Antimicrobial Activity of Skin Secretion of Rana hosii Frog (Anura: Ranidae) against several pathogenic microbes

The research on antimicrobial activity of skin secretion derived from Rana hosii (Anura: Ranidae) against several pathogenic microbes had been conducted at Microbiology Laboratory and at Genetics and Biomolecular Laboratory, both in the Biology Department, Universitas Andalas. The research used to survey and experimental methods. The study aimed to determine the effectivity of Rana hosii skin secretion to inhibit microbial activity, hence it tested onto some pathogenic microbes such as Escherichia coli, Staphylococcus aureus and Candida albicans. The results showed that the biggest inhibition zone formed against E. coli was 10.96 + 0.63 mm, on S. aureus was 11.74 + 1.00 mm, while on C. albicans was 7.42 + 0.80 mm. The results indicated that the skin secretions from Rana hosii frog could be a potential broad-spectrum antibacterial, and antifungal activity against C. albicans.

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Synthesis and Antimicrobial Screening of Some Novel 2, 5-Disubstituted 1, 3, 4-oxadiazole Derivatives

E-Journal of Chemistry ◽

10.1155/2011/905643 ◽

2011 ◽

Vol 8 (s1) ◽

pp. S149-S154 ◽

Cited By ~ 2

Author(s):

P. Panneerselvam ◽

G. Geete Ganesh

Keyword(s):

Antimicrobial Activity ◽

Antifungal Activity ◽

Antibacterial And Antifungal Activity ◽

Mass Spectral Data ◽

Mass Spectral ◽

E Coli ◽

H Nmr ◽

Oxadiazole Derivatives ◽

Antibacterial And Antifungal ◽

New Compounds

The syntheses of series of 2, 5-disubstituted 1, 3, 4-oxadiazole derivatives are described. A total of twelve new compounds were synthesized and characterized by IR,1H-NMR and Mass spectral data. All newly synthesized compounds were screened for their antimicrobial activityi.e. antibacterial activity againstS. aureusandE. coliand antifungal activity against fungusA. nigar. CompoundsG5andG7exhibited significant both antibacterial and antifungal activity whileG2,G10andG3,G9showed antibacterial and antifungal activity respectively. These compounds were 2, 5-disubstituted 1, 3, 4-oxadiazole moiety at position two and five showed reasonable antibacterial and antifungal activity.

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Substituted 4-Formyl-2H-chromen-2-ones: Their Reaction with N-(2,3,4,6-Tetra-Oacetyl- β-D-galactopyranosyl)thiosemicarbazide, Antibacterial and Antifungal Activity of Their Thiosemicarbazone Products

Current Organic Chemistry ◽

10.2174/1385272824999200812132256 ◽

2020 ◽

Vol 24 (19) ◽

pp. 2272-2282

Author(s):

Vu Ngoc Toan ◽

Nguyen Minh Tri ◽

Nguyen Dinh Thanh

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Candida Albicans ◽

Selenium Dioxide ◽

Antibacterial And Antifungal Activity ◽

E Coli ◽

Antibacterial And Antifungal Activities ◽

Alkyl Ethers ◽

Antibacterial And Antifungal

Several 6- and 7-alkoxy-2-oxo-2H-chromene-4-carbaldehydes were prepared from corresponding alkyl ethers of 6- and 7-hydroxy-4-methyl-2-oxo-2H-chromen-2-ones by oxidation using selenium dioxide. 6- and 7-Alkoxy-4-methyl-2H-chromenes were obtained with yields of 57-85%. Corresponding 4-carbaldehyde derivatives were prepared with yields of 41-67%. Thiosemicarbazones of these aldehydes with D-galactose moiety were synthesized by reaction of these aldehydes with N-(2,3,4,6-tetra-O-acetyl-β-Dgalactopyranosyl) thiosemicarbazide with yields of 62-74%. These thiosemicarbazones were screened for their antibacterial and antifungal activities in vitro against bacteria, such as Staphylococcus aureus, Escherichia coli, and fungi, such as Aspergillus niger, Candida albicans. Several compounds exhibited strong inhibitory activity with MIC values of 0.78- 1.56 μM, including 8a (against S. aureus, E. coli, and C. albicans), 8d (against E. coli and A. niger), 9a (against S. aureus), and 9c (against S. aureus and C. albicans).

