CADAVERIC STUDY OF KATIKTARUN MARMA WITH DETAILED DESCRIPTION OF ITS LOCATION AND AGHAT LAKSHANS

Marma Science is one of the most distinctive concepts of Ayurveda. There are 107 marma sites in the body, and they are the conglomeration of muscles, veins, ligaments, bones, and joints. This peculiarity makes Marmamarma a somewhat vulnerable point, and any injury can lead to disability, dysfunction and demise. The cause of the damage can either be traumatic or iatrogenic; therefore, it becomes a necessity to rule out the exact location of the marma and anatomical structure responsible for the traumatic effects. Katiktarun being a Prishthagata marma, is prone to get injured during significant surgeries of the gluteal region and spine. Its injury can lead to delayed death. The aim of this study revolves around the anatomical entity responsible for delayed death caused by katiktarun injury. By identifying the location and structure involved in the marma, it might be possible to repair the structure and deferment the delayed end. Based on Ayurvedic literature and cadaveric observations, the superior margin of the sciatic notch (suprapiriform foraman) is considered as the position of Katiktarun Marma, whereas the neurovasculature associated with suprapiriform foramen is the causative structure of marma trauma symptoms.

A Novel Reference for Bt-Resistance Mechanism in Plutella xylostella Based on Analysis of the Midgut Transcriptomes

The diamondback moth, Plutella xylostella, is a lepidopteran insect that mainly harms cruciferous vegetables, with strong resistance to a variety of agrochemicals, including Bacillus thuringiensis (Bt) toxins. This study intended to screen genes associated with Bt resistance in P. xylostella by comparing the midgut transcriptome of Cry1Ac-susceptible and -resistant strains together with two toxin-treated strains 24 h before sampling. A total of 12 samples were analyzed by BGISEQ-500, and each sample obtained an average of 6.35 Gb data. Additionally, 3284 differentially expressed genes (DEGs) were identified in susceptible and resistant strains. Among them, five DEGs for cadherin, 14 for aminopeptidase, zero for alkaline phosphatase, 14 for ATP binding cassette transport, and five heat shock proteins were potentially involved in resistance to Cry1Ac in P. xylostella. Furthermore, DEGs associated with “binding”, “catalytic activity”, “cellular process”, “metabolic process”, and “cellular anatomical entity” were more likely to be responsible for resistance to Bt toxin. Thus, together with other omics data, our results will offer prospective genes for the development of Bt resistance, thereby providing a brand new reference for revealing the resistance mechanism to Bt of P. xylostella.

Endurance exercise: a model of physiological integration

Endurance exercise is a model of physiological integration. There is no other animal activity in which cardiovascular, respiratory, metabolic-endocrine and neuromuscular functions are activated at the same time. Even apparently, silent functions are essential during exercise (digestive, renal). During long-term exercise, the absorption of water and carbohydrates is a determining factor in performance. Kidney function plays a fundamental role in trying to preserve the hydro-electrolyte balance during exercise. In this work we present an integrative physiological perspective during dynamic exercise (mobilization of a large muscle mass with a low to moderate degree of strength development), both from the point of view of health and performance. The response of the heart rate in the first moments of exercise is a good example of the feedforward mechanism. Overall, the nervous system has two control mechanisms: feedforward and feedback. These depend on the central command, a more functional than anatomical entity. The feedforward system allows to immediately start the cardiovascular and respiratory systems. This mechanism is important because it activates the organism to overcome resting state. The feedback system is equally important because it allows the central command to receive the necessary information to “order” the appropriate response according to the intensity of the exercise. The information for retrocontrol comes from various receptors located in: the muscles, the respiratory system and the cardiovascular system. It is complex information that the central nervous system processes with exquisite precision, as can be seen in in endurance exercise.

RNA-Seq analysis and transcriptome assembly of Salicornia neei reveals a powerful system for ammonium detoxification

