Growth Characteristics and Asiaticoside Content, and Antioxidant Activities in Centella asiatica by Cultivation and Irrigation Methods

2020 ◽  
Vol 28 (4) ◽  
pp. 254-259
Author(s):  
Jang Nam Choi ◽  
Hee Jung Lee ◽  
Yun Ji Lee ◽  
Jin Tae Jeong ◽  
Jeong Hoon Lee ◽  
...  
Keyword(s):  
Antioxidant Activities ◽  
Centella Asiatica ◽  
Growth Characteristics ◽  
Irrigation Methods

scholarly journals Influence of selenium bio-fortification on nutritional compositions, bioactive compounds content and anti-oxidative properties of young ricegrass (Oryza sativa L.)

2017 ◽  
Vol 7 (3) ◽  
pp. 195 ◽  
Author(s):  
Rattanamanee Chomchan ◽  
Sunisa Siripongvutikorn ◽  
Panupong Puttarak ◽  
Rungtip Rattanapon
Keyword(s):  
Oryza Sativa ◽  
Bioactive Compounds ◽  
Functional Food ◽  
Sodium Selenite ◽  
Antioxidant Activities ◽  
Oryza Sativa L ◽  
Food Product ◽  
Growth Characteristics ◽  
Selenium Content

Background: Young ricegrass (Oryza sativa L.) can be introduced as one of functional food product since sprouts have been much interested in this era due to their high nutritive values. Bio-fortification of selenium is one strategy to enhance plant bioactivity. However, the level of selenium used is varied among species of plants, hence, the proper level needs to be explored.Objective: To investigate the influence of selenium bio-fortification on nutritional compositions, bioactive compounds content and anti-oxidative properties of young ricegrass.Methods: Sodium selenite ranging 0, 10, 20, 30 and 40 mg Se/L has been hydroponically bio-fortified into ricegrass then grown for 8 d and investigated the changes of growth characteristics, selenium content, accumulation of bioactive compounds and anti-oxidative properties.Results:  Results revealed that selenium bio-fortified exogenously increased the accumulation of selenium in ricegrass by 529% at 40 mg Se/L treatment without negatively changes in leaves biomass at the day of harvesting. However, root part weight slightly decreased when increased selenium level. Selenium at concentration of 10 and 20 mg Se/L can stimulate the production of phenolic compounds and antioxidant activities in young ricegrass as measured by DPPH, ABTS, FRAP and chelating assay. Conversely, higher level of selenium fortification reduced the accumulation of phenolics in ricegrass may due to pro-oxidant expression.Conclusion: Selenium bio-fortification can be used as a useful technique to improve quality of ricegrass plantation. 10 mg Se/L treatment was an ideal to trigger the synthesis of phenolics which exhibited high antioxidant activities. While, 40 mg Se/L treatment was ultimate for the production of Se plant foods.Keywords: Antioxidant activities; Bio-fortification; Ricegrass; Selenium

scholarly journals Insights Into Antioxidant Activities and Anti-Skin-Aging Potential of Callus Extract From Centella Asiatica (L.)

2021 ◽  
Author(s):  
Visarut Buranasudja ◽  
Dolly Rani ◽  
Ashwini Malla ◽  
Khwanlada Kobtrakul ◽  
Sornkanok Vimolmangkang
Keyword(s):  
Oxidative Stress ◽  
Callus Culture ◽  
Antioxidant Activities ◽  
Centella Asiatica ◽  
Skin Aging ◽  
Dermal Fibroblasts ◽  
Bioactive Metabolites ◽  
Chemical Components ◽  
Chemical Compositions ◽  
Scale Production

