Findings of qualitative studies on Understanding by Design: A meta-synthesis

Designed by the meta-synthesis method, the researchers examined the studies designed on the Understanding by Design (UbD) and implemented and their findings were qualitatively evaluated. It tried to obtain a comprehensive and holistic perspective on the effects and reflections of the model. 48 UbD studies were accessed from 6 databases, and 12 research findings were included after three criteria in this meta-synthesis. The findings were reached through a six-stage data analysis process; they were analyzed inductively with content analysis. The validity and credibility of this analysis process have been brought under control with the coding reliability processes and the audit technique. According to the conclusions, in the development of units based on UbD, teachers generally did not create goals/standards by collecting evaluation evidence of UbD; it was concluded that the inadequacy of this was mostly due to model inexperience, the school's facilities/conditions, teacher stagnation and emotional state, and inadequate pedagogy knowledge. Findings showed that teaching based on UbD improves students' cognitive development and participatory insights. This meta-synthesis study guides researchers who want to reference UbD as a design framework in a unit study in the overall assessment of UbD.

Investigating the Teaching Practice Framework in the Departments of Primary Education in Greece: A Sociological Approach

The aim of this research is to analyze and compare the Teaching Practice (TP) in the Departments of Primary Education (DPE) in Greece, in terms of objectives, structure and evaluation. The data for this research was drawn from the Study Guides of nine Departments that we examined in Greece, and they were analyzed using the technique of document analysis. The analysis utilized B. Bernstein’s theoretical framework. The analysis of the data revealed that the TP framework is differentiated across the Departments in terms of time demarcation and student evaluation. These differentiations illustrate the differences in the Departments’ philosophy and organization. Nevertheless, the chief aim of all the Departments is to familiarize future teachers with their duties and responsibilities, an aim which is achieved through the gradual introduction of the trainees into the school field.

Towards Explaining Knowledge Hiding through Relationship Conflict, Frustration, and Irritability: The Case of Public Sector Teaching Hospitals

Organizations grow and excel with knowledge sharing; on the other hand, knowledge hiding is a negative behavior that impedes innovation, growth, problem solving, and timely correct decision making in organizations. It becomes more critical in the case of teaching hospitals, where, besides patient care, medical students are taught and trained. We assume that negative emotions lead employees to hide explicit knowledge, and in the same vein, this study has attempted to explain the hiding of explicit knowledge in the presence of relational conflicts, frustration, and irritability. We collected data from 290 employees of a public sector healthcare organization on adopted scales to test conjectured relationships among selected variables. Statistical treatments were applied to determine the quality of the data and inferential statistics were used to test hypotheses. The findings reveal that relationship conflicts positively affect knowledge hiding, and frustration partially mediates the relationship between relationship conflicts and knowledge hiding. Irritability moderates the relationship between relationship conflicts and frustration. The findings have both theoretical and empirical implications. Theoretically, the study tests a novel combination of variables, and adds details regarding the intensity of their relationships to the existing body of literature. Practically, the study guides hospital administrators in managing knowledge hiding, and informs on how to maintain it at the lowest possible level.

CNTM-02. Regulation of glioma-network integration by tumor mediated secretion of TSP-1

Glioma exists in the complex neural circuitry of the brain, making the interface between neoplastic and healthy neurons and glia potentially damaging to long range neural networks and stimulatory to tumor growth. Thrombospondin-1 (TSP-1), an astrocyte derived neurogenic factor expressed by glia of the normal brain, has been found to be upregulated in intratumoral regions with high network functional connectivity (HFC). This modified cell signaling represents cancer cell hijacking of normal physiology with direct impact on tumor biology. There is emerging evidence that neuronal activity influences glioma proliferation and gliomas promote neuronal hyperexcitability. In humans, we have recently shown that bidirectional cellular interactions between gliomas and neurons alter cognitive circuit dynamics and ultimately patient survival. Previously, a subpopulation of human high-grade glioma cells which are enriched for tumor cells with synaptogenic potential were identified (HFC-IHDwtGBM). We plan to study the mechanisms of TSP1 signaling in three different established glioma models (1) HFC-IDHwtGBM hippocampal neuron co-culture, (2) HFC-IDHwtGBM + induced neuron organoids, (3) patient derived xenografts (PDX) for in vivo GCaMP calcium imaging. This project aims to test the hypothesis that increased TSP-1 secretion from HFC-IHDwtGBM cells plays a central role in the maintenance of an invasive and proliferative tumor phenotype when compared with LFC-IHDwtGBM PDX. We hope our study guides future work focused on preventing the infiltration of tumor cells into healthy brain tissues.

