Fungal Diversity in the Rhizosphere and Rhizoplane of Okra (Abelmoschus esculentus L.) Moench. in Nsukka, Enugu State, Nigeria

Aim: The growth and development of economically important crops are usually affected positively or negatively by the microbes present in the rhizosphere and rhizoplane. Based on this, the study was carried out to determine the fungal diversity in the rhizosphere and rhizoplane of okra plant. Methods: Okra seeds were purchased from an agricultural shop in Nsukka main market and were planted at Botanic garden, Department of Plant Science and Biotechnology, University of Nigeria, Nsukka. Physicochemical properties of the soil sample were evaluated prior to planting and as the plant aged. Rhizosphere and rhizoplane samples were collected at two weeks interval and dilution plate method was used in fungi isolation after which they were identified. The frequency of occurrence and the colony forming unit per gram of the sample (cfu/g) were evaluated. Results: The Physicochemical properties of the soil samples fluctuated as plant aged at two weeks intervals. The pH was slightly acidic to neutral which is ideal for most plant to grow. The water retention capacity, moisture content and organic matter content increased from 11.47-27.90 ml/g, 5.03-21.07% and 2.35-3.68% respectively at two weeks interval but fluctuates at subsequent weeks. A total of eleven (11) fungi were isolated from the rhizosphere and were identified as, Aspergillus niger, Aspergillus fumigatus, Aspergillus flavus, Aspergillus bertholletius, Fusarium oxysporum, Galactomyces candidum, Helminthosporium solani, Rhizopus stolonifer, Mucor racemosus, Penicillium citrinum and Trichoderma viride. All fungi were present in the rhizoplane except Aspergillus bertholletius and Penicillium citrinum. Rhizosphere had a greater number of fungi than the rhizoplane. Aspergillus species were predominant in both the rhizosphere and rhizoplane. A. niger had the highest frequency of occurrence of 58.67% on the 6th week and 65.79% on the 4th week in rhizosphere and rhizoplane respectively. The colony forming unit (cfu/g) of Aspergillus niger was significantly different from all other isolates at P≤0.001 followed by Mucor racemosus and Fusarium oxysporum with significant differences at P≤0.05 and P≤0.01 respectively. Conclusion: The rhizosphere and rhizoplane of okra plants has been shown to be rich in fungal diversity and a greater number were obtained from the rhizosphere. The data obtained from this work could be exploited by microbial ecologist to ascertain ecological associations and biomass increase by the fungal communities which also forms part of ecosystem. The fungi had no pathological effect on the plant which suggest positive effect on the growth and development of okra plant as the plant aged.

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Physicochemical Properties of Handere Clays and Their Use as a Building Material

Journal of Chemistry ◽

10.1155/2015/374245 ◽

2015 ◽

Vol 2015 ◽

pp. 1-6 ◽

Cited By ~ 4

Author(s):

Yasin Erdoğan

Keyword(s):

Physicochemical Properties ◽

Building Materials ◽

Raw Materials ◽

Organic Matter Content ◽

Volcanic Glass ◽

Mineralogical Composition ◽

Matter Content ◽

Loss On Ignition ◽

X Ray Diffraction ◽

Clay Deposits

Handere clay deposits were discovered at Adana in Turkey. These clay units primarily consist of uncoloured claystone, pebbly sandstone, sandstone, siltstone, and mudstone marl and include gypsum lenses and clay levels of various thicknesses in places. The physicochemical properties of these clays have been investigated by different techniques including Scanning Electron and Elemental Analysis (SEM and EDS), mineralogical analyses, chemical and physical analyses, X-ray diffraction (XRD), thermogravimetric differential thermal analysis (TG-DTA), and Atterberg (Consistency) Limits Test. The mineralogical composition deduced from XRD is wide (smectite + palygorskite + illite ± feldspar ± chlorite ± quartz ± calcite ± serpentine) due to the high smectite contents (≈85%). SEM studies reveal that smectite minerals are composed of irregular platy leaves and show honeycomb pattern in the form of wavy leaves in places. The leaves presenting an array with surface edge contact are usually concentrated in the dissolution voids and fractures of volcanic glass. Organic matter content and loss on ignition analysis of raw materials are good for all the studied samples. In summary, Handere clays can be used as building materials in bricks, roof tiles, and cement and as a binder.

