Selective and predicable amine conjugation sites by kinetic characterization under excess reagents

The site selectivity for lysine conjugation on a native protein is difficult to control and characterize. Here, we applied mass spectrometry to examine the conjugation kinetics of Trastuzumab-IgG (Her-IgG) and α-lactalbumin under excess linker concentration ([L]0) based on the modified Michaelis–Menten equation, in which the initial rate constant per amine (kNH2 = Vmax/NH2/KM) was determined by the maximum reaction rate (Vmax/NH2) under saturated accessible sites and initial amine–linker affinity (1/KM). Reductive amination (RA) displayed 3–4 times greater Vmax/NH2 and a different panel of conjugation sites than that observed for N-hydroxysuccinimide ester (NHS) chemistry using the same length of polyethylene glycol (PEG) linkers. Moreover, faster conversion power rendered RA site selectivity among accessible amine groups and a greater tunable range of linker/protein ratio for aldehyde-linkers compared to those of the same length of NHS-linkers. Single conjugation with high yield or poly-conjugations with site homogeneity was demonstrated by controlling [L]0 or gradual addition to minimize the [L]0/KM ratio. Formaldehyde, the shortest aldehyde-linker with the greatest 1/KM, exhibited the highest selectivity and was shown to be a suitable probe to predict conjugation profile of aldehyde-linkers. Four linkers on the few probe-predicted hot spots were elucidated by kinetically controlled RA with conserved drug efficacy when conjugated with the payload. This study provides insights into controlling factors for homogenous and predictable amine bioconjugation.

Large Tunable 16-Tupled Millimeter Wave Generation Utilizing Optical Carrier Suppression With a Tunable Sideband Suppression Ratio

Coping up with the rising bandwidth demands for 5G ultra-high speed applications, utilizing millimeter (MM) wave spectrum for data transmission over the radio over a fiber-based system is the ideal approach. In this study, a highly conversant and spectrally pure photonic generation of a 16-tupled MM wave signal using a series-connected DD-MZM with a lower modulation index, a splitting ratio, and a wider tunable range is presented. A 160-GHz MM wave is generated through a double sideband optical carrier suppression technique having an optical sideband suppression ratio (OSSR) of 69 dB and a radio frequency sideband suppression ratio (RSSR) of 40 dB. However, the OSSR and the RSSR are tunable with values greater than 15 dB when the modulation index (M.I.) varies from 2.778 to 2.873, ±8° phase drift, and a 15-dB enhancement in the OSSR with a wider nonideal parameter variation range giving acceptable performance can be seen in the model as compared with previous research works.

Photonic microwave and RF programmable true time delays for phased array antennas using a soliton crystal Kerr micro-comb source

We demonstrate significantly improved performance of a microwave true time delay line based on an integrated optical frequency comb source. The broadband micro-comb (over 100 nm wide) features a record low free spectral range of 49GHz, resulting in an unprecedented record high channel number (81 over the C band) – the highest number of channels for an integrated comb source used for microwave signal processing. We theoretically analyze the performance of a phased array antenna and show that this large channel count results in a high angular resolution and wide beam steering tunable range. This demonstrates the feasibility of our approach as a competitive solution towards implementing integrated photonic true time delays in radar and communications systems.

Photonic crystal nanobeam cavities with lateral fins

We present the design, fabrication, and characterization of suspended arrays of small volume, high quality factor (Q) silicon nitride photonic crystal nanobeam (PCNB) cavities with lateral nanorod fin structures. By controlling the alignment position of the fins with respect to the air holes, the resonance wavelength and Q-factor of the PCNB cavities can be tuned to realize the desired performance. Measured tunable range of 2 × 104 and 10 nm is achieved for Q-factor and resonance wavelength, respectively, with the highest Q-factor measured at 2.5 × 104. Incorporating such nanorod fins into the nanobeam cavity is demonstrated to provide improved mechanical support, thermal transport, and channels of lateral carrier injection for the suspended PCNB. The proposed PCNB cavities with lateral fins are advantageous for energy efficient, ultra-compact lasers, modulators, filters, and sensors.

