scholarly journals Prevalence of Geohelminth Parasites of Horses in Dutsinma Metropolis, Katsina State Nigeria

2019 ◽  
pp. 1-7
Author(s):  
J. B. Orpin ◽  
I. Mzungu ◽  
M. S. Hussaini
Keyword(s):  
Health Education ◽  
Fecal Sample ◽  
Total Sample ◽  
Sample Collection ◽  
Managerial Control ◽  
Fresh Fecal Sample ◽  
Young Horses

This study was carried out to investigate the prevalence of geohelminth parasites of horses in Dutsinma metropolis between May to August, 2016. Fresh fecal sample were obtained from the rectum of 48 randomly selected horses. A floatation technique was used for the lab work experiment. From the result, out of the total sample collected 6 (12.50%) were positive with eggs of two parasites Strongyles and Oxyuris spp with the prevalence of (6.25%) for each. Among the three points of sample collection, the highest prevalence was recorded at Kadangaru (20.00%) followed by Unguwa Yandaka (18.18%) and no case recorded at Hayingada. X2 cal = 36.84 at P=0.05 and 2df. The highest prevalence was recorded among the males (18.52%) while only 1 female was affected with the prevalence of (4.76%) X2 cal = 36.57 at P=0.05 and 2df. Prevalence was recorded in adult of 14.29% while young horses recorded a prevalence of 10.00%, X2 cal = 36.84 at P=0.05 Strongyles and Oxyuris spp were recognized as important geohelminth parasites in Horses in Dutsinma Local Goverment Area of Katsina State with various rates of infection (12.50%). Managerial control tools should be integrated to improve prevention of geohelminth parasites by communication, information and health education.

scholarly journals Investigating the Strongyle Populations, with Emphasis on Strongylus vulgaris (Nematoda: Strongylidae) in Romanian Horses, Based on Larval Cultures

2017 ◽  
Vol 74 (2) ◽  
pp. 186
Author(s):  
Marius Catalin BUZATU ◽  
Ioan Liviu MITREA ◽  
Alexandra GRUIANU ◽  
Mariana IONITA
Keyword(s):  
Fecal Sample ◽  
Morphological Identification ◽  
Sensitive Technique ◽  
Strongylus Vulgaris ◽  
North Eastern ◽  
Third Stage ◽  
Horse Farms ◽  
Fresh Fecal Sample ◽  
Mcmaster Method

This study aimed to investigate the presence of Strongylus vulgaris on different horse establishments in Romania. A total number of 380 horses were enrolled in the study, representing: three stud farms (n=156); four sport/recreational establishments (n=75); and working horses (n=149). Overall, 106 premises/yards from 17 localities in 9 counties from North-Eastern, Center and Southern Romania were visited. Fresh fecal sample were collected from individual horses and were analyzed by a modified McMaster method for fecal strongyle egg per gram counting. Further, larval cultures were performed for morphological identification of S. vulgaris third stage larvae. Overall, of the investigated horses from studs, sport/recreational units, and working horses, 84.60%, 44.0%, and 79.20%, respectively were positive for strongyle. On coprocultures, cyathostomin-larvae were the most prevalent in all horse-establishment types, while S. vulgaris larvae were found only in working horses (12.1%; n=18). Other strongylin species have been identified, namely S. equinus, S. edentatus, Oesophagodontus robustus, and Triodontophorus spp. all in working horses, and Craterostomum acuticaudatum, in both working horses and horses for sport units. However, further studies are planned by using more sensitive technique for monitoring of S. vulgaris in Romanian horse farms.

scholarly journals PENGARUH PENDIDIKAN KESEHATAN TERHADAP PENGETAHUAN DAN KETERAMPILAN DALAM PERAWATAN PAYUDARA PADA IBU POST PARTUM DI RS Dr. OEN SURAKARTA

2015 ◽  
Vol 1 (2) ◽  
Author(s):  
Sri Aminingsih ◽  
Warsini Warsini ◽  
Umi Padmiati
Keyword(s):  
Data Analysis ◽  
Health Education ◽  
Post Partum ◽  
Total Sample ◽  
Study Data ◽  
Life Goals ◽  
Process Of Change ◽  
Breast Care

