scholarly journals Antibacterial Activity of Some Nano Particles on Antibiotic-resistant Bacterial Pathogens from the Air of Operation Theatre

2019 ◽  
pp. 1-8
Author(s):  
Nazish Mazhar Ali ◽  
Saiqa Andleeb ◽  
Abdullah Mazhar ◽  
Bushra Mazhar ◽  
Sehar Zahid
Keyword(s):  
Antibacterial Activity ◽  
Ferric Oxide ◽  
Bacterial Pathogens ◽  
Research Work ◽  
Bacterial Pathogen ◽  
Growth Conditions ◽  
Nano Particles ◽  
Bacterial Isolates ◽  
Bacterial Strains ◽  
Operation Theatre

The current research work was carried out to find the antibacterial activity of some nano particles against bacterial pathogens isolated from the air of operation theatre of Mayo hospital, Lahore, Pakistan. Three pathogenic bacterial strains were isolated, namely A1, A2, A3. Molecular characterisation, optimum growth conditions and antibiotic resistance of bacterial isolates were checked. The antibiotics used in this study were Amoxycillin, Cefepime and Ampicillin. Nano particles were used in methanolic solutions (mg/ml). Nano particles included ferric oxide, Zinc oxide and Silver Oxide. Results showed A3 was resistant to all antibiotics. Other strains showed sensitivity and resistance to these three antibiotics. All nano particles showed antibacterial activity against pathogenic bacterial isolates. Maximum zone of inhibition of 1 cm was formed when used Ferric oxide against the A1 bacterial pathogen. Optimum temperature was 37°C while the optimum pH was 7.  These bacterial pathogens were identified by ribotyping as Staphylococcus aureus (A1), Pseudomonas aeruginosa (A2) and Streptococcus pyogenes (A3).

scholarly journals Detection of Potential Bacterial Pathogens and Aflatoxigenic Fungi from Grain Samples

2019 ◽  
Vol 7 (5) ◽  
pp. 731
Author(s):  
Hinda Abdukadir Mohamed ◽  
Md. Salauddin ◽  
Md. Khaled Hossain ◽  
Farzana Afroz
Keyword(s):  
Preventive Measures ◽  
Research Work ◽  
Bacterial Pathogen ◽  
Bacterial Isolates ◽  
Salmonella Spp ◽  
Aflatoxigenic Fungi ◽  
Identification Techniques ◽  
Staphylococcus Spp ◽  
Effective Drugs ◽  
Klebsiella Spp

Current research work was carried out for the detection of potential bacterial pathogen and aflatoxigenic fungi Aspergillus spp. from grain comprising [Rice (5), Maize (5), Wheat (5), Khessari dal (5) and Anchora dal (5)] were collected from 3 different local markets of Dinajpur District, Bangladesh. 15 bacterial isolates comprising 4 genera of bacteria were found from a total of 25 samples. The isolated bacteria were Staphylococcus spp., Escherichia coli, Klebsiella spp., Salmonella spp. with 16%, 28%, 16% and 16% prevalence respectively. Antibiogram studies revealed that overall effective drugs against isolated bacteria were Ciprofloxacin followed by Gentamycin. But resistant drugs were Penicillin, Vancomycin, Erythromycin, Kanamycin, and Amoxicillin. The variation in the sensitivity of common antibiotic could be the result of extensive and indiscriminate use of these antibiotics. Aspergillus spp. was isolated from 4-grain samples with 16% prevalence. But aflatoxigenic Aspergillus spp. was isolated from 3 samples with 12% prevalence. From the wheat samples and maize, the aflatoxigenic fungus was isolated and their prevalence in maize, wheat was 40% and 20% respectively. Their early detection can help to take preventive measures to combat economic and health losses. The study showed that earlier detections can be made by simple traditional identifications using macro and micromorphological fungal features rather than adopting the time and cost consuming molecular identification techniques.

