Characterization of new recombinant Immunoglobulins type-M binding to C1q by Biolayer Interferometry

The Immunoglobulins type-M (IgMs) are one of the first antibody classes mobilized during immune responses against pathogens and tumor cells. Binding to specific target antigens enables the interaction with the C1q complex which strongly activates the classical complement pathway. This biological function is the base for the huge therapeutic potential of IgMs but due to their high oligomeric complexity, in vitro production as well as biochemical and biophysical characterizations are challenging. In the present study, we present new attempts of recombinant production of two IgM models (IgM617 and IgM012) and the evaluation of their polymer distribution using biophysical methods (AUC, SEC-MALLS, Mass Photometry, transmission EM). Each IgM has an individual specific expression yield with different protein quality likely due to intrinsic IgM properties and patterning. Despite the presence of additional oligomeric states, purified recombinant IgMs retain their ability to activate complement in a C1q dependent manner. More importantly, a new method to evaluate their functional quality attribute by characterizing the kinetics of C1q binding to recombinant IgM has been developed using BioLayer Interferometry (BLI). We show that recombinant IgMs possess similar C1q binding properties as IgMs purified from human plasma.

An Essay on Railway Track Bed Failure Issues, Analysis and Remedy

The polymer cures as it enters the ballast, forming a three-dimensional geo-composite reinforcing cage. Although there will be some adherence to the ballast in dry conditions, the polymer's primary job is to construct this reinforcing cage. Polymer penetration is controlled by altering the rheology of the polymer. The method is also said to include a built-in safety system, with the track reverting to a ballast state in the event of a polymer or geo-composite failure. Many of the sites were considered unmaintainable before the polymer was put. The design method was utilized to forecast track behaviour before and after treatment, allowing the most appropriate polymer rheology, polymer distribution, and loading levels to be designed in order to achieve optimum performance and confirm that the procedure worked. This method can be utilized to tackle these types of long-standing problems by displaying actual polymer application profiles at a typical important location.

Mechanism of Post-Radiation-Chemical Graft Polymerization of Styrene in Polyethylene

Structural and morphological features of graft polystyrene (PS) and polyethylene (PE) copolymers produced by post-radiation chemical polymerization have been investigated by methods of X-ray microanalysis, electron microscopy, DSC and wetting angles measurement. The studied samples differed in the degree of graft, iron(II) sulphate content, sizes of PE films and distribution of graft polymer over the polyolefin cross section. It is shown that in all cases sample surfaces are enriched with PS. As the content of graft PS increases, its concentration increases both in the volume and on the surface of the samples. The distinctive feature of the post-radiation graft polymerization is the stepped curves of graft polymer distribution along the matrix cross section. A probable reason for such evolution of the distribution profiles is related to both the distribution of peroxide groups throughout the sample thickness and to the change in the monomer and iron(II) salt diffusion coefficients in the graft polyolefin layer. According to the results of electron microscope investigations and copolymer wettability during graft polymerization, a heterogeneous system is formed both in the sample volume and in the surface layer. It is shown that the melting point, glass transition temperature and degree of crystallinity of the copolymer decreases with the increasing proportion of graft PS. It is suggested that during graft polymerization a process of PE crystallite decomposition (melting) and enrichment of the amorphous phase of graft polymer by fragments of PE macromolecules occurs spontaneously. The driving force of this process is the osmotic pressure exerted by the phase network of crystallites on the growing phase of the graft PS.

Polymer-Bitumen Interaction: A Correlation Study with Six Different Bitumens to Investigate the Influence of SARA Fractions on the Phase Stability, Swelling, and Thermo-Rheological Properties of SBS-PmB

The aim of this work is to determine the influence of the bitumen chemistry on the rheological performance of bitumen and polymer modified bitumen (PmB), as well as the polymer distribution and storage stability. Six different bitumens and their 5 wt.% SBS mixtures are considered in this work. The bitumen composition was determined by SARA fractioning, which was then correlated with the glass transition temperature, complex modulus |G*|, and phase angle, which were obtained by parallel-plate dynamic shear rheology in the temperature range of −25 to 65 °C. The polymer distribution, which was derived from fluorescence microscopy images and the storage stability (determined by tube test) also correlated with the SARA fractions. It was found that the saturates decrease |G*| and Tg and increase the phase angle in crude bitumen, while the asphaltenes increase |G*| and the phase angle. For PmB, the amount of swelling was determined by the saturate content of bitumen. The glass transition temperature of PmBs increases for low saturate and decreases for high saturate contents. |G*| and the phase angle of PmBs correlates with the saturate content, with a varying influence depending on a high or low saturate content and the temperature range due to saturate depletion in the bitumen-rich phase and the varying vol% polymer-rich phase. The aromatic and resin fractions show no correlation in the considered bitumens and PmBs.

