Occurrence and patterns of nutritional traits and polycyclic aromatic hydrocarbons (PAHs) in sea cucumber (Holothuria polii) tissues: benefits and risk for human health

Objectives The paper evaluated the benefit and risk for human health associated with consumption of sea cucumber H. polii from Italian coasts (Central Mediterranean Sea). Materials and Methods body wall-BW, internal tunic-ITu, muscle bands-MB, alimentary canal-AC, gonad-Gd and respiratory tree-RT of H. polii were analyzed for proximate composition. Moreover, aminoacids, fatty acids and polycyclic aromatic hydrocarbons (PAHs) were determined with HPLC UV/Vis, GC-FID and GC-MS, respectively. Results Differences in the contents of Total Aminoacids (TAA) occurred based on tissue and sex, with AC and MB of female and Gd of male showing higher contents (range 47.8 -60.2 g/kg ww). Glycine and glutamic acid were the most abundant. Polyunsaturated (PUFA) was the major class of fatty acids and Arachidonic and Eicosapentaenoic (EPA) acids were the predominant PUFA. n-3 PUFA showed higher content in Gd, AC and RT indicating a higher quality. A favorable n-3/n-6 in the range 1.04-1.67 was observed. PAHs showed values ranged from 23 to 207 µg/kg ww with the highest levels in Gd-AC tissues and the lower in BW. Benzo[a]Pyrene, the most toxic compound, was detected in all tissues, of both sexes, at levels of 1.5-18 µg/Kg ww. Conclusion All tissues of H. polii, although with differences among them, are valuable food and can contribute for a healthy diet. Excess Cancer risk (CR) values for Gd and AC tissues, were above the considerable CR threshold of one in ten thousand established by USEPA, for high ingestion rate of these seafood.

Bowel cleansing efficacy for colonoscopy: prospective, randomized comparative study of same-day dosing with 1-L and 2-L PEG + ascorbate

Background and study aims Polyethylene glycol (PEG) bowel preparations are effective but associated with high ingestion volume. In this study, 1-L PEG and 2-L PEG preparations were compared in a randomized, colonoscopist-blinded, single-center trial. Patients and methods Patients were aged > 18 years, required colonoscopy, and provided informed consent. Randomization was 1:1 to 1-L PEG or 2-L PEG, based on hospital identification number (odd or even). Preparations were administered using same-day dosing adjusted for colonoscopy start time. The primary endpoint was successful bowel preparation on the Boston Bowel Preparation Scale (BBPS) (no segment scored < 2). Results A total of 852 patients were randomized. In the intention-to-treat (ITT) population, significantly more patients had diabetes in the 2-L PEG arm, resulting in the creation of the modified-ITT population (mITT) that excluded diabetic patients to correct the imbalance (1-L PEG, n = 239; 2-L PEG, n = 238). In the mITT, there was no significant difference in successful cleansing between 1-L PEG and 2-L PEG (88.3 % vs. 82.4 %; P = 0.067). Excellent cleansing (BBPS 7–9; no segment < 2) was significantly improved with 1-L PEG (60.7 % vs. 50.4 %; P < 0.024), as were mean scores in the right and left colon (right: 2.47 vs. 2.30; P < 0.008; left: 2.55 vs. 2.39; P = 0.008). Adverse events were mild to moderate in intensity and none resulted in discontinuation. Rates of nausea and vomiting were significantly higher with 1-L PEG, but that did not affect successful cleansing. Conclusions The lower-volume 1-L PEG was associated with higher levels of excellent bowel cleansing and greater mean segmental scores on the BBPS than 2-L PEG.

Noxic effects of polystyrene microparticles on murine macrophages and epithelial cells

Microplastic (MP) contamination has been identified as an ecological problem with an increasing impact on everyday life. Yet, possible effects of MP at the cellular level are still poorly understood. Here, the interaction of murine macrophages (J774A.1, ImKC) and epithelial cells (STC-1, BNL CL.2) with well-characterized poly(styrene) MP particles (MPP) of varying sizes (0.2–6.0 µm) was studied. Macrophages are expected to actively engulf particles which could be confirmed in this study, while epithelial cells are found in tissues with direct contact with ingested or inhaled MPP. Here, the epithelial cells from both investigated cell lines did not ingest MPP in significant numbers. Concomitantly, no cytotoxic effects nor any influence on cellular proliferation were observed. Cells from the two macrophage cell lines showed high ingestion of MPP of all sizes, but cytotoxic effects were observed only for one of them (ImKC) and only at MPP concentrations above 250 µg/mL. Indications of cellular stress as well as effects on cell proliferation were observed for cell populations with high particle cell interactions.

Microorganisms related to early childhood caries in a sample of an oral preventive-educative program: a longitudinal study

Objetivo: Premature acquisition of cariogenic microorganisms seems to be related to higher prevalence and activity of caries lesions. The aim of the study was to evaluate the prevalence of Streptococcus mutans and Streptococcus sobrinus in infants enrolled in a dental preventive program and in their mothers, as well as to assess the influence of bacterial prevalence, diet and oral hygiene in dental caries prevalence. Material and Methods: After clinical examinations (n = 50), saliva and oral biofilms were collected and stored prior to real-time PCR at 6, 12, 18 and 24 months of age. Results: No correlation was observed between the presence of cariogenic pathogens and diet or hygiene habits at all ages; however, association increased with number of erupted teeth. Salivary levels of bacteria were lower in children than in their mothers at all ages, and children with carious lesions had high ingestion of sugared food. Conclusion: As the levels of cariogenic pathogens were low in the patients that were enrolled in a preventive program, we can conclude that control of oral biofilm as eruption of infants’ teeth occurs and sugar ingestion should be considered of great importance in preventive dentistry, because the association between them was highly positive.KeywordsSaliva; Dental caries; Oral hygiene; Cariogenic; Streptococcus mutans.

