Distribution and Antibiotics Resistance Pattern of Community-Acquired Methicillin-Resistance Staphylococcus aureus in Southwestern Nigeria

2021 ◽  
Author(s):  
Ibukunoluwa Olayinka Oginni ◽  
Ademola Adetayo Olayinka
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistance ◽  
Resistance Pattern ◽  
Antibiotics Resistance ◽  
Southwestern Nigeria

scholarly journals Study of Antibiotics Sensitivity Pattern And Molecular Characterization of Staphylococcus Aureus Isolated From Human And Animal Pyogenic Cases

2021 ◽  
Author(s):  
Jayshree Singh ◽  
Amit Kumar ◽  
Sharad K. Yadav ◽  
Ritika Yadav ◽  
Vinod K. Singh
Keyword(s):  
Staphylococcus Aureus ◽  
Molecular Characterization ◽  
Drug Sensitivity ◽  
Methicillin Resistance ◽  
Phenotypic Characterization ◽  
Resistance Pattern ◽  
Sensitivity Pattern ◽  
Drug Resistance Pattern ◽  
Animal Isolates

Abstract Staphylococcus aureus has been described as the most common cause of human and animal diseases and has emerged as superbug due to multidrug resistance. Considering these, a total of 175 samples were collected from pyogenic cases of humans (75) and animals (100), to establish the drug resistance pattern and also for molecular characterization of human and animal isolates. Thermonuclease (nuc) gene amplification was used to confirm all presumptive S. aureus isolates and then antibiotic sensitivity and slide coagulase tests were used for phenotypic characterization of isolates. Following that, all of the isolates were subjected to PCR amplification to detect the existence of the methicillin resistance (mecA) and coagulase (coa) genes. Lastly, typing was done by using the Randomly Amplified Polymorphic DNA-PCR. The overall prevalence of S. aureus in human and animal samples was found to be 39.4%. Drug sensitivity revealed the highest resistance against the β-lactam antibiotics such as ampicillin (94.8%) and penicillin (90.6%), followed by cephalosporin (cefixime-67.7%) and quinolone (ciprofloxacin-52.1%) group of drugs. The drug sensitivity was the highest against antibiotics like chloramphenicol (95%) followed by gentamicin (90%). Among the 69 S. aureus isolates, the overall presence of MRSA was 40.5% (27.5% and 50% in human and animal isolates respectively). Total 33 isolates exhibited coa genes amplification of more than one amplicons and variable in size of 250, 450, 800, and 1100 bp. The RAPD typing revealed amplification of 5 and 6 different band patterns in humans and animals, respectively, with two common patterns suggesting a common phylogenetic profile.

scholarly journals Multi-drug Resistance Pattern of Staphylococcus aureus from Paediatric Ward, General Hospital, Ikot-Ekpaw, Mkpat Enin LGA, Akwa-Ibom State, Nigeria

2019 ◽  
pp. 1-6
Author(s):  
I. A. Ibanga ◽  
O. D. Akan ◽  
F. Z. Uyanga ◽  
E. C. Mantu ◽  
M. Asuquo
Keyword(s):  
Staphylococcus Aureus ◽  
Drug Resistance ◽  
General Hospital ◽  
Skin Wound ◽  
Multi Drug Resistance ◽  
Resistance Pattern ◽  
Antibiotics Resistance ◽  
Paediatric Ward ◽  
Mannitol Salt Agar ◽  
Drug Resistance Pattern

Aim: This study evaluated the Multi-drug resistance (MDR) pattern of Staphylococcus aureus from a paediatric ward and was conducted using standard clinical microbiological procedures. Location and Duration of Study: The study was carried out on infant samples collected from the Paediatric ward, General Hospital, Ikot-Ekpaw,  Mkpat Enin LGA, Akwa-Ibom State, Nigeria, over three (3) months period. Design of Study: Hundred swab-samples were inoculated on Mannitol salt agar. Positive growths were further biochemically confirmed for Staphylococcus aureus. Confirmed isolates were then used for MDR evaluation. Results and Interpretation: Of the 100 samples from skin, wound, ear, throat and nose swabs, 28 isolates were confirmed as S. aureus and were subjected to a range of selected commercially available antibiotics like: Amoxicillin, ampiclox, chloramphenicol, ciprofloxacin, erythromycin, gentamicin, levofloxacin, norfloxacin, rifampicin and streptomycin, to evaluate their susceptibilities. The wound swabs gave the highest isolate percentage yield (32%) followed by skin swabs (29%). Susceptibility results showed that amoxicillin and ampiclox were more resisted by the isolates, while ciprofloxacin, levofloxacin and norfloxacin were more effective against the isolates. The Multiple antibiotics resistance (MAR) indices showed that 85.7% of the isolates had confirmed multi-drug resistance status, with 60.7% of the isolates showing resistance to between four or more of the tested antimicrobials. MAR indices revealed that 96.4% of the isolates had 0.3, indicating that the resistance resulted from isolates that adapted to the tested drugs due to some form of abuse. Restricted use of these drugs would help curtail the high resistance currently observed amongst microorganisms.