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Substituted 6,7-dimethoxy-5-oxo-2,3,5,9b-tetrahydrothiazolo[2,3-a]isoindole- 3-1,1-dioxide Derivatives with Antimicrobial Activity and Docking Assisted Prediction of the Mechanism of their Antibacterial and Antifungal Properties

Current Topics in Medicinal Chemistry ◽

10.2174/1568026620666200922114735 ◽

2020 ◽

Vol 20 (29) ◽

pp. 2681-2691

Author(s):

Athina Geronikaki ◽

Victor Kartsev ◽

Phaedra Eleftheriou ◽

Anthi Petrou ◽

Jasmina Glamočlija ◽

...

Keyword(s):

Antimicrobial Activity ◽

Antifungal Activity ◽

Drug Targets ◽

Bacterial Species ◽

Pharmaceutical Research ◽

Docking Studies ◽

Fungal Species ◽

Docking Analysis ◽

E Coli ◽

Antibacterial And Antifungal

Background: Although a great number of the targets of antimicrobial therapy have been achieved, it remains among the first fields of pharmaceutical research, mainly because of the development of resistant strains. Docking analysis may be an important tool in the research for the development of more effective agents against specific drug targets or multi-target agents 1-3. Methods: In the present study, based on docking analysis, ten tetrahydrothiazolo[2,3-a]isoindole derivatives were chosen for the evaluation of the antimicrobial activity. Results: All compounds showed antibacterial activity against eight Gram-positive and Gram-negative bacterial species being, in some cases, more potent than ampicillin and streptomycin against all species. The most sensitive bacteria appeared to be S. aureus and En. Cloacae, while M. flavus, E. coli and P. aeruginosa were the most resistant ones. The compounds were also tested for their antifungal activity against eight fungal species. All compounds exhibited good antifungal activity better than reference drugs bifonazole (1.4 – 41 folds) and ketoconazole (1.1 – 406 folds) against all fungal species. In order to elucidate the mechanism of action, docking studies on different antimicrobial targets were performed. Conclusion: According to docking analysis, the antifungal activity can be explained by the inhibition of the CYP51 enzyme for most compounds with a better correlation of the results obtained for the P.v.c. strain (linear regression between estimated binding Energy and log(1/MIC) with R 2 =0.867 and p=0.000091 or R 2 = 0.924, p= 0.000036, when compound 3 is excluded.

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Antibacterial activity of ginger extracts on bacteria isolated from digestive tract infection patients attended Muhoza Health Center

Asian Journal of Medical Sciences ◽

10.3126/ajms.v11i2.27449 ◽

2020 ◽

Vol 11 (2) ◽

pp. 35-41

Author(s):

Callixte Yadufashije ◽

Adolyne Niyonkuru ◽

Emanuel Munyeshyaka ◽

Sibomana Madjidi ◽

Joseph Mucumbitsi

Keyword(s):

Antimicrobial Activity ◽

Antibacterial Activity ◽

Tract Infection ◽

Health Center ◽

Digestive Tract ◽

Inhibition Zone ◽

Ginger Extract ◽

E Coli ◽

Shigella Spp ◽

Stool Samples

Background: Ginger (Zingiber officinale) has been used for long time due to its potential antimicrobial activity against diversity of microbial pathogens. Aims and Objectives: The study was carried out to investigate the bacteria pathogens found in digestive tract infections and assess antimicrobial activities of ginger extract to identified bacteria. Materials and Methods: Bacteriological studies were carried out on stool samples from 30 patients attending Muhoza health center. Different types of bacteria were isolated from stool samples of digestive tract infection patients by using various methods such culture, biochemical test and antimicrobial activity of ginger extracts was analyzed at INES-Ruhengeri in clinical microbiology laboratory. Results: Study findings showed isolated bacteria and antibacterial activity of Ginger. Isolated bacteria and their percentages including Escherichia coli (46.6%) which is the predominant isolated bacteria, Salmonella species (33.33%), Enterobacter spp (10.0%), Shigella spp (6.6%) and Citrobacter (3.33%) which is the least isolated bacteria. Antibacterial activity of ginger was seen on isolated bacteria, as ethanol and methanol were used for ginger oil extraction, the antibacterial activity of ginger extracts using ethanol was seen on isolated bacteria such us Citrobacter spp with 14 mm of inhibition zone, Shigella spp with 12 mm, Salmonella with 11.1 mm, E. coli with 9.5 mm and Enterobacter spp which was seen to be resistant to ginger extract using ethanol with 0.66mm of inhibition zone. For methanol extracts antibacterial activity was seen as follows: Citrobacter spp at 12 mm, Shigella spp at 11 mm, E. coli at 8 mm, salmonella spp at 6.1 mm, and Enterobacter spp with 5 mm. Enterobacter spp was seen to be the most resistant bacteria in both extracts. Conclusion: Ginger has shown to have an antibacterial activity on bacteria isolated from digestive tract infected patients. It can be used as a medicine to treat these infections. Number of researches should be done to be sure on this reality of antibacterial activity of ginger.

