scholarly journals A Study on the Assessment of Galectin-3 Expression in Colorectal Neoplasm and its Relationship with Tumour Stage, in Tertiary Care Hospital, Kolkata, India

2021 ◽  
Author(s):  
Nandini Bhaduri Bhattacharyya ◽  
Anadi Roy Chowdhury ◽  
Susmita Mukhopadhyay ◽  
. Sneha
Keyword(s):  
Colorectal Cancer ◽  
Colon Cancer ◽  
Colorectal Adenocarcinoma ◽  
Tertiary Care ◽  
Malignant Neoplasms ◽  
Care Hospital ◽  
Tumour Stage ◽  
Galectin 3 ◽  
Cancer Tissues ◽  
Lymphovascular Space Invasion

Introduction: Galectin-3 is a β-galactoside-binding lectin found in a considerable number of normal tissues and malignant neoplasms. It was found to be expressed in few thyroid tumours particularly follicular and papillary tumours. Lectins were found to be released in circulation and increased concentration was noted in colorectal cancers especially in metastatic colonic adenocarcinoma. Different types of galectins are expressed in normal colonic and rectal epithelium. Some types do increases in inflammation and cancers of these areas. It was seen that galectin-3 increases in colorectal tumourigenesis and it bears an important role in cancer progression and metastasis. Galectin-3 seems to have an important role in colorectal cancer. Some studies proved that galectin inhibitors could reduce tumour progression and metastasis and it may be a therapeutic target in metastatic colorectal adenocarcinoma. Aim: To evaluate colon cancer specimens received for biopsy, for galectin-3 expression and its relation with tumour stage, lymphovascular space invasion and tumour differentiation. Materials and Methods: The study was a cross-sectional observational study conducted in the Department of Pathology of RG Kar Medical College and Hospital from November 2018 to November 2019. It was an immunohistochemistry based assay performed to test the expression levels of galectin-3 in cancer tissues of 62 colorectal neoplasms with the help of galectin-3 primary antibody (mouse monoclonal antibody- clone 9C4). Statistical analysis was done using Statistical Package of Social Sciences (SPSS) version 19.0. Results: Out of 62 cases, 60 cases were colorectal adenocarcinoma and 2 cases were adenoma with age group between 40-75 years. Total 46 cases had cancer in caecum and ascending colon and rest were in recto-sigmoid colon. A 36/60 cases (60%) of cancer tissues were positive for galectin-3 expression. Strong association of lymphovascular space invasion (p=0.046) and depth of tumour (p=0.0078) with galectin positivity in colon carcinoma was noted. Conclusion: Evaluation of galectin-3 expression is helpful in the assessment of tumour staging and prognosis in colorectal cancer patients. It may have a therapeutic implication in the management of colon cancer in future.

scholarly journals Recurrence of sigmoid colon carcinoma in the retained urethra after cystectomy: A case report and review of the literature

2015 ◽  
Vol 87 (2) ◽  
pp. 167
Author(s):  
Yusuke Yagihashi ◽  
Yoshitaka Arakaki
Keyword(s):  
Colorectal Cancer ◽  
Colon Cancer ◽  
Sigmoid Colon ◽  
Colorectal Adenocarcinoma ◽  
Disease Free Survival ◽  
Sigmoid Colon Cancer ◽  
Review Of The Literature ◽  
Free Survival ◽  
Urethral Recurrence ◽  
Disease Free

Urethral recurrence arising from a primary colorectal adenocarcinoma is rare. Here, we report a case of urethral recurrence of sigmoid colon cancer, which developed after cysto-prostato-sigmoidectomy for sigmoid colon cancer invading the bladder. The patient underwent urethrectomy successfully and is currently tumor-free. Surgeons who follow patients with colorectal cancer invading the bladder should be aware of this case. The early detection of recurrence improves the chances for disease-free survival.