scholarly journals Antibiotics Resistance Pattern of Staphylococcus aureus Isolated From In-patients of an Orthopaedic Hospital in North-Western Nigeria

2021 ◽  
Vol 8 (4) ◽  
pp. 160-166
Author(s):  
Dominic Agbo Oche ◽  
Paul Ifeleke Oyegoke ◽  
Maryloveth Oluchukwu Akpudo ◽  
Busayo Olalekan Olayinka
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Bone Diseases ◽  
Diffusion Method ◽  
Resistance Pattern ◽  
Antibiotics Resistance ◽  
Drug Resistant ◽  
Disk Diffusion Method ◽  
Cross Sectional ◽  
Orthopaedic Hospital

Background and aims: Staphylococcus aureus, an important pathogen in bone diseases, is a highly multi-drug resistant (MDR) bacterium. This study aimed to investigate the antibiotic resistance among S. aureus isolated from patients on admission in an orthopaedic hospital. Methods: In this cross-sectional research, 140 samples comprising urine samples, wound swabs, and nasal swabs were collected from 49 patients on admission. Samples were cultured and screened for S. aureus following standard procedures. Using the agar-disk diffusion method, the isolates were subjected to antibiotics susceptibility tests. Results: S. aureus were isolated from 26 (18.6%) samples, and wound swabs were found to have the highest number of the S. aureus isolates with 12 (46.2%). Among the 26 S. aureus isolated, 25 (96.2%) isolates were resistant to at least four or more of the tested antibiotics. There were 23 (88.5%) MDR isolates, while there were only 2 (7.6%) extensively drug resistant ones. The number of methicillin-resistant S. aureus were 17 (65.4% of the isolates), while the number of methicillin-susceptible S. aureus were 9 (34.6% of the isolates). A total of 22 (84.6%) isolates had multi-antibiotic resistance (MAR) index greater than 0.2. Inducible clindamycin resistance of 2 (7.6%) was observed. Conclusion: This study showed that the S. aureus isolated from the patients were resistant to multiple antibiotics. Regular surveillance of antibiotic resistance is of utmost importance, since it facilitates the design or development of the treatment regimens that could check the spread of antimicrobial resistance.

scholarly journals Prevalence of methicillin resistance and superantigenic toxins in Staphylococcus aureus strains isolated from patients with cancer

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Effat Abbasi Montazeri ◽  
Azar Dokht Khosravi ◽  
Saeedeh Khazaei ◽  
Ali Sabbagh
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Cancer Patients ◽  
Methicillin Resistance ◽  
Resistance Pattern ◽  
Patients With Cancer ◽  
Resistance Patterns ◽  
Mrsa Strains ◽  
Southwest Iran ◽  
Antibiotic Resistance Patterns

Abstract Background This study aimed to determine the frequency of methicillin-resistant Staphylococcus aureus (MRSA), antibiotic resistance patterns, superantigenic toxins profile, and clonality of this pathogen in patients with cancer. Results In total, 79 (25.7%) isolates were confirmed as Staphylococcus species, from which 38 (48.1%) isolates were S. aureus, and 29 (76.3%) isolates were confirmed as MRSA. The highest resistance in MRSA strains was seen against ciprofloxacin (86.2%) and erythromycin (82.8%). Teicoplanin, and linezolid were the most effective antibiotics. From all MRSA isolates, 3 strains (10.3%) were resistant to vancomycin with minimum inhibitory concentration values of 128 μg/ml. The prevalence of superantigenic toxins genes was as follows: pvl (10.5%), tsst-1 (36.8%), etA (23.7%), and etB (23.7%). The t14870 spa type with frequency of 39.5% was the most prevalent clone type circulating in the cancer patients. Conclusions This study showed the circulating of spa t14870 as the most predominant MRSA clone in cancer patients of southwest Iran. Also, a diverse antibiotic resistance pattern and toxin profiles were seen among MRSA isolates.

scholarly journals Staphylococcus aureus and bovine mastitis: molecular typing of methicillinresistance and clinical description of infected quarters

2019 ◽  
Vol 70 (2) ◽  
pp. 1511
Author(s):  
Α. OREIBY ◽  
H. KHALIFA ◽  
A. EID ◽  
A. AHMED ◽  
T. SHIMAMOTO ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistance ◽  
Animal Health ◽  
Bovine Mastitis ◽  
Clinical Signs ◽  
Subclinical Mastitis ◽  
Clinical Severity ◽  
Resistance Pattern ◽  
Gram Positive ◽  
Staphylococcus Spp

