Effect of Simvastatin, Hydrochlorothiazide and Combined Treatment on Bone Formation and Lipid Profile of Corticosteroid-Induced Osteoporosis in Albino Rats

Abstract Objective To evaluate the influence of irisin on the experimental paradigm of non-alcoholic fatty liver (NAFL) as a part of MetS cluster. Methods Forty male albino rats were divided into four groups; normal control, standard diet + irisin, high carbohydrate and fat diet (HCHF), and HCHF + irisin. After the experimental period, levels of fasting blood sugar (FBS), insulin, lipid profile, kidney functions, salusin-alpha (Sal-α), adropin, and retinol-binding protein-4 (RBP-4) were evaluated. Peroxisome proliferator-activated receptor-gamma coactivator-1alpha (PGC-1α) expression in skeletal muscle was evaluated by quantitative real-time PCR. Aorta, liver, pancreas, and skeletal muscle tissue samples were prepared for histopathological examination. Results Rats administrated HCHF showed elevated levels of FBS, lipid profile, kidney functions, RBP-4, and downregulation of PGC-1α expression along with a decline in levels of insulin, Sal-α, and adropin while administration of irisin significantly attenuated these levels. Conclusions Irisin as based therapy could emerge as a new line of treatment against MetS and its related diseases.

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Amelioration of histological changes and associated metabolic abnormalities by a combination of Morinda lucida and metformin in diabetic rats

The Journal of Phytopharmacology ◽

10.31254/phyto.2018.7304 ◽

2028 ◽

Vol 7 (3) ◽

pp. 253-256

Author(s):

Atanu FO ◽

◽

Momoh S ◽

Ugwu CE ◽

Ameh O ◽

...

Keyword(s):

Body Weight ◽

Lipid Profile ◽

Diabetic Complications ◽

Combined Treatment ◽

Diabetic Rats ◽

Atherogenic Index ◽

Positive Outcomes ◽

Metabolic Abnormalities ◽

Histological Changes ◽

Histological Studies

This work investigates the ability of Morinda lucida and co-administration of Morinda lucida/metformin in the control of biochemical and histological changes in alloxan-induced diabetic rats. Alloxan diabetic rats were treated with 200 mg/Kg body weight of Morinda lucida leaves extract, 1 mg/Kg BW of metformin or a combination of the two treatments for 28 days. Results of the studies revealed that Morinda lucida leaves extract significantly improved lipid profile and kidney function in diabetic rats. These positive outcomes were enhanced by combined treated with Morinda lucida leaves extract and metformin. Furthermore, the calculated atherogenic index of treated animals were close to those of normal rats as opposed to diabetic rats. Similarly, histological studies showed that Morinda lucida leaves extract and metformin administered together or singly, ameliorated damages in pancreas and kidneys from alloxan diabetic rats. It can therefore be inferred that combined treatment with Morinda lucida leaves extract and merformin could improve the potency of Morinda lucida leaves used in the management of diabetic complications

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Effects of Quercetin and Coenzyme Q10 on Biochemical, Molecular, and Morphological Parameters of Skeletal Muscle in Trained Diabetic Rats

Current Molecular Pharmacology ◽

10.2174/1874467214666210521170339 ◽

2021 ◽

Vol 14 ◽

Author(s):

Amal M. Youssef ◽

Dalia A. Mohamed ◽

Samia Hussein ◽

Doaa M. Abdullah ◽

Shaimaa A. Abdelrahman

Keyword(s):

Skeletal Muscle ◽

Coenzyme Q10 ◽

Combined Treatment ◽

Diabetic Rats ◽

Molecular Structures ◽

Treadmill Training ◽

Albino Rats ◽

Musculoskeletal Function ◽

Sugar Levels ◽

Fibronectin Type Iii Domain

Background: Diabetes mellitus (DM) affects the musculoskeletal system through its metabolic perturbations. Exercise modulates blood sugar levels and increases the body’s sensitivity to insulin in patients with DM. Objective: This study aimed to investigate the potential effects of combined quercetin and coenzyme Q10 (CoQ10) supplements with or without exercise on the histological, biochemical and molecular structures of diabetic rat’s skeletal muscle. Method: A total of 64 adult male albino rats were divided into six groups: control, trained nondiabetic, non-trained diabetic, diabetic rats treated with combined CoQ10 and quercetin, diabetic rats with treadmill training, and diabetic rats treated with treadmill training and CoQ10 and quercetin. Blood and skeletal muscle samples were obtained from all groups for routine histological examination and biochemical determination of cytokine levels and protein activities. Quantitative real-time polymerase chain reaction (qRT-PCR) and morphometric analysis of PAS and Bax expressions were also performed. Results: Biochemical analysis revealed improvement in all studied parameters with combined CoQ10 and quercetin than exercise training alone. Combined treatment and exercise showed significant improvement in all parameters especially interleukin 6 and malondialdehyde. Fibronectin type III domain-containing protein 5 (FNDC5) expression and irisin levels increased in all trained groups but combined treatment with exercise significantly increased their levels than exercise alone. Histological analysis revealed improvement after exercise or combined treatment; however, when exercise was combined with CoQ10 and quercetin, marked improvement was observed. Conclusion: the combination of CoQ10 and quercetin could be promising in preserving musculoskeletal function in patients with DM concomitantly with physical exercise.

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