A Round Trip to the Desert: In situ Nanopore Sequencing Informs Targeted Bioprospecting

Bioprospecting expeditions are often performed in remote locations, in order to access previously unexplored samples. Nevertheless, the actual potential of those samples is only assessed once scientists are back in the laboratory, where a time-consuming screening must take place. This work evaluates the suitability of using Nanopore sequencing during a journey to the Tabernas Desert (Spain) for forecasting the potential of specific samples in terms of bacterial diversity and prevalence of radiation- and desiccation-resistant taxa, which were the target of the bioprospecting activities. Samples collected during the first day were analyzed through 16S rRNA gene sequencing using a mobile laboratory. Results enabled the identification of locations showing the greatest and the least potential, and a second, informed sampling was performed focusing on those sites. After finishing the expedition, a culture collection of 166 strains belonging to 50 different genera was established. Overall, Nanopore and culturing data correlated well, since samples holding a greater potential at the microbiome level also yielded a more interesting set of microbial isolates, whereas samples showing less biodiversity resulted in a reduced (and redundant) set of culturable bacteria. Thus, we anticipate that portable sequencers hold potential as key, easy-to-use tools for in situ-informed bioprospecting strategies.

Combined Building-Energy Systems with Heat Transfer Control by Building Constructions using RES

Energy systems built into one of the building structures that serve to capture solar energy, geothermic energy, and ambient energy, or which have the function of end elements of heating, cooling, and ventilation system, we generally call combined building-energy systems. Among combined building-energy systems we include solar roofs with built-in pipe absorbers, building structures with active thermal protection (ATP) - active heat transfer control, which have a multifunctional purpose – a thermal barrier, low-temperature heating, high-temperature cooling, recuperation and accumulation of heat, solar and ambient energy collection, large-capacity heat storage (ground heat accumulators built simultaneously in the foundation slab of the building), or heat exchangers used for recuperative ventilation of buildings built into the foundation slabs and wall structures. The research of combined building-energy systems at the Department of Building Services, Faculty of Civil Engineering, Slovak University of Technology in Bratislava has been carried out continuously since 2005. Within five research projects (responsible researcher, Kalús, D.) HZ 04-309-05, HZ 04-310- 05, HZ 04-142-07 (research and experimental measurements took place in the years 2005 to 2007), HZ PG73/2011 (research and experimental measurements took place in the years 2011 to 2013), [13,] and HZ PR10/2015 (research and experimental measurements have been carried out since 2015), two experimental houses IDA I. and EB2020, a mobile laboratory designed for measuring and optimizing a compact heat station using renewable heat sources, were designed and built by the research team at our workplace, and also a research of a fragment of a perimeter wall with built-in active thermal protection was carried out in the climatic chamber of the Faculty of Civil Engineering STU in Bratislava, Slovak Republic. Significant contribution to the research was provided by doctoral students Ing. Martin Cvíčela, Ph.D., (supervisor, Kalús, D.), Ing. Peter Janik, PhD., (supervisor, Kalús, D.) and Ing. Martin Šimko, PhD., (supervisor, Kalús, D.), who described the results of the research in their dissertations. At present experimental measurements in the mobile laboratory are performed by doctoral student Ing. Matej Kubica, (supervisor, Kalús, D.). In the area of combined construction and energy systems, research and optimization of suitable solutions continues, which have been transformed into one European patent and three utility models.

Improving motivation and science process skills through a mobile laboratory-based learning model

The COVID-19 pandemic has affected all sectors of life, including the education sector. Physics learning activities, especially practicum activities, which so far have been very dependent on laboratory space-based activities, are very disturbed. This study aims to develop students' motivation and science process skills (SPS) during the pandemic. The sample of this study was 3 groups consisting of 90 students from 2 universities in the province of Lampung, Indonesia. The data obtained were analysed using the multivariate analysis of variance technique. The results showed that mobile laboratory-based learning proved effective in developing students' motivation and SPS. Therefore, experimental/mobile laboratory activities based on analytical videos obtained from surrounding physical phenomena can be an alternative for physics learning activities, especially in the current era of distance learning. Keywords: Mobile laboratory, motivation, science process skills.

