scholarly journals Toxicity of Crude Fruit Endocarp Extract of Calabash (Lagenaria siceraria) on African Catfish (Clarias gariepinus) Juveniles

2020 ◽  
pp. 31-46
Author(s):  
J. O. Ayorinde ◽  
B. S. Audu ◽  
T. O. Ogundeko ◽  
A. I. Ujah
Keyword(s):  
Aqueous Extract ◽  
Clarias Gariepinus ◽  
Reproductive Organs ◽  
The Body ◽  
Total Alkalinity ◽  
African Catfish ◽  
Aquatic Life ◽  
Experimental Media ◽  
Secondary Lamellae ◽  
Histopathological Studies

Background: Illicit disposal of scraped out endocarp of bottle gourds as part of processing activity for domestic use into the aquatic environment pose a big threat to the aquatic ecosystem. This study investigated the toxicity effects of crude fruit endocarp extracts of L. siceraria on C. gariepinus juveniles. Methodology: 120 mixed sex of C. gariepinus juveniles of mean weight and length (19.59 ±0.42 g; 14.6 ±0.80 cm) respectively were investigated. The fish were divided into 6 groups of 10 fish in each aquarium containing 5, 20, 35, 50 and 65 mg/L concentration of aqueous extract of L. siceraria and 0.00 mg/L (control) respectively with replicates for Four (4) days for acute toxicity analysis. The animals were sacrificed and blood samples collected for biochemical and isolation of the gills and liver for histopathological studies. Results: Experimental media pH, free carbon (iv) oxide, total alkalinity contents increased while dissolved oxygen decreased significantly (P<0.05) with increase in concentration in the acute bioassay. Temperature did not differ significantly (P>0.05) in all the groups thus, did not affect fish survival. Furthermore, AST, ALT and LDH profiles in blood serum of fish exposed to acute concentrations exhibited a significant increase (P<0.05) with increase in concentration. The exposed gills revealed progressive striking histological alterations viz. thickening and shortening of secondary lamellae, degeneration of connective tissue and complete loss of secondary lamella while the liver showed progressive histo-architectural distortions such as hepatic hypertrophy, cellular degeneration (necrosis), haemorrhage and intracellular infiltration with increase in concentration of the L. siceraria extract. Conclusion: Aqueous extract of L. siceraria had adverse effect on juveniles of C. gariepinus, as evident in the negative modification of the body physiology via biochemical and histological investigations, thus toxic to the aquatic life. Further investigations on other vital organs - kidney, heart, GIT and the reproductive organs are recommended.

EFEKTIVITAS ANTIBAKTERI BAWANG PUTIH (Allium sativum L) SEBAGAI PENGAWET ALAMI PADA IKAN LELE DUMBO (Clarias gariephinus) SEGAR

2019 ◽  
Vol 14 (1) ◽  
pp. 26
Author(s):  
Dyah Anggraeni ◽  
Nurlela Nurlela
Keyword(s):  
Room Temperature ◽  
Allium Sativum ◽  
Research Result ◽  
Clarias Gariepinus ◽  
Storage Period ◽  
The Body ◽  
Garlic Extract ◽  
African Catfish ◽  
Natural Preservatives ◽  
Colony Counter

Background: Natural preservatives are compounds produced by natural ingredients that can suppress bacterial growth and development. Natural preservatives are carried out because most of the preservatives circulating are chemicals and unsafe for the body. One of the natural preservatives is by using garlic extract (Allium sativum L).  Objective: This study is aimed to determine the effectiveness of the antibacterial garlic (Allium sativum L) as a natural preservative in fresh African catfish (Clarias gariepinus).  Method: This research used the Pour Plate iroculation method. African catfish (Clarias gariepinus) which is soaked with garlic (Allium sativum L) with a concentration of 7%, 14% and 21% for 30 minutes, then the fish will be kept at room temperature with a storage period of 24 hours and 48 hours and calculated growth in bacterial numbers with the Colony counter.  Result: Based on the research result, it was found that garlic extract (Allium sativum L) can obstruct the effectiveness of antibacterial in African catfish (Clarias gariepinus) at a concentration of 14%.

scholarly journals Dietary canitine maintains energy reserves and delays fatigue of exercised african catfish (Clarias gariepinus) fed high fat diets