Colorectal cancer in young adults in a tertiary care hospital in Chhattisgarh, Raipur

2013 ◽  
Vol 50 (4) ◽  
pp. 337 ◽  
Author(s):  
N Hussain ◽  
R Gahine ◽  
J Mourya ◽  
V Sudarshan
Keyword(s):  
Colorectal Cancer ◽  
Young Adults ◽  
Tertiary Care Hospital ◽  
Tertiary Care ◽  
Care Hospital

scholarly journals Galectin-3 expression in colorectal cancer and its correlation with clinical pathological characteristics and prognosis

Open Medicine ◽  
2017 ◽  
Vol 12 (1) ◽  
pp. 226-230 ◽  
Author(s):  
Liu Tao ◽  
Li Jin ◽  
Li Dechun ◽  
Yang Hongqiang ◽  
Kou Changhua ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Protein Expression ◽  
Cancer Patients ◽  
Biological Marker ◽  
Progression Free Survival ◽  
Survival Times ◽  
Expression Levels ◽  
Galectin 3 ◽  
Colorectal Cancer Patients ◽  
Cancer Tissues

AbstractObjectiveTo explore the expression levels of galectin-3 in colorectal cancer and the association between galectin-3 and its clinical pathological parameters, as well as the prognosis of colorectal cancer patients.MethodsAn immunohistochemistry assay was used to test the expression levels of galectin-3 in cancer tissues of 61 colorectal cancer cases and in normal intestinal tissues adjacent to the cancer tissues of 23 cases. The associations between protein expression levels of galectin-3 and the clinicopathological features, such as age, sex, pathology type, lymphatic metastasis, and prognosis were also analyzed.ResultsThe positive rate of galectin-3 in cancer tissues was significantly higher than that of cancer-adjacent tissues: 62.5% (38/61) versus 13.0% (3/23) (P<0.05), respectively. Correlation was found between the protein expression of galectin-3 and the tumor size (P<0.05), as well as between the tumor differentiation (P<0.05) and Duke staging (P<0.05). The median progression-free survival times of patients with galectin-3 positive and negative expression were 19.2 and 35.1 months, respectively, with significant statistical difference (P<0.05).ConclusionGalectin-3 expression was correlated with the genesis and development of colorectal cancer and which could be used a biological marker for the prognosis of colorectal cancer patients.

scholarly journals Colon cancer presenting as pseudo-obstruction during pregnancy – A case report

2017 ◽  
Vol 10 (4) ◽  
pp. 198-200 ◽  
Author(s):  
Reeva Makhijani ◽  
Vicky H Bhagat ◽  
Mariam Fayek
Keyword(s):  
Colorectal Cancer ◽  
Colon Cancer ◽  
Colorectal Adenocarcinoma ◽  
Bowel Movement ◽  
Medical Oncology ◽  
Second Trimester ◽  
Total Abdominal Colectomy ◽  
Cancer In Pregnancy ◽  
History Of ◽  
In Pregnancy

The incidence of colorectal cancer in pregnancy is between 0.002 and 0.008%. Diagnosis is often delayed as symptoms of colorectal cancer can mimic as common complaints of pregnancy. We present the case of a 29-year-old with a history of chronic constipation who presented in the second trimester with abdominal pain, inability to tolerate anything orally and no bowel movement in more than three weeks. Non-contrast MRI at presentation failed to show an obstructing mass. Patient was treated conservatively for presumed pseudo-obstruction secondary to worsening constipation from chronic ondansetron use and pregnancy. After four days without clinical improvement, she had a colonoscopy that revealed a completely obstructing sigmoid mass, which biopsies confirmed was a primary colorectal adenocarcinoma. The patient underwent a total abdominal colectomy. She was referred to medical oncology and began adjuvant chemotherapy consisting of 5-fluorouracil, leucovorin and oxaloplatin four weeks post-operatively.

scholarly journals MicroRNA silencing of CNN1 and CNN2 protein family members regulates biology processes in colorectal cancer by targeting the p53 signal pathway

2020 ◽  
Author(s):  
Fuda Huang ◽  
Mingwei Wei ◽  
Anmin Wang ◽  
Ya Zhang ◽  
Zebang Qin ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Cell Cycle ◽  
Colon Cancer ◽  
Cell Lines ◽  
Cancer Cell ◽  
Cancer Cell Lines ◽  
Colon Cancer Cell ◽  
Expression Levels ◽  
Colon Cancer Cell Lines ◽  
Cancer Tissues

