Comparison of ten biochemical laboratory tests before and after treatment by statins

Results of 10 biochemical tests of 172 patient data (among them 84 men data and 88 women data, resp.) before and after administration of statins were thoroughly studied. All monitored patients are characterized by disorders of lipoprotein metabolism or other kind of dislipidaemia. The calculations were performed using four chemometrical methods facilitating quantification and visualization of the statin effect upon most important biochemical parameters, mainly lipid markers, and allowing classification of the patient blood samples taking into account whether the patient has been or has not been medicated by a statin drug.

Patient with False Negative RT-PCR for COVID-19 referred to Cancer Hospital for Lung Cancer Screening

Background: To describe the characteristics and outcomes of patients with a clinical diagnosis of COVID-19 and false-negative SARS-CoV-2 reverse transcription-PCR (RT-PCR), and develop and internally validate a diagnostic risk score to predict risk of COVID-19 (including RT-PCR-negative COVID-19) among medical treatment. Case presentation: Herein, we report a 42-year-old male patient from myagdi district Nepal presenting with high grade fever, dry cough, headache and dizziness on the August 10, 2021 during second phase of COVID-19 pandemic. There is no history of hypertension and diabetes. He went for RT-PCR test at local COVID-19 screening center and reported as negative for RT-PCR test. After that he referred to Lumbini Zonal hospital for further evaluation. RT-PCT swab test was performed again and reported negative. On the chest X-Ray, there was opacity on both lungs and the patient was referred to cancer hospital for lung cancer screening. The patient underwent for HRCT chest and biochemical laboratory tests for further evaluation. The chest High Resolution Computed Tomography (HRCT) indicated ground grass opacity (GGO) with crazy paving a typical COVID-19 interstitial pneumonia. In the biochemical laboratory test, there were elevation in Leukocyte (Total WBC count), Neutrophil, Glucose, Bilirubin Direct, Bilirubin Total, SGOT/AST, SGPT/ALT and Lactate Dehydrogenase (LDH). There was low count found in Lymphocyte, Eosinophil and Monocyte. These laboratory parameters findings are typical sign of COVID-19 patients. Then patient was isolated and treatment of given according to COVID-19 treatment guidelines. On September 12, 2021, all diagnostic tests showed that patient recovered from COVID-19. Conclusion: It is safe to suggest that a symptomatic patient with typical chest HRCT and lab findings for COVID-19 should be quarantined or isolated even with 2 negative RT-PCR tests.

Biological markers as predictors of postoperative neurocognitive disorders

Introduction. The literature review presents new definitions of the concepts of postoperative cognitive dysfunctions, taking into account the new nomenclature according to DSM-5 (Diagnostic and Statistical Manual of mental disorders, fifth edition — Diagnostic and statistical manual of mental disorders, 5th edition) and time of their manifestations, depending on the age of the surgical intervention performed. The main discussed tactical aspects of the anesthesiologist's management of patients at risk of cognitive impairment in the postoperative period are outlined. The main biological markers, predictors of the development of cognitive postoperative disorders, are emphasized.Aim of the study: to highlight the main discussed tactical points of anesthesiologist's management of patients, to outline more promising aspects of biochemical laboratory diagnosis of postoperative neurocognitive disorders.Materials and methods. The data were analyzed and systematized based on scientific publications in the general PubMed database for the period 2016-2020.Results and discussion. New research advances in the search for biological targets, markers and predictors of postoperative neurocognitive disorders at the molecular level are presented, allowing a more precise impact on the pathogenetic mechanisms of neuronal inflammation and assessment of the potential damage to the cognitive status of the patient. The paper outlines more promising aspects of biochemical laboratory diagnosis using markers of innate immunity response to surgical trauma and the resulting systemic inflammation. Conclusion. Problems requiring further study to find pathogenetic therapy for postoperative neurocognitive disorders have been identified.

Efficacy and safety of different terbinafine regimens in patients of recurrent tinea corporis and cruris

Background: Tinea corporis and cruris is said to be recurrent when there is relapse of sign and symptoms after 6 weeks of cure. Recently, there has been increase in cases of recurrent tinea corporis and cruris, with atypical lesions. This study was done to establish efficacy and safety of different terbinafine regimens against recurrent tinea corporis and cruris.Methods: Sixty patients with clinically and mycologically diagnosed recurrent tinea corporis and cruris were enrolled and divided into three groups. Group A was administered oral terbinafine 500 mg once daily for 2 weeks, group B was given terbinafine 250 mg once daily for double duration i.e., 4 weeks, and group C was given standard treatment which is 250 mg once daily for 2 weeks. Physician assessment four-point scale (PA4PS) and KOH wet mount were assessed for clinical and mycological efficacy. Biochemical laboratory parameters (liver function tests and kidney function tests) and adverse drug reactions were assessed for safety.Results: At the end of 6 weeks, reduction in PA4PS from baseline was 46.5%, 95.8%, and 20.4% in groups with double dose, double duration and standard therapy respectively with statistically significant (p<0.05) improvement in group with double duration. Mycological cure at the end of 4 weeks was 80%, 100% and 50%. There was no safety concern in any of the groups.Conclusions: Double duration of terbinafine was found to be more efficacious and safer.