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Qualidade sanitária e fisiológica de sementes de abóbora variedade Menina Brasileira

Fitopatologia Brasileira ◽

10.1590/s0100-41582006000200006 ◽

2006 ◽

Vol 31 (2) ◽

pp. 158-163 ◽

Cited By ~ 4

Author(s):

Derblai Casaroli ◽

Danton C. Garcia ◽

Marlove F. B. Muniz ◽

Nilson L. de Menezes

Keyword(s):

Aspergillus Niger ◽

Fusarium Oxysporum ◽

Alternaria Alternata ◽

Penicillium Digitatum ◽

Cucurbita Moschata ◽

Rhizopus Stolonifer ◽

Cladosporium Cucumerinum

O trabalho teve como objetivos avaliar e correlacionar a qualidade sanitária e fisiológica de sementes de abóbora, variedade Menina Brasileira (Cucurbita moschata.). Foram avaliados dois lotes de sementes de abóbora produzidas no sistema agroecológico e quatro no sistema convencional, com e sem tratamento químico. Os lotes foram submetidos aos testes de sanidade, seguindo a metodologia do "Blotter test", com congelamento, germinação e vigor (primeira contagem, índice de velocidade de germinação, envelhecimento acelerado e emergência de plântulas). Os resultados indicaram a separação dos lotes de diferentes origens a partir da qualidade sanitária e fisiológica, onde as maiores incidências de fungos foram observadas nos lotes agroecológicos e o maior potencial fisiológico foi observado nos lotes de origem convencional não tratados. Foram encontrados os fungos Fusarium oxysporum, Alternaria alternata, Cladosporium cucumerinum, Aspergillus niger, Penicillium digitatum, Rhizopus stolonifer e Phoma terrestris. A qualidade sanitária não interferiu na qualidade fisiológica das sementes de abóbora, variedade Menina Brasileira.

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Mycoflora in Commercial Pet Foods

Journal of Food Protection ◽

10.4315/0362-028x-64.5.741 ◽

2001 ◽

Vol 64 (5) ◽

pp. 741-743 ◽

Cited By ~ 13

Author(s):

DANTE J. BUENO ◽

JULIO O. SILVA ◽

GUILLERMO OLIVER

Keyword(s):

Aspergillus Niger ◽

Moisture Content ◽

Aspergillus Flavus ◽

Aspergillus Terreus ◽

Animal Health ◽

Mold Growth ◽

Mucor Racemosus ◽

Isolation Frequency ◽

Mycelia Sterilia ◽

Made In

This article reports on the identification of mycoflora of 21 dry pet foods (12 belonging to dogs and 9 to cats) that corresponded to 8 commercial brands made in Argentina and imported. The isolation frequency and relative density of the prevalent fungal genera are compared too. Ten genera and fungi classified as Mycelia sterilia were identified. The predominant genera were Aspergillus (62%), Rhizopus (48%), and Mucor (38%). The most prevalent among Aspergillus was Aspergillus flavus followed by Aspergillus niger and Aspergillus terreus. The predominant Mucor was Mucor racemosus followed by Mucor plumbeus and Mucor globosus. The moisture content of these foods ranged from 5.6 to 10.0% and from 7.2 to 9.9% for dog and cat foods, respectively. A greater moisture content in food for the senior category (9.5 ± 0.2) was observed only in comparison to adult and kitten/puppy. If the moisture content can be maintained at these levels, mold growth would be prevented or at least it would remain at an insignificant level. Some genera and species isolated and identified from the foods analyzed are potentially producing toxins, which are known as mycotoxins. This involves a risk for animal health.