Application of a mobile laboratory using a selected-ion flow-tube mass spectrometer (SIFT-MS) for characterisation of volatile organic compounds and atmospheric trace gases

Over the last 2 decades, the importance of emissions source types of atmospheric pollutants in urban areas has undergone significant change. In particular, there has been a considerable reduction in emissions associated with road vehicles. Understanding the role played by different source sectors is important if effective air pollution control is to be achieved. Current atmospheric measurements are made at fixed monitoring sites, most of which do not include the measurement of volatile organic compounds (VOCs), so our understanding of the temporal and spatial variation of pollutants is limited. Here we describe the application of a mobile laboratory using a selected-ion flow-tube mass spectrometer (SIFT-MS) and other trace gas instrumentation to provide on-road, high-spatial- and temporal-resolution measurements of CO2, CH4, VOCs and other trace gases. We then illustrate the potential of this platform for developing source characterisation methods that account for the similarity in correlation between species. Finally, we consider the benefits of high-spatial- and temporal-resolution measurements in characterising different types of sources, which would be difficult or impossible for single-species studies.

Telecommunication Facilities, Key Support for Data Management and Data Sharing by a Biological Mobile Laboratory Deployed to Counter Emerging Biological Threats and Improve Public Health Crisis Preparedness

In the case of rapid outbreaks of infectious diseases in remote locations, the lack of real-time information from the field and rapid spread of misinformation can be a major issue. To improve situational awareness and decision-making at all levels of operational deployment, there is an urgent need for accurate, reliable, and timely results from patients from the affected area. This requires a robust and fast channel of communication connecting first responders on-site, crisis managers, decision-makers, and the institutions involved in the survey of the crisis at national, regional, and international levels. This has been the rationale sustaining the development of advanced communication tools in the Biological Light Fieldable Laboratory for Emergencies (B-LiFE). The benefit of terrestrial (TETRA, LTE, 5G, and Wi-Fi-Fi) and SatCom communications is illustrated through a series of missions and exercises conducted in the previous five years. These tools were used by B-LiFE operators to provide accurate, comprehensive, timely, and relevant information and services in real time. The focus of this article is to discuss the development and benefits of the integration of multi-mission, multi-user nomadic, rapidly deployable telecommunication nodes for emergency uses (TEN) in the capacity of B-LiFE. Providing reliable communication channels through TEN enables the development and use of an ICT toolbox called MIML_LIMS (multi-institution, multi-mission, multi-laboratory LIMS), a tool which is mandatory for efficient and secure data management and data sharing by a mobile laboratory.

A Fast Method for Estimating the Emission Factors of Air Pollutants from In-Use Vehicles Fleet

The real-world emission factors of gaseous and particulate air pollutants emitted from in-use vehicles, can be rapidly estimated using monitoring data of their concentration profiles from inside roadway tunnels using a mobile laboratory equipped with fast monitoring instruments. The concentrations of CO2 and particle-bound polycyclic aromatic hydrocarbons (PM-PAHs) and NOx, were observed to increase linearly with traveling distance inside two successive roadway tunnels: the Hongjimun Tunnel and the Jeongneung Tunnel on the Naebu Express Way in Seoul, Korea, except for a small region of decrease. In the decreasing regions, within a few hundred meters of the entrance and before the exit, outside background air with low concentrations of air pollutants was thought to have intruded. From the slopes of the linear regression between distance and concentrations, a fleet-averaged (light-, medium-, and heavy-duty vehicles with 54%, 36%, and 10%, respectively) emission factor of CO2, PM-PAHs, and NOx at an average speed of ~60 km h−1 could be calculated as 197 ± 38 g km−1, 4.2 ± 0.8 × 10−4 g km−1, and 0.530 ± 0.230 g km−1, respectively, which are within the ranges of values reported in the literature. For each tunnel, the emission factors of CO2, PM-PAHs, and NOx estimated on days with higher-than-normal fractions of heavy-duty vehicles, were higher than those on other days. From these results, the new fast method proposed in this study is considered useful for estimating real-world emission factors of air pollutants by using a mobile laboratory as a complementary tool to traditional tunnel studies. This method can be used to rapidly make emission maps at roadway tunnels in mega-cities like Seoul, Korea, for urban air-quality management.