ABSTRACTBackgroundSalicornia neei is a halophyte plant that has been proposed for phytoremediation of saline wastewater generated by land-based aquaculture, which usually contains elevated concentrations of ammonium resulting from protein metabolism. To identify the molecular mechanisms related to ammonium response through of analysis results in silico and the Michaelis–Menten ammonium removal biokinetics and the transcriptome of S. neei in response to growth in saline water containing 3 mM ammonium.ResultsThe parameters for ammonium uptake by S. neei root cuttings were estimated: 1) maximum uptake rate Imax = 7.07 ± 0.27 mM N g−1 fresh weight h−1; and 2) half-saturation constant Km = 0.85 ± 0.12 mM N L−1. Further, a total of 45,327 genes were annotated, which represents 51.2% of the contig predicted from de novo assembly. A total of 9,140 genes were differentially expressed in response to ammonium in saline water, but only 7,396 could be annotated against functional databases. According to the GO enrichment and as well as KEGG pathway analyses showed these upregulated genes were involved in pr cellular anatomical entity, cellular process, and metabolic process, including biological KEGG pathways linked to biosynthesis amino acid biosynthesis, nitrogen metabolism and autophagy and other. In addiction, a set of 72 genes were directly involved in ammonium metabolism, including glutamine synthetase 1 (GLN1), glutamate synthase 1 (GLT1), and ferredoxin-dependent glutamate synthase chloroplastic (Fd-GOGAT).ConclusionOur results support the hypothesis that an ammonium detoxification system mediated by glutamine and glutamate synthase was activated in S. neei when exposed to ammonium and saline water. These results provide novel insight into understanding the molecular mechanisms of ammonium nutrition and aid for investigating the response of halophyte plants to saline wastewater from land-based aquaculture

Anatomy, physiology, and pathology of body fat

Subcutaneous body fat is a distinct anatomical entity with unique physiology, metabolism, and disease. The main roles of fat are energy storage, hormone production, and insulation. Free fatty acids and glycerol can be assembled into triglycerides, and conversely triglycerides can undergo lipolysis. Insulin is the key hormone that controls this fine balance. Pregnancy is associated with increased truncal body fat and breast ptosis. Cellulite is the padded appearance of fat and there is currently no single proven treatment. Lipomas are the most common soft tissue tumour and can be treated with excision or liposuction. Lipodystrophies are disorders characterized by the selective loss of body fat and lipofilling is a useful treatment modality. Panniculitis is the inflammation of subcutaneous fat and treatment involves dealing with the precipitating cause. The plastic surgeon should be familiar with the anatomy, physiology, and pathology of fat in order to treat this wide array of adipose-related conditions.

The Rhino-Lip-Lifting: A Novel Proposal for Midface Profileplasty Performed as a Single Surgical Procedure

As facial aging occurs, aesthetic changes of the nasal tip and the senile upper lip include tip ptosis, increase in skin length, loss of vermillion height and Cupid's bow, and vertical wrinkles.Regardless of the rejuvenation procedure, the lips are not to be considered as a single anatomical entity, instead they should be placed in a strong correlation with the nose. The aim of this study is therefore to demonstrate the effectiveness of the association of primary closed rhinoplasty together with indirect subnasal lip-lifting technique.A total of 45 patients were enrolled in this study and underwent primary closed rhinoplasty with indirect subnasal lip-lifting. The result showed an overall average nasolabial angle width reduction of 10.9% and lip length shortening of 23.5%, as assessed 1 year after the surgical procedure. When comparing before and after photographs and using the Subjective Global Aesthetic Improvement Scale (sGAIS), the overall satisfaction was rated 4.4 on 5, as “much improved.”The authors show that lips should not be considered as a single anatomical entity but that they indeed must be placed in a strong correlation with the nose since a droopy nasal tip is likely to be considered unattractive and is often associated with the appearance of a long nose with covered philtrum and upper lip.Tip rotation to a normal nasolabial angle width could grant a beneficial impact on overall nose aesthetics; however, it may not fully balance the midface profile as it is the “lip position-to-incisal show” ratio that defines beauty, whereas the balance among forehead, nasal tip, upper lip, and chin is what defines the contour of harmonious profiles.This is the first patient series to show that the combination of closed rhinoplasty and indirect lip-lifting is a highly effective, safe, and reliable procedure to address profile rejuvenation.

Gene Expression-Related Changes in Morphologies of Organelles and Cellular Component Organization in Mucopolysaccharidoses