Background. Health education is a planned process of change in behavior onthe individual or community to be more self-sufficient in achieving life goals se,attitudes and community practices in maintaining and improving health.The purpose to determine the effect of health education on the knowledge andskills in the treatment of breast in women post partumMethod using pre post experimental with a total sample of 25 respondents. Theresult showed no conclusions on the effect of health education knowledge andskills in the treatment of breast in women postpartum hospital. Dr. OenSurakarta. This is evidenced by the amount of 0.01 t α = 9.313 and t table =2.797 for knowledge, while for the results obtained skills t α 0,01 = 10.303 and ttable = 2.797. This study data analysis using Paired Test.The conclusion. There pendidikanpendidikan influence the health of theknowledge and skills in the treatment of breast in women postpartum in RS Dr.Oen Surakarta.Keywords : Health education, knowledge of breast care and breast care skills

Urine and fecal sample collection on filter paper for ovarian hormone evaluations

1995 ◽  
Vol 37 (4) ◽  
pp. 305-315 ◽  
Author(s):  
S. E. Shideler ◽  
C. J. Munro ◽  
H. K. Johl ◽  
H. W. Taylor ◽  
B. L. Lasley
Keyword(s):  
Filter Paper ◽  
Fecal Sample ◽  
Ovarian Hormone ◽  
Sample Collection

scholarly journals Comparison of Methods To Collect Fecal Samples for Microbiome Studies Using Whole-Genome Shotgun Metagenomic Sequencing

mSphere ◽  
2020 ◽  
Vol 5 (1) ◽  
Author(s):  
Doratha A. Byrd ◽  
Rashmi Sinha ◽  
Kristi L. Hoffman ◽  
Jun Chen ◽  
Xing Hua ◽  
...  
Keyword(s):  
Ambient Temperature ◽  
Fecal Sample ◽  
Gold Standard ◽  
Beta Diversity ◽  
Whole Genome ◽  
Metagenomic Sequencing ◽  
Sample Collection ◽  
Fecal Samples ◽  
Shotgun Metagenomic Sequencing ◽  
Collection Methods

ABSTRACT Few previous studies have assessed stability and “gold-standard” concordance of fecal sample collection methods for whole-genome shotgun metagenomic sequencing (WGSS), an increasingly popular method for studying the gut microbiome. We used WGSS data to investigate ambient temperature stability and putative gold-standard concordance of microbial profiles in fecal samples collected and stored using fecal occult blood test (FOBT) cards, fecal immunochemical test (FIT) tubes, 95% ethanol, or RNAlater. Among 15 Mayo Clinic employees, for each collection method, we calculated intraclass correlation coefficients (ICCs) to estimate stability of fecal microbial profiles after storage for 4 days at ambient temperature and concordance with immediately frozen, no-solution samples (i.e., the putative gold standard). ICCs were estimated for multiple metrics, including relative abundances of select phyla, species, KEGG k-genes (representing any coding sequence that had >70% identity and >70% query coverage with respect to a known KEGG ortholog), KEGG modules, and KEGG pathways; species and k-gene alpha diversity; and Bray-Curtis and Jaccard species beta diversity. ICCs for microbial profile stability were excellent (≥90%) for fecal samples collected via most of the collection methods, except those preserved in 95% ethanol. Concordance with the immediately frozen, no-solution samples varied for all collection methods, but the number of observed species and the beta diversity metrics tended to have higher concordance than other metrics. Our findings, taken together with previous studies and feasibility considerations, indicated that FOBT cards, FIT tubes, and RNAlater are acceptable choices for fecal sample collection methods in future WGSS studies. IMPORTANCE A major direction for future microbiome research is implementation of fecal sample collections in large-scale, prospective epidemiologic studies. Studying microbiome-disease associations likely requires microbial data to be pooled from multiple studies. Our findings suggest collection methods that are most optimal to be used standardly across future WGSS microbiome studies.