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Antibacterial and Antifungal Activity of Clove Extract (Syzygium Aromaticum): Review

Eureka Herba Indonesia ◽

10.37275/ehi.v2i2.18 ◽

2021 ◽

Vol 2 (2) ◽

pp. 93-103

Author(s):

Nathaniel Hiwandika ◽

Susana Elya Sudrajat ◽

Ika Rahayu

Keyword(s):

Antifungal Activity ◽

Antifungal Agents ◽

Ethanolic Extract ◽

Therapeutic Effects ◽

Syzygium Aromaticum ◽

Antibacterial And Antifungal Activity ◽

E Coli ◽

Clove Essential Oil ◽

Clove Extract ◽

Antibacterial And Antifungal

Cloves is one of the native Indonesian plants and is used in many aspects of life. Cloves are used in multiple industries and as detergents, soaps, perfumes, food seasonings, aromatherapy, etc. Cloves are mainly used for ingredients in kretek cigarettes in Indonesia. Various studies of cloves reported they have good pharmacological and therapeutic effects. The main compounds of clove extract are eugenol and β-caryophyllene, which are powerful antibacterial and antifungal agents. The clove ethanolic extract showed the activity to inhibit Gram positive and negative bacteria such as B. cereus, S. aureus, E. coli, P. aeruginosa, S. pneumoniae, S. aureus, S. epidermidis, A. hydrophila, K. pneumoniae, P. gingivalis, and P. mirabilis. Clove essential oil has shown the ability to inhibit the growth of V. inaequalis, C. albicans, C. glabrata, and C. tropicalis. Cloves extract can be used as an essential ingredient of various medicines. However, it requires further research and trials.

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SYNTHESIS, CHARACTERIZATION AND ANTIMICROBIAL ACTIVITY OF NOVEL PYRAZOLO[3,4-b]PYRIDINES AND THEIR SPIRO-HETEROCYCLIC DERIVATIVES

JOURNAL OF ADVANCES IN CHEMISTRY ◽

10.24297/jac.v10i7.6802 ◽

2014 ◽

Vol 10 (7) ◽

pp. 2901-2915 ◽

Cited By ~ 4

Author(s):

Shawkat A. Abdelmohsen ◽

Talaat I El Emary

Keyword(s):

Antimicrobial Activity ◽

Aromatic Aldehydes ◽

Antibacterial And Antifungal Activity ◽

Pyrimidine Derivatives ◽

Spiro Derivatives ◽

Heterocyclic Derivatives ◽

Bacteria And Fungi ◽

Antibacterial And Antifungal ◽

New Compounds ◽

Hydrazone Derivative

The present work describes the synthesis of a novel series of heterocyclic moieties derived from 5-acetylpyrazolo[3,4-b]pyridine (1). The formation of chalcones (2a-d) was utilized to synthesize pyrazoline, isoxazoline and pyrimidine derivatives (3-10). Thiosemicarbazone and semicarbazone (11, 17) were utilized to synthesize other new triazolethiones, thiadiazole and selenadiazole derivatives (11-19). Some new spiro derivatives (22-25) were synthesized by the reaction of chalcone (21) of 1 and isatine with hydrazines, hydroxyl amines and thiourea. Also, The reaction of 1 with cyanoacetyl hydrazine gave the hydrazide-hydrazone derivative 26, which was allowed to react with aromatic aldehydes and α-cyanocinnamonitrile to afford coumarine and substituted pyridine derivatives (28, 29). The structures of all the new compounds have been established on the basis of their analytical and spectral data. Twenty two of the synthesized compounds were also evaluated for their antibacterial and antifungal activity against various strains of bacteria and fungi and most are found to possess promising antimicrobial activity when compared with Chloramphenicol and Clotrimazole

