Chemical characterization and anti‐breast cancer effects of silver nanoparticles using Phoenix dactylifera seed ethanolic extract on 7,12‐Dimethylbenz[a] anthracene‐induced mammary gland carcinogenesis in Sprague Dawley male rats

2019 ◽  
Vol 34 (1) ◽  
Author(s):  
Ghobad Mohammadi ◽  
Mohammad Mahdi Zangeneh ◽  
Akram Zangeneh ◽  
Zahra Minoosh Siavosh Haghighi
Keyword(s):  
Breast Cancer ◽  
Silver Nanoparticles ◽  
Mammary Gland ◽  
Chemical Characterization ◽  
Ethanolic Extract ◽  
Phoenix Dactylifera ◽  
Male Rats ◽  
Sprague Dawley

scholarly journals Brain Cortical and Hippocampal Dopamine: A New Mechanistic Approach for Eurycoma longifolia Well-Known Aphrodisiac Activity and Its Chemical Characterization

2019 ◽  
Vol 2019 ◽  
pp. 1-13 ◽  
Author(s):  
Shahira M. Ezzat ◽  
Marwa I. Ezzat ◽  
Mona M. Okba ◽  
Salah M. Hassan ◽  
Amgad I. Alkorashy ◽  
...  
Keyword(s):  
Chemical Characterization ◽  
Serum Testosterone ◽  
Total Body Weight ◽  
Male Rats ◽  
Sprague Dawley ◽  
Noradrenaline Content ◽  
Eurycoma Longifolia ◽  
Mechanistic Approach ◽  
Dopamine Content ◽  
Total Body

Eurycoma longifolia Jack (Fam.: Simaroubaceae), known as Tongkat Ali (TA), has been known as a symbol of virility and sexual power for men. Metabolic profiling of the aqueous extract of E. longifolia (AEEL) using UPLC-MS/MS in both positive and negative modes allowed the identification of seventeen metabolites. The identified compounds were classified into four groups: quassinoids, alkaloids, triterpenes, and biphenylneolignans. AEEL is considered safe with oral LD50 cut-off >5000 mg/kg. Oral administration of 50, 100, 200, 400, or 800 mg/kg of AEEL for 10 consecutive days to Sprague-Dawley male rats caused significant reductions in mounting, intromission, and ejaculation latencies and increased penile erection index. AEEL increased total body weight and relative weights of seminal vesicles and prostate. Total and free serum testosterone and brain cortical and hippocampal dopamine content was significantly elevated in treated groups with no significant effects on serotonin or noradrenaline content.

scholarly journals Pilot Study: Nutritional and Preclinical Safety Investigation of Fermented Hispidin-Enriched Sanghuangporus sanghuang Mycelia: A Promising Functional Food Material to Improve Sleep

2022 ◽  
Vol 8 ◽  
Author(s):  
I-Chen Li ◽  
Fang-Chia Chang ◽  
Ching-Chuan Kuo ◽  
Hsin-Tung Chu ◽  
Tsung-Ju Li ◽  
...  
Keyword(s):  
Pilot Study ◽  
Rem Sleep ◽  
Functional Food ◽  
Ethanolic Extract ◽  
Male Rats ◽  
Feed Consumption ◽  
Sprague Dawley ◽  
Food Material ◽  
Nrf2 Signaling Pathway ◽  
Preclinical Safety

Sleep disturbances have been the hallmark of the recent coronavirus disease 2019 pandemic. Studies have shown that once sleep is disrupted, it can lead to psychological and physical health issues which can, in turn, disrupt circadian rhythm and induce further sleep disruption. As consumers are trying to establish healthy routines, nutritional and preclinical safety investigation of fermented hispidin-enriched Sanghuangporus sanghuang mycelia (GKSS) as a novel food material for spontaneous sleep in Sprague-Dawley rats is conducted for the first time. Results showed that the nutritional analysis of GKSS including moisture, ash, crude lipid, crude protein, carbohydrate, and energy were found to be 2.4 ± 0.3%, 8.0 ± 2.5%, 1.7 ± 0.3%, 22.9 ± 1.2%, 65.1 ± 3.1%, and 367.1 ± 10.2 kcal/100 g respectively. In the 28-day repeated-dose oral toxicity study, only Sprague-Dawley male rats receiving 5 g/kg showed a slight decrease in feed consumption at week 3, but no associated clinical signs of toxicity or significant weight loss were observed. Although a significant reduction of the platelet count was found in mid- and high-dose GKSS treated male groups, such changes were noted to be within the normal range and were not correlated with relative spleen weight changes. Hence, the no observed adverse effect level (NOAEL) of GKSS was identified to be higher than 5 g/kg in rats. After the safety of GKSS is confirmed, the sleep-promoting effect of GKSS ethanolic extract enriched with hispidin was further assessed. Despite 75 mg/kg of GKSS ethanolic extract does not affect wakefulness, rapid eye movement (REM) sleep and non-REM (NREM) sleep, GKSS ethanolic extract at 150 mg/kg significantly decreased wakefulness and enhanced NREM and REM sleep. Interestingly, such effects seem to be mediated through anti-inflammatory activities via NF-E2-related factor-2 (Nrf2) signaling pathway. Taken together, these findings provide the preliminary evidence to studies support the claims suggesting that GKSS contained useful phytochemical hispidin could be considered as and is safe to use as a functional food agent or nutraceutical for relieving sleep problems mediated by Nrf2 pathway, which the results are useful for future clinical pilot study.