Abstract Formation of oxidative stress in dermal fibroblasts plays crucial roles in aging processes of skin. The use of phytochemicals that can promote capacity of fibroblasts to combat oxidative stress is an attractive strategy to prevent skin aging and promote skin beauty. Callus culture offers a powerful platform for sustainable, rapid and large-scale production of phytochemicals to serve extensive demands of pharmaceutical and cosmeceutical industries. Here, we demonstrated the application of callus culture of Centella asiatica to produce bioactive metabolites. The 50% ethanolic extract of callus culture has distinctive features of chemical compositions and biological profiles. Results from TLC and HPLC analysis showed that callus extract has unique chemical components, compared with those isolated from authentic plant. Moreover, callus extract possesses promising antioxidant and anti-skin-aging activities. Pre-treatment with callus extract attenuated H2O2-induced-cytotoxicity on human dermal fibroblasts. The results from RT-qPCR clearly suggested that the upregulation of cellular antioxidant enzymes appeared to be major contributor for the protective effects of callus extract against oxidative stress. Moreover, supplementation with callus extract inhibited induction of matrix metalloprotease-9 following H2O2 exposure, suggesting its potential anti-skin-aging activity. Our results demonstrate the potential utility of C. asiatica callus extract as anti-skin-aging agent in cosmeceutical preparations.

scholarly journals Comparison of the Antioxidant Activity of Malaysian Ginger (Zingiber officinale Roscoe) Extracts with that of Selected Natural Products and its Effect on the Viability of Myoblast Cells in Culture

2021 ◽  
Vol 50 (5) ◽  
pp. 1445-1456
Author(s):  
Nur Fatin Nabilah Mohd Sahardi ◽  
Faizul Jaafar ◽  
Siti Nor Asyikin Zakaria ◽  
Jen Kit Tan ◽  
Mariam Firdhaus Mad Nordin ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Natural Products ◽  
Moringa Oleifera ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Centella Asiatica ◽  
Butylated Hydroxytoluene ◽  
Therapeutic Effects ◽  
Antioxidant Power ◽  
Ficus Deltoidea

Ginger has been proven to possess various therapeutic effects, including antibacterial, anticancer, anti-inflammatory, and antioxidant effects. However, data on the comparison of ginger antioxidant activity with that of other natural products are still lacking. This study aimed to analyse and compare the antioxidant properties of two types of Malaysian ginger extracts (GE1 and GE2) with that of selected natural products. The antioxidant activities were measured by 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) and ferric reducing antioxidant power (FRAP) assays, while cell viability was determined by 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfonyl)-2H-tetrazolium (MTS) assay. The order of the DPPH scavenging activities was as follows: vitamin C > palm tocotrienol-rich fraction (TRF) > á-tocopherol > N-acetylcysteine (NAC) > Ficus deltoidea > butylated hydroxytoluene (BHT) > Centella asiatica > GE2 > GE1 > Moringa oleifera > Kelulut honey; the order of the mean FRAP value was as follows: NAC > á-tocopherol > BHT > TRF > Ficus deltoidea > Moringa oleifera > GE2 = GE1 > Centella asiatica > Kelulut honey. The viability assays showed that both ginger extracts significantly increased the percentage of viable cells (p < 0.05). In conclusion, neither of the ginger extracts was cytotoxic toward cells and both possessed comparable antioxidant properties, indicating their potential for ameliorating oxidative stress.

scholarly journals Interrelationship between secondary metabolites and antioxidant capacities of Centella asiatica using bivariate and multivariate correlation analyses

2021 ◽  
Vol 64 (1) ◽  
Author(s):  
Hyun Young Shin ◽  
Hoon Kim ◽  
Soontag Jung ◽  
Eun-Jin Jeong ◽  
Kyung-Haeng Lee ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Antioxidant Activities ◽  
Pearson Correlation ◽  
Principal Component ◽  
Centella Asiatica ◽  
Multivariate Correlation ◽  
Antioxidant Capacities ◽  
Whole Plant ◽  
Freeze Dried ◽  
Ethanol Extracts