Colonization Levels of Streptococcus Mutans between Mother and Infant: A Postnatal Prospective Cohort Study

2014 ◽  
Vol 38 (3) ◽  
pp. 197-200 ◽  
Author(s):  
S Ruiz-Rodriguez ◽  
V Lacavex-Aguilar ◽  
M Pierdant-Perez ◽  
P Mandeville ◽  
M Santos-Diaz ◽  
...  
Keyword(s):  
Cohort Study ◽  
Prospective Cohort Study ◽  
Prospective Cohort ◽  
Direct Relationship ◽  
Total Sample ◽  
Test Results ◽  
Sample Collection ◽  
Gram Staining ◽  
Activity Test ◽  
Oral Sample

Objective: To investigate the possible association between maternal S. mutans levels and those of the infant during the period between birth and 5 months and evaluate possible risk factors in the S mutans colonization. Study Design: A prospective cohort study was carried out comprising 62 infants and their mothers, selected at the time of childbirth. For each infant, a sample swab was taken at 0, 15, 30, 90, and 150 days postpartum; on the same days, a sample was obtained from the mothers. TYCSB medium was employed for identifying the microorganism, which was later confirmed by Gram staining, the catalase activity test, and the API strep test. Results: The final total sample consisted of 60 infants, from which S. mutans was detected in only 2 (3%) at the 150th day of oral sample collection. Of the sample of 60 mothers, 54 exhibited colonization levels. Conclusions: In the studied sample pairs up to 150 days, it was not possible to demonstrate the presence of a direct relationship between maternal S. mutans oral levels.

scholarly journals Frequency of Gingivitis among 12 to 70 Years Old Patients Visiting Lady Reading Hospital, Peshawar, Pakistan: A Cross Sectional Study

2021 ◽  
Vol 2 (11) ◽  
pp. 1074-1077
Author(s):  
Gulrukh Hamid ◽  
Asim Shahzad ◽  
Abrar Hussain Mian
Keyword(s):  
Sampling Technique ◽  
Total Sample ◽  
Cross Sectional Study ◽  
Sectional Study ◽  
Sample Collection ◽  
Cross Sectional ◽  
Old Patients ◽  
Hygiene Practices ◽  
Health Complications ◽  
Health Organization

The present cross sectional study aimed to determine the frequency of gingivitis among 21 to 70 old patients at Periodontal Department of Lady Reading Hospital (LRH), Peshawar, Pakistan. A total of 133 patients were observed by using Word Health Organization (WHO) sample size calculator with the following assumption, confidence level = 95%, Anticipated absolute precision = 8% and Population proportion = 33.4%. Standard proforma (questionnaire) and non-probability consecutives sampling technique was used for the sample collection with inclusion and exclusion base criteria for the study fulfillment. Results showed that mean age was 60 years with standard deviation + 8.34. 63 (47.36%) of patients were male and 70 (52.63%) were female. Male patients had gingival inflammation affected more 50 (79.36%) as compared to female 17 (24.28%). Total sample prevalence of mild, moderate and sever gingivitis was 43 (64.17%), 20 (29.85%) and 4 (5.97%). Percentage of mild gingivitis in females was less than males (58.82% vs. 66%), moderate gingivitis percentage was also slightly less in females than males (29.41% vs. 30%). However percentage of females having severe gingivitis was found higher than males (11.76% vs. 4%). Out of 133 patients 67 (50.37%) were analyzed as gingivitis while 66 (49.62%) patients were without gingivitis. It was revealed that the incidence rate of gingivitis was estimated 67 (50.37%) among 133 patients. Patients should need awareness of gingivitis through proper education channel as a result to adopt appropriate oral hygiene practices and other preventive measures to reduce the incidence of this disease and other oral health complications in future.

scholarly journals Fecal Sample Collection Methods and Time of Day Impact Microbiome Composition and Short Chain Fatty Acid Concentrations

2021 ◽  
Author(s):  
Jacquelyn Jones ◽  
Stacey Reinke ◽  
Alishum Ali ◽  
Debra Palmer ◽  
Claus T. Christophersen
Keyword(s):  
Fatty Acid ◽  
Fecal Sample ◽  
Short Chain Fatty Acid ◽  
Time Of Day ◽  
Chain Fatty Acid ◽  
Short Chain ◽  
Its2 Region ◽  
Rrna Gene ◽  
Sample Collection ◽  
Collection Methods