Wide Programmable Range Fourth-Order, Fully-Differential Sallen-Key MOSFET-C LPF for Impedance Spectroscopy Measurements and Self-X Sensory Electronics in Industry 4.0

This paper picks up the need for a wide range programmable corner frequency for anti-aliasing and antiimaging filters in on-chip impedance spectroscopy and sensor signal readout circuitry with self-X properties (selfdiagnosing/healing) for industry 4.0 applications. A fourthorder wide tunable range MOSFET-C low pass filter is designed by using XFAB 0.35 μm CMOS technology and Cadence design tools. The proposed circuit is based on fully differential Sallen-Key architecture with Butterworth approximation. It covers a frequency range from 30 Hz up to 7 MHz. Tunability is achieved using a potentially high resistance and linearized configurable MOS resistor to control the filter pole frequency. The configurable elements in the circuit serve as tuning knobs to be controlled by machine learning. The physical design area is 0.39mm2.

A novel 4-DOF wide-range tunable frequency selective surface using an origami “eggbox” structure

Shape-changing mechanical metamaterials have drawn the attention of researchers toward the development of continuous-range tunable frequency selective surfaces (FSSs). In this paper, a novel tunable FSS utilizing an origami-inspired “eggbox” structure is presented featuring four-degrees of freedom that can change the frequency response of two orthogonal linear polarizations. The centrosymmetric “eggbox” structure can be folded or rotated along two axes that lead to unprecedented reconfigurability compared to traditional Miura-Ori-based structures which have fewer degrees of control. The utilized cross-shaped dipole FSS element shows enhanced bandwidth, support for orthogonal linear polarization, and ease of fabrication. The prototype is fabricated using a low-cost fully additive inkjet printing process with silver nanoparticle conductive ink. The outcome of this study shows a 25% frequency tunable range over two polarization directions. The design can be an ideal spatial filtering candidate for advanced ultra-wideband terrestrial and space applications.

Photocontrollable Resistivity Change in Nanoparticle-Doped Liquid Crystal Alignment Layer: Voltage Holding and Discharging Properties of Fringe-Field Switching Liquid Crystal Modes

In liquid crystal (LC) displays, deriving an optimum resistance level of an LC alignment polyimide (PI) layer is important because of the trade-off between the voltage holding and surface-discharging properties. In particular, to apply a power-saving low-frequency operation scheme to fringe-field switching (FFS) LC modes with negative dielectric LC (n-LC), delicate material engineering is required to avoid surface-charge-dependent image flickering and sticking problems, which severely degrade with lowering operation frequency. Therefore, this paper proposes a photocontrolled variable-resistivity PI layer in order to systematically investigate the voltage holding and discharging properties of the FFS n-LC modes, according to the PI resistivity (ρ) levels. By doping fullerene into the high-ρ PI as the photoexcited charge-generating nanoparticles, the ρ levels of the PI were continuously controllable with a wide tunable range (0.95 × 1015 Ω∙cm to 5.36 × 1013 Ω∙cm) through Ar laser irradiation under the same LC and LC alignment conditions. The frequency-dependent voltage holding and discharge behaviors were analyzed with photocontrolled ρ variation. Thus, the proposed experimental scheme is a feasible approach in PI engineering for a power-saving low-frequency FFS n-LC mode without the image flickering and image sticking issues.

The Design and Manufacturing Process of an Electrolyte-Free Liquid Metal Frequency-Reconfigurable Antenna

This communication provides an integrated process route of smelting gallium-based liquid metal (GBLM) in a high vacuum, and injecting GBLM into the antenna channel in high-pressure protective gas, which avoids the oxidation of GBLM during smelting and filling. Then, a frequency-reconfigurable antenna, utilizing the thermal expansion characteristic of GBLM, is proposed. To drive GBLM into an air-proof space, the thermal expansion characteristics of GBLM are required. The dimensions of the radiating element of the liquid metal antenna can be adjusted at different temperatures, resulting in the reconfigurability of the operating frequency. To validate the proposed concept, an L-band antenna prototype was fabricated and measured. Experimental results demonstrate that the GBLM in the antenna was well filled, and the GBLM was not oxidized. Due to the GBLM being in an air-proof channel, the designed liquid metal antenna without electrolytes could be used in an air environment for a long time. The antenna is able to achieve an effective bandwidth of over 1.25–2.00 GHz between 25 °C and 100 °C. The maximum radiation efficiency and gain in the tunable range are 94% and 2.9 dBi, respectively. The designed antenna also provides a new approach to the fabrication of a temperature sensor that detects temperature in some situations that are challenging for conventional temperature sensing technology.