scholarly journals Production of Renewable Lipids by the Diatom Amphora copulata

Fermentation ◽  
2021 ◽  
Vol 7 (1) ◽  
pp. 37
Author(s):  
Natanamurugaraj Govindan ◽  
Gaanty Pragas Maniam ◽  
Mohd Hasbi Ab. Rahim ◽  
Ahmad Ziad Sulaiman ◽  
Azilah Ajit ◽  
...  
Keyword(s):  
Coastal Waters ◽  
Palmitoleic Acid ◽  
Biomass Concentration ◽  
Dry Weight ◽  
Dry Mass ◽  
Dark Cycle ◽  
Batch Cultures ◽  
Palm Oil Mill ◽  
Optimized Conditions ◽  
Tetracosanoic Acid

The asymmetric biraphid pennate diatom Amphora copulata, isolated from tropical coastal waters (South China Sea, Malaysia), was cultured for renewable production of lipids (oils) in a medium comprised of inorganic nutrients dissolved in dilute palm oil mill effluent (POME). Optimal levels of nitrate, phosphate, and silicate were identified for maximizing the biomass concentration in batch cultures conducted at 25 ± 2 °C under an irradiance of 130 µmol m−2 s−1 with a 16 h/8 h light-dark cycle. The maximum lipid content in the biomass harvested after 15-days was 39.5 ± 4.5% by dry weight in a POME-based medium with optimal levels of nitrate, phosphate, and silicate. Under the optimized conditions the maximum dry mass concentration of the diatom was 660 mg L−1 on day 12, declining to ~650 mg L−1 on day 15. For the 15-day batch operation, the final average productivities of the biomass and the lipids were 43.3 ± 4.5 mg L−1 d−1 and 17.1 ± 0.3 mg L−1 d−1, respectively. The fatty acids in the diatom lipids were found to be (%, w/w of total lipids): palmitoleic acid (39.8%), palmitic acid (31.9%), myristic acid (6.8%), oleic acid (4.7%), stearic acid (4.5%), arachidonic acid (3.9%), eicosapentaenoic acid (3.6%), linoleic acid (2.5%), tetracosanoic acid (1.7%), and linolenic acid (0.6%).

STEM measurement of subcellular water distributions

1993 ◽  
Vol 51 ◽  
pp. 568-569
Author(s):  
R.D. Leapman ◽  
S.Q. Sun ◽  
S-L. Shi ◽  
R.A. Buchanan ◽  
S.B. Andrews
Keyword(s):  
Water Content ◽  
Internal Standard ◽  
Dry Weight ◽  
Dry Mass ◽  
Scanning Transmission Electron Microscope ◽  
Dark Field ◽  
Bulk Water ◽  
Inelastic Electron ◽  
Scanning Transmission ◽  
Annular Dark Field

Recent advances in rapid-freezing and cryosectioning techniques coupled with use of the quantitative signals available in the scanning transmission electron microscope (STEM) can provide us with new methods for determining the water distributions of subcellular compartments. The water content is an important physiological quantity that reflects how fluid and electrolytes are regulated in the cell; it is also required to convert dry weight concentrations of ions obtained from x-ray microanalysis into the more relevant molar ionic concentrations. Here we compare the information about water concentrations from both elastic (annular dark-field) and inelastic (electron energy loss) scattering measurements.In order to utilize the elastic signal it is first necessary to increase contrast by removing the water from the cryosection. After dehydration the tissue can be digitally imaged under low-dose conditions, in the same way that STEM mass mapping of macromolecules is performed. The resulting pixel intensities are then converted into dry mass fractions by using an internal standard, e.g., the mean intensity of the whole image may be taken as representative of the bulk water content of the tissue.

scholarly journals AVOCADO SEEDLINGS MULTIPLE STEMS PRODUCTION

2016 ◽  
Vol 38 (2) ◽  
Author(s):  
MARCUS VINICIUS SANDOVAL PAIXÃO ◽  
JOSÉ CARLOS LOPES ◽  
EDILSON ROMAIS SCHMILDT ◽  
RODRIGO SOBREIRA ALEXANDRE ◽  
CAROLINE MERLO MENEGHELLI
Keyword(s):  
Root Length ◽  
Dry Weight ◽  
Adventitious Rooting ◽  
Dry Mass ◽  
Stem Diameter ◽  
Experimental Unit ◽  
Shoot Height ◽  
Shoot Dry Weight ◽  
Federal Institute ◽  
Multiple Stems

