Study and development of methods for obtaining intergeneric hybrids of salmonids (Salmonidae (Jarocki or Schinz, 1822)) for achieving the effect of heterosis and increasing their productivity

Purpose. To study the possibility of obtaining highly productive intergeneric salmonid hybrids between rainbow trout and brook trout; brown trout and brook trout; rainbow trout and brown trout, as well as to develop methodological approaches and determine of optimal variants of hybrid crosses. Findings. In order to obtain intergeneric hybrids, we used six variants of hybrid crosses with brood fish of three salmonids belonging to three families (Salmo, Oncorhynhus, Salvelinus). The study used age-4 female rainbow trout with average body weight of 3296.8 g, Fork length was 62.6 cm, and the average working fecundity was 7420 eggs. Age-3 rainbow trout males had an average body weight of 1613 g and an average body length of 49.8 cm; age-3 brown trout females had an average body weight of 453.8 g and average working fecundity of 1540 eggs, and males had an average weight of 458.7 g; age-3 brook trout females had an average weight of 809.7 g and a length of 38.9 cm with working fecundity of 1732 eggs, and age-4 males had an average weight of 1212.8 g and an average body length of 46.0 cm. Twelve variants of fertilization were used: six variants at normal water temperature and six variants after a temperature shock. Under natural conditions, the creation of intergeneric hybrids is almost impossible, except for variants between brown trout and brook trout, which is due to the similarity of their biology. However, the efficiency of this cross is low and economically impractical for fish farmers. When applying the temperature shock during fertilization, hybrids proved to be the most effective, where females were rainbow trout, and males were brook trout and brown trout. The average weight of young-of-the-year intergeneric hybrids was, depending on the species of fish, from 8 to 54 g. The highest results were obtained for the creation of hybrids where following broodstock was used: ♂brook trout Х ♀brown trout; ♂brown trout Х ♀rainbow trout. In these variants of crossbreeding, the survival rate of young-of-the-year during the period of cultivation was 94.8 and 92.8%, respectively. In particular, the above hybrids did not suffer from infectious diseases during the growing period. Originality. New data on the development of methods for obtaining viable offspring of newly created hybrids were obtained, and the optimal variants of crossing between females and males of these salmonids were determined. Practical value. The results can be used for artificial breeding of salmonids in specialized farms that will allow obtaining high quality products and reducing their costs. Key words:rainbow trout, brown trout, brook trout, incubation, free embryos, larvae, fry, young-of-the-year.

Body measurements of Black Slavonian pig

The Black Slavonian pig is an autochthonous pig breed in Croatia, which has recorded a continuous growth of the population followed by the higher number of breeders in recent years. The increase in population has removed the Black Slavonian breed from the category of endangered local breeds. The consequences of such a significant increase in population size in local breeds are often a high level of inbreeding, but also a high variability of the external traits of breeding individuals. Given that the main goal of the Breeding Program for Black Slavonian pigs is to preserve its phenotypic traits and breed-specific features, the paper presents the results of external traits analysis at 10 different points on the body, on a sample of 102 animals, aged between 10 and 24 months. Estimated mean values for wither’s height were 65 cm and 64 cm in boars and sows, respectively. Average body length was 128 cm for boars, and 126 cm for sows. The heart girth was 114 cm for both categories, while the height at the sacrum was 72 cm (boars), and 71 cm (sows). These results indicate very small or insignificant differences between male and female individuals, and refer that the body measurements of Black Slavonian pigs have not changed significantly in relation to its formation and development over time, as well as in relation to other local breeds from the neighbouring regions. With this in mind, selection work should be focused on control and preservation. Furthermore, the paper gives an overview of the population throughout history, as well as breeding practices.

BIOLOGY AND ECOLOGICAL ADAPTATIONS OF BLACKBUCK (Antilope cervicapra): A REVIEW

Blackbuck is the existing members of genus Antilope which is most elegant and graceful among all Antelopes of Asia with distinct sexual dimorphism. Blackbuck show endemism in Pakistan, Nepal and India occupying mainly the semi-arid grassland areas. The name of species attributes towards the dark brownish to blackish coat color of male species. While female and the young ones are tawny or yellow. Chin undersides of legs and chest are white in both male and female individuals. Males have whorled horns with 79cm length with absence in females. The average body length of this animal is 100-150cm with the tail length of 10-17cm. The average body weight for male is 20-57kg and for female 19-33kg. Thin grassy forests, open and semi-desert areas are good habitat for it. Being diurnal and herbivorous, it acts as both grazers and browsers. It is the fastest animal with an average speed of 80km/h. It remains reproductively functional whole year. Reproductive disorders including dystocia and different infectious diseases due to ectoparasites, endoparasites, bacteria and viruses affect these species. Habitat loss, stress, illegal killing and genetic troubles cause the species to be ‘Extinct in the Wildlife’ in Pakistan so conservation strategies are underway for species protection. It is indispensable for zoologists and conservational biologists to observe the species for its conservation and confronting threats. Review article highlighted necessary information about species, which will clear the way for further research on species.