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Influence of Zinc Oxide Nanoparticles on Soil Physicochemical Properties and Arachis hypogea Rhizosphere Microbial Community

International Journal of Plant & Soil Science ◽

10.9734/ijpss/2020/v32i830333 ◽

2020 ◽

pp. 88-100

Author(s):

Progress Oghenerume ◽

Samuel Eduok ◽

Basil Ita ◽

Ofonime John ◽

Inemesit Bassey

Keyword(s):

Zinc Oxide ◽

Physicochemical Properties ◽

Soil Type ◽

Organic Matter Content ◽

Matter Content ◽

Phosphorus Concentration ◽

Soil Physicochemical Properties ◽

Amended Soils ◽

Loam Soil ◽

Amended Soil

We evaluated the effect of 4000 mg zinc oxide (ZnO, 99%, 30 nm) nanoparticle on the physicochemical and microbiological properties of organic manure amended ultisol and loam soil cultivated with Arachis hypogaea using standard methods. The results indicate varying effects on the physicochemical properties in relation to the soil type. The pH of the control ultisol at 7.85 ± 0.17 and 8.3 ± 0.12 in the amended ultisol whereas, the control loam was 7.15 ± 0.17 and 7.41 ± 0.11 in the amended soil indicating 1.06- and 1.04-times higher difference than the controls respectively. Phosphorus concentration at 57.82 ± 0.54%, 50.81 ± 0.22% and 55.97 ± 0.04%, 59.97 ± 0.02% was 1.14 times lower in the ZnO amended ultisol and 1.07 times higher in amended loam soil compared to the respective controls. The organic matter content in the control and amended ultisol was 2.28 ± 0.32% and 0.91 ± 0.02%, 3.68 ± 0.36% and 0.36 ± 0.02% in the control and amended loam soil. The concentration of nitrate in the control ultisol was 0.05 ± 0.01% and 0.03 ± 0.01% in the amended soil. The nitrate in the control loam soil was 0.08 ± 0.01% relative to 0.02 ± 0.01% in the treated soil and these differences were significant at p = 0.05. The concentration of nutritive salts was reduced and in contrast iron, copper, exchangeable acids, exchange capacity, clay and silt increased in the amended soils. Further to this, heterotrophic ammonia and nitrate-oxidizing bacterial population were inhibited in the amended soils and denitrifying organisms were stimulated. The organisms were members of the genera Pseudomonas, Xanthobacter, Enterobacter, Bacillus, Lactobacillus, Citrobacter, Nitrosomonas, Agromyces and Rhizobium. ZnO nanoparticles altered the soil physicochemical properties which exacerbated the negative effect on microbial abundance and varied with the soil type.

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Biological control of brinjal wilt caused by Fusarium oxysporum f. sp. melongenae using soluble powder formulation of Aspergillus niger

10.22192/ijarbs.2017.04.11.009 ◽

2017 ◽

Vol 4 (11) ◽

pp. 66-71

Author(s):

Valmik M. Patil ◽

◽

Kishor R. Patole ◽

Mohan S. Paprikar ◽

Jaysingh C. Rajput ◽

...

Keyword(s):

Biological Control ◽

Aspergillus Niger ◽

Fusarium Oxysporum ◽

Powder Formulation

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Evaluation of the potential of feedstock combinations and their biochars for soil amendment

Waste Management & Research ◽

10.1177/0734242x211060611 ◽

2021 ◽

pp. 0734242X2110606

Author(s):

Maliheh Fouladidorhani ◽

Mohammad Shayannejad ◽

Emmanuel Arthur

Keyword(s):

Physicochemical Properties ◽

Soil Amendment ◽

Particle Density ◽

Soil Amendments ◽

Organic Matter Content ◽

Total Porosity ◽

Matter Content ◽

Cow Manure ◽

N Content ◽

Pine Wood

One of the approaches for recycling and reusing agricultural and animal wastes is to pyrolyse the residues and subsequently use them as soil amendments. The prevalence of several feedstocks suggests that it is necessary to investigate the optimal combinations of feedstocks and pyrolysis temperature for use as soil amendments. This study was done to evaluate five combinations of raw materials (sugarcane bagasse, rice husk, cow manure and pine wood) and their biochars produced by slow pyrolysis at 300°C and 500°C for soil amendment. Several physicochemical properties (electrical conductivity (EC), pH, cation exchange capacity (CEC), total organic matter content (C) total porosity (TP), total nitrogen (N), particle density (PD) and bulk density (BD)) were investigated. Comparison among feedstocks showed that the highest PD, BD and CEC were observed in WM (cow manure-pine wood). The pyrolysis process increased the PD, TP, N and monovalent cations and decreased EC, CEC and BD. Compared to the feedstock, pyrolysis increased the N content, but higher temperatures lowered the N content. Pyrolysis at 500°C reduced the EC, N, CEC and biochar yield by 18%, 13%, 21% and 24% respectively, compared to 300°C. Pyrolysis at 500°C increased the pH, Na+ and K+ by 17%, 12% and 22%, respectively, compared to 300°C. Considering the physicochemical properties of biochar and the costs, the bagasse-wood-rice (BWR) combination and temperature of 300°C are suggested for biochar production for soil amendment.