ABSTRACT This study aimed to evaluate the potential of multi-stems in avocado seeds according to their mass as well as the adventitious rooting of multi-stem budding with or without the use of auxin. The research was carried out at the Vegetation House of Federal Institute of Espírito Santo, Campus Santa Teresa -ES, with seeds of different masses: <60 g, 61 to 80 g, 81 to 100 g and >100 g, in which each experimental unit was made of five seeds, distributed within five repetitions, under a completely randomized design. The seeds were put to germinate and the percentage number of emergence and multiple stems were evaluated. After 150 days, the following evaluations were carried out: survival of rooted cuttings; number of leaves; stem diameter; root length; root volume; root and shoot fresh mass; root and shoot dry mass; shoot height; absolute growth and shoot growth rate; shoot dry weight/root dry mass ratio; shoot height/stem diameter ratio; shoot height/root length and Dickson's quality index ratio. Avocado seeds with mass over 100 g and between 81-100 g presented higher percentage of multiple stems. Rods over 20 cm that were not treated with IBA (indole-3-butyric acid) resulted on avocado plants of better quality. The use of IBA (2000 mg L-1) does not affect the rooting and growth of avocado's multi-stem plants.

scholarly journals MICROALGAE FROM THE COASTAL WATERS OF TYULENY ISLAND IN THE CASPIAN SEA

2019 ◽  
Vol 14 (2) ◽  
pp. 120-131
Author(s):  
R. M. Barkhalov ◽  
A. A. Abdurakhmanova ◽  
F. Sh. Amaeva
Keyword(s):  
Coastal Waters ◽  
Caspian Sea ◽  
Biomass Concentration ◽  
Water Area ◽  
Trophic Chain ◽  
The Caspian Sea ◽  
Standard Procedures ◽  
Water Surface Layer ◽  
Rich Flora ◽  
Positive Effect

Aim. In this work, we set out to study the composition of a phytoplankton community in an important fishery area, the coastal water area of Tyuleny Island in the Caspian Sea.Methods. We present the results of seasonal observations (2016) on the state of phytoplankton in the coastal waters of Tyuleny Island in the Caspian Sea. In total, 120 phytoplankton samples were collected at four stations from the water surface layer (May–October) using the Nansen bottle and subsequent fixation by Lugolʹs solution. Sedimentation and concentration were carried out using standard procedures. The samples were processed in the Nageotte chamber with a volume of 0.1 ml under a light microscope.Results. According to the research results (2016), 103 species and varieties of microalgae were found in the phytoplankton samples collected from the water area of Tyuleny Island. The microalgae were represented by four divisions: Bacillariophyta – 49 species, Cyano‐ phyta – 24 species, Chlorophyta – 23 species and Pyrrophyta – 7 species. The greatest species diversity of phytoplankton in the studied water area was noted during the autumn period (61 spe‐ cies). In general, phytoplankton was found to be distributed homogeneously throughout the coastal area of the island, with the biomass concentration not reaching 1 g/m3.Conclusion. In 2016, favorable hydrological and hydrochemical conditions for the development of microalgae were observed. The desalinated water around Tyuleny Island, which is well warmed in the summer and does not freeze in the winter, contributed to the development of rich flora. Although bi‐ omass values were not high due to the prevalence of small‐celled microalgae in phytoplankton, in general, it should have a positive effect on the development of subsequent links in a trophic chain, as well as promote an increase in the productivity of waters of the Northern Caspian Sea. 

scholarly journals KOMPOSISI ASAM LEMAK CACING LAUT SIASIA (SIPUNCULUS, SP) DARI PERAIRAN PANTAI PULAU NUSALAUT

2017 ◽  
Vol 4 (1) ◽  
pp. 10-16
Author(s):  
Bernita Silaban
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Stearic Acid ◽  
Eicosapentaenoic Acid ◽  
Lauric Acid ◽  
Coastal Waters ◽  
Myristic Acid ◽  
Palmitoleic Acid ◽  
Fat Content ◽  
Coastal Communities

