is an artemisinin based combination therapy used for the treatment of malaria. This study evaluated the antiplasmodial effect of dihydroartemisinin-piperaquine-nitrofurantoin (DP-NT) on mice infected with Plasmodium berghei. Adult Swiss albino mice (30-35 g) of both sexes were used. The mice were randomly grouped, inoculated with Plasmodium berghei, and treated orally with DP (1.7/13.7 mg/kg), NT (57.1 mg/kg) and DP-NT (1.71/13.7/ 57.1 mg/kg), respectively using curative, prophylactic and suppressive tests. The negative control was orally treated with normal saline (0.3 mL), while the positive control was orally treated with chloroquine CQ (10mg/kg). After treatment, blood samples were collected and evaluated for percentage parasitemia, inhibitions and hematological parameters. Liver samples were evaluated for histological changes. The mice were observed for mean survival time (MST). Treatment with DP-NT decreased parasitemia levels when compared to individual doses of DP and NT with significant difference observed at p<0.05. DP-NT prolonged MST when compared to individual doses of DP and NT with significant difference observed at p<0.05. The decrease in packed cell volume, red blood cells, hemoglobin and increase in white blood cells in parasitized mice were significantly restored by DP-NT  when compared to individual doses of DP and NT with difference observed at p<0.05. DP-NT eradicated liver Plasmodium parasite.  NT remarkably increased the antiplasmodial activity of DP. DP-NT may be used for the treatment of malaria.

scholarly journals Study the effect of the mixture alcoholic extract of Peganum harmala seeds and cones of Cupressus sempervirens and their effect on viability of protoscolices of Echinococcus granulosus in vivo

2011 ◽  
Vol 5 (2) ◽  
pp. 44-52
Author(s):  
Noor Nihad Baker ◽  
Fawzia Ahmed AL-Shanawi
Keyword(s):  
Hydatid Cyst ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Alcoholic Extract ◽  
Peganum Harmala ◽  
Cupressus Sempervirens ◽  
Positive Group ◽  
Plant Mixture

this study included the using of the mixture of alcoholic extract Peganum harmala seeds and cones of Cupressus sempervirens at concentrations (1+50) mgml. And then experimentation on the mice injected with protoscolices and its comparison with the mice injected with only protoscolices (as positive control group), and the mice injected with normal saline (as negative control) to investigate the effect of plant mixture in vivo, it appeared of getting the reduction of hydatid cyst with percentage 100% in processed group with the mixture compared with positive group as its absence of the hydatid cyst in processed group. The lowering significantly occurred in the averages of the weights of the liver and spleen and the averages of its distension in processed groups and about of the positive group and which was approach to the negative group. Also study the tissular changes occurred in the liver and spleen, in the liver it occurred of changes in the liver cell and increase in the number of the kupffer cell as a defensive in the processed group were less than what it appeared in the positive control, but the spleen, it appeared the dilation of the whit pulp and the appearance of the cell composing of the hemic platelets (megakaryocyte cells) in the mice processed in comparison with negative control. These changes were of less acuity in the group processed. Thus from the results of this study at appeared the possibility of using the mixture in vivo in successful and safe way by it a capability of initiating the immunity system to the inhibition of the protoscolices and prevent the development of the secondary hydatid cyst in vivo without causing the negative side effect.

Comparison of Biocompatibility of Experimental Tricalcium Phosphate Cement versus Biodentin and Mineral Trioxide Aggregate used for Furcation Perforation Repair (in vivo study)

2021 ◽  
Vol 15 (1) ◽  
pp. 532-538
Author(s):  
Mostafa M. El-Bialy ◽  
Magdy M. Ali ◽  
Engy M. Kataia ◽  
Reham S. El Nemr ◽  
Reham Hassan
Keyword(s):  
Tricalcium Phosphate ◽  
Mineral Trioxide Aggregate ◽  
Positive Control ◽  
Control Group ◽  
Tooth Survival ◽  
Significant Difference ◽  
Operative Evaluation ◽  
Root Canal System ◽  
Bone Deposition

Background: The interaction between the root canal system and the oral cavity caused by iatrogenic perforations significantly affects the treatment outcome and tooth survival. Objectives: This study was directed to compare the biocompatibility of an experimental tricalcium phosphate cement versus biodentine and mineral Trioxide aggregate used for furcation perforation repair in dogs. Methods: Perforations were done in 60 teeth (premolars and molars) of six adult dogs. Animals were divided randomly into 3 equal groups of 2 animals each according to the post-operative evaluation period of 1 week, 1 month, and 3-months. Each group was further subdivided into 4 subgroups according to either being repaired with the experimental tricalcium phosphate cement (n=6), or Biodentine (n=6), Mineral Trioxide Aggregate (MTA) (n=6), and positive control(n=2). After evaluation periods, tissue blocks were harvested and histologically examined. Results: No statistically significant difference was found regarding bone deposition scores and inflammatory reaction in the three groups after 1 week, 1 month, or 3 months. All three groups showed a statistically significant difference between all three time periods. Regardless of the repair material used, inflammation scores at 1-week showed the highest scores, decreasing over time except for the control group. Conclusion: The experimental material could be considered as a successful treatment option for repairing furcation perforation.

scholarly journals Cleaved CD31 as a target for in vivo molecular imaging of inflammation