scholarly journals ETHANOLIC EXTRACTS OF HEDYOTIS CORYMBOSA L. IMPROVES MONOSODIUM IODOACETATE-INDUCE OSTEOARTHRITIS IN RAT

2017 ◽  
Vol 10 (3) ◽  
pp. 473
Author(s):  
Anton Bahtiar ◽  
Fitri Arum Sari ◽  
Mega Audina Putri ◽  
Natasha Linsie Corona Datunsolang ◽  
Ade Arsianti
Keyword(s):  
Immune System ◽  
Ethanolic Extract ◽  
Daily Practice ◽  
Negative Control Group ◽  
Negative Control ◽  
Male Rats ◽  
Knee Joints ◽  
Control Group ◽  
Sprague Dawley ◽  
Monosodium Iodoacetate

ABSTRACTObjective: The objective of this study is to explore the effects of the 70% ethanolic extract of pearl grass on the immune system of the osteoarthritismodel rat, characterized by the number of leukocytes and lymphocytes, and the histology of the joint. Osteoarthritis is a degenerative diseasecharacterized by chronic inflammation in the joints. Based on the daily practice of herbal medicine in some community in Indonesia, pearl grassusually used for anti-inflammation but not a lot of data to support it.Methods: We used 36 male rats Sprague-Dawley strain divided into 6 groups. Normal group was given 0.5% of CMC, the negative control group was given0.025 ml of sodium iodoacetate in 0.9% saline, the positive groups control group was given a suspension of glucosamine-chondroitin 135 mg/200 g bb,three were given pearls grass extract in various dose 5.625 mg, 11.25 mg, and 22.5 mg, respectively. 28 days after sodium iodoacetate induction, the extractswere given orally once daily for 21 days. Measurement of inflammation of knee joint and the number of leukocytes and lymphocytes were counted on day14th, 28th, and 49th after sodium iodoacetate induction. After treatment, all rats were sacrified and all knee joints were collected to subject for histology.Results: The results showed that the extract of pearl grass in all doses was able to decrease the number of leukocytes and lymphocytes significantlyand prevent proteoglycan degradation. The results showed that the extract of pearl grass with a given dose variations have antiinflammation effectand been able to protect proteoglycan significantly.Conclusion: Doses 3 (22.5 mg/200 g BW) is the best result. These results indicate that pearl grass can be further investigated as a treatment for osteoarthritis.Keyword: Osteoarthritis, Sodium iodoacetate, Pearl grass, Hedyotis corymbosa L. Lamk., Immune system. 

scholarly journals Ethanolic extract of Mimosa caesalpiniifolia leaves: Chemical characterization and cytotoxic effect on human breast cancer MCF-7 cell line

2014 ◽  
Vol 93 ◽  
pp. 64-69 ◽  
Author(s):  
M.J.D. Silva ◽  
A.J.S. Carvalho ◽  
C.Q. Rocha ◽  
W. Vilegas ◽  
M.A. Silva ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Line ◽  
Human Breast Cancer ◽  
Cytotoxic Effect ◽  
Chemical Characterization ◽  
Ethanolic Extract ◽  
Human Breast ◽  
Mcf 7

scholarly journals EFFECT OF 70% ETHANOL EXTRACT OF ORTHOSIPHONIS STAMINEUS BENTH LEAVES ON THE PHARMACOKINETIC PARAMETERS OF FUROSEMIDE IN MALE WHITE RATS

2017 ◽  
Vol 9 ◽  
pp. 54
Author(s):  
Riana Maya Oktaviani ◽  
Santi Purna Sari ◽  
Yahdiana Harahap
Keyword(s):  
White Male ◽  
Area Under The Curve ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Male Rats ◽  
Combination Group ◽  
Pharmacokinetic Parameters ◽  
Control Group ◽  
Sprague Dawley ◽  
White Rats