Background: "Siasia" is a seaworm species in the phylum that includes Sipuncula Sipunculidea class. This animal has been consumed for generations by coastal communities Nusalaut Island, central mollucas but not yet universally known. Until now there has been obtained gisi complete composition. This study aimed to identify the composition of fatty acids contained in vain fresh seaworms. Methods: Seaworms vain taken from coastal waters of Negeri Titawaai and Nalahia Nusalaut Island, Central Moluccas in March 2014. The parameters analyzed include methods is sokhlet fat content and fatty acid by GC method. Results: The results showed fresh siasia fat content 1.12% of coastal waters Titawaai while 1.91% of coastal waters Nalahia. Fatty acids seaworms were identified from coastal waters Titawai  is  kaparat acid (C10: 0), lauric acid (C12: 0), myristic acid (C14: 0), palmitoleic acid (C16: 1), stearic acid (C18: 0), linolenic acid (C18: 3) acid and eicosapentaenoic (C20: 5) while the fatty acids of  seaworm vain of coastal waters Nalahia include is lauric acid (C12: 0), myristic acid (C14: 0), palmitoleic acid ( C16: 1), stearic acid (C18: 0) and eicosapentaenoic acid (C20: 3). Conclusion: The fat content of fresh siasia sea worms is 1.12% from the waters of Titawaai beach, while 1.91% of the waters of the coast of Nalahia.

Regulation of growth and differential tissue dry mass accumulation by Citrus grandis, Poncirus trifoliata and their F1 under salinized and non-salinized environments

2000 ◽  
Vol 27 (1) ◽  
pp. 27 ◽  
Author(s):  
Ilhami Tozlu ◽  
Gloria A. Moore ◽  
Charles L. Guy
Keyword(s):  
Salinity Tolerance ◽  
Mass Production ◽  
Fine Root ◽  
Dry Weight ◽  
Dry Mass ◽  
Poncirus Trifoliata ◽  
Initial Growth ◽  
Ion Content ◽  
Average Growth ◽  
Leaf Tissues

Salt stress responses of C. grandis L. (Osb.), P. trifoliata (L.) Raf. and their F 1 were investi-gated. Growth, growth rates, as well as leaf, stem, structural root (> 2 mm diameter), fine root (≤ 2mm diameter) and whole plant dry masses were determined for the three genotypes tested in 0, 40 and 80 mМ NaCl environments for 20 weeks. P. trifoliata and C. grandis were phenotypically distinct and their F1 had features that were a combination of both parents. The different growth habits resulted in significant differences between the net growth and growth ratios ([net growth / initial growth] × 100) of the three genotypes and between control and salinized plants within each genotype. The average growth and dry weights of nearly all tissues were reduced in salinized plants compared to those of control plants. The exceptions were the fine roots of P. trifoliata at both salinities and of the F1 plants at 40 mМ NaCl. The 40 mМ NaCl treatment stimulated fine root production in P. trifoliata plants, significantly increasing dry weight by 30% compared to control plants. Average shoot dry weight reduction was greatest in C. grandis and least in P. trifoliata in the 40 mМ NaCl treatment. While leaf tissues of P. trifoliata were the most sensitive to salinity, root tissues were the most sensitive in C. grandis. To avoid salt accumulation, P trifoliata plants increased root dry mass production while C. grandis plants increased leaf mass production. These traits appeared to be heritable, since the F1 plants displayed responses intermediate to its parents leading to increased salinity tolerance. We suggest that not only ion content of leaf tissues, but ion content and mass production of all tissues should be considered when the salinity tolerance of Citrus and related genera is characterized.

scholarly journals PRODUÇÃO DE MUDAS DE Physocalymma scaberrimum EM SUBSTRATOS COMPOSTOS POR DIFERENTES PORCENTAGENS DE RESÍDUO ORGÂNICO DE AÇAÍ

FLORESTA ◽  
2012 ◽  
Vol 42 (2) ◽  
pp. 399 ◽  
Author(s):  
Álisson Sobrinho Maranho ◽  
Ary Vieira de Paiva
Keyword(s):  
Native Species ◽  
Urban Forestry ◽  
Dry Weight ◽  
Dry Mass ◽  
Native Forest ◽  
Shoot Height ◽  
The North ◽  
Randomized Design ◽  
Collar Diameter ◽  
Growth Of Seedlings