AbstractCentella asiatica L. Urban (CA) is a valuable medicinal herb that contains various bioactive secondary metabolites. In the present study, the harvested CA was divided into whole plant and leaf parts, and were heated-air-dried or freeze-dried. The dried CA was extracted under various extraction conditions to obtain 16 extracts, and their antioxidant activities were examined. Twelve types of secondary metabolites (five polyphenolic acids, four triterpenoids, and three flavonoids) were quantified in each extract. Finally, the intercorrelations between secondary metabolites and antioxidant activities were analyzed through statistical analyses, such as Pearson correlation coefficient, scatter plotting, and principal component analysis. The antioxidant capacities of CA might be primarily influenced by major triterpenoids such as madecassoside and asiaticoside, which showed high content in the ethanol extracts of freeze-dried leaf parts. The present study provides a valuable groundwork for the development of optimal extraction processes for C. asiatica L. as an antioxidant material.

scholarly journals Antioxidant Activities of Centella asiatica Extract-loaded Bovine Serum Albumin Nanoparticles in Simulated Gastrointestinal System Study

2020 ◽  
Vol 141 ◽  
pp. 03001
Author(s):  
Kittiya Kesornbuakao ◽  
Patteera Chanapongpisan ◽  
Malinee Sriariyanun ◽  
Ir. Lindayani ◽  
Patchanee Yasurin
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Crude Extract ◽  
Bovine Serum ◽  
Antioxidant Activities ◽  
Centella Asiatica ◽  
Gastrointestinal System ◽  
Physical Parameters ◽  
Active Compounds ◽  
Albumin Nanoparticles

Bioavailability of active compounds extracted from herbs is generally limited to be adsorbed or expressed to target organisms due to several physical and chemical factors. Nanoparticle encapsulation techniques was developed to carry bioactive macromolecules of Centella asiatica (Buabok) in the form of C. asiatica Extract-loaded Bovine Serum Albumin Nanoparticles (CBNPs) to improve bioavailability. In this study, the antioxidant activities of CBNPs and C. asiatica crude extract were evaluated by using DPPH radical scavenging assay in the simulated gastrointestinal system, including mastication, stomach, duodenum, and ileum conditions to provide the environment which similar to in vivo system in terms of chemical and physical parameters. CBNPs were prepared by mixing of C. asiatica crude extract and BSA at different ratio of 1:2, 1:3, and 1:4. The results showed that the highest antioxidant activity of CBNP was observed when the ratio of crude extract and BSA at 1:2 was used at pH 2.0 or in the simulated stomach condition. The denaturation or unfolding of BSA in the simulated stomach occurred when pH was low could lead to the release of active compounds at certain area in gastrointestinal system.

Chromatographic Assessment of Polyphenolic Profile and Total Phenolic Content and Antioxidant Activity of Common Leafy Vegetables in Bangladesh

2020 ◽  
Vol 7 (1) ◽  
pp. 40-50
Author(s):  
Mohammad Khairul Alam ◽  
Rumana Tuli ◽  
Mohammad Sharif Khan ◽  
Abu Tareq Mohammad Abdullah ◽  
Maksuda Khatun ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Chronic Diseases ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Centella Asiatica ◽  
Leafy Vegetables ◽  
Total Phenolic ◽  
Polyphenolic Composition ◽  
Freeze Dried

Background: Polyphenolic compounds are known to provide health benefits and protect against degenerative chronic diseases. Utilization and identification of foods with a high content of these compounds are gaining greater attention nowadays. Objective: The present study reports the total phenolic content (TPC), polyphenolic composition and antioxidant activity (DPPH, FRAP and TEAC) of 10 commonly consumed leafy vegetables growing in Bangladesh. Materials and Methods: The samples were collected from different locations of Bangladesh and mixed together to ensure sample representativeness. Folin-Ciocalteu method was used for the analysis of TPC, and quantification of polyphenolic components was done by high-performance liquid chromatography (HPLC- DAD). Additionally, antioxidant activities of the selected vegetables were also analysed by utilizing DPPH, FRAP & TEAC. Results and Discussion: TPC ranged from 23.64 ± 1.20 to 45.59 ± 3.04 mg gallic acid equivalent (GAE)/g freeze-dried sample (fds). The polyphenolic spectrum ranged from 0.30 ± 0.02 to 647.42 ± 147.12 mg/100 g fds; quantity and spectrum of which varied in the vegetables. Among the studied vegetables, Centella asiatica contained the highest amount of TPC (45.59 ± 3.04 mg GAE/g fds) and also exhibited high antioxidant capacities, as documented by DPPH, FRAP and TEAC assays. Moreover, Principal component analysis (PCA) of investigated variables clearly separated Centella asiatica from other samples. Conclusion: Phenolic compounds being strong antioxidants reduce the risk of chronic diseases and the finding of this study would aware the people to take vegetables rich in phenolics. It would also fill up the data gap in the existing food composition table of Bangladesh.