Pelaksanaan Proses Asuhan Gizi Terstandar, Pemberian Diet Tinggi Kalori dan Tinggi Protein terhadap pasien pasca bedah Intususepsi Ileocolic, Post Hemikolektomi Kanan, dan Reseksi Ileum End-to-End Anastomosis : Sebuah Laporan Kasus.

AbstrakPasien pasca bedah memerlukan asuhan gizi khusus untuk percepatan penyembuhan luka, pemulihan, dan mencukupkan asupan gizi, terlebih pada kasus pasien pasca bedah dengan hemikolektomi dan reseksi ileum yang memepengaruhi fungsi saluran cerna, sehingga diperlukan asuhan gizi terstandar dengan pemberian diet tinggi kalori dan tinggi protein (TKTP) agar dapat menurunkan kadar sel darah putih, meningkatkan kadar albumin, serta menormalkan berat badan. Setelah dilakukan intervensi gizi, fisik pasien mulai membaik di tandai dengan sudah bisa berpindah posisi tidur sendiri, hasil laboratorium biokimia RBC, WBC, HGB, HCT, dan albumin menunjukkan penurunan dan peningkatan menuju nilai normal, asupan pasien meningkat dari hari ke hari, namun belum dapat mencapai target (<60%). Pada kasus ini pemulihan kondisi pasien cukup cepat. Fungsi saluran cerna secara bertahap membaik ditandai dengan bentuk makanan pasien yang berubah dari cair hingga bubur kasar. Sehingga intervensi yang diberikan secara keseluruhan tercapai kecuali target asupanAbstractPost-operative patients require special nutritional care to accelerate wound healing, recovery, and adequate nutritional intake. Especially in the case of post-operative patients with hemicolectomy and ileocolic resection that affect the function of the gastrointestinal tract, the nutritional care process is needed by providing a high-calorie and high-protein diet to reduce levels of white blood cells, increase levels of albumin, and to normalize the body weight. After the nutritional intervention, the physical condition began to improve, marked by being able to change his sleeping position, the results of the biochemical laboratory such as RBC, WBC, HGB, HCT, and albumin showed a decrease and increase to normal values. The patient's intake increased from day to day, but can not reach the target yet (<60%). In this case, recovery is quite fast. Gastrointestinal function gradually improves as the patient's diet changes from liquid to coarse porridge, so the overall intervention given is achieved except for the intake target.

Recombinant DNA and Genome-editing Technologies: Embodied Utopias and Heterotopias

Recombinant DNA technology is an essential area of life engineering. The main aim of research in this field is to experimentally explore the possibilities of repairing damaged human DNA, healing or enhancing future human bodies. Based on ethnographic research in a Czech biochemical laboratory, the article explores biotechnological corporealities and their specific ontology through dealings with bio-objects, the bodywork of scientists. Using the complementary concepts of utopia and heterotopia, the text addresses the situation of bodies and bio-objects in a laboratory. Embodied utopias are analyzed as material semiotic phenomena that are embodied by scientists in their visions and emotions and that are related to potential bodies and to future, not-yet-actualized embodiments. As a counterpart to this, the text explores embodied heterotopias, which are always the other spaces, like biotechnological bio-objects that are simulated in computers or stored in special solutions.

Implementasi Visual Manajemen Terhadap Kegiatan Operasional dan Keamanan Laboratorium Biokimia

Management implementation is a form of regulation in conveying information visually with the aim that everyone can understand the situation and conditions in the laboratory area. Visual management implementation uses the 5R principle in its application, namely concise, neat, clean, careful and diligent so as to produce habits that make it a culture to carry out activities in the laboratory according to operational standards. This study aims to determine the level of efficient performance in the operation and safety of the biochemical laboratory. Testing is carried out by identifying the layout of the laboratory interior including tools and materials, planning, compiling, socializing and implementing SOP and K3 in activities in the biochemical laboratory.