In-vitro evaluation of cisplatin nanoparticles encompass natural polymer

2020 ◽  
Vol 3 (1) ◽  
pp. 1-6
Author(s):  
Bhavani J ◽  
Sunil Kumar Prajapati ◽  
Ravichandran S
Keyword(s):  
Antibacterial Activity ◽  
Bacterial Infections ◽  
Pathogenic Bacteria ◽  
Bacterial Species ◽  
Research Work ◽  
Dilution Method ◽  
Bacterial Strains ◽  
Active Components ◽  
Synthetic Drugs

Opportunistic bacterial infections are common in the various parts of human body. In recent years bacterial species have shown resistance against a number of synthetic drugs. This study measured the antibacterial activity of bacterial strains against five common pathogenic bacteria related strains. Cup plate method and two fold serial dilution method were used to evaluated by antibacterial activity by the help of different bacterial related strains. The results revealed that Cisplatin (CIP) using natural as a polymer showed a minimum inhibitory concentration (MIC) at 250 mg/ml to 500 mg/ml of the broth against all bacterial strains. CIP using natural as a polymer was prepared different doses1000 μg/ml and 2000 μg/ ml and measured zone of inhibition dose dementedly reduced when compared to standard. The CIP using natural as a polymer exhibited strong anti-bacterial activity against five different species of bacteria and this may be attributed to various active components. Our research work has been indicated Nanoparticles containing CIP using natural as a polymer formulated for the enhanced anti-cancer activity through antimicrobial mechanism. 

scholarly journals BIOPROSPECTING FOR BACTERIAL ENDOPHYTES ASSOCIATED WITH ZINGIBERACEAE FAMILY RHIZOMES IN SIBOLANGIT FOREST, NORTH SUMATERA

2020 ◽  
Vol 1 (1) ◽  
pp. 27-36
Author(s):  
Jendri Mamangkey
Keyword(s):  
Antibacterial Activity ◽  
Protease Activity ◽  
Antibacterial Agent ◽  
Phosphate Solubilization ◽  
Antibacterial Activities ◽  
Chemical Diversity ◽  
Bacterial Endophytes ◽  
Bacterial Isolates ◽  
Bacterial Strains ◽  
Paper Disc

The present study was conducted aiming to isolate and characterize endophytic bacterial isolates with antibacterial ability, phosphate solubilization, and proteolytic activity from rhizomes of the Zingiberaceae family (Etlingera sp., Globba patens, Globba pendula, and Zingiber multibracteata). Nineteen bacterial isolates were obtained from Zingiberaceae rhizomes with isolate codes of EZS27, EZS18, EZS19, EZS25, EZS16, EZS08, EZS09, EZS13, EZS20, EZS14, EZS10, EZS11, EZS03, EZS05, EZS06, EZS43, EZS45, EZS47, and EZS28. The screening of the endophytes for antibacterial activity was done through the paper disc method. Four bacterial isolates presented antibacterial activities. EZS06 isolate inhibited the growth of EPEC (11 mm), P. vulgaris ATCC 13315 (10 mm), and L. monocytogenes BTCC B693 (9 mm). Also, EZS20 isolate inhibited the growth of S. aureus ATCC 29213 (17 mm), EZS28 isolate inhibited MRSA ATCC 43300 (8.6 mm), and EZS45 isolate inhibited S.  Epidermidis ATCC 12228 (9 mm). The EZS19, EZS03, and EZS16 isolates dissolved the phosphate most effectively. Eight isolates (EZS19, EZS47, EZS27, EZS25, EZS09, EZS20, EZS45, and EZS06) showed the best protease activity. In general, our results showed that the endophytic bacterial strains can be used as a new and useful antibacterial agent since it showed antibacterial activity and chemical diversity. Furthermore, it also has the potential for exploitation in a wide variety of medical, agricultural, and industrial areas.

scholarly journals Significant production of vanillin and in vitro amplification of ech gene in local bacterial isolates

2023 ◽  
Vol 83 ◽  
Author(s):  
B. Mazhar ◽  
N. Jahan ◽  
M. Chaudhry ◽  
I. Liaqat ◽  
M. Dar ◽  
...  
Keyword(s):  
Microbial Transformation ◽  
Fermentation Medium ◽  
Growth Conditions ◽  
Alcaligenes Faecalis ◽  
Bacterial Isolates ◽  
Bacterial Strains ◽  
Enhanced Production ◽  
Plant Chemical ◽  
Fermentation Media