EFFECT OF PROBIOTIC SIBMOS PRO ON THE GROWTH OF JUVENILE ALTAI MIRROR CARP IN POND FARMING

The influence of the probiotic SibMOS PRO on the growth of juveniles of the Altai mirror carp in the conditions of pond farming was studied. Antimicrobial feed concentrate SibMOS PRO is made on the basis of mannanoligosaccharides (derived from yeast cell walls) in combination with bacteria Bacillus subtilis and Bacillus licheniformis. Studies were carried out to change the morphological characteristics, as well as the growth rate of juveniles of the Altai mirror carp when introducing feed probiotics in the feed and in the water of outgrowth ponds. The presence of feed probiotics in fish feed had a positive impact on the growth dynamics of juvenile Altai mirror carp. Probiotics added directly to the water had a positive effect on the growth and development of zooplankton, thereby increasing its number in the reservoir. At the end of the growing period, the greatest average body length of juveniles was observed in ponds where the fish were fed with probiotic feed. Adding a probiotic to the water had little effect on the growth of the fish. The highest fatness coefficient was in the juveniles of the Altai mirror carp, who received probiotics in the feed.

Osteometric Study of an Early Iron Age Postcranial Sample from Lower Kama Region (Novo-Sasykul Burial Ground).

The article outlines results of an osteometric study of a postcranial sample (22 male and 30 female skeletons) from Novo-Sasykul burial ground in the Lower Kama region (Pyany Bor culture). Novo-Sasykul necropolis dates back to between the I and the turn of the II–III cc. AD. In total, the Sasykul population is characterized by a mesomorphic (with a tendency to dolichomorphism) body type proportions and an average or higher than average body length. Results of intragroup analysis allow to conclude that the studied sample was mixed. Results of the intergroup canonical discriminant analysis demonstrate the greatest proximity of the Novo-Sasykul postcranial skeletons to the Mazunino culture samples (Pokrovsky, Dubrovsky and Boyarsky “Arai” burial grounds), as well as to the Pyany Bor culture sample from Stary Chekmak cemetery. The latter thesis does not apply to female skeletons from Stary Chekmak, characterized by a rather “harmonious” ratio of the limb segment lengths and do not show a tendency towards relative lengthening of the tibia. A slight increase in the crural index may be a specific feature of the Pyany Bor and Mazunino culture population groups from the Kama region.

SOME DATA ON BIOLOGY AND FISHERY OF ICEFISH SALANGICHTHYS MICRODON IN PETER THE GREAT BAY (JAPAN SEA)

Within Peter the Great Bay, common icefish Salangichthys microdon forms commercially valuable aggregations in two areas of the Amur Bay and in one area of the Ussuri Bay. It spawns in the internal estuaries of the rivers entered to the bays and in the external estuarine zones in the tops of the bays, mostly on small pebble grounds from late April to late May and feeds in the bays waters. Data on its size and weight composition were obtained in 2017–2018 both from the Amur Bay (for spawning period) and from the Ussuri Bay (for feeding period). During the spawning (on May 6 and May 18), the body length was 59–90 mm, on average 74.9 mm, and the weight was about 750 mg; sex ratio males : females was 1.0 : 0.8. During the feeding, the average body length was 66.8 mm.

PENGAMATAN PENDAHULUAN OVARIUM BANDIKUT (Echymipera kalubu)

The study aimed to determine the size and physical description (location, shape, colour and texture) ovarian of Echymipera kalubu. Study was conducted in 3 months from June to September 2017. Three female E. kalubu with an average body weight 399±97.32g and average body length 21.67±5.51cm were used in this study. Sample of bandicoots were dissected using a surgical tool and then measured using measuring tester and analytic scales. Descriptive statistics was used to analyse the data. The results showed that average length of female reproductive organs of E. kalubu was 11,00±0,87 cm (right) and 11,03±0,83 cm (left); average weight of ovarian was 0,01±0,0048 g (right) and 0,02±0,0006 g (left). This study also showed that characteristics of ovarian were in common with other polytocus animals.