Mucopolysaccharidoses (MPS) are inherited metabolic diseases characterized by accumulation of incompletely degraded glycosaminoglycans (GAGs) in lysosomes. Although primary causes of these diseases are mutations in genes coding for enzymes involved in lysosomal GAG degradation, it was demonstrated that storage of these complex carbohydrates provokes a cascade of secondary and tertiary changes affecting cellular functions. Potentially, this might lead to appearance of cellular disorders which could not be corrected even if the primary cause of the disease is removed. In this work, we studied changes in cellular organelles in MPS fibroblasts relative to control cells. All 11 types and subtypes of MPS were included into this study to obtain a complex picture of changes in organelles in this group of diseases. Two experimental approaches were employed, transcriptomic analyses and electron microscopic assessment of morphology of organelles. We analyzed levels of transcripts of genes grouped into two terms included into the QuickGO database, ‘Cellular component organization’ (GO:0016043) and ‘Cellular anatomical entity’ (GO:0110165), to find that number of transcripts with significantly changed levels in MPS fibroblasts vs. controls ranged from 109 to 322 (depending on MPS type) in GO:0016043, and from 70 to 208 in GO:0110165. This dysregulation of expression of genes crucial for proper structures and functions of various organelles was accompanied by severe changes in morphologies of lysosomes, nuclei, mitochondria, Golgi apparatus, and endoplasmic reticulum. Interestingly, some observed changes occurred in all/most MPS types while others were specific to particular disease types/subtypes. We suggest that severe changes in organelles in MPS cells might arise from dysregulation of expression of a battery of genes involved in organelles’ structures and functions. Intriguingly, normalization of GAG levels by using recombinant human enzymes specific to different MPS types corrected morphologies of some, but not all, organelles, while it failed to improve regulation of expression of selected genes. These results might suggest reasons for inability of enzyme replacement therapy to correct all MPS symptoms, particularly if initiated at advanced stages of the disease.

Anatomical Understanding of Vitapa Marma by Means of Cadaveric Dissection

Background of study: Marma are those spots on the body which are painful on application of pressure and shows abnormal pulsation. Marma are conglomeration of Mamsa, Sira, Snayu, Asthi and Sandhi. [1] There are 107 Marma.[2] Individual Marma are catagorized on different groups on the basis of various aspects such as Rachananusara, Parinamanusara, Sthananusara, Pramananusara etc. Vitapa Marma is the Adhoshakagata, Vaikalyakara Marma[3,4] of 1 Anguli Pramana.[5,6] The explanation of the Vitapa Marma in the classical texts cannot specify the particular anatomical entity so the study is undertaken to fulfill the deficit knowledge. Objectives: To evaluate the Snayu & Sira component of Vitapa Marma. To evaluate structural entity of Vitapa Marma in relation to given Pramana and Viddha Lakshana. Materials and methods: Cadaveric Study. Observation of the structures exposed during cadaveric dissection and correlation with textual description were done and the conclusion was drawn. Results: Vitapa Marma lies on the inguinal region at the superficial inguinal ring. The anatomical components of this Marma are the structures related to and passing through superficial inguinal ring.

AN OBSERVATIONAL CROSS-SECTIONAL STUDY TO EXPLORE THE VAIKALYAKARA EFFECT OF JANU MARMA THROUGH ANATOMICAL CONSIDERATIONS OF KNEE JOINT INJURIES RELATED TO SPORTS

Introduction: The aim of the study was to determine the time to and rate of the return to sports (RTS) after knee joint injury. The data obtained was analysed to fine line the commonly affected anatomical entity re-sponsible and to correlate with Janumarma. Methodology: The symptoms assessed from the case profor-ma and MRI were compared to the Janu Marmabhighatha Lakshanas. The grading of injury and Khan-jatha was determined with the help of unique scoring adapted for the study. Results: Based on literary re-view Khanjatha can be defined as a weakness in limbs resisting its complete functional mobility. Anterior Cruciate Ligament (ACL) was found to be the most vulnerable structure. Cadaveric dissection studies showed the morphological significance of ACL to be placed as a Kandara has been surmised. Conclusion: Khanjatha is concluded as the difficulty in regaining complete functional mobility, which was observed in 70% of ACL injuries highlighting the plausible correlation of ACL as Janumarma.

AN OBSERVATIONAL CROSS-SECTIONAL STUDY TO EXPLORE THE VAIKALYAKARA EFFECT OF JANU MARMA THROUGH ANATOMICAL CONSIDERATIONS OF KNEE JOINT INJURIES RELATED TO SPORTS

Introduction: The aim of the study was to determine the time to and rate of the return to sports (RTS) after knee joint injury. The data obtained was analysed to fine line the commonly affected anatomical entity re-sponsible and to correlate with Janumarma. Methodology: The symptoms assessed from the case profor-ma and MRI were compared to the Janu Marmabhighatha Lakshanas. The grading of injury and Khan-jatha was determined with the help of unique scoring adapted for the study. Results: Based on literary re-view Khanjatha can be defined as a weakness in limbs resisting its complete functional mobility. Anterior Cruciate Ligament (ACL) was found to be the most vulnerable structure. Cadaveric dissection studies showed the morphological significance of ACL to be placed as a Kandara has been surmised. Conclusion: Khanjatha is concluded as the difficulty in regaining complete functional mobility, which was observed in 70% of ACL injuries highlighting the plausible correlation of ACL as Janumarma.