scholarly journals AKTIVITAS ANTIOKSIDAN DAUN PEGAGAN (Centella asiatica L.Urban) DAN BUNGA KRISAN (Crhysanthemum sp) PADA TIGA VARIASI SUHU PENGERINGAN

2020 ◽  
Keyword(s):  
Antioxidant Activity ◽  
Moisture Content ◽  
Antioxidant Activities ◽  
Block Design ◽  
Centella Asiatica ◽  
Drying Process ◽  
Temperature Variations ◽  
Drying Temperature ◽  
Randomized Block Design ◽  
Positive Results

This research aims to determine the effect of dryer temperature variations on the antioxidant activity of gotu kola leaves and chrysanthemum flowers. The research method used was the experimental method using Randomized Block Design (RBD) with 6 types of treatments and 2 replications. The drying process is carried out until it reaches the moisture content according to SNI for dry tea which is a maximum of 8%. Drying is done using a food dehydrator with three temperature variations (50°C, 55°C and 60°C). The results show that the higher the drying temperature, the faster the standard moisture content is reached. The higher the drying temperature, the weaker antioxidant activity produced. The best antioxidant activity was obtained using the 50°C drying temperature of 36.1 ppm for gotu kola leaf simplicia. While the best antioxidant activity of simplicia of chrysanthemum flowers was obtained at 50°C at 137.99 ppm. The six samples showed positive results on the flavonoid test. Keywords: Antioxidant activities; chrysanthemum flower; gotu kola leaf

scholarly journals Comparison of Total Phenolic Contents and Antioxidant Activities of Centella asiatica Extracts Obtained by Three Extraction Techniques

2019 ◽  
Vol 6 (2) ◽  
pp. 42-49
Author(s):  
Wan Nurul Huda Wan Zainal ◽  
FARA RESTINA MUSAHIB ◽  
NABILAH SYAFIQAH ZULKEFLEE
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Centella Asiatica ◽  
Extraction Yield ◽  
Total Phenolic ◽  
Extraction Techniques ◽  
Phenolic Contents ◽  
Total Phenolic Contents

Through different extraction techniques, the potential of Centella asiatica as a natural source of antioxidant was investigated. The C. asiatica aqueous extracts were obtained via infusion, decoction and ultrasonic-assisted extraction (UAE) techniques. The effects of different extraction techniques were studied on the extraction yield, total phenolic contents and antioxidant activity. The total phenolic contents of the extracts and antioxidant activity were examined using the Folin-Ciocalteu’s reagent and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity assay, respectively. Results indicated that the UAE exhibited the highest extraction yield, highest total phenolic contents, as well as highest antioxidant activity. The yield of the extracts increased in the order of infusion < decoction < UAE which were 18.2 %, 23.6% and 25.4 %, respectively. All extraction techniques had a significant effect (p <0.05) on the total phenolic contents and antioxidant activity of C. asiatica extracts. The total phenolic contents was ranged from 3.42 ± 0.030 to 8.32 ± 0.105 mg GAE/g dry extract while the antioxidant activity was in the range of 75 to 86 %. This study confirms that C. asiatica has the potential to be a good resource for the future development of natural antioxidant. In addition, extraction via UAE can be an ideal technique to obtain phytochemical-rich extracts from medicinal plants.

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