2005 ◽  
Vol 62 (3) ◽  
pp. 208-213 ◽  
Author(s):  
Rodrigo Ozório ◽  
Vincent Van Ginneken ◽  
Guido van den Thillart ◽  
Martin Verstegen ◽  
Johan Verreth
Keyword(s):  
Lipid Level ◽  
Juvenile Fish ◽  
Clarias Gariepinus ◽  
Energy Charge ◽  
The Body ◽  
African Catfish ◽  
Energy Reserves ◽  
High Fat ◽  
High Fat Diets ◽  
Fat Diets

Lipids, together with proteins, are traditionally considered as primary fuels during aerobic swimming. The effects of dietary fat and carnitine supplements and exercise on the energy metabolism of juvenile fish were investigated. One hundred African catfish (Clarias gariepinus) were fed four isonitrogenous diets containing a fat level of 100 or 190 g kg-1 diet and one of the two levels of carnitine (15 and 1000 mg kg-1). Fish grew from 61 to 162 g in 10 wk. Thereafter, 6 fish per group swam vigorously for 3 h and the results were compared with unexercised groups. Fish receiving 1,000 mg carnitine accumulated 2- to 3-fold more carnitine than fish receiving 15 mg carnitine. Plasma acyl-carnitine level was affected by an interaction between dietary treatment and exercise (P < 0.05). Adenosine triphosphate and phosphocreatine concentrations were higher in the white muscle (WM) of exercised fish fed the high-carnitine supplements, compared with the low-carnitine fed fish (P < 0.05). Adenilate energy charge indexes were higher and ammonia concentrations were lower in WM of fish fed high-carnitine and high-fat diets. Dietary carnitine supplements may be needed in growing fish when dietary lipid level is high. In that case extra dietary carnitine can maintain the body energy reserves at adequate level when fish is exposed to a short-term, exhaustive exercise, a physiologic stress common both in nature and in intensive aquaculture systems.

scholarly journals Testicular Morphology and Sperm Motility in Cultured African Catfish (Clarias gariepinus) at Different Stages of Development

2016 ◽  
Vol 8 (3) ◽  
pp. 281-285 ◽  
Author(s):  
Chidozie Nwabuisi OKOYE ◽  
Udensi Maduabuchi IGWEBUIKE ◽  
Anietie Francis UDOUMOH ◽  
Chinadindu Tochukwu OKEREKE
Keyword(s):  
Sperm Motility ◽  
Clarias Gariepinus ◽  
Body Cavity ◽  
The Body ◽  
Sperm Cells ◽  
African Catfish ◽  
Testicular Weight ◽  
Testicular Morphology ◽  
The Mean ◽  
Somatic Index

Testicular morphology and sperm motility were evaluated in cultured Clarias gariepinus (n = 25) purposively assigned to five groups according to their age. The results showed that the testes were paired, elongated, dorso-ventrally flattened structures, situated in the caudal aspects of the body cavity. The mean length of both right and left testes increased linearly with age, being significantly (p < 0.05) higher at 6 months than at 4 and 5 months of age, and also significantly (p < 0.05) higher at 8 months than at 6 months of age, while the mean weight and organo-somatic index of the catfish testes increased linearly until 6 months of age, after which no significant (p > 0.05) increase in the testicular weight and organo-somatic index was observed. Unidirectional progressive movement of spermatozoa was detected in the milt of C. gariepinus at 6, 7 and 8 months of age, but sperm cells were non-motile at 4 and 5 months of age. Histological sections showed seminiferous lobules, whose germinal epithelia were characterized by many cysts enclosing clones of sperm cells. Each cyst enclosed a clone of sperm cells at an identical stage of spermatogenesis. Spermatids and spermatozoa were present in the lumen of the seminiferous lobule. The obtained results indicate that the morphology of the testes of C. gariepinus is similar to the testes of members of the order Siluriformes, but sexual maturity and production of motile spermatozoa may be achieved at 6 months of age in the African catfish.

scholarly journals The application of [D-Tle6,ProNHEt9]mGnRH (Lecirelin) with the dopaminergic inhibitor metoclopramide to stimulate ovulation in African catfish (Clarias gariepinus)

2011 ◽  
Vol 49 (No. 7) ◽  
pp. 297-306 ◽  
Author(s):  
E. Brzuska ◽  
J. Kouřil ◽  
J. Adamek ◽  
Z. Stupka ◽  
V. Bekh
Keyword(s):  
Body Weight ◽  
Female Body ◽  
Clarias Gariepinus ◽  
The Body ◽  
African Catfish ◽  
Average Body ◽  
Stimulation Of ◽  
Average Body Weight ◽  
Pituitary Homogenate