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Jonathan Vigne ◽  
Sylvie Bay ◽  
Rachida Aid-Launais ◽  
Guillaume Pariscoat ◽  
Guillaume Rucher ◽  
...  
Keyword(s):  
Molecular Imaging ◽  
Molecular Form ◽  
Positive Control ◽  
Negative Control ◽  
Stability Study ◽  
Biodistribution Studies ◽  
Wide Range ◽  
Significant Difference

AbstractThere is a need for new targets to specifically localize inflammatory foci, usable in a wide range of organs. Here, we hypothesized that the cleaved molecular form of CD31 is a suitable target for molecular imaging of inflammation. We evaluated a bioconjugate of D-P8RI, a synthetic peptide that binds all cells with cleaved CD31, in an experimental rat model of sterile acute inflammation. Male Wistar rats were injected with turpentine oil into the gastrocnemius muscle two days before 99mTc-HYNIC-D-P8RI (or its analogue with L-Proline) SPECT/CT or [18F]FDG PET/MRI. Biodistribution, stability study, histology, imaging and autoradiography of 99mTc-HYNIC-D-P8RI were further performed. Biodistribution studies revealed rapid elimination of 99mTc-HYNIC-D-P8RI through renal excretion with almost no uptake from most organs and excellent in vitro and in vivo stability were observed. SPECT/CT imaging showed a significant higher 99mTc-HYNIC-D-P8RI uptake compared with its analogue with L-Proline (negative control) and no significant difference compared with [18F]FDG (positive control). Moreover, autoradiography and histology revealed a co-localization between 99mTc-HYNIC-D-P8RI uptake and inflammatory cell infiltration. 99mTc-HYNIC-D-P8RI constitutes a new tool for the detection and localization of inflammatory sites. Our work suggests that targeting cleaved CD31 is an attractive strategy for the specific in vivo imaging of inflammatory processes.

scholarly journals HISTOPATHOLOGICAL STRUCTURE OF WHITE RATS LIVER AFTER GIVING ANT NEST EXTRACT DUE TO INDUCED PARACETAMOL TOXIC DOSE

2019 ◽  
pp. 14
Author(s):  
I Made Merdana ◽  
I Made Kardena ◽  
Ketut Budiasa ◽  
I Made Dodi Gunawan
Keyword(s):  
Rat Liver ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Toxic Dose ◽  
White Rats ◽  
Significant Difference ◽  
Ant Nest ◽  
Histopathologic Changes

This study aim was to determine the influence ant nest plant extract (Myrmecodia pendans) on histopathological changeof white rat liver (Rattus novergicus) due to induced with paracetamol toxic dose. This study used 24 male white rats, divided into four groups, negative control group (P0) given placebo, positive control group (P1) given paracetamol dose 250 mg / kg bw for 10 days, P2 given ant nest extract 250 mg / kg bw and paracetamol dose 250 mg / kg bw for 10 days, P3 given ants nest extract 250 mg / kg bw for seven days, then continued by giving paracetamol and ants nest extract with dose 250 mg / kg bw for ten days. After the treatment done, all the rats were dinecropsed. Liver organs were taken and processed for making histopathology preparations. Parameters examined included hemorrhage, congestion, degeneration and necrosis. The data obtained were analyzed statistically by using Kruskal Wallis test followed by Mann Whitney test. Mann Whitney test results for all categories of histopathologic changes in  hemorrhagic, congestion, degeneration, and necrosis between negative control group (P0) and positive control group (P1) were significantly different (P <0.05), between negative control (P0) with P2 and P3 there was no significant difference (P> 0,05). Afterward, between the positive control (P1) and P2 with P3 there was a significant difference (P <0.05). I can be concludedthat the administration of paracetamol dose 250 mg/kg bw for 10 days affects the histopathologic changes of white rat liver. The administration of ant nest plant extracts can reduce the side effects of toxic doses of paracetamol.

scholarly journals Pengaruh Kitosan Terhadap Jumlah Osteoklas dan Osteoblas Pada Tikus Galur Wistar Model Menopause

2017 ◽  
Vol 1 (2) ◽  
pp. 76-87
Author(s):  
Muhammad Nur
Keyword(s):  
Treatment Group ◽  
Total Sample ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Positive Control Group ◽  
Control Group ◽  
Control Group Design ◽  
White Rats ◽  
Significant Difference

Chitosan is one of the most abundant and dispersed polymers in nature. It has a rapid healing effect for tissues and has an effect on bone remodeling. The purpose of this study was to prove that giving chitosan influenced the decrease of osteoclast number and increasing number of osteoblasts in female mice of wistar strain of ovariectomy menopause model.This study used a posttest only control group design. The total sample of 30 rats divided into three groups was randomized, osteoporosis was made with the menopausal model which was performed ovariectomy in the positive control group and the treatment group then in the treatment group was given chitosan extract of single dose of 45 mg / day via Nasogastric Tube (NGT). K1 was a negative control group (without ovariectomy and without chitosan), K2 was a positive control group (with no chitosan ovariectomy), K3 was the treatment group (ovariectomy and chitosan 45 mg / day).The results showed significant differences in the number of osteoblasts between K1 and K3, K2 with K3, and no significant differences were found in the K1 group with K2. While the number of osteoclasts showed that there were significant differences in K1 group with K2 and no significant difference was found in K1 group with K3, K2 with K3.Conclusions in this study showed that 45 mg / day of chitosan doses could significantly increase osteoblast counts in menopause female white rats and decrease the number of osteoclasts performed by ovariectomy with chitosan administration.

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