Objective: This study aimed to observe the effect of the 70% ethanol extract of Orthosiphonis stamineus Benth leaves on the pharmacokineticparameters of furosemide in white male rats.Methods: 18 Sprague–Dawley male rats were divided into three groups: The normal control group was given only 1% carboxymethyl cellulose,the furosemide group was given 7.2 mg/200 g body weight (BW) suspension of furosemide, and the combination group was given 700 mg/kg BWsuspension of the 70% ethanolic extract of O. stamineus Benth leaves for 4 days followed by a 7.2 mg/200 g BW suspension of furosemide. On the4th day of treatment, we performed orbital sinus blood sampling on the eyes of the rats and analyzed the levels of furosemide in plasma using highperformanceliquid chromatography.Results: Therefore, the results showed that the administration of the 70% ethanol extract of O. stamineus Benth leaves improves the pharmacokineticparameters of furosemide on Cpmax and the area under the curve (p<0.05).Conclusion: This study concludes that the 70% ethanol extract of O. stamineus Benth leaves improves the pharmacokinetic parameters of furosemidein white male rats.

scholarly journals Quercetin loaded folate targeted plasmonic silver nanoparticles for light activated chemo-photothermal therapy of DMBA induced breast cancer in Sprague Dawley rats

RSC Advances ◽  
2020 ◽  
Vol 10 (53) ◽  
pp. 31961-31978
Author(s):  
Pritha Bose ◽  
Amiya Priyam ◽  
Rajiv Kar ◽  
Shakti P. Pattanayak
Keyword(s):  
Breast Cancer ◽  
Silver Nanoparticles ◽  
Photothermal Therapy ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Simple Chemical ◽  
Chemical Technique

Novel plasmonic (>800 nm) pentagonal QRC-FA-AgNPs synthesized through simple chemical technique induces light activated combined chemo-photothermal efficacy against breast cancer.

scholarly journals Effect of Eleutherine americana Merr. bulb extract on blood pressure and heart rate in anesthetized hypertensive rats

2018 ◽  
Vol 5 (2) ◽  
pp. 119
Author(s):  
Yori Yuliandra ◽  
Rizki Oktarini ◽  
Armenia Armenia
Keyword(s):  
Blood Pressure ◽  
Heart Rate ◽  
Saline Solution ◽  
Ethanolic Extract ◽  
Male Rats ◽  
Antihypertensive Activity ◽  
Sprague Dawley ◽  
Blood Pressure Lowering Effect ◽  
Pressure Lowering ◽  
Eleutherine Americana

The effect of ethanolic extract of Eleutherine americana Merr. on the blood pressure and heart rate has been investigated. A number of 25 Sprague-Dawley male rats were induced with prednisone 1.5 mg/kg in combination with saline solution 2.5% for 14 days to obtain hypertension model. The rats were anesthetized and prepared for the recording of blood pressure and heart rate. The rats were divided into five groups receiving 100, 200, and 400 mg/kg of the extract, vehicle control, and captopril 30 mg/kg as the reference. The treatment was administered three times with 30 minutes interval. The systolic (SBP), diastolic (DBP), mean arterial pressure (MAP), and heart rate (HR) were recorded. The data were analyzed with two-way analysis of variance (ANOVA) followed by Duncan’s MRT (95% confidence interval). The study revealed that the extract exhibited a blood pressure lowering effect. The best antihypertensive activity of the extract was shown by the dose of 100 mg/kg. Meanwhile, HR was also affected inversely by the treatment, in which the lowest dose caused the highest increase. The study suggests that the extract of Eleutherine americana exhibits antihypertensive activity that may be potential for future development of drugs. 

scholarly journals Hepatoprotective Role of Ethanolic Extract ofVitex negundoin Thioacetamide-Induced Liver Fibrosis in Male Rats

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Farkaad A. Kadir ◽  
Normadiah M. Kassim ◽  
Mahmood A. Abdulla ◽  
Wageeh A. Yehye
Keyword(s):  
Body Weight ◽  
Liver Fibrosis ◽  
Histopathological Examination ◽  
Weight Ratio ◽  
Ethanolic Extract ◽  
Hepatoprotective Activity ◽  
Male Rats ◽  
High Dose ◽  
Sprague Dawley ◽  
Standard Drug