Abstract BackgroundCalponin was first defined as a striated muscle troponin T-like protein that binds actin thin filaments to regulate smooth muscle contraction. There are few studies of CNN1 and CNN2 in colorectal cancer, and the roles these two genes play in colorectal cancer cell lines and the mechanisms by which they act are unknown.MethodsWe used immunohistochemistry to identify expression of the two genes in the cancer tissues. RT-PCR was used to measure expression levels of microRNA. W performed western blots to measure changes in signaling pathways in the context of expression interference.Meanwhile, the same method was used to measure binding relationship between the two genes and key pathway proteins. To determine the relationship between microRNA and gene mRNA, we used the reporter gene method. We used the chi-square and t-test methods to analyze the significance and correlations of the data.Results and conclusionsExpression levels of CNN1 were lower in colon cancer tissues than in normal mucosal tissues. After downregulating CNN1, the cell cycle in colon cancer cell lines progressed quickly, and the expression of related pathway proteins also increased. Expression levels of CNN2 were higher in colon cancer tissues, and its downregulation significantly inhibited cell cycle progression in colon cancer cell lines. We confirmed correlations between the expression of microRNA and CNN2 using data analysis.Bars indicate ± standard errors.*p < 0.05; **p < 0.01 compared with the control. The inhibition of the expression of CNN2 mRNA using microRNA was confirmed using western blot. The combination of the two at the mechanism level was also demonstrated using the reporter gene method.

scholarly journals The expression and Clinical Significance of miRNA-135a and Bach1 in colorectal cancer

2021 ◽  
Author(s):  
Yang zhi Jiang ◽  
Qing Guo Tao ◽  
fei yan Zhu
Keyword(s):  
Colorectal Cancer ◽  
Colon Cancer ◽  
Clinical Information ◽  
Control Group ◽  
Tumor Control ◽  
Cancer Tissue ◽  
Expression Levels ◽  
Real Time Quantitative Pcr ◽  
Normal Tissues ◽  
Cancer Tissues

BACKGROUND AIM To explore the correlation between the expression of miRNA-135a and Bach1 in colorectal cancer tissue and the patient's clinical information.  Methods   60 patients with colorectal carcinoma were treated as a control group. Real-time quantitative PCR assays and immunohistochemistry method were performed to detect the expression of miRNA-135a and Bach1 in 60 colorectal carcinomas and adjacent normal tissues, and the clinical and pathological classifications had also been investigated. The SPSS 19.00 software was used. All data represented mean±SD of three independent experiments. P&lt;0.05 was considered statistically significant. Results  miRNA-135a expression levels increased significantly in the colon cancer tissues compared with the non-tumor control tissues(P&lt;0.01). miRNA-135a expression levels were higher in stage III/IV than in stage I/II colon cancer patients. The expression level of Bach1 in colorectal cancer was significantly lower(P&lt;0.01). Bach1 and miRNA-135a were negatively correlated.  Conclusions:  The levels of miRNA-135a and Bach1 were opposite, the over-expression of miRNA-135a might downregulated the expression of Bach1, which might be involved in the pathogenesis of colorectal cancer.

scholarly journals A study on evaluation of the role of fine-needle aspiration cytology in the etiology of lymphadenopathy in the rural population attending tertiary care hospital

2020 ◽  
Vol 6 (7) ◽  
pp. 425-429
Author(s):  
Dr. C. Sitalata ◽  
◽  
Dr. K. Kalyan ◽  
Keyword(s):  
Lymph Nodes ◽  
Tertiary Care ◽  
Age Groups ◽  
Needle Aspiration ◽  
Metastatic Carcinoma ◽  
Malignant Neoplasms ◽  
Care Hospital ◽  
Tertiary Care Centre ◽  
Lymphoreticular Tissue ◽  
Reactive Hyperplasia