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Synthesis , spectroscopic characterization and biological study of some new Schiff bases based on 2-hydroxybenzadehyde

JOURNAL OF ADVANCES IN CHEMISTRY ◽

10.24297/jac.v13i12.6117 ◽

2017 ◽

Vol 13 (2) ◽

pp. 5995-6005

Author(s):

S. Abdelnabi ◽

Chasib K. Bkhakh ◽

Mouayed Y. Kadhum

Keyword(s):

Antimicrobial Activity ◽

Antifungal Activity ◽

Schiff Bases ◽

Aromatic Amines ◽

Spectroscopic Characterization ◽

Biological Study ◽

Antibacterial And Antifungal Activity ◽

Element Analysis ◽

Antibacterial And Antifungal

Four new Schiff bases were synthesized from condensation of aldehyde ( 2-hydroxy benzaldehyde ) with aromatic amines (4-introaniline ; 4,4-oxydianiline ; 4,4-diaminodipleny sulfone and sulfanilamide) . These Schiff bases were characterized by IR, NMR , GC. mass and element analysis. Moreover, the Schiff bases were screened for their antibacterial and antifungal activity against various microorganisms and compared with standard compounds. In general , the results were indicated that some Schiff bases had good antimicrobial activity.

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Antibacterial and Antifungal Activity of the Human Endometrial Fluid during the Natural Cycle

Infectious Diseases in Obstetrics and Gynecology ◽

10.1155/2021/8849664 ◽

2021 ◽

Vol 2021 ◽

pp. 1-9

Author(s):

Marta Bregón-Villahoz ◽

Maria-Dolores Moragues ◽

Inés Arrieta-Aguirre ◽

Mikel Azkargorta ◽

Lucía Lainz ◽

...

Keyword(s):

Antimicrobial Activity ◽

Antibacterial Activity ◽

Activity Patterns ◽

Antimicrobial Effect ◽

The Other ◽

Natural Cycle ◽

E Coli ◽

Antibacterial And Antifungal ◽

Related Pattern ◽

Embryo Transfer Catheter

Purpose. Some microbiota patterns have been associated with favorable IVF prognosis and others with pathological conditions. The endometrial fluid aspirate (EFA) contains antibacterial proteins that are enriched in implantative IVF cycles, but the antimicrobial effect of EFA has not been addressed. We aimed to evaluate the antimicrobial activity of the human endometrial fluid during the natural cycle. Methods. EFA was obtained through an embryo transfer catheter in 38 women, aged 18-40 years, with regular cycles attending to a fertility clinic. The antimicrobial activity of EFAs was tested against two strains of Staphylococcus aureus; one strain each of Streptococcus agalactiae, Enterococcus faecalis, Escherichia coli, and Klebsiella pneumoniae; and three yeasts (Candida albicans, Candida glabrata, and Candida krusei). Results. All samples exhibited antibacterial activity against S. aureus. In addition, 32.4% of EFAs were active against one of the other microorganisms assayed, 16.2% against two, and 5.4% against four of them. In contrast, none exhibited antibacterial activity against E. coli or K. pneumoniae. The antimicrobial activity differs considerably between EFA samples, and we failed to observe a cycle-related pattern. Conclusions. EFA presented two antimicrobial activity patterns: (a) one common to all the samples, exhibiting activity against S. aureus and lack of activity against E. coli and K. pneumoniae, and (b) an individualized pattern, showing activity against some of the other microorganisms tested. The intensity of antibacterial activity differs between EFA samples. Our data suggest that the uterine microbiota is controlled by means of endometrial fluid components.