Highly sensitive humidity-driven actuators based on metal–organic frameworks incorporating thermoplastic polyurethane with gradient polymer distribution

By combining MIL-88A and thermoplastic polyurethane, a novel humidity-driven actuator was fabricated. The composite films curl from the bottom up, attributed to the uneven vertical gradient distribution of TPU phase. The method promises a new route to humidity actuators.

The Road to Improved Fiber-Reinforced 3D Printing Technology

Three-dimensional printing (3DP) is at the forefront of the disruptive innovations adding a new dimension in the material fabrication process with numerous design flexibilities. Especially, the ability to reinforce the plastic matrix with nanofiber, microfiber, chopped fiber and continuous fiber has put the technology beyond imagination in terms of multidimensional applications. In this technical paper, fiber and polymer filaments used by the commercial 3D printers to develop fiber-reinforced composites are characterized to discover the unknown manufacturing specifications such as fiber–polymer distribution and fiber volume fraction that have direct practical implications in determining and tuning composites’ properties and their applications. Additionally, the capabilities and limitations of 3D printing software to process materials and control print parameters in relation to print quality, structural integrity and properties of printed composites are discussed. The work in this paper aims to present constructive evaluation and criticism of the current technology along with its pros and cons in order to guide prospective users and 3D printing equipment manufacturers on improvements, as well as identify the potential avenues of development of the next generation 3D printed fiber-reinforced composites.

Is It or Isn't It: The Importance of Visual Classification in Microplastic Characterization

Microplastics are a diverse category of pollutants, comprising a range of constituent polymers modified by varying quantities of additives and sorbed pollutants, and exhibiting a range of morphologies, sizes, and visual properties. This diversity, as well as their microscopic size range, presents numerous barriers to identification and enumeration. These issues are addressed with the application of physical and chemical analytical procedures; however, these present new problems associated with researcher training, facility availability and cost, especially for large-scale monitoring programs. Perhaps more importantly, the classifications and nomenclature used by individual researchers to describe microplastics remains inconsistent. In addition to reducing comparability between studies, this limits the conclusions that may be drawn regarding plastic sources and potential environmental impacts. Additionally, where particle morphology data is presented, it is often separate from information on polymer distribution. In establishing a more rigorous and standardized visual identification procedure, it is possible to improve the targeting of complex analytical techniques and improve the standards by which we monitor and record microplastic contamination. Here we present a simple and effective protocol to enable consistent visual processing of samples with an aim to contribute to a higher degree of standardization within the microplastic scientific community. This protocol will not eliminate the need for non-subjective methods to verify plastic objects, but it will standardize the criteria by which suspected plastic items are identified and reduce the costs associated with further analysis.

Development of Test Procedures Based on Chaotic Advection for Assessing Polymer Performance in High-Solids Tailings Applications

Post-thickener polymer addition to initiate rapid tailings dewatering has gained considerable interest for tailings storage facility (TSF) management. However, the highly viscous and non-Newtonian rheology of dense suspensions presents unique challenges for mixing with polymer solutions. Such mixing is highly inefficient, often resulting in polymer overdosing and wide variations in deposited tailings characteristics, with the potential to significantly compromise TSF performance. In this study, a new type of mixer based on the principles of chaotic advection was used for treating kaolin suspensions with high molecular weight (MW) anionic copolymer solutions. Chaotic advection imparts efficient mixing by gently stretching and folding flows in a controlled manner, as opposed to random, high-shear flows associated with turbulent mixing, and this lower shear stress allows for the controlled formation of larger aggregate structures with vastly improved dewatering characteristics. A pre-conditioning pipe reactor prior to this mixer can also be advantageous in terms of providing a short burst of high shear for initial polymer distribution. Seven acrylamide/acrylate copolymers of a fixed anionic charge density (30%) spanning a distinct MW range, as characterized by intrinsic viscosity, were applied at elevated dosages to high-solids (20–30 wt %) kaolin suspensions in continuous flow through the chaotic mixer described above. Medium-to-high MW polymers were generally preferred, with further increases in MW resulting in significantly diminished dewatering outcomes. Direct analysis of polymer solution properties through oscillatory rheology gave a better indication of a polymer’s potential performance compared with intrinsic viscosity, offering a more robust basis for polymer selection. This represented the first systematic study into the effects of polymer properties on deposition behavior after dosing at high solids, which was only possible through the ability to apply controlled shear across the entire suspension during sample preparation.