The results of reproduction were tested in females of the African catfish (Clarias gariepinus Burchell 1822) after stimulation of ovulation with carp pituitary (4 mg/kg body weight) or with Lecirelin (15 &mu;g/kg) and metoclopramide (10 mg/kg). After administering the synthetic substance eggs were obtained from all females while in the group treated with pituitary homogenate 7 out of 8 hypophysed females spawned. The applied spawning agent did not significantly influence the weight of eggs expressed in grams, but in the case of females treated with carp pituitary homogenate a significantly higher weight of eggs expressed as the percentage of body weight of fish was recorded. The applied stimulators of ovulation did not affect any trait reflecting the quality of eggs. Females used as an experimental material belonged to two categories in respect of body weight: lighter females with average body weight of 2.63 &plusmn; 0.36 kg and heavier females with average body weight of 3.91 &plusmn; 0.48 kg. It was proved that the weight of eggs expressed either in grams or as a percentage of a female&rsquo;s weight was significantly related to the body weight of a female (P &le; 0.01 and P &le; 0.05, respectively), as well as the percentage of fertilised eggs and the percentage of living embryos after 28 hours of incubation (P &le; 0.05 and P &le; 0.05, respectively). The interaction between the stimulator of ovulation and the female body weight was significant only for traits reflecting the weight of obtained eggs (P &le; 0.05 and P &le; 0.01). &nbsp;

Effect of Clarias gariepinus cultured in contaminated water on rats

2009 ◽  
Vol 28 (12) ◽  
pp. 759-764
Author(s):  
Oyeyemi Adeyemi ◽  
Olalekan Adeyemi ◽  
Chris C Osubor
Keyword(s):  
Enzyme Activity ◽  
Clarias Gariepinus ◽  
Elevated Serum ◽  
Liver Weight ◽  
The Body ◽  
Control Group ◽  
Albino Rats ◽  
Enzyme Assays ◽  
African Catfish ◽  
Wistar Strain

In this study, African Catfish (Clarias gariepinus) were cultured in water contaminated with phthalate, benzene and cyclohexane (10 μg/mL), respectively, over a period of 65 days. They were, thereafter, used as protein source to formulate feed for albino rats (Wistar strain) for 28 days. The weights of the body and selected tissues of the rats were monitored and standard enzyme assays were conducted for some enzymes in the serum. Results showed that rats in cyclohexane group gained 58% whereas the control group gained 46% of body weight. Liver weight (absolute and relative) of test rats were found to be significantly lower than that of control (p < .05). Enzyme activity of serum of test rats was found to be significantly higher than that of control (p < .05). Experimental evidence suggests leakage of enzymes from the liver to the serum, thus the elevated serum enzyme activity and that the contaminants may damage the liver.

scholarly journals Histopathological changes in gills and liver of Clarias gariepinus fingerlings exposed to acute concentrations of dry cell battery

2021 ◽  
Vol 19 (1) ◽  
pp. 26-33
Author(s):  
B.S. Audu ◽  
M. Damshit ◽  
J.O. Omirinde ◽  
I.A. Wakawa ◽  
Y. Sulaiman ◽  
...  
Keyword(s):  
Acute Toxicity ◽  
Clarias Gariepinus ◽  
Water Soluble ◽  
African Catfish ◽  
Histopathological Changes ◽  
Secondary Lamellae ◽  
Freshwater Habitats ◽  
Indiscriminate Disposal ◽  
Dry Cell ◽  
Soluble Fractions

Waste dry cell batteries are frequently improperly disposed and subsequently washed into water bodies-- causing deleterious effects on fish particularly Clarias gariepinus which inhabits diverse freshwater habitats. Acute toxicity of water-soluble fractions of waste dry cell batteries was investigated on C. gariepinus fingerlings under laboratory conditions in 96 hours. Ten (10) C. gariepinus fingerlings were exposed to acute concentrations (0.31, 0.63, 1.25, 2.50, and 5.00 g/L) of waste dry cell batteries and a control (0.00 g/L), each duplicate replicated. Histopathological alterations  evident in the gills were lamellar fusion, hyperplasia, inter-lamella space occlusion, hypertrophy and erosion of secondary lamellae. The liver showed nuclear and hepatocytes degeneration, vacuolation and portal congestion. Acute concentrations of water-soluble fractions of waste dry cell batteries caused significant (P<0.05) changes in the histomorphology of the gills and liver of C. gariepinus fingerlings, therefore indiscriminate disposal of waste dry cell batteries around riparian ecosystem should be safeguarded to reduce the declining diversity and abundance of freshwater fish species. Keywords: African catfish, 96 hr.LC50, Fingerlings, Histopathology Zinc-carbon battery