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ISOLASI MIKROBA ENDOFITIK DARI TANAMAN OBAT SAMBUNG NYAWA Gynura procumbens) DAN ANALISIS POTENSINYA SEBAGAI ANTIMIKROBA

Journal of Biological Researches ◽

10.23869/bphjbr.13.1.200714 ◽

2007 ◽

Vol 13 (1) ◽

pp. 85-90 ◽

Cited By ~ 2

Author(s):

Rumella Simarmata ◽

Sylvia Lekatompessy ◽

Harmastini Sukiman

Keyword(s):

Antimicrobial Activity ◽

Endophytic Fungi ◽

Endophytic Bacteria ◽

Antimicrobial Agents ◽

Inhibition Zone ◽

Pseudomonas Sp ◽

E Coli ◽

Beneficial Effects ◽

Medical Plants ◽

Promising Source

Sambung nyawa (Gynura procumbens) has many beneficial effects to human health, such as decreasing blood pressure, maintaining blood sugar level (hypoglycaemic), decreasing cholesterol, a remedy for kidney trouble, antibacterial and lessen the inflamation (antiinflamation). This research was undertaken to discover the potency of endophytic microbes from sambung nyawa as antimicrobial agents. The purpose of this research was to screen the endophytic bacteria and the endophytic fungi having antimicrobial activity, which were isolated from stems, leaves, roots and fruits of medical plants, sambung nyawa. The antimicrobial activity was determined by measuring the growth inhibition of pathogenic microbes i.e Candida albicans, Escherichia coli, Pseudomonas sp. and Bacillus subtilis. A total of 38 isolates of bacteria and 15 isolates of fungi were obtained from sambung nyawa. Analysis demonstrated that, 45 percent isolates of bacteria and 20 percent isolates of fungi exhibited inhibitory activity. Antimicrobial activity was found in 21 percent of the isolates that inhibited the growth of C. albicans, E. coli, Pseudomonas sp., and B. subtilis, whereas 24 percent of isolates had activity only against B. subtilis. Isolate of endophytic bacteria USN 1.1 and USN 2.3 showed the most significant of inhibition zone. The Inhibition zone of the isolate USN 1.1 to C. albicans, E. coli, Pseudomonas sp, and B. subtilis were 2.318 cm2, 0.969 cm2, 0.796 cm2, and 0.381 cm2, respectively. The Inhibition zone of the isolate USN 2.3 to C. albicans, E. coli, Pseudomonas sp., and B. subtilis were 3.01 cm2, 0.519 cm2, 0.588 cm2 and 0.83 cm2, respectively. These results indicated that endophytic bacteria and endophytic fungi could be a promising source for antimicrobial agents.

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Lichen Xanthones as Models for New Antifungal Agents

Molecules ◽

10.3390/molecules23102617 ◽

2018 ◽

Vol 23 (10) ◽

pp. 2617 ◽

Cited By ~ 9

Author(s):

Diana Resende ◽

Patrícia Pereira-Terra ◽

Ângela Inácio ◽

Paulo Costa ◽

Eugénia Pinto ◽

...

Keyword(s):

Antimicrobial Activity ◽

Antibacterial Activity ◽

Fungicidal Activity ◽

Antifungal Agents ◽

Multidrug Resistant ◽

Antimicrobial Compounds ◽

Pathogenic Microorganisms ◽

Antibacterial And Antifungal Activity ◽

Chemistry Research ◽

Antibacterial And Antifungal

Due to the emergence of multidrug-resistant pathogenic microorganisms, the search for new antimicrobial compounds plays an important role in current medicinal chemistry research. Inspired by lichen antimicrobial xanthones, a series of novel chlorinated xanthones was prepared using five chlorination methods (Methods A–E) to obtain different patterns of substitution in the xanthone scaffold. All the synthesized compounds were evaluated for their antimicrobial activity. Among them, 3-chloro-4,6-dimethoxy-1-methyl-9H-xanthen-9-one 15 showed promising antibacterial activity against E. faecalis (ATCC 29212 and 29213) and S. aureus ATCC 29213. 2,7-Dichloro-3,4,6-trimethoxy-1-methyl-9H-xanthen-9-one 18 revealed a potent fungistatic and fungicidal activity against dermatophytes clinical strains (T. rubrum, M. canis, and E. floccosum (MIC = 4–8 µg/mL)). Moreover, when evaluated for its synergistic effect for T. rubrum, compound 18 exhibited synergy with fluconazole (ΣFIC = 0.289). These results disclosed new hit xanthones for both antibacterial and antifungal activity.

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