Serological Diagnostics Of Atrophy Of The Gastric Mucosa

The association between HP infection and CAH development suggested by the Correa cascade is supported by several cohort studies. To diagnose and determine the severity of atrophy at the Center, patients underwent a serological analysis by ELISA method in the clinical and biochemical laboratory of the State Institution "RSNPMCT and MR". Analyzes were taken in 69 (58.5%) patients, including 35 (50.7%) with CAH, 34 (49.3%) with CNG. Analysis of the non-invasive indicator of the level of pepsinogens in patients with CAH determined that severe atrophy was detected in 20%, moderate atrophy - 42.9%, weak atrophy - 34.3%, and no atrophy was found in 2.9% of patients. The pepsinogen parameters in patients with CNG were also as follows: PG I was within 95.4 ± 7.2 μg / L, PG II 14.5 ± 1.3 and PGI / PGII 6.6 ± 0.2. Pepsinogen values were more pronounced in patients with CAH: with a pronounced degree of atrophy, PGI was 8.7 ± 0.1 μg / l and PGI / PGII was 1.1 ± 0.1; with moderate atrophy 16.6 ± 0.9 μg / l and 1.6 ± 0.1; with mild atrophy 27.2 ± 1.5 and 2.3 ± 0.2, respectively.

GAMBARAN HISTOPATOLOGI PARU PADA MENCIT (Mus musculus) YANG DIPAPAR ASAP ROKOK KRETEK AKIBAT PENGARUH PEMBERIAN INFUSA TEH HIJAU (Camellia sinensis)

This study was conducted to determine the effect of antioxidant administration from green tea infusion (Camellia sinensis) on damage to the histological structure of the lung exposed to clove cigarette smoke in mice (Mus muscullus). Free radicals in cigarette smoke such as tar, nicotine, and carbon monoxide can cause various damages to the lung or This study was conducted to determine the effect of antioxidant administration from green tea infusion (Camellia sinensis) on damage to the histological structure of the lung exposed to clove cigarette smoke in mice (Mus muscullus). Free radicals in cigarette smoke such as tar, nicotine, and carbon monoxide can cause various damages to the lung organs due to oxidative stress caused by the reaction of ultrafine particles with cells. A total of 27 mice were taken from the Biochemical Laboratory of Airlangga University, Surabaya. In this study mice (Mus muscullus) were exposed to kretek cigarette smoke in the smoking chamber for 15 minutes in a period of 2 weeks, used 2 variants of antioxidant doses of green tea infusion of 0.45 gr to 0.6 gr. After the treatment was completed, the preparation of the mice's pulmonary organ preparations and damage to the structure of the lung organ were observed in 9 visual fields. The results showed that there was a relationship between the amount of cigarette smoke exposed and damage to the lung organs of mice when given antioxidant green tea. The administration of green tea antioxidants reduced inflammation of the lung organ infiltration of inflammatory cells by 44%, edema by 55%, and destruction of septa alveoli by 88%. gans due to oxidative stress caused by the reaction of ultrafine particles with cells. A total of 27 mice were taken from the Biochemical Laboratory of Airlangga University, Surabaya. In this study mice (Mus muscullus) were exposed to kretek cigarette smoke in the smoking chamber for 15 minutes in a period of 2 weeks, used 2 variants of antioxidant doses of green tea infusion of 0.45 gr to 0.6 gr. After the treatment was completed, the preparation of the mice's pulmonary organ preparations and damage to the structure of the lung organ were observed in 9 visual fields. The results showed that there was a relationship between the amount of cigarette smoke exposed and damage to the lung organs of mice when given antioxidant green tea. The administration of green tea antioxidants reduced inflammation of the lung organ infiltration of inflammatory cells by 44%, edema by 55%, and destruction of septa alveoli by 88%.

Quality Indicators for the Performance Evaluation at a Biochemistry Laboratory

The use of quality indicators and risk evaluation are valuable tools for maintaining the quality of laboratory tests. There are both requirements of ISO 15189: 2012 and are usually based on common statistical and empirical data. The purpose of the present study was the quality quantification and risk evaluation through the collection, study, and analysis of quality indicators covering the pre-analytical, analytical, and post-analytical phases of the laboratory testing process. Statistical data was collected for the period from 1/12/2017 to 28/2/2018, using the LIS of Biochemical Laboratory. QIs were evaluated using the Six Sigma method and the Pareto statistical tool. FMEA risk analysis was performed, while the degree of risk priority with the Pareto method. The results show that in the analytical phase the QIs give us satisfactory values, while those in the pre- and post- analytical phases need further preventive/corrective actions in order to overcome the problems raised by the QIs. Thus, the fully automatization and computerization of the laboratory is needed.