Abstract Vanillin is the major component which is responsible for flavor and aroma of vanilla extract and is produced by 3 ways: natural extraction from vanilla plant, chemical synthesis and from microbial transformation. Current research was aimed to study bacterial production of vanillin from native natural sources including sewage and soil from industrial areas. The main objective was vanillin bio-production by isolating bacteria from these native sources. Also to adapt methodologies to improve vanillin production by optimized fermentation media and growth conditions. 47 soil and 13 sewage samples were collected from different industrial regions of Lahore, Gujranwala, Faisalabad and Kasur. 67.7% bacterial isolates produced vanillin and 32.3% were non-producers. From these 279 producers, 4 bacterial isolates selected as significant producers were; A3, A4, A7 and A10. These isolates were identified by ribotyping as A3 Pseudomonas fluorescence (KF408302), A4 Enterococcus faecium (KT356807), A7 Alcaligenes faecalis (MW422815) and A10 Bacillus subtilis (KT962919). Vanillin producers were further tested for improved production of vanillin and were grown in different fermentation media under optimized growth conditions for enhanced production of vanillin. The fermentation media (FM) were; clove oil based, rice bran waste (residues oil) based, wheat bran based and modified isoeugenol based. In FM5, FM21, FM22, FM23, FM24, FM30, FM31, FM32, FM34, FM35, FM36, and FM37, the selected 4 bacterial strains produced significant amounts of vanillin. A10 B. subtilis produced maximum amount of vanillin. This strain produced 17.3 g/L vanillin in FM36. Cost of this fermentation medium 36 was 131.5 rupees/L. This fermentation medium was modified isoeugenol based medium with 1% of isoeugenol and 2.5 g/L soybean meal. ech gene was amplified in A3 P. fluorescence using ech specific primers. As vanillin use as flavor has increased tremendously, the bioproduction of vanillin must be focused.

scholarly journals Detection of aerobic bacterial pathogens associated with early embryonic death in pregnant New Zealand female Rabbits in Egypt

2021 ◽  
Vol 14 (4) ◽  
pp. 986-995
Author(s):  
Heba Roshdy ◽  
Azhar G. Shalaby ◽  
Ahmed Abd Elhalem Mohamed ◽  
Heba Badr
Keyword(s):  
Antibiotic Resistance ◽  
Pasteurella Multocida ◽  
Bacterial Pathogens ◽  
Bacterial Species ◽  
Bacterial Pathogen ◽  
Conventional Polymerase Chain Reaction ◽  
Bacterial Strains ◽  
Antibiotic Resistance Profile ◽  
Stx1 Gene ◽  
Embryonic Death

Background and Aim: Rabbits are a highly sensitive species and susceptible to various bacterial pathogens that may be causative agents for early embryonic death. This study aimed to explore the administration of different bacterial agents in does suffering from early embryonic death. Furthermore, identification of genes associated with virulence was performed to identify the phenotypic and genotypic antimicrobial resistance patterns that may increase the virulence of pathogens and lead to early embryonic death. Materials and Methods: We isolated and identified bacterial agents in 106 samples from live and dead female rabbits that had undergone early embryonic death, including liver and intestine tissue, aborted fetuses, discharges, and vaginal swabs. Conventional polymerase chain reaction (PCR) was conducted to confirm the identity of the isolated bacterial strains and their virulence. Moreover, antibiotic resistance was studied phenotypically and genotypically. Results: We isolated Escherichia coli, Salmonella, Staphylococcus aureus, Pasteurella multocida, and Listeria monocytogenes. PCR confirmed typical identification except in P. multocida, which was confirmed as Gallibacterium spp. in some cases. The final percentage of detection was 34%, 30.2%, 16.9%, 13.2%, and 11.3%, respectively. Virulence properties were investigated using different designated genes. All Salmonella strains harbored invA, stn, avrA, and ompf genes, while the sopE gene was identified in 31.25%. E. coli strains harboring the iss gene lacked the shiga toxin (stx1) gene. L. monocytogenes and S. aureus strains harbored the hemolysin gene (66.7% and 33.4%, respectively). Multidrug resistance was detected phenotypically and genotypically in most strains. Each bacterial pathogen had a different antibiotic resistance profile. Conclusion: Multiple bacterial species may contribute to early embryonic death in does. Furthermore, the combined infection could be the main cause of early embryonic death. Thus, monitoring programs should bear this in mind and focus on the early detection of these bacterial agents in female rabbits to avoid embryonic death.

