scholarly journals Phytochemical Analysis of Fagonia schweinfurthii Hadidi

2021 ◽  
pp. 803-811
Author(s):  
Yamini Joshi ◽  
B. K. Jain
Keyword(s):  
High Performance ◽  
Skin Diseases ◽  
Chemical Components ◽  
Phytochemical Analysis ◽  
Root Extract ◽  
Chromatography Method ◽  
New Era ◽  
Desert Region ◽  
Plant Parts ◽  
Methanolic Extracts

Fagonia schweinfurthii Hadidi (family zygophyllaceae) is plant of desert region .In Gujarat it is found on the coastal area of Dwarka, Bet Dwarka and Rann of Kachcha. It is locally called dhamasia or dhamaso. Plants parts including leaves, roots, rhizomes, stems, barks, flowers, fruits, grains or seeds which can be employed in the control or treatment of infectious disease which causes damage to the respiratory system, urinary tract, gastrointestinal and bilary systems and on the skin. The plant parts contain chemical components that are medically active. Tribal people living in desert region use this plant to cure number of ailments such as skin eruptions, skin diseases, anti- pyretic, in pain relief, in heal sores, ear infection, venereal diseases etc. In the new era of science and technology there is increasing need to validate the claims of traditional knowledge database for safe, easily available, cheap, side- effect free healthcare provisions. In the present study preliminary phytochemical analysis of plant parts of Fagonia schweinfurthii Hadidi was done by the standard method of Sofowora, 1982; Trease & Evans, 1989 method, which confirms the presence of alkaloids, tannin, saponin, terpinoid and steroids. High Performance Thin Layer Chromatography method was developed using mobile phase containing Chloroform: methanol: acetic acid in ratio 70:30:0.2 for detecting phyto-active constituents from the methanolic extracts of the leaf, stem and root of Fagonia schweinfurthii Hadidi. Methanolic extract of Fagonia schweinfurthii Hadidi of leaf, stem, fruit and root detected 8, 8, 7 and 4 numbers of peaks at 254 nm and at 366 nm 10, 12,  13 and 12 numbers of peaks from leaf, stem, fruit and root extract of Fagonia schweinfurthii Hadidi were detected.

scholarly journals Extracts from Pulsatilla patens target cancer-related signaling pathways in HeLa cells

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Grażyna Łaska ◽  
Magdalena Maciejewska-Turska ◽  
Elwira Sieniawska ◽  
Łukasz Świątek ◽  
David S. Pasco ◽  
...  
Keyword(s):  
Signaling Pathways ◽  
Hela Cells ◽  
Luciferase Reporter ◽  
Cervical Cancer Cell Line ◽  
Phytochemical Analysis ◽  
Root Extract ◽  
Triterpenoid Saponins ◽  
Cancer Signaling ◽  
Methanolic Extracts ◽  
Pulsatilla Patens

AbstractThe purpose of this study was to determine if a methanolic extract of the Pulsatilla patens (L.) Mill. can inhibit the progression of cancer through the modulation of cancer-related metabolic signaling pathways. We analyzed a panel of 13 inducible luciferase reporter gene vectors which expression is driven by enhancer elements that bind to specific transcription factors for the evaluation of the activity of cancer signaling pathways. The root extract of P. patens exhibited strong inhibition of several signaling pathways in HeLa cells, a cervical cancer cell line, and was found to be the most potent in inhibiting the activation of Stat3, Smad, AP-1, NF-κB, MYC, Ets, Wnt and Hdghog, at a concentration of 40 µg/mL. The methanolic extracts of P. patens enhanced apoptotic death, deregulated cellular proliferation, differentiation, and progression towards the neoplastic phenotype by altering key signaling molecules required for cell cycle progression. This is the first study to report the influence of Pulsatilla species on cancer signaling pathways. Further, our detailed phytochemical analysis of the methanolic extracts of the P. patens allowed to deduce that compounds, which strongly suppressed the growth and proliferation of HeLa cancer cells were mainly triterpenoid saponins accompanied by phenolic acids.

scholarly journals Determination of Ten Corticosteroids in Illegal Cosmetic Products by a Simple, Rapid, and High-Performance LC-MS/MS Method

2017 ◽  
Vol 2017 ◽  
pp. 1-12 ◽  
Author(s):  
Vita Giaccone ◽  
Giuseppe Polizzotto ◽  
Andrea Macaluso ◽  
Gaetano Cammilleri ◽  
Vincenzo Ferrantelli
Keyword(s):  
High Performance ◽  
Skin Diseases ◽  
Gradient Elution ◽  
Reversed Phase ◽  
Cosmetic Products ◽  
Chromatography Method ◽  
Esi Ms ◽  
Negative Ionization Mode ◽  
Negative Ionization