Abstract Associations between the human gut microbiome and health outcomes continues to be of great interest, although fecal sample collection methods which impact microbiome studies are sometimes neglected. Here, we expand on previous work in sample optimization, to promote high quality microbiome data. To compare fecal sample collection methods, amplicons from the bacterial 16S rRNA gene (V4) and fungal (ITS2) region, as well as short chain fatty acid (SCFA) concentrations were determined in fecal material over three timepoints. We demonstrated that spot sampling of stool results in variable detection of some microbial members, and inconsistent levels of SCFA; therefore, sample homogenization prior to subsequent analysis or subsampling is recommended. We also identify a trend in microbial and metabolite composition that shifts over two consecutive stool collections less than 25h apart. Lastly, we show significant differences in bacterial composition that result from collecting stool samples in OMNIgeneGUT tube (DNA Genotec) or Stool Nucleic Acid Collection and Preservation Tube (NORGEN) compared to immediate freezing. To assist microbiome investigators plan their fecal sample collection and storage procedures for multiple analyses, we recommend participants to collect the first full bowel movement of the day and freeze the sample immediately after collection.

scholarly journals Parents’ Effort to Prevent Toddler Injury at Home

2017 ◽  
Vol 4 (1) ◽  
pp. 012-016
Author(s):  
Agus Khoirul Anam ◽  
Arif Mulyadi ◽  
Devi Widyawati Sagar
Keyword(s):  
Health Education ◽  
Injury Prevention ◽  
Cause Of Death ◽  
Sampling Technique ◽  
Total Sample ◽  
Quota Sampling ◽  
Provide Health Education ◽  
Children Under 5 ◽  
At Home

The injury is the leading cause of death in children. Many children are hospitalized, becoming disable or death because of the injury. The purpose of research was to describe the Par- ents’ effort to prevent toddler injury at Home. Methods in research used descriptive design. The population in this study was all of parents with children under 5 years in the Posyandu Asparaga Bendogerit Sananwetan. The total sample was 43 parents by using quota sampling technique. The data was collected by a questionnaire designed by the researchers. The results of this study showed parents effort generally was in fair category 55,8% of parents. In this study, the injury prevention was still lack such as cleaning the floor so that became not slippery, avoiding the use of tablecloths, not leaving the child alone in the bathroom and no furniture with angled taper. Health employee need to provide health education about the effort of parents in the prevention of the injury of tod- dler at home.

scholarly journals Evaluating live microbiota biobanking using an ex vivo microbiome assay and metaproteomics

2021 ◽  
Author(s):  
Xu Zhang ◽  
Krystal Walker ◽  
Janice Mayne ◽  
Leyuan Li ◽  
Zhibin Ning ◽  
...  
Keyword(s):  
Fecal Sample ◽  
Functional Activity ◽  
Fecal Microbiota Transplantation ◽  
Ex Vivo ◽  
Fecal Microbiota ◽  
Rapid Evaluation ◽  
Sample Collection ◽  
Functional Responses ◽  
Clinical Investigations

Biobanking of live microbiota is becoming indispensable for mechanistic and clinical investigations of drug-microbiome interactions and fecal microbiota transplantation. However, there is a lack of methods to rapidly and systematically evaluate whether the biobanked microbiota maintains their cultivability and functional activity. In this study, we use a rapid ex vivo microbiome assay and metaproteomics to evaluate the cultivability and the functional responses of biobanked microbiota to treatment with a prebiotic (fructo-oligosaccharide, FOS). Our results indicate that the microbiota cultivability and their functional responses to FOS treatment were well maintained by freezing in a deoxygenated glycerol buffer at -80°C for 12 months. We also demonstrate that the fecal microbiota is functionally stable for 48 hours on ice in a deoxygenated glycerol buffer, allowing off-site fecal sample collection and shipping to laboratory for live microbiota biobanking. This study provides a method for rapid evaluation of the cultivability of biobanked live microbiota. Our results show minimal detrimental influences of long-term freezing in deoxygenated glycerol buffer on the cultivability of fecal microbiota.

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