A Food Safety Education Needs Assessment for Small-Scale Produce Growers Interested in Value-Added Food Production

Production of value-added foods is of growing interest to small-scale produce growers. However, previous studies reported that they lack sufficient food safety awareness and have limited knowledge of applicable food safety regulations. Targeted food safety education can help to address these challenges. This study used focus groups and a combination of web-based and printed surveys to evaluate growers’ attitudes toward and knowledge of value-added business and to assess their food safety education needs. A total of 136 Indiana produce growers participated in the study, including 38 focus group participants and 98 survey participants. Overall, growers were aware of the importance of food safety, with 78% of the survey participants agreeing strongly that food safety was a top priority in value-added businesses. Most survey participants (82%) self-reported being knowledgeable about food safety. Most were confident in their ability to run a successful value-added business. The top two barriers that hindered growers from learning about food safety were time limitations and an overwhelming amount of information. Growers preferred to receive additional educational materials on selling non-home-based vendor products, communication strategies with regulators and inspectors, and marketing strategies; they trusted the information from extension educators and university extension publications the most. This study guides the development of future food safety education programs for small-scale growers who are interested in establishing a value-added business.

Method for Directing Collaborative Study Guide Construction by Undergraduate Student Groups

Students often request study guides; however, the impact of study guides on student learning is mixed. Here, some evidence on student study guide usage and collaborative learning is briefly reviewed. This information helped to shape the development of a collaborative activity where student groups create their own study guide questions based on the chapter learning outcomes. Requiring students to collaborate and create their own study guides may encourage a higher engagement with and deeper processing of the course content.

Modes of Study Handbook for Students and Educators

The aim of this handbook is to support the further development of multilingual and multimodal approaches to academic study at JYU. This handbook has been developed within the Faculty of Education and Psychology as part of the Creating Space pedagogical development project during the 2019-2020 academic year, drawing on and developing existing study guides by JYU . We hope, however, that this handbook is useful beyond our Faculty and that it adds language and cultural considerations to the existing study guides. We have provided brief explanations of different types of assignments and academic activities that are part of academic study and relevant to teaching in higher education. We have also included ‘good to know’ pointers for students and teachers, as well as suggestions for how different languages can be used as resources in academic study, and highlighting ways in which cultural awareness can support academic participation. The call-out bubbles are quotations from students and teachers who have participated in the Creating Space events. We hope that this handbook helps to raise awareness regarding the value and role of different languages, cultures and modalities in academic study and provides useful pedagogical tools for students and teachers.

Methodological guidelines for isolation and purification of plant extracellular vesicles

ABSTRACTPlant extracellular vesicles (EVs) have become the focus of rising interest due to their important roles in the cross-kingdom trafficking of molecules from hosts to interacting microbes to modulate pathogen virulence. However, the isolation of pure intact EVs from plants still represents a considerable challenge. Currently, plant EVs have been isolated from apoplastic washing fluid (AWF) using a variety of methods. Here, we compare two published methods used for isolating plant EVs, and provide a detailed recommended method for AWF collection from Arabidopsis thaliana, followed by EV isolation via differential ultracentrifugation. To further separate and purify specific subclasses of EV from heterogeneous vesicles, sucrose or iodixanol density-based separation and immunoaffinity capture are then utilized. We found that immunoaffinity capture provides a significant advantage for specific EV isolation when suitable specific EV biomarkers and their corresponding antibodies are available. Overall, this study guides the selection and optimization of EV isolation methods for desired downstream applications.

Effects of Rhizosphere Microbial Composition on the Growth of Cajanus Cajan in Coal Gangue Reclaimed Soil

Planting the economically sustainable Cajanus cajan crop in coal gangue dump as an approach for ecological management has attracted extensive attention from scholars worldwide. Microorganisms play important roles in ecological restoration. However, there have been few reports about the microbial composition and functions of Cajanus cajan root systems planted in plateau waste dumps. In this study, the gangue dump (1,390 m above sea level) in Guizhou Plateau was covered with soil and planted with YN and JX Cajanus cajans. After one year, the Cajanus cajan root system was collected, and high-throughput sequencing was utilized to examine the bacterial and fungal biodiversity. The bacteria in all samples were assigned to six phyla and 60 genera. The unclassified_f_Enterobacteriaceae and Cedecea were the most abundant genera. The fungi were assigned to six phyla and 143 genera and Fusarium was the most abundant genus. Venn and heatmap analyses revealed differences in the dominant root species among Cajanus cajan varieties, Lactococcus and Fusarium. In JX and YN, Pseudoarthrobacter is a unique genus of bacteria; in JX, Robillarda is a unique genus of fungi; and in YN, Scytalidium is a unique genus of fungi. The endophytic fungi mainly play the roles of saprotroph, plant pathogen, and endophyte. Among the bacterial properties, L-arabinose isomerase and adenosine triphosphatase were the most abundant in all samples, while chitinase, catalase, and laccase played important roles in photosynthesis, degradation of lignin, chitin, and chitosan. This study guides the selection of reclamation plants and strategies for the migration and restriction of heavy metals in soil.