scholarly journals Antibacterial activity of seneciolactone isolated from Senecio scandens against some common gastrointestinal tract disease causing bacterial pathogens

2016 ◽  
Vol 11 (1) ◽  
pp. 218
Author(s):  
Yi Zheng ◽  
Yu Wang ◽  
Yuan-Hong Shang
Keyword(s):  
Antibacterial Activity ◽  
Gastrointestinal Tract ◽  
Bacterial Pathogens ◽  
Cellular Membrane ◽  
Shigella Dysenteriae ◽  
Cellular Morphology ◽  
Bacterial Cells ◽  
Bacterial Strains ◽  
Zones Of Inhibition ◽  
Tract Disease

<p class="Abstract">The objective of the present investigation was to study the antibacterial effect of seneciolactone isolated from the methanolic extract of <em>Senecio scandens</em> against five bacterial pathogens which are known to cause several gastrointestinal tract diseases. Disc diffusion assay and agar well diffusion assays were used to examine the antibacterial efficacy of this compound by measuring zones of inhibition and MIC/MBC values. Scanning electron microscopy (SEM) was involved to study the effect of this compound on cellular morphology of <em>Shigella dysenteriae</em>. Results revealed that seneciolactone exhibited moderate to potent antibacterial activity against different bacterial strains. Zones of inhibition and MIC/MBC values indicated that seneciolactone was most potent against<em> S. dysenteriae</em> followed by <em>Pseudomonas aeruginosa</em> and <em>E. coli</em>. SEM results indicated that seneciolactone induced potent damage to the cell membrane of the tested bacteria. As compared to the untreated control which exhibited normal cellular morphology, the seneciolactone treated bacterial cells revealed severe damage to the cellular membrane particularly at the higher doses.</p><p> </p>

scholarly journals Synthesis of Silver Nano particles by Green method Using Vernonia anthelmintica and its Antibacterial activity.

2021 ◽  
Vol 23 (06) ◽  
pp. 1396-1406
Author(s):  
Tarannum Rashid Patave ◽  
◽  
Dr. Aquil-ur Rahim Siddiqui ◽  
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Bacterial Infections ◽  
Color Change ◽  
Research Work ◽  
Atomic Emission ◽  
Colloidal Dispersion ◽  
Nano Particles ◽  
Multiple Drug ◽  
Green Method

In the modern years, there is an enlarged use of silver nanoparticles (SNPs) in industrial and in the field of medical practice significantly, still, their high risk and lethal outcome have not been studied considerably. The aim behind this research work was to incorporate silver nanoparticles (AgNPs) using Vernonia anthelmintic seeds extract and to assess its antibacterial activity. AgNPs were properly synthesized by the green method and the evolution of AgNPs was firmed by optical color change and UV (ultraviolet) spectroscopy. Synthesized AgNPs were decontaminated and characterized by using Silver Content Estimation-Inductive Coupled Plasma-Atomic Emission Spectroscopy (ICP-AES), Zeta potentiometry, X-ray diffraction (XRD), and transmission electron microscopy (TEM). UV peak at 453.5 nm confirmed the formation of AgNPs. XRD studies disclosed the crystalline nature of AgNPs. ICP-AES studies showed the presence of silver in colloidal dispersion and images were recorded by using HRTEM. Synthesized AgNPs were found to be potent against Staphylococcus aureus, Pseudomonasaeruginosa, E. coli, and MRSA. However, these SNPs are cytotoxic in nature and could obey as a superior antibacterial for therapy of bacterial infections in this period of multiple drug defiance.