Morphometric and carcass characteristics of Jaunpuri breed of goats

The present study was undertaken to observe morphometric and carcass characteristics of Jaunpuri goat. The survey plan was conducted in two districts viz. Allahabad and Jaunpur of its native tracts in Uttar Pradesh. The overall average body weights of male goats of the age groups 0-3, 3-6, 6-9, 9-12 and above12 months were observed as 5.50±0.327, 12.07±0.598, 19.62±0.738, 21.92±0.571 and 30.44±0.978 kg respectively. The overall average body length of male goats of the age groups 0-3, 3-6, 6-9, 9-12, and above 12 months were observed as 38.63±1.762, 48.55±2.090, 58.77±0.652, 67.23±0.777 and 67.56±0.747 cm. The overall average height of male goats of the age groups 0-3, 3-6, 6-9, 9-12 and above 12 months were observed as 41.63±1.945, 53.36±2.094, 66.23±1.051, 72.69±0.664 and 72.31±0.893 cm, respectively. The corresponding values for females were 44.24±0.825, 50.38±0.836, 62.81±0.466, 69.14±0.387 and 69.00±0.691 cm. The mean value of horn size of male goats of the age groups 0-3, 3-6, 6-9, 9-12 and above 12 months were observed as 1.94±0.346, 2.73±0.449, 5.54±0.584,5.85±0.696 and 7.56±0.381 cm respectively. The respective averages for females were 1.77±0.171, 2.60±0.173, 4.94±0.322, 6.53±0.424 and 6.79±0.421 cm. The results revealed no significant differences in average slaughter weight between the sexes of the same age groups but it varied significantly (P less than 0.05) in different age groups like 6, 9 and 12 month of age.

Bio-Ecological Research of Lily Leaf Beetle Lilioceris lilii Scopoli (Coleoptera: Chrysomelidae) in Bosnia and Herzegovina

In the present study some bio-ecological characteristics of Lilioceris lilii in Bosnia and Herzegovina are presented. The insect’s presence was confirmed in the majority of the researched locations in Bosnia and Herzegovina. The average body length of L. lilii was 7.64 mm (7.025 mm for the males while 8.106 mm for the females) and the average body width 3.75 mm. The average length of a fully developed larva was 6.36 mm, and the average width was 2.69 mm. In terms of the cocoon, the average length was 8.83 mm and its width was 5.82 mm. Duration of embryonic development under field conditions was 8.5 days in average. The average duration of larval development (4 instars) was 16 days and the pupal stage lasted approximately 18 days. Lilioceris lilii retreated into winter quiescence at the beginning of autumn, thereof completes one generation per year. In the area of Sarajevo in field conditions the number of the laid eggs per female was 130 in average. The insect undergoes its entire development at the Asian and Oriental species of lily, on all the above-ground parts of the plants. Lilium candidum lily species that was present in all home gardens in the observed areas was the major host of the L. lilii. Experimentation with Aloae vera proved that this species was not a host of L. lilii.

Tingkat keragaman ukuran benih ikan lele Clarias sp. yang diberi Artemia dengan periode yang berbeda

<p class="NoParagraphStyle"><strong>ABSTRACT</strong></p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle">This research was conducted to evaluate the effect of different <em>Artemia</em> feeding period on the size variation of catfish <em>Clarias</em> sp. larvae. One thousand two days post hatched larvae with an average body length of 0.7±0.03 cm were randomly distributed into 25 L round plastic tanks. The treatment conducted for 13 days with four treatment; without<em> Artemia</em>, given <em>Artemia</em> 1, 2, or 3 days in the first culture period. The results showed that 15 days old fish has the same survival rate in all treatments; and has two size distribution i.e. the small size (S) and medium size (M). Increasingly the period of administration of <em>Artemia</em>, the percentage of the amount of M-size fish increases, thereby giving <em>Artemia</em> were able to increase growth of larvae. Feeding catfish with <em>Artemia</em> for two days has shown producing better growth.</p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle">Keywords: catfish, <em>Artemia</em>, size variation, growth performance</p><p class="NoParagraphStyle"><strong> </strong></p><p class="NoParagraphStyle"><strong> </strong></p><p class="NoParagraphStyle"><strong>ABSTRAK</strong></p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle">Penelitian ini dilakukan untuk mengevaluasi pengaruh perbedaan periode pemberian pakan <em>Artemia </em>terhadap kinerja pertumbuhan larva ikan lele <em>Clarias </em>sp. Seribu ekor larva lele yang berumur dua hari dengan panjang rata-rata 0,7±0,03 cm ditebar dalam wadah dengan volume air 25 L. Budidaya ikan dilakukan selama 13 hari dengan empat perlakuan, yaitu perlakuan tanpa pemberian <em>Artemia</em>, pemberian <em>Artemia</em> selama satu, dua, atau tiga hari di awal masa budidaya. Hasil penelitian menunjukkan bahwa ikan umur 15 hari memiliki sintasan yang sama di semua perlakuan, serta memiliki dua sebaran ukuran, yakni ukuran kecil (S) dan ukuran sedang (M). Semakin lama periode pemberian <em>Artemia</em>, persentase jumlah ikan ukuran M meningkat, sehingga pemberian <em>Artemia</em> pada larva ikan lele mampu meningkatkan pertumbuhan. Pemberian <em>Artemia</em> selama dua hari memberikan pertumbuhan yang lebih baik.</p><p class="NoParagraphStyle"> </p><p>Kata kunci: ikan lele, <em>Artemia</em>, keragaman ukuran, kinerja pertumbuhan</p>