The aim of our present work was the development of a rapid high-performance liquid chromatography method with electrospray ionization and tandem mass spectrometry detection (LC-ESI-MS/MS) for the determination of several corticosteroids in cosmetic products. Corticosteroids are suspected to be illegally added in cosmetic preparations in order to enhance the curative effect against some skin diseases. Sample preparation step consists in a single extraction with acetonitrile followed by centrifugation and filtration. The compounds were separated by reversed-phase chromatography with water and acetonitrile (both with 0.1% formic acid) gradient elution and detected by ESI-MS positive and negative ionization mode. The method was validated at the validation level of 0.1 mg kg−1. Linearity was studied in the 5–250 μg L−1 range and linear coefficients (r2) were all over 0.99. The accuracy and precision of the method were satisfactory. The LOD ranged from 0.085 to 0.109 mg kg−1 and the LOQ from 0.102 to 0.121 mg kg−1. Mean recoveries for all the analytes were within the range 91.9–99.2%. The developed method is sensitive and useful for detection, quantification, and confirmation of these corticosteroids in cosmetic preparations and can be applied in the analysis of the suspected samples under investigation.

scholarly journals HPTLC METHOD FOR ESTIMATION AND QUANTIFICATION OF β-SITOSTEROL FROM IN-VIVO AND IN-VITRO SAMPLES OF MERREMIA AEGYPTIA AND MERREMIA DISSECTA

2020 ◽  
pp. 162-165
Author(s):  
RIDHI JOSHI ◽  
RISHIKESH MEENA ◽  
PREETI MISHRA ◽  
VIDYA PATNI
Keyword(s):  
High Performance ◽  
Normal Phase ◽  
Leaf Sample ◽  
Plant Parts ◽  
Methanolic Extracts ◽  
Aluminum Plates ◽  
Layer Chromatography ◽  
Hptlc Method

Objective: A normal-phase high-performance thin-layer chromatography (HPTLC) method has been developed and validated for estimation and quantitation of beta-sitosterol from the methanolic fraction of different plant parts of two medicinally important plants viz. Merremia aegyptia and Merremia dissecta. These plants have been reported to possess antimicrobial, antioxidant, and anti-inflammatory activities. Methods: Chromatographic separation of beta-sitosterol from the methanolic extracts of plant parts of M. aegyptia and M. dissecta was performed on TLC aluminum plates pre-coated with silica gel 60F254 using a suitable mobile phase. The densitometric scanning was done after derivatization at ????-580 nm for ????-sitosterol. Result: Only M. dissecta leaf sample was reported to contain ????-sitosterol (4.6 ng/μl), whereas other samples such as seed, stem, and callus extracts of M. aegyptia and M. dissecta did not showed its presence. Conclusion: The developed HPTLC method is simple, rapid, and precise and can be used for routine analysis and quantification of ????-sitosterol and other useful plant bioactives that are phytopharmaceutically important.

Effect of Gut Microbiota on the Metabolism of Chemical Constituents of Berberis kansuensis Extract Based on UHPLC-Orbitrap-MS Technique

Planta Medica ◽  
2021 ◽  
Author(s):  
Huan Du ◽  
Tong Xu ◽  
Huan Yi ◽  
Xinmei Xu ◽  
Chengcheng Zhao ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
High Performance ◽  
Chemical Constituents ◽  
Chemical Components ◽  
Serum Samples ◽  
Chromatography Method ◽  
Metabolic Transformation ◽  
Orbitrap Ms

AbstractThe dried stem bark of Berberis kansuensis is a commonly used Tibetan herbal medicine for the treatment of diabetes. Its main chemical components are alkaloids, such as berberine, magnoflorine and jatrorrhizine. However, the role of gut microbiota in the in vivo metabolism of these chemical components has not been fully elucidated. In this study, an ultra-high performance liquid chromatography method coupled with Orbitrap mass spectrometry (UHPLC-Orbitrap-MS) technology was applied to detect and identify prototype components and metabolites in rat intestinal contents and serum samples after oral administration of a B. kansuensis extract. A total of 16 prototype components and 40 metabolites were identified. The primary metabolic pathways of the chemical components from B. kansuensis extract were demethylation, desaturation, deglycosylation, reduction, hydroxylation, and other conjugation reactions including sulfation, glucuronidation, glycosidation, and methylation. By comparing the differences of metabolites between diabetic and pseudo-germ-free diabetic rats, we found that the metabolic transformation of some chemical components in B. kansuensis extract such as bufotenin, ferulic acid 4-O-β-D-glucopyranoside, magnoflorine, and 8-oxyberberine, was affected by the gut microbiota. The results revealed that the gut microbiota can affect the metabolic transformation of chemical constituents in B. kansuensis extract. These findings can enhance our understanding of the active ingredients of B. kansuensis extract and the key role of the gut microbiota on them.

scholarly journals DEVELOPMENT OF HIGH PERFORMANCE THIN LAYER CHROMATOGRAPHY METHOD FOR QUANTITATION OF ?-SITOSTEROL IN NORMACID SYRUP: A POLY HERBAL FORMULATION

2021 ◽  
Vol 5 (2) ◽  
Author(s):  
Shah Kinjal H ◽  
Makwana Rajeshree
Keyword(s):  
High Performance ◽  
Control Method ◽  
Quantitative Estimation ◽  
Chromatography Method ◽  
Plant Parts ◽  
Herbal Formulation ◽  
Quality Control Method ◽  
Layer Chromatography ◽  
Hptlc Method ◽  
Nanogram Level

