Modeling Basin-Scale Impacts of Cultivation Practices on Cotton Yield and Water Conservation under Various Hydroclimatic Regimes

The SWAT model equipped with an improved auto-irrigation function was used to assess the impacts of cultivation practices on irrigated and dryland cotton yield and water conservation in the Texas Panhandle. Results showed the largest irrigation depth led to reductions in irrigation and crop evapotranspiration (ETc) with slightly increased cotton yields compared to the baseline scenarios under different hydroclimatic regimes. However, soil water content and surface runoff values were increased when using the largest irrigation depth. The opposite results were observed for the small irrigation depth. Early planting of cotton resulted in decreased irrigation and ETc, and increased cotton yields under both irrigated and dryland conditions, particularly in normal and wet years. By contrast, the late planting scenarios indicated the opposite for those variables. Simulated hydrologic variables were relatively stable using various maturity cultivars. Nevertheless, greater than 10% reductions in irrigated cotton yield under diverse hydroclimatic years and dryland yields during normal and wet years were identified in the long-season cotton. The opposite was determined for the short-season cotton. These outcomes suggest that a larger irrigation depth, earlier planting date, and short-season cultivar are promising cultivation practices for improving cotton yield and water conservation in the Texas Panhandle.

How Archaeologists Can Effectively and Ethically Cultivate Responsible and Responsive Stewards

The objective of this article is to share how professional archaeologists can leverage their knowledge and influence to develop collectors and amateur archaeologists into responsible and responsive stewards (RRS). This approach furthers the primary purposes of the National Historic Preservation Act as well as the ethics of the Society for American Archaeology. I share my own journey about finding artifacts in the Texas Panhandle, yearning to understand more about them, and how a poster in a gas station led me to visiting a local Stone Age Fair where I met a professional archaeologist. This archaeologist mentor was willing to answer my questions and guide me to greater understanding of archaeological ethics and practice, which prompted me to document everything I had found. In turn, I have shared my experience with many other nonprofessionals. Archaeologist mentors can have great influence if they are willing to meet collectors and amateur archaeologists on their level. Ultimately, collectors and archaeologists share a love of artifacts and a passion for the history behind them.

PSII-B-22 High-impact minimester course in animal science

Many Animal Science students have little exposure to working livestock production systems prior to entering college. As such, they can lack insight into day-to-day challenges and rationale behind decision making in these systems, opening the door for adoption of misconceptions frequently promoted in popular press. Additionally, students identify lack of first-hand knowledge and experience in the industry as a challenge to their educational success. Field trips stimulate interest and motivation, provide context for learning, and influence long-term career goals, but are underutilized in higher education. Potential impact of such experiences prompted creation of the Texas Panhandle Beef Production Tour, a 2-credit hour course that takes place during the spring minimester. To cement learning through reflection, students were asked to respond to a series of questions before, during, and after visiting beef production sites in the Texas Panhandle to probe preconceptions, observations, and outcomes of the experience. We then performed a retroactive evaluation of these reflections (n = 22) to determine cogent themes. Emergent themes included surprise at the intensive systems of data collection and management and the level of technology used at each site. Cattle were calmer and more comfortable than expected at feedlots, dairy, and packing plants visited. Students expressed new appreciation and understanding of course material and a desire to share their insights with others after completing the tour. Finally, participants gained a broader view of industry opportunities and returned with renewed motivation to pursue additional hands-on opportunities. Participation in this course provided valuable insight into the livestock production industry and motivated students to explore new career options and address their own preconceptions of the industry through independent inquiry. Creation of similar courses may be useful to address misconceptions, create personal connections with course material, and broaden career interests in undergraduate animal science students.

Two New Bacterial Pathogens of Peanut Causing Early Seedling Decline Disease Identified in the Texas Panhandle

Peanut (Arachis hypogaea L.) is cultivated in tropical and subtropical regions of the world as an important source of oil and protein. Until now, bacterial wilt, caused by Ralstonia solanacearum, was the only known bacterial disease of peanut. In 2020, widespread incidence of poor stand establishment were observed in multiple production fields planted to the Spanish-type peanut varieties in the Texas Panhandle. The observed symptoms included seed rot, pre- and post-emergence damping-off, poor seedling vigor and death, and poorly developed root systems with little or no nodule formation. Subsequent diagnosis of symptomatic seedlings recovered two bacterial species identified by BLAST using 676 bp and 661 bp 16S rRNA fragments as a R. species and a Pantoea sp., respectively. To investigate a possible causative role of these bacteria in the observed peanut disease, the pathogenicity of the two isolates was evaluated under greenhouse conditions relying on Koch’s postulates. Cell suspensions of the two bacteria, separately and in combination, were used to inoculate seeds of a Valencia-type peanut variety with no history of the disease and found to be pathogenic on the resultant seedling plants. Symptoms that developed on the inoculated plants were similar to the symptoms initially observed in the field, including seed rot, pre- and post-emergence damping off, poor seedling vigor and root establishment. The two bacteria were also successfully recovered from inoculated and symptomatic plants, thus satisfying Koch’s postulates. Given the early onset of symptom development on affected seeds and seedlings, a seedborne origin of the disease, described here as early-decline bacterial disease of peanut, was investigated in the same batches of peanut seeds that were planted, as well as seeds later harvested in some of the affected fields. Identical bacterial species, on the basis of 16S rRNA identity, were recovered from all of the seeds evaluated indicating that the bacteria are both seedborne and seed-transmissible. Multi-locus sequence analysis (MLSA) involving six genes (dnaK, fumC, gyrB, murG, trpB, and tuf) showed that these new strains are most closely related to R. pickettii and P. dispersa, but also phylogenetically distinct. The two bacteria were designated Ralstonia sp. strain B265 and Pantoea sp. strain B270. Losses from the disease in affected fields in 2020 averaged fifty percent ($1.12 million) from a total of nine production fields. Findings from this study provide evidence for two new bacterial pathogens of peanuts capable of infecting Spanish and Valencia peanut varieties.