Introduction: Lymph nodes are a site for organized collections of lymphoreticular tissue and arepink-gray bean-shaped encapsulated organs. Lymph nodes are among the commonly aspiratedorgans for diagnostic purposes. Lymphadenopathy is of great clinical significance as underlyingdiseases may range from a treatable infectious etiology to malignant neoplasms Fine needleaspiration cytology (FNAC) is a reliable as well as an inexpensive method used to diagnoselymphadenopathy of various sites. Aims: To evaluate the usefulness of FNAC as a diagnostic tool inthe etiological causes of lymphadenopathy and to study the cytomorphological features associatedwith various lymphadenopathies. Materials and Methods: The present prospective study wascarried out in the Department of Pathology at Maharajah's Institute of Medical Sciences,Vizianagaram, a Tertiary Care Centre. A total of 210 patients of all age groups underwent FNAC ofenlarged lymph nodes during this study period. Results: FNAC diagnosis was found to be as follows:tubercular lymphadenitis in 98 cases (46.67%) followed by reactive hyperplasia in 35 cases(16.67%), metastatic carcinoma 29 (13.8%), granulomatous lymphadenitis 27 (12.85%), non-specific lymphadenitis 8 (3.8%), acute suppurative lymphadenitis 7 (3.33%) and lymphoma 4 (1.8).Conclusion: FNAC of lymph nodes is an excellent first-line investigation to determine the nature ofthe lesion. It is quick, safe, minimally invasive, and reliable and is readily accepted by the patient.

scholarly journals Clinicopathological Study of 50 Cases Colorectal Carcinoma At Tertiary Care Hospital

2013 ◽  
Vol 4 (2) ◽  
pp. 53-56
Author(s):  
Md Abdul Quddus ◽  
Md Alimunzzaman ◽  
Md Zilluar Rahman ◽  
Md Kausar Alam
Keyword(s):  
Colorectal Cancer ◽  
Colorectal Carcinoma ◽  
Rectal Carcinoma ◽  
Colonic Cancer ◽  
Tumour Size ◽  
Tertiary Care ◽  
Bowel Habit ◽  
Care Hospital ◽  
College Hospital ◽  
Serum Cea

Background: Colorectal carcinoma is a major causes of mortality and morbidity worldwide. Objective: The purpose of the present study was to consider the socio-demographic status and serum CEA level as diagnostic tool for advanced stages of colorectal cancer. Methodology: This analytical cross-sectional study was conducted in the Department of Surgery at Shaheed Ziaur Rahman Medical College Hospital, Bogra during the period of January 2010 to December 2011. Patients age ranged from 15 years to 75 years and above were included in this study. All efforts and available modern technologies including ultrasonography (USG), CT scan, endo-anal USG, chest X-ray were done to detect the primary site and metastasis of colorectal cancer. Result: The most frequent colorectal cancer was detected in 35-44 years age group with 60.0% male and 40.0% female patients. A total number of 41(82%) cases had been suffering from rectal carcinoma and 9(18%) with colonic cancer. Abdominal pain, anorexia, altered bowel habit, per rectal bleeding and abdominal lump were the cardinal features in clinical presentation. This study showed that serum CEA level was raised in 3(11.11%) cases with tumour size 2-5 cm and 21(91.30%) cases with tumor size >5cm. It was observed that serum CEA level was raised in 100% cases of Dukes stage 'D', 92.31% cases of stage 'C' and 30.56% cases of stage 'B' colorectal cancer. The serum CEA level in relation with tumour size and stage has been proved highly significant (p<0.001). Conclusion: Rectal carcinoma is found common than colonic cancer and level of serum CEA is directly related to the cancer stage. DOI: http://dx.doi.org/10.3329/jssmc.v4i2.14413 J Shaheed Suhrawardy Med Coll, 2012;4(2):53-56

scholarly journals Comparison of Treatment Outcomes between Right- and Left-sided Colorectal Adenocarcinoma after Adjuvant Chemotherapy

2021 ◽  
Vol 6 (4) ◽  
pp. 389-395
Author(s):  
Dean Marvin Potato Pizarro ◽  
Carlos Dy ◽  
Mel Valerie` Cruz- Ordinario ◽  
Cyrielle Marie Atutubo
Keyword(s):  
Colorectal Cancer ◽  
Colon Cancer ◽  
Adjuvant Chemotherapy ◽  
Colorectal Adenocarcinoma ◽  
Locally Advanced ◽  
Survival Outcomes ◽  
Metastatic Setting ◽  
Significant Difference ◽  
Disease Free ◽  
Tumor Laterality