Physocalymma scaberrimum Pohl é uma espécie nativa que ocorre principalmente no Norte e Centro-Oeste do Brasil, com potencial madeireiro, para arborização urbana e para recomposição de vegetação em áreas degradadas. Considerando a necessidade de conhecimento do seu comportamento em viveiro e da utilização de resíduos agroflorestais como componente de substrato, este estudo teve por objetivo avaliar o crescimento de mudas dessa espécie em substratos compostos por porcentagens de resíduo de açaí (0%, 25%, 50%, 75%, 100%) misturado à terra de mata. O experimento ocorreu em delineamento inteiramente casualizado, com 5 tratamentos (substratos) e 20 repetições. Foi avaliado o crescimento das mudas através da altura, diâmetro do colo, massa seca da parte aérea e radicular e total, e Índice de Qualidade de Dickson (IQD). Houve ajuste de regressão linear e correlação positiva entre altura, diâmetro do colo e massa seca total com as porcentagens de resíduo de açaí. O substrato constituído por 100% do resíduo de açaí proporcionou o maior crescimento das mudas: 32,9 cm de altura, 2,98 mm de diâmetro do colo, 2,003 g de massa seca total e maior IQD (0,1142). Para a utilização com terra de mata, a composição entre 25% e 75% de resíduo também se mostrou possível.Palavras-chave: Resíduos agroflorestais; produção de mudas; espécies florestais nativas; silvicultura. AbstractPhysocalymma scaberrimum seedlings production in substrate composed by different percentages of organic residue of açaí. Physocalymma scaberrimum Pohl is a native species that occurs mainly in the North and Midwest regions of Brazil, with potential for wood production, urban forestry and restoration of vegetation in degraded areas. Considering the need for better understanding of the seedlings behavior in the nursery, and use of agroforestry residue as components of substrate, this study aimed to evaluate the growth of this species in substrates composed by different percentages of açaí residue (0%, 25%, 50%, 75%, 100%) mixed with forest soil. The experiment was set up according to the completely randomized design, with 5 treatments (substrates) and 20 replicates. It was evaluated the growth of seedlings through shoot height, collar diameter, dry weights of shoot and roots and total, and Dickson Quality Index (IQD). A fit linear regression and positive correlation was found out between height, collar diameter and dry weight total dry mass with increasing percentages of açaí residue. The substrate composed by 100% of açaí residue provided the highest mean growth of seedlings: height (32.9 cm seedling-1), collar diameter (2.98 mm seedling-1), total dry mass (2.003 g seedling-1), and highest IQD (0.1142). To use with soil, the composition between 25% and 75% of residue also showed possibility.Keywords:agroforestry residues; seedlings production; native forest species; silviculture.

Effect of ammonium on growth of plant cells (Ipomoea sp.) in suspension cultures

1975 ◽  
Vol 53 (17) ◽  
pp. 1942-1949 ◽  
Author(s):  
Dyson Rose ◽  
S. M. Martin
Keyword(s):  
Nitrogen Content ◽  
Dry Weight ◽  
Second Phase ◽  
Growth Phases ◽  
High Nitrogen Content ◽  
High Nitrogen ◽  
Batch Cultures ◽  
High Nitrogen Level ◽  
Ammonium Level ◽  
Two Phases

Maintenance of the ammonium level in batch cultures of a plant cell line initiated from Ipomoea root tissue resulted in cells with a higher nitrogen content and a greater total accumulation of nitrogen in the cells. Utilization of nitrate was not suppressed. The increased supply of ammonium did not, by itself, increase the yield of dry cells, but it did permit a twofold or greater increase in cell yield when sucrose was added subsequently.Supplementary feeding of ammonium and sucrose to a batch culture that had exhausted the initial supply of ammonium rapidly returned the cells to a state of high nitrogen content, favored further utilization of nitrate, and greatly increased cell yield.These results are discussed in terms of growth phases. The presence of ammonium in the medium appeared to induce and maintain a growth phase characterized by a high nitrogen level in the cells. After the ammonium supply was depleted a second phase occurred, characterized by a rapid increase in dry weight without proportional accumulation of nitrogen. This second phase was not caused by a limiting rate of nitrate utilization, nor was it directly related to observed pH changes. We conclude that the two phases relate to the presence and absence of ammonium in the medium.