Normacid syrup consisiting of 17 ingredients, used in the treatment of hyperacidity and gastritis. ?- Sitosterol is one of the phytoconstituent present in Normacid syrup- a poly herbal formulation. In the present study an attempt has been made to develop a HPTLC method for quantitative estimation of ?- Sitosterol in dried plant parts used in formulations. This HPTLC method was found to be reproducible, accurate, precise and detect ?- Sitosterol at nanogram level. The developed HPTLC method would be an important tool in the quality control method of poly herbal formulation.  Keywords: HPTLC, Polyherbal, ?- Sitosterol, Gastritis

scholarly journals Phytochemical Analysis of Muntingia calabura Extracts Possessing Anti-Microbial and Anti-Fouling Activities

2017 ◽  
Vol 9 (6) ◽  
Author(s):  
Singh R. ◽  
Iye S. ◽  
Prasad S. ◽  
Deshmukh N. ◽  
Gupta U. ◽  
...  
Keyword(s):  
Traditional Medicine ◽  
Microbial Activity ◽  
Plant Extracts ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Hemagglutination Activity ◽  
Standard Drug ◽  
Plant Parts ◽  
Methanolic Extracts ◽  
Muntingia Calabura

The present study was carried out to determine the possible antifouling and anti microbial activity of plant extracts prepared from Muntingia calabura and phytochemical screening was demonstrated. The extracts from the following plant parts like stem, leaves, fruits, flower and stems were prepared in different aqueous solvents like ethanol, methanol, acetone, acetonitrile and water. The highest antimicrobial potentials were observed for the methanolic extracts against K. pneumonia, B. subtilis, B. megaterium and P. aeruginosa. Its efficacy was comparable to the standard drug, ampicillin. Significant amount of tannins, alkaloids, steroids and flavonoids were found. Interestingly, the present study showed anti fouling effect against pathogenic biofilm forming bacteria. These extracts also contain high hemagglutination activity. The present study provides evidence that solvent extract of M. calabura contains medicinally important bioactive compounds and this justifies the use of plant species as traditional medicine for treatment of various diseases.

Qualitative and Quantitative Phytochemical Screening and Chemical Fingerprint Analysis of Herbal Plant Phyllanthus niruri using HPTLC

2021 ◽  
Vol 13 (2) ◽  
pp. 623-633
Author(s):  
R. Nigam ◽  
R. Arnold
Keyword(s):  
High Performance ◽  
Ethanol Extract ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Root Extract ◽  
Phytochemical Screening ◽  
Phyllanthus Niruri ◽  
Phenolic Contents ◽  
Stem Extract ◽  
Herbal Plant

  This study focused to develop the fingerprint profile of Phyllanthus niruri through high performance thin layer chromatography. Various solvent extracts such as ethanol and water were prepared and phytochemical screening was made using standard procedures. The preliminary phytochemical analysis of two extracts of the plant showed the presence of secondary metabolites viz., alkaloids, triterpenoids, proteins, amino acids, flavonoids and steroids. The total flavonoid contents of leaves were significantly higher than those revealed in stem and root. The total phenolic contents of Phyllanthus niruri leaves (aqueous extract), leaves (ethanol extract) and root (ethanol extract) were 5.71 mg GAE/100 mg extract, 7.66 mg GAE/100 mg and 5.63 mg GAE/100 mg extract respectively. Methanol extract of Phyllanthus niruri was subjected to HPTLC analysis due to it is most effective, highly polar universal solvent for extraction of phytoconstituents. Among the samples, leaves extract resolved maximum number of spots followed by stem extract and root extract. It can be concluded that HPTLC fingerprinting of Phyllanthus niruri may be useful in differentiating the species from the adulterant and authentication of this herbal plant in the medical field and systematic plant studies.

scholarly journals Histochemical and Phytochemical Analysis of Medicinally Important Plants

2020 ◽  
pp. 1-13
Author(s):  
B. K. Sushma ◽  
K. S. Ashalatha ◽  
Preetam Ray ◽  
H. R. Raveesha
Keyword(s):  
Phytochemical Analysis ◽  
Root Extract ◽  
Vascular Bundles ◽  
Bioactive Constituents ◽  
Leaf Extracts ◽  
Methanolic Extracts ◽  
Isolation And Characterization ◽  
Phytochemical Studies ◽  
Maximum Content

The present study was aimed to investigate the histochemical and phytochemical studies of four important medicinal plants. Leaf, stem and root sections were examined for alkaloid, protein and phenolic tests. Methanolic extracts of leaf, stem and root were analysed for the phytochemical screening of major bioactive constituents. Histochemical analysis reveals the presence of alkaloids, phenols and proteins in epidermis, cortex and vascular bundles of root, stem and leaf. Preliminary phytochemical analysis showed the presence of alkaloids, tannins, phenols, flavonoids, steroids, saponins, terpenoids, coumarins, quinone and proteins. The root extract of Catharanthus roseus recorded maximum content of phenols compared to other extracts. Costus pictus root and leaf extracts exhibited a significant amount of flavonoids and tannins respectively. Further studies were focused on isolation and characterization of each plant compounds.

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