The hepatoprotective activity of ethanolic extract from the leaves ofVitex negundo(VN) was conducted against thioacetamide- (TAA-) induced hepatic injury inSprague Dawleyrats. The therapeutic effect of the extract was investigated on adult male rats. Rats were divided into seven groups: control, TAA, Silymarin (SY), and VN high dose and low dose groups. Rats were administered with VN extract at two different doses, 100 mg/kg and 300 mg/kg body weight. After 12 weeks, the rats administered with VN showed a significantly lower liver to body weight ratio. Their abnormal levels of biochemical parameters and liver malondialdehyde were restored closer to the normal levels and were comparable to the levels in animals treated with the standard drug, SY. Gross necropsy and histopathological examination further confirmed the results. Progression of liver fibrosis induced by TAA in rats was intervened by VN extract administration, and these effects were similar to those administered with SY. This is the first report on hepatoprotective effect of VN against TAA-induced liver fibrosis.

scholarly journals The Role of Oily and Methanolic Extracts of Phoenix Dactylifera Leaves in Ameliorating CCl4 Cytotoxicity in Male Rats

2020 ◽  
Vol 44 ((E0)) ◽  
pp. 88-93
Author(s):  
Saadia S.M. Al-Zeiny ◽  
Falah M.K. AL-Rekabi ◽  
Ahmed Sh. j. Al-rekabie ◽  
Duraid A. Abbas
Keyword(s):  
Oxidative Stress ◽  
Corn Oil ◽  
Methanolic Extract ◽  
Marrow Cell ◽  
Extended Period ◽  
Phoenix Dactylifera ◽  
Treated Group ◽  
Negative Control ◽  
Male Rats ◽  
Sprague Dawley

The study was performed to investigate the protective effect of different methanolic and oily extracts of leave and dry date of Phoenix dactylifera against oxidative stress induced by CCl4 on 49 Sprague-Dawley male rats weighed 175-200 g and aged 6-8 months. The animals were equally divided into 7 groups and assigned as follows: G1, administered 0.1 mL distilled water orally and considered control negative group (C-ve); G2, administered 0.1 mL/100 g BW corn oil (CrO); and G3 administered 100 mg/kg BW CCl4 orally for induction oxidative stress and considered control positive group (C+ve). The other four groups were initially administered 100 mg/kg BW CCl4 for oxidative stress induction and treated for two months as follows: G4, treated orally by 100 mg/kg BW of date methanolic extract (DME); G5, treated orally by 150 mg/kg BW of leaves methanolic extract (LME); G6, treated by 250 mg/kg BW date oily extract (DOE); while G7, treated by 250 mg/kg BW leaves oily extract (LOE). At the end of two months experiment, the animals were scarified, and their femurs removed for cytogenetic examination. results showed that only CCl4 group had significant increase (P< 0.05) increase 5.1±0.5 compared to negative control and all treated groups. CCl4 group also recorded clear increasing in percentage of chromosome aberrations including diverse types in bone marrow cell compared to rat groups treated by date and leaves methanolic and oily extracts and negative control groups. It could be concluded that the treatment with different palm date and leaves extracts failed to overcome the genotoxic effect of CCl4 completely. Possibly, because CCl4 dosed for extended period (2 months) might cause extensive cell and genetic damages could be opposed antioxidants presented in the different palm extracts recording some but lesser chromosomal aberration compared to that CCl4 treated group.

Ultrastructural Changes in the Mammary Epithelial Cell Population During Neoplastic Development Induced by A Chemical Carcinogen.

1976 ◽  
Vol 34 ◽  
pp. 250-251 ◽  
Author(s):  
J. Russo ◽  
W. Isenberg ◽  
M. Ireland ◽  
I.H. Russo
Keyword(s):  
Mammary Gland ◽  
Cell Population ◽  
Virgin Female ◽  
Histological Change ◽  
Mammary Epithelial ◽  
Female Rats ◽  
Ultrastructural Changes ◽  
Post Inoculation ◽  
Chemical Carcinogen ◽  
Sprague Dawley

The induction of rat mammary carcinoma by the chemical carcinogen DMBA is used as a model for the study of the human disease (1). We previously described the histochemical changes that occur in the mammary gland of DMBA treated animals before the earliest manifested histological change, the intraductal proliferation (IDP), was observed (2). In the present work, we demonstrate that a change in the stable cell population found in the resting mammary gland occurs after carcinogen administration.Fifty-five day old Sprague-Dawley virgin female rats were inoculated intragastrically with 20mg of 7,12-dimethylbenz(a)anthracene (DMBA) in 1ml sesame oil. Non-inoculated, age-matched females were used as controls. Mammary glands from control and inoculated rats were removed weekly from the time of inoculation until 60 days post-inoculation. For electron microscopy, the glands were immersed in Karnovsky's fixative, post-fixed in 1% OsO4, dehydrated, and embedded in an Epon-Araldite mixture. Thick (lμ) sections were stained with 1% toluidine blue and were used for selecting areas for ultrastructural study.

Sign in / Sign up

Export Citation Format

Share Document