Integrated Mobile Laboratory for Air Pollution Assessment: Literature Review and cc-TrAIRer Design

To promote research studies on air pollution and climate change, the mobile laboratory cc-TrAIRer (Climate Change—TRailer for AIR and Environmental Research) was designed and built. It consists of a trailer which affords particles, gas, meteorological and noise measurements. Thanks to its structure and its versatility, it can easily conduct field campaigns in remote areas. The literature review presented in this paper shows the main characteristics of the existing mobile laboratories. The cc-TrAIRer was built by evaluating technical aspects, instrumentations and auxiliary systems that emerged from previous studies in the literature. Some of the studies conducted in heterogeneous topography areas, such as the Po Valley and the Alps, using instruments that were chosen to be located on the mobile laboratory are here reported. The preliminary results highlight the future applications of the trailer and the importance of high temporal resolution data acquisition for the characterization of pollution phenomena. The potential applications of the cc-TrAIRer concern different fields, such as complex terrain, emergency situations, worksite and local source impacts and temporal and spatial distributions of atmospheric compounds. The integrated use of gas and particle analysers, a weather station and environment monitoring systems in a single easily transportable vehicle will contribute to research studies on global aspects of climate change.

The East African Community (EAC) mobile laboratory networks in Kenya, Burundi, Tanzania, Rwanda, Uganda, and South Sudan—from project implementation to outbreak response against Dengue, Ebola, COVID-19, and epidemic-prone diseases

Background East Africa is home to 170 million people and prone to frequent outbreaks of viral haemorrhagic fevers and various bacterial diseases. A major challenge is that epidemics mostly happen in remote areas, where infrastructure for Biosecurity Level (BSL) 3/4 laboratory capacity is not available. As samples have to be transported from the outbreak area to the National Public Health Laboratories (NPHL) in the capitals or even flown to international reference centres, diagnosis is significantly delayed and epidemics emerge. Main text The East African Community (EAC), an intergovernmental body of Burundi, Rwanda, Tanzania, Kenya, Uganda, and South Sudan, received 10 million € funding from the German Development Bank (KfW) to establish BSL3/4 capacity in the region. Between 2017 and 2020, the EAC in collaboration with the Bernhard-Nocht-Institute for Tropical Medicine (Germany) and the Partner Countries’ Ministries of Health and their respective NPHLs, established a regional network of nine mobile BSL3/4 laboratories. These rapidly deployable laboratories allowed the region to reduce sample turn-around-time (from days to an average of 8h) at the centre of the outbreak and rapidly respond to epidemics. In the present article, the approach for implementing such a regional project is outlined and five major aspects (including recommendations) are described: (i) the overall project coordination activities through the EAC Secretariat and the Partner States, (ii) procurement of equipment, (iii) the established laboratory setup and diagnostic panels, (iv) regional training activities and capacity building of various stakeholders and (v) completed and ongoing field missions. The latter includes an EAC/WHO field simulation exercise that was conducted on the border between Tanzania and Kenya in June 2019, the support in molecular diagnosis during the Tanzanian Dengue outbreak in 2019, the participation in the Ugandan National Ebola response activities in Kisoro district along the Uganda/DRC border in Oct/Nov 2019 and the deployments of the laboratories to assist in SARS-CoV-2 diagnostics throughout the region since early 2020. Conclusions The established EAC mobile laboratory network allows accurate and timely diagnosis of BSL3/4 pathogens in all East African countries, important for individual patient management and to effectively contain the spread of epidemic-prone diseases.