Objective: The survival outcomes of patients with metastatic colorectal adenocarcinoma based on tumor laterality has already been established based on large-scale retrospective studies. As for the non-metastatic disease, the data is much more conflicting. Local data in this population is also scarce. In this study, we determined the difference in survival of right-sided versus left-sided colorectal cancer patients after surgery and adjuvant chemotherapy. Patients and Methods: This retrospective study analyzed a total of 124 patients who were diagnosed with early to locally advanced (stage I to IVA) colorectal adenocarcinoma and underwent definitive surgery and adjuvant systemic treatment per physician’s preference. The patients were stratified according to the primary tumor laterality: Right-Sided Colon cancer (RCC) and Left-sided Colon cancer (LCC). The primary outcome being investigated is the disease-free survival (DFS) at 3 years and 5 years. Secondary outcomes are overall survival (OS) at 3 years and 5 years, and site of disease recurrence across laterality.Results: The findings did not show a significant difference in the 3-year and 5-year DFS between RCC and LCC after surgery and adjuvant chemotherapy (3-year DFS: 50.0% in the RCC group and 54.3% in the LCC group; P=.671; 5-year DFS: 36.7% in the RCC group and 36.2% in the LCC group, P=.474). The OS difference was also not significant at 3 years and 5 years (3-year OS: 68.8% in the RCC group and 73.9% in the LCC group, P=.572; 5-year OS: 31.3% in the RCC group and 26.1% in the LCC group, P=.474). The overall recurrence rate was not significantly different in both groups (65.6% in the RCC group and 66.3% in the LCC group; P=.944). Majority of the recurrences were at a distant site in both groups (61.1% in the RCC group and 69.6% in the LCC group; P=.501) and the most common were in the lungs, and liver. Conclusion: The survival outcome of non-metastatic colorectal adenocarcinoma was not significantly different between RCC and LCC after surgery and adjuvant chemotherapy. The outcome was also similar after stratification of the population into early and locally advanced disease. More diverse clinical characteristics other than tumor location may be prognostic of disease-free and survival outcomes in the non-metastatic setting of colorectal cancer. Further studies with a larger sample size are needed to validate the correlation of survival to the specific stage and anatomic location.

Early detection of lower GI tract tumors by dedicated assessment of the colon on routine computed tomography (CT) imaging: An observational study.

2019 ◽  
Vol 37 (4_suppl) ◽  
pp. 510-510
Author(s):  
John Chang ◽  
Russell Pluhm ◽  
Ashley Lutrick ◽  
Jessica Guerra ◽  
Phillip Koo ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Early Detection ◽  
Lymph Nodes ◽  
Tertiary Care ◽  
Transitional Cell ◽  
Wall Thickening ◽  
Average Risk ◽  
Care Hospital ◽  
Early Features

510 Background: We have previously reported that up to 48% of the early features of colorectal cancer (subtle wall thickening, pericolonic stranding, and small lymph nodes in the draining nodal station) were not identified on the original CT abdomen and pelvis (CTAP) reports. This resulted in a 36% decrease in five-year survival based on historical data. In this report, we assessed whether dedicated assessment of the colon on routine CT scans could lead to early detection of colorectal cancer. Methods: 210 CTAPs over a three-month period were screened from the emergency room records at a tertiary care hospital. 194 scans met eligibility. Exclusion criteria included: cases known to the evaluating radiologist and age ≤ 19 or > 89 years. No study was excluded for suboptimal image quality. The original report was reviewed for abnormalities involving the colon, mesentery and bowel and was recorded. A blinded evaluation of the eligible case was then performed by a board-certified radiologist with attention specifically to the colon and the mesentery for the suspicious early features of CRC. The concordance and discordance was then tabulated. Discordant findings were re-evaluated to determine if the discordance was true. Results: 72/194 patients were male, median age 44.5 years (range 20 - 89). 55/194 patients (29.1%) included in the study were noted to have suspicious features. 26 had abnormal lymph nodes, 24 had abnormal colonic wall thickening and 16 had pericolonic stranding and/or wall edema. 45/55 studies were truly discordant from the original interpretation. These included one missed colorectal cancer (confirmed), one likely small bowel neuroendocrine tumor (no follow up), and one likely transitional cell carcinoma of the right renal pelvis (no follow up). Conclusions: Dedicated search of the colon and mesentery on CTAP can identify subtle findings, although their true relevance is being evaluated in a larger future study. Our observational data does indicate that there maybe a potential role for a focused evaluation of the colon and mesentery on routine CTAP in an attempt to potentially increase the rate of cancer detection especially in younger low-average risk patients.

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