Effects of Acute Toxicity of Chlorpyrifos (EC 50%) and Associated Histological Alterations in Gills, Liver and Kidney of Mozambique Tilapia, Oreochromis mossambicus (Peters, 1852)

2020 ◽  
Author(s):  
N. Jayakumar ◽  
A. Subburaj ◽  
P. Jawahar ◽  
A. Srinivasan ◽  
B. Ahilan
Keyword(s):  
Acute Toxicity ◽  
Oreochromis Mossambicus ◽  
The Body ◽  
Dependent Manner ◽  
Histopathological Changes ◽  
Aquatic Life ◽  
Histological Changes ◽  
Mozambique Tilapia ◽  
Secondary Lamellae ◽  
Liver And Kidney

Background: The pesticides are known to adversely affect the quality of water and create hazards for aquatic life that results in severe damage to non-target freshwater organisms including fish. Among them, the organophosphorus pesticide, chlorpyrifos (CPF) is one of the most commonly used pesticides for controlling various kinds of pests in agriculture. Pesticides after entering in to the body of fish bring about histopathological and biochemical changes in different target and non-target organs. Hence, the present study aimed to investigate the toxicity effects of Chlorpyrifos (CPF) and associated histopathological changes in the gill, liver and kidney of the Mozambique Tilapia, Oreochromis mossambicus under the acute toxicity concentrations. Methods: Static bioassay was carried out for Chlorpyrifos with Oreochromis mossambicus as test animal for a period of 96 hrs as per standard methods and LC50 values were calculated through Probit analysis. The fishes were exposed to five acute concentrations (0.033, 0.066, 0.132, 0.264 and 0.528 ppb). The gill, liver and kidney tissues were collected from the fishes exposed to the pesticide and standard histology protocol was followed to investigate the histopathological changes. Result: The histological changes observed in the gill included lamellar aneurysm, curling of secondary lamellae, shortening of the secondary lamellae, hypertrophy of epithelial cell, fusion of secondary lamellae, deformation of the cartilage core, blood congestion, collapsed secondary lamellae, excessive mucus secretion, disorganization of the secondary lamellae, haemorrhage at primary lamellae, necrosis, haemorrhage at secondary lamellae. The most common histopathological changes in the liver were characterized by cellular necrosis, degeneration of hepatocytes, nuclear degeneration, fat deposition, rupture of nucleus, hypertrophied hepatocytes, blood congestion, blood sinusoids, cellular hypertrophy, increased pycnotic nucleus, cirrhosis and hemosiderosis. Further, histological changes like appearance of dilated lumen, hypertrophied epithelial cells, severe haemorrhage, blood congestion, melanomacrophage aggregation, narrowing of lumen, degenerated tubule, degenerated glomerulus, shrunken glomerulus and distended glomerulus were observed in the kidney. Thus, it is evident from the present study that Chlorpyrifos can be a potential toxicant affecting the fishes at tissue level on dose and time dependent manner which are expected to affect the other physiological processes in the long run.

scholarly journals Effectiveness of the Use of the Adaptogen Trekrezan in the Cultivation of African Catfish

2021 ◽  
Vol 37 ◽  
pp. 00176
Author(s):  
Elena Spirina ◽  
Elena Romanova ◽  
Ludmila Shadyeva ◽  
Vasily Romanov
Keyword(s):  
Physiological State ◽  
Clarias Gariepinus ◽  
The Body ◽  
Planting Density ◽  
Adaptive Plasticity ◽  
Histological Structure ◽  
African Catfish ◽  
Waste Products ◽  
Closed Water ◽  
Cellular And Humoral Immunity