Serum and liver copper, iron, molybdenum and zinc concentration in goats and sheep in the state of Paraíba, Brazil

ABSTRACT: This study aimed to determine Cu, Fe, Mo, and Zn liver and serum concentration in sheep and goats raised in the rangelands of the semiarid region of the state of Paraíba, Brazil, during the dry and rainy seasons, and to establish if Cu deficiency is primary or secondary to high ingestion of Mo or Fe. Cu, Zn, Mo and Fe concentrations were determined by atomic absorption spectrometry coupled to mass (ICP-Plasma) in 253 liver and serum samples randomly selected in a slaughterhouse. The mean serum concentrations of Cu in the goats and sheep were 11.82±3.28μmol/L and 10.97±3.61μmol/L respectively. The liver Cu concentrations were 160.37±11.77mg/kg in goats and 152.12±13.16mg/kg in sheep. The mean serum Fe concentrations were 16.38±4.51μmol/L in goats and 25.41±9.76μmol/L in sheep. The mean Fe concentrations in the liver were 189.37±6.51mg/kg in goats and 313.70±12.89mg/kg in sheep. The mean serum concentrations of Mo were 0.14±0.04μmol/L in goats and 0.29±0.06μmol/L in sheep. The mean Mo concentrations in the liver were 6.09±0.23mg/kg in goats and 6.22±0.15mg/kg in sheep. The mean serum Zn concentrations were 8.30±1.91μmol/L in goats and 8.63±2.22μmol/L in sheep. The mean Zn concentrations in the liver were 132.80±3.39mg/kg in goats and 130.70±2.99mg/kg in sheep. These results show low or marginal serum and liver concentrations of Cu and Zn, indicating that these minerals should be supplemented. The normal or even low concentrations of Mo and the high concentration of Fe suggest that Cu deficiency may be primary, due to low Cu ingestion, or secondary due to high Fe ingestion.

The role of the sugar receptor IR60b for Drosophila melanogaster: A hypothesis

In a recent paper, Joseph and colleagues (Joseph et al., 2017) have characterized the selective sucrose receptor IR60b in Drosophila, and proposed that it serves to limit sucrose consumption, and thus to prevent overfeeding. Here, we propose an alternative hypothesis for this sucrose receptor. Adult fruit flies feed by excreting saliva onto the food, and imbibing the predigested liquefied food, or by filling the crop, where the food is predigested. Enzymes in the saliva hydrolyse starch and disacharides into absorbable monosacharides. Premature ingestion into the midgut would not give the enzymes in the saliva enough time to predigest the food. Thus, IR60b might be used as a sensor to monitor the digestive state of external food or crop content: when disaccharides (sucrose) concentration is high, ingestion is inhibited, preventing the malabsorption of sucrose in the gut.

The role of the sugar receptor IR60b for Drosophila melanogaster: A hypothesis

In a recent paper, Joseph and colleagues (Joseph et al., 2017) have characterized the selective sucrose receptor IR60b in Drosophila, and proposed that it serves to limit sucrose consumption, and thus to prevent overfeeding. Here, we propose an alternative hypothesis for this sucrose receptor. Adult fruit flies feed by excreting saliva onto the food, and imbibing the predigested liquefied food, or by filling the crop, where the food is predigested. Enzymes in the saliva hydrolyse starch and disacharides into absorbable monosacharides. Premature ingestion into the midgut would not give the enzymes in the saliva enough time to predigest the food. Thus, IR60b might be used as a sensor to monitor the digestive state of external food or crop content: when disaccharides (sucrose) concentration is high, ingestion is inhibited, preventing the malabsorption of sucrose in the gut.

Major constrains of the pelagic food web efficiency in the Mediterranean Sea

Grazing pressure plays a key role on plankton communities affecting their biodiversity and shaping their structures. Predation exerted by 2–200 μm protists (i.e. microzooplankton and heterotrophic nanoplankton) influences the carbon fate in marine environments channeling new organic matter from the microbial loop toward the "classic" grazing food web. In this study, we analyzed more than 80 dilution experiments carried out in many Mediterranean sites at the surface and in the meso-bathypelagic layers. Our aims were to investigate prey-predator interactions and determine selectivity among energy sources (in terms of available biomass), efficiency in the exploitation and highlight likely constrains that can modulate carbon transfer processes within the pelagic food webs. Generally, microzooplankton shown higher impacts on prey stocks than heterotrophic nanoflagellates, expressing larger ingestion rates and efficiency. Through different trophic conditions characterized on the base of chlorophyll a concentration, microzooplankton diet has shown to change in prey compositions: nano- and picoplankton almost completely covered consumer needs in oligotrophy and mesotrophy, while microphytoplankton (mostly diatoms) represented more than 80% of the consumers' diet in eutrophy, where, nevertheless, picoplankton mortality remained relatively high. Ingestion rates of both consumers (nano- and microzooplankters) increased with the availability of prey biomasses and consequently with the trophic condition of the environment. Nevertheless, overall the heterotrophic fraction of picoplankton resulted the most exploited biomass by both classes of consumers. Ingestion efficiency (as the ratio between available biomass and ingestion rate) increased at low biomasses and therefore the highest efficiencies were recorded in oligotrophic conditions and in the bathypelagic layers.