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The role of gum induced by Fusarium oxysporum Schlecht. Snyd. et Hans. f.sp. tulipae Apt. in tulip bulbs on growth and development of the pathogen

Plant Protection Science ◽

10.17221/10515-pps ◽

2017 ◽

Vol 38 (SI 2 - 6th Conf EFPP 2002) ◽

pp. 432-435

Author(s):

A. Saniewska

Keyword(s):

Fusarium Oxysporum ◽

Growth And Development ◽

Mycelium Growth ◽

Tulip Bulbs ◽

Bacto Agar

It was showed that gums induced by Fusarium oxysporum f.sp. tulipae in tulip bulbs applied to mineral Czapek-Dox Broth with Bacto Agar (CzDA) medium, containing sucrose substantially stimulated abundant growth of mycelium and sporulation of the pathogen. Addition of arabinose, xylose and their mixture, sugars occurring in tulip gum polysaccharide, to CzDA medium caused that mycelium was very sparse and sporulation was only slightly stimulated by arabinose. Mycelium growth on mineral CzDA medium without sucrose, was poor and sparse but addition of gum to the medium caused formation of abundant mycelium and increased sporulation of the pathogen. It is possible that polysaccharide of tulip gum may act mainly as elicitor and partially as substrate in regulation of mycelium growth and sporulation of Fusarium oxysporum f.sp. tulipae.

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Effect of Biogas Digestate and Mineral Fertilisation on the Soil Properties and Yield and Nutritional Value of Switchgrass Forage

Agronomy ◽

10.3390/agronomy10040490 ◽

2020 ◽

Vol 10 (4) ◽

pp. 490

Author(s):

Aleksandra Głowacka ◽

Bogdan Szostak ◽

Renata Klebaniuk

Keyword(s):

Physicochemical Properties ◽

Panicum Virgatum ◽

Nutritional Value ◽

Organic Matter Content ◽

Application Rate ◽

Matter Content ◽

Forage Yield ◽

Biogas Digestate ◽

Green Fodder ◽

Silty Loam

The aim of this study was to assess the effect of a three-year application of digestate from an agricultural biogas plant on the physicochemical properties of highly acidic pHKCl 4.4 ± 0.23, silty loam soils with low macronutrient content and on the yield and nutritional value of switchgrass (Panicum virgatum L.) biomass harvested for green fodder. The experiment included the following treatments: (1) O (control)—no fertilisation, (2) NPK—mineral fertilisation with (in kg ha−1) 150 N, 53.0 P and 105 K, (3) biogas digestate at 30 m3 ha−1 and (4) biogas digestate at 60 m3 ha−1. The higher application rate of biogas digestate significantly reduced soil acidity to pHKCl 4.9 ± 0.18 and improved its sorption properties. It also increased the soil organic matter content from 5.6 ± 0.21 to 6.4 ± 0.22 g Corg kg−1 and of K and Zn. The higher level of biogas digestate significantly increased switchgrass yield to 5.15 ± 0.26 t ha−1. The lower application rate of biogas digestate resulted in forage yield of 4.30 ± 0.20 t ha−1 comparable to that obtained after mineral fertilisation (4.33 ± 0.22 t ha−1). Following application of mineral fertilisers and the higher level of biogas digestate, the number of panicles per plant (150 ± 2.49–157 ± 0.6.17), panicle height (107 ± 1.98–114 ± 2.08), crude ash content (61.2 ± 0.43–65.5 ± 0.38) and protein content (106 ± 0.59–92 ± 1.11) in the switchgrass biomass from the first cut were higher than in the case of unfertilised soil (110 ± 3.81, 93 ± 1.32, 55.5 ± 0.40, 80.3 ± 0.37). The use of mineral fertilisers and biogas digestate increased the content of protein, P and Mg in biomass from the second cut. The results suggest that the use of digestate improved the physicochemical properties of highly acidic soil and increased the yield of switchgrass forage without diminishing its nutritional value.