Growing fish in closed water supply installations with a high planting density leads to accumulation of waste products, turning the habitat into toxic. Growing fish in such an environment leads to stress and increases the stress on their liver, which provides the body with detoxification of metabolites. To reduce stress, adaptogens are used, which increase endurance and survival, increase adaptive plasticity, and strengthen the immunity of fish. We used an adaptogen called Trekrezan. The work aim was to study the morphofunctional changes in liver and its microarchitectonics in African catfish grown using Trekrezan. The analysis of morphofunctional changes in liver allows us to judge the general physiological state of fish body. The liver index of African catfish grown without the use of Trekrezan is significantly higher in both males and females, compared to African catfish grown with Trekrezan, which indicates an intensification of metabolism. Analysis of histological structure of African catfish liver (Clarias gariepinus), grown without the use of the Ttrekrezan, revealed abnormalities of the parenchymal layer of liver with signs of fatty dystrophy, signs of necrotic changes in liver cells, hemorrhages in vascular region, a violation of polarity in the structure of hepatocytes. In species raised in an environment with Trekrezan, these abnormalities are absent, since Trekrezan, due to the activation of cellular and humoral immunity, leads to decrease in intoxication, provides strengthening of immunity, and increases the body's resistance to unfavorable factors of medium.

scholarly journals Female maturation and rematuration acceleration of Mutiara strain catfish (Clarias gariepinus) using combination of oocyte developer hormone and astaxanthin addition diet

2019 ◽  
Vol 18 (1) ◽  
pp. 23-32
Author(s):  
Fatahillah Maulana Jufri ◽  
Agus Oman Sudrajat ◽  
Mia Setiawati
Keyword(s):  
Clarias Gariepinus ◽  
Reproductive Organs ◽  
African Catfish ◽  
North African ◽  
Environmental Signals ◽  
Gonadal Maturation ◽  
Gonadal Maturity ◽  
Research Designs ◽  
Broodstock Diet ◽  
Female Maturation

ABSTRACT Reproductive design for gonadal maturation process mostly related with some factors such as environmental signals, reproductive organs, hormonal and nutrition. This research was conducted on female Mutiara strain of North African Catfish, Clarias gariepinus by combining two kinds of materials administered to broodstock diet, namely oocyte developer (Oodev) which contains of PMSG hormone and antidopamin, and astaxanthin carotenoid. Research designs were divided into C (Control), A50 (astaxanthin 50 mg/kg feed), A100 (astaxanthin 100 mg/kg feed), Od0.5 (Oodev 0.5 mL/kg fish for two weeks), Od1 (1 mL/kg fish for 2 weeks), Od0.5A50 (combined Od0.5 with A50), Od1A50 (combined Od1 with A50), Od0.5A100 (combined Od0.5 with A100), and Od1A100 (combined Od1 with A100). This research was performed during twelve weeks of feeding. The Od1A100 treatment showed the best reproduction performance result compared to other treatment with highest hepatosomatic (HSI) and gonadosomatic (HSI) indexes (P<0.05), also fastest increase in egg diameters (P<0.05), shorter rematuration periods and highest proportion of mature broodstock. These results indicated that Oodev and astaxanthin could accelerate gonadal maturity in female broodstock of Mutiara catfish.Keywords: Broodstock, hormonal, reproduction, oocyte developer, astaxanthin  ABSTRAK Rekayasa reproduksi untuk proses pematangan gonad sebagian besar terkait dengan beberapa faktor seperti sinyal lingkungan, organ reproduksi, hormonal dan nutrisi. Penelitian ini dilakukan terhadap strain ikan lele Mutiara (Clarias gariepinus) betina menggunakan dua bahan yang dicampur pada pakan induk, yaitu oocyte developer (Oodev) yang mengandung hormon PMSG dan antidopamin, dan karotenoid astaxanthin. Eksperimen yang dirancang adalah K (Kontrol), A50 (Astaxanthin 50 mg/kg pakan), A100 (Astaxanthin 100 mg/kg pakan), Od0.5 (Oodev 0,5 mL/kg induk untuk 2 minggu), Od1 (Oodev 1 mL/kg induk untuk 2 minggu), Od0.5A50 (kombinasi Od0.5 dan A50), Od1A50 (kombinasi Od1 dan A50), Od0.5A100 (kombinasi Od0.5 dan A100), dan Od1A100 (kombinasi Od1 dan A100). Penelitian ini dilakukan dengan memberi makan dua belas minggu. Performa reproduksi terbaik didapat pada perlakuan Od1A100. Od1A100 memiliki indeks hepatosomatik (HSI) dan gonadosomatik (HSI) tertinggi (P <0,05), juga diameter telur paling cepat besar (p <0,05), periode rematurasi terpendek, dan proporsi induk matang gonad tertinggi. Hasil ini menunjukkan bahwa Oodev dan astaxanthin dapat mempercepat kematangan gonad pada induk betina ikah lele Mutiara. Keyword: Induk, hormon, reproduksi, oocyte developer, astaxanthin 

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