People suffer from stress or digital stress when using information communication technology. However, few tools have been developed to capture this phenomenon. The concept of digital stress has remained controversial. This preregistered study aims to reflect and clarify the concept of digital stress and to develop a digital stress scale for adolescents. This study integrated qualitative and quantitative approaches to explore the structure of digital stress and develop a digital stress scale. First, we theoretically proposed the digital stress item pool based on current theories of digital stress and the definition of digital stress. Then, we conducted focus groups and in-depth interviews (N=41) to revise the items in the item pool. Lastly, we finalized the scale based on the data collected from three independent samples of adolescents (Nsample1=1088, Nsample2=879, Nsample3=176). The results revealed six dimensions of digital stress among adolescents: unsatisfactory information and communication, unmet recreational motivation, online learning burden, social concerns, useless and overloaded notifications, and online verbal attacks. The scale showed robust reliability (2=.851 to .959), stability (test-retest reliability =.717 to .681, p < .001), and validity (construct validity: X2= 2107.978, df = 387, CFI = .932, TLI = .923, RMSEA = .064; correlation with anxiety and depression is .431 to .462, p < .001). The developed scale is reliable in measuring digital stress in adolescents, especially when few measures exist. Limitations, implications, and guidance for future research are discussed.