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Effects of Garlic Extract, Allamanda Leaf Extract and Provax -200 on Seed Borne Fungi of Rice

The Agriculturists ◽

10.3329/agric.v10i1.11064 ◽

2012 ◽

Vol 10 (1) ◽

pp. 46-50 ◽

Cited By ~ 3

Author(s):

F. Yeasmin ◽

M. Ashrafuzzaman ◽

I. Hossain

Keyword(s):

Aspergillus Niger ◽

Seed Germination ◽

Fusarium Oxysporum ◽

Aspergillus Flavus ◽

Fusarium Moniliforme ◽

Leaf Extract ◽

Allium Sativum ◽

Bipolaris Oryzae ◽

Rice Varieties ◽

Nigrospora Oryzae

Seeds of rice were treated with Garlic (Allium sativum) clove extract @ 1:0, 1:1, 1:2 dilutions in water, allamanda (Allamanda cathartica) leaf extract @ 1:1, 1:2, 1:3 dilutions in water and Provax-200 @ 0.3% for controlling seed borne fungi, where the seed samples of three rice varieties viz. Katharee, Gutee Aus and Kalijira were collected from farmers storages of Bangladesh. The seed germination under control ranged from 64 to 77%, where treatments resulted up to 100% germination. The identified seed borne fungi of rice were Bipolaris oryzae, Curvularia oryzae, Fusarium oxysporum, F. moniliforme, Nigrospora oryzae, Aspergillus flavus, Aspergillus niger and Penicillium sp., where prevalence of Bipolaris oryzae (7.5%) and Fusarium moniliforme (8.3%) were the maximum. All the treatments significantly reduced the seed borne fungi up to 100% over the control, where Provax was found best and was statically similar to garlic (1:1) extract against seed borne pathogen of rice.DOI: http://dx.doi.org/10.3329/agric.v10i1.11064The Agriculturists 2012; 10(1): 46-50

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Antimicrobial activity of Cressa cretica L., a Halophytic Plant

Journal of Scientific Research ◽

10.3329/jsr.v4i1.7719 ◽

2011 ◽

Vol 4 (1) ◽

pp. 203 ◽

Cited By ~ 1

Author(s):

P. Sunita ◽

S. Jha ◽

S. P. Pattanayak ◽

S. K. Mishra

Keyword(s):

Candida Albicans ◽

Aspergillus Niger ◽

Fusarium Oxysporum ◽

Candida Tropicalis ◽

Diffusion Method ◽

Zone Of Inhibition ◽

Biochemical Alterations ◽

Agar Disc ◽

Aspergillus Fumigates ◽

Halophytic Plant

In the present investigation different fractions (hexane, ethylacetate and methanol) of the whole methanolic extract of Cressa cretica L.(Convolulaceae), a widely grown halophytic plant were studied against wide ranges of bacteria (both positive and negative strain) and five fungi Candida albicans, Candida tropicalis – dimorphic fungi, Aspergillus fumigatus, Aspergillus niger – systemic fungi and fusarium oxysporum by agar disc diffusion method. Among the three fractions the ethylacetate fraction of C.cretica showed the highest activity, but among the pathogens highest activity was revealed against Escherichia coli, Klebsiella pneumoniae (zone of inhibition diameter in mm was found to be 26 and 31, respectively). The ethylacetate fraction was sensitive to both gram +ve and gram –ve bacterias. C.cretica showed higher inhibitory activity against the Aspergillus fumigates, Aspergillus niger (zone of inhibition diameter in mm was found to be 26 and 22, respectively) than the Candida albicans and Candida tropicalis and least was found for Fusarium oxysporum. As ethylacetate fraction revealed better activity than other fractions, further study of morphological and biochemical alterations were carried out only with ethylacetate fraction and the results revealed some morphological and biochemical alterations, which indicates that ethylacetate fraction of C. cretica possess potential broad spectrum antimicrobial activity.Keywords: Cressa cretica; Antifungal; Gram positive bacteria; Gram negative bacteria; Biochemical alterations.© 2012 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved.doi: http://dx.doi.org/10.3329/jsr.v4i1.7719J. Sci. Res. 4 (1), 203-212 (2012)

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