Parameters for growth measurement in suspension cultures of plant cells

1974 ◽  
Vol 52 (4) ◽  
pp. 903-912 ◽  
Author(s):  
Dyson Rose ◽  
S. M. Martin
Keyword(s):  
Stationary Phases ◽  
Plant Cells ◽  
Growth Period ◽  
Dry Weight ◽  
High Rate ◽  
Growth Phases ◽  
Batch Cultures ◽  
Growth Measurement ◽  
Large Increments ◽  
Uptake And Metabolism

Well-adapted cells, which had been initiated from root tissue of Ipomoea and of Daucus carota, were grown in 7.5-liter stirred-jar fermenters, and both the cells and media were analyzed for major components at intervals during the growth period. Controlled variables included the size of inoculum, the amount of sucrose, and the source and amount of nitrogen in the media.The data obtained indicate that there are two distinct growth phases in the development of batch cultures of these cell lines. The first, which we term "cytoplasmic growth phase," begins immediately upon addition of inoculum to fresh medium and is characterized by a high rate of nitrogen uptake and metabolism relative to the increase in cell dry weight. The second, or "maturation phase," is characterized by large increments in dry weight and total cell carbohydrate relative to the increments in cell nitrogen. It is suggested that the classical lag, log, and stationary phases of bacterial growth could apply only to the early hours of cytoplasmic growth, if indeed they are relevant at all.

scholarly journals Influence of NaCl Salinity and Different Substracts on Plant Growth, Mineral Nutrient Assimilation and Fruit Yield of Strawberry

2011 ◽  
Vol 39 (2) ◽  
pp. 219 ◽  
Author(s):  
Asghar RAHIMI ◽  
Ali BIGLARIFARD
Keyword(s):  
Plant Growth ◽  
Leaf Area ◽  
Inorganic Ions ◽  
Dry Weight ◽  
Dry Mass ◽  
Fruit Yield ◽  
Mineral Nutrient ◽  
Nutrient Assimilation ◽  
Concentration Changes ◽  
Total Dry Weight

A hydroponic culture was carried out with strawberry cv. Camarosa to investigate the effects of four salinity levels and four different substrates on plant growth, mineral nutrient assimilation and fruit yield of strawberry. Total dry weight accumulation of plants was not inhibited at low salinities, but it was significantly inhibited at 60 mM NaCl. Dry mass (DM) partitioning in NaCl-stressed plants was in favor of crown and petioles and at the expense of root, stem and leaf, whereas leaf, stem and root DM progressively declined with an increase in salinity. Specific leaf area (SLA) and leaf area ratio (LAR) significantly decreased in cv. Camarosa at 60 and 90 mM. Results also showed that the presence of NaCl in the root medium induced an increase in total Na+ content of the plants in the shoot and root. Despite Na+ and K+, the increase in total inorganic ions resulted from increasing salinity, with Ca2+ and Mg2+ concentrations decreasing in shoot and increasing in roots with an increase in salinity. For all micro- and macroelements however, significant concentration changes related to different substrates were not detected in the present experiments. Results also showed a significant decline of Fe content of 40% and 49% in shoot and root, respectively.

Effect of EGCG Application on Collagen Degradation in Dentine Caries

2013 ◽  
Vol 455 ◽  
pp. 112-116 ◽  
Author(s):  
Huang Qin Chen ◽  
Bin Huang
Keyword(s):  
Mass Loss ◽  
Storage Time ◽  
Artificial Saliva ◽  
Acetic Acid Solution ◽  
Dry Weight ◽  
Collagen Matrix ◽  
Dry Mass ◽  
Collagen Degradation ◽  
Experimental Media ◽  
Dentin Matrix

Caries progress might be controlled when collagen matrix could be preserved after demineralization. The aim of this study was to investigate the effect of EGCG, a major polyphenol of green tea, on changes in loss of dry weight or solubilization of collagen from demineralized dentin sections. Dentin sections (1mm×5mm×5mm) were completely exposed to the acetic acid solution to form dentine caries. After baseline measurements of dry mass, the samples were divided into 2 groups and incubated at 37°C in either media containing EGCG or artificial saliva only for 24h, 3 days, 1 week, 2 week and 3 weeks. Following hydrolyzed with HCl, hydroxyproline (HOP) as an index of solubilization of collagen was analysed quantitatively. Both the storage medium and the storage time showed significant effects on mass loss and HOP release (p < 0.05). The incubation in EGCG resulted in relatively rapid and significant (p < 0.05) decrease in the amounts of mass loss. The HOP content of the experimental media also increased with incubation time but was significantly lower (p < 0.05) than in the control artificial saliva medium. It can be concluded that EGCG can inhibit collagen degradation and affects the further demineralization in dentin matrix, which consequently inhibits the advanced dentine caries.

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