Laboratory and field studies of ice-nucleating particles from open-lot livestock facilities in Texas

In this work, an abundance of ice-nucleating particles (INPs) from livestock facilities was studied through laboratory measurements from cloud-simulation chamber experiments and field investigation in the Texas Panhandle. Surface materials from two livestock facilities, one in the Texas Panhandle and another from McGregor, Texas, were selected as dust proxies for laboratory analyses. These two samples possessed different chemical and biological properties. A combination of aerosol interaction and dynamics in the atmosphere (AIDA) measurements and offline ice spectrometry was used to assess the immersion freezing mode ice nucleation ability and efficiency of these proxy samples at temperatures above −29 ∘C. A dynamic filter processing chamber was also used to complement the freezing efficiencies of submicron and supermicron particles collected from the AIDA chamber. For the field survey, periodic ambient particle sampling took place at four commercial livestock facilities from July 2017 to July 2019. INP concentrations of collected particles were measured using an offline freezing test system, and the data were acquired for temperatures between −5 and −25 ∘C. Our AIDA laboratory results showed that the freezing spectra of two livestock dust proxies exhibited higher freezing efficiency than previously studied soil dust samples at temperatures below −25 ∘C. Despite their differences in composition, the freezing efficiencies of both proxy livestock dust samples were comparable to each other. Our dynamic filter processing chamber results showed on average approximately 50 % supermicron size dominance in the INPs of both dust proxies. Thus, our laboratory findings suggest the importance of particle size in immersion freezing for these samples and that the size might be a more important factor for immersion freezing of livestock dust than the composition. From a 3-year field survey, we measured a high concentration of ambient INPs of 1171.6 ± 691.6 L−1 (average ± standard error) at −25 ∘C for aerosol particles collected at the downwind edges of livestock facilities. An obvious seasonal variation in INP concentration, peaking in summer, was observed, with the maximum at the same temperature exceeding 10 000 L−1 on 23 July 2018. The observed high INP concentrations suggest that a livestock facility is a substantial source of INPs. The INP concentration values from our field survey showed a strong correlation with measured particulate matter mass concentration, which supports the importance of size in ice nucleation of particles from livestock facilities.

Ice-nucleating particles in precipitation samples from the Texas Panhandle

Ice-nucleating particles (INPs) influence the formation of ice crystals in clouds and many types of precipitation. This study reports unique properties of INPs collected from 42 precipitation samples in the Texas Panhandle region from June 2018 to July 2019. We used a cold stage instrument called the West Texas Cryogenic Refrigerator Applied to Freezing Test system to estimate INP concentrations per unit volume of air (nINP) through immersion freezing in our precipitation samples with our detection capability of > 0.006 INP L−1. A disdrometer was used for two purposes: (1) to characterize the ground-level precipitation type and (2) to measure the precipitation intensity as well as size of precipitating particles at the ground level during each precipitation event. While no clear seasonal variations of nINP values were apparent, the analysis of yearlong ground-level precipitation observation as well as INPs in the precipitation samples showed some INP variations, e.g., the highest and lowest nINP values at −25 ∘C both in the summer for hail-involved severe thunderstorm samples (3.0 to 1130 INP L−1), followed by the second lowest at the same temperature from one of our snow samples collected during the winter (3.2 INP L−1). Furthermore, we conducted bacteria community analyses using a subset of our precipitation samples to examine the presence of known biological INPs. In parallel, we also performed metagenomics characterization of the bacterial microbiome in suspended ambient dust samples collected at commercial open-lot livestock facilities (cattle feedyards hereafter) in the Texas Panhandle (i.e., the northernmost counties of Texas, also known as “West Texas”) to ascertain whether local cattle feedyards can act as a source of bioaerosol particles and/or INPs found in the precipitation samples. Some key bacterial phyla present in cattle feedyard samples appeared in precipitation samples. However, no known ice nucleation active species were detected in our samples. Overall, our results showed that cumulative nINP in our precipitation samples below −20 ∘C could be high in the samples collected while observing > 10 mm h−1 precipitation with notably large hydrometeor sizes and an implication of cattle feedyard bacteria inclusion.

Characterization of Physicochemical Properties of Feedlot Dust Ice Crystal Residuals (ICRs)

This study considers how feedlot dust size and composition contribute to atmosphericice nucleation and the formation of local cloud and precipitation in the Texas Panhandle. [...]