COMPARATIVE IN VITRO ANTIBACTERIAL ACTIVITY OF ETHANOLIC EXTRACT OF PLUMBAGO ZEYLENICA

INDIAN DRUGS ◽  
2013 ◽  
Vol 50 (04) ◽  
pp. 53-55
Author(s):  
G.P Vadnere ◽  
◽  
A.R. Pathan ◽  
B.U Kulkarni ◽  
A.K. Singhai
Keyword(s):  
Antibacterial Activity ◽  
Bacterial Pathogens ◽  
Bacterial Pathogen ◽  
Ethanolic Extract ◽  
Salmonella Typhi ◽  
Gastrointestinal Disorders ◽  
Moderate Activity ◽  
White Variety ◽  
In Vitro Antibacterial Activity

Ethanolic extract of leaf, flower and root of Plumbago zeylenica L. (white variety) traditionally used in the treatment of gastrointestinal disorders were screened for antibacterial activity against bacterial pathogens Staphyllococcus aureus, Escerchia coli, Klebsiella pneumoniae, Enterobacter aurugens, Proteus vulgaris, Shigella sonnei, Salmonella typhi which are known to cause different type of gastrointestinal disorders. The results showed that leaf and root extracts exhibited strong antibacterial action at concentration of 2-8 mg/ mL of the bacterial pathogen tested. However, powdered flower extract showed moderate activity. Thus the present data reveals that leaves and flowers are equally potential for the treatment of bacterial pathogens, which causes intestinal catarrh, indigestion, and colic intestinal abscesses.

Extraction and characterization of sea anemones compound and its Anti bacterial and hemolytic studies

2020 ◽  
Vol 10 (4) ◽  
pp. 93-97
Author(s):  
Anil Kumar A ◽  
Raja Sheker K ◽  
Naveen B ◽  
Abhilash G ◽  
Akila CR
Keyword(s):  
Antibacterial Activity ◽  
Focal Point ◽  
Haemolytic Activity ◽  
Klebsiella Pneumonia ◽  
Bacterial Strains ◽  
Sea Anemones ◽  
Marine Plants ◽  
Heteractis Magnifica ◽  
Trididemnum Solidum

Seas assets that give us a variety of characteristic items to control bacterial, contagious and viral ailment and mostly utilized for malignancy chemotherapy practically from spineless creatures, for example, bryozoans, wipes, delicate corals, coelenterates, ocean fans, ocean bunnies, molluscs and echinoderms. In the previous 30 - 40 years, marine plants and creatures have been the focal point of overall endeavours to characterize the regular results of the marine condition. Numerous marine characteristic items have been effectively exceptional to the last phases of clinical preliminaries, including dolastatin-10, a group of peptides disengaged from Indian ocean rabbit, Dollabella auricularia. Ecteinascidin-743 from mangrove tunicate Ecteinascidia turbinata, Didemnins was isolated from Caribbean tunicate Trididemnum solidum and Conopeptides from cone snails (Conus sp.), and a developing number of up-and-comers have been chosen as promising leads for expanded pre-clinical appraisals. Sea anemones possess numerous tentacles containing stinging cells or cnidocytes. The stinging cells are equipped with small organelles known as nematocysts. The two species of sea anemones namely, Heteractis magnificaandStichodactyla haddoni, were collected from Mandapam coastal waters of Ramanathapuram district, Tamilnadu, India. The Nematocyst was collected and centrifuged, and the supernatant was lyophilized and stored for further analysis. The amount of protein from Heteractis Magnifica and Stichodactyla haddoni was estimated. The crude extract has shown haemolytic activity on chicken blood and goat blood. In the antibacterial activity of the sea anemone against six bacterial strains Staphylococcus aureus, Salmonella typhii, Salmonella paratyphii, Klebsiella pneumonia, Vibrio cholerae, Pseudomonas aeruginosa. Antibacterial activity of H. Magnifica and S.haddoni was measured as the radius of the zone of inhibition.

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