This study targeted Staphylococcus aureus (S. aureus)-mastitis of bovine as a possible source of livestock-associated methicillin-resistant Staphylococcus aureus (MRSA), to describe clinical signs of mastitis associated with MRSA and methicillin-sensitive Staphylococcus aureus (MSSA). The study area was the Gharbia and Kafrelsheikh governorates, in the central and northern regions of the Egyptian Delta. Clinical examination of animals was performed to detect clinical mastitis (CM) and clinically normal cases were tested by the California mastitis test (CMT) to identify subclinical mastitis (SCM). Accordingly, 38 mastitis cases (68 infected quarters) were detected. Milk samples were collected aseptically and were cultured on Baird Parker agar. Fifty nine Gram-positive cocci-shaped isolates were selected and preserved. In addition, 33 Staphylococcus spp. isolates originated from bovine mastitis at the same study area were obtained from Animal Health Research Institute (AHRI). The cocci-shaped Gram-positive bacteria and AHRI Staphylococcus spp. isolates were used for molecular identification of S. aureus and MRSA. Molecular screening had yielded 17 S. aureus isolates, from which five isolates (29.41%) were MRSA and 12 isolates (70.59%) were MSSA. The five MRSA isolates were mecA positive, but mecC negative. Multilocus sequence typing (MLST) of the five MRSA isolates indicated that all were sequence type 1 (ST1). S. aureus-associated cases showed different clinical forms of mastitis, including subclinical, acute, chronic, and gangrenous. However, subclinical mastitis was the only detected form associated with MRSA, which may represent a potential hidden risk for humans. Phenotypic antimicrobial resistance pattern of MRSA isolates showed resistance to all of the tested β-lactam antimicrobials, with marked orgaresistance to tetracycline and gentamycin. Based on our knowledge, this is the first report to identify MRSA ST1 in Egypt. Bovine mastitis could be a source for the dissemination of MRSA to humans and other animals. Additionally,while methicillin-resistance may have no effect on the clinical severity of mastitis, it does affect therapeutic success, particularly when β-lactam antimicrobials are used.

scholarly journals Antibiotics Susceptibility Pattern of Bacteria Isolated from Ready-To-Eat Vegetables Sold in Mohammadu Gumi Market, Kaduna, Nigeria

2021 ◽  
Vol 16 (2) ◽  
pp. 131-137
Author(s):  
A.F. Obajuluwa ◽  
A. Kefas ◽  
S.K. Parom
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Susceptibility ◽  
Diffusion Method ◽  
Resistance Pattern ◽  
Resistant Bacteria ◽  
Antibiotics Resistance ◽  
Salmonella Spp ◽  
Methicillin Resistant ◽  
Agar Disc

Background: Freshly consumed vegetables are considered to have more nutritional value than cooked ones. However, they are a potential source of foodborne illnesses due to possible microbial contamination, this poses safety threat.Objectives: This study was carried out to determine the bacteriological quality of ready-to-eat vegetables sold in Mohammadu Gumi market, Kaduna and to determine the antibiotics resistance pattern of the bacteria isolates.Methods: A total of 40 samples of cabbage were collected. Gram staining, growth on selective media and biochemical tests were carried out to identify the isolates. Antimicrobial susceptibility testing was done using the Kirby-Bauer agar disc diffusion method. Methicillin resistant Staphylococcus aureus isolates were detected with the use of cefoxitin disc agar diffusion test.Results: A total of 46 bacteria isolates were obtained with a total colony count range from 4 – 9 x 106CFU/ml. The following bacteria were isolated:   aureus (41.3%), Escherichia coli (28.3%), Salmonella spp (19.6%),  Pseudomonas aeruginosa (8.7%) and Serratia spp (2.2%). The result of antimicrobial susceptibility test showed thatthe isolates were highly susceptible to ofloxacin: Staphylococcus aureus, Pseudomonas aeruginosa, Serratia spp (100%), E. coli (92.3%) and Salmonella spp (87.5%). The isolates were all resistant to penicillin and ampicillin while 89.5% of the Staphylococcus aureus isolates were phenotypically methicillin resistant Staphylococcus aureus.Conclusion: This study showed that the vegetables (cabbage) were highly contaminated with antibiotic resistant bacteria, this can be a source of infection to the consumers and a potential means of transmitting multidrug resistant bacteria strains in the community.

scholarly journals Different microbial and resistance patterns in primary total knee arthroplasty infections – a report on 283 patients from Lithuania and Sweden

2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Sujeesh Sebastian ◽  
Erdem Aras Sezgin ◽  
Justinas Stučinskas ◽  
Šarūnas Tarasevičius ◽  
Yang Liu ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Resistance ◽  
Knee Arthroplasty ◽  
Methicillin Resistance ◽  
National Level ◽  
Resistance Pattern ◽  
Microbial Profile ◽  
Primary Tkas ◽  
Drug Resistance Pattern ◽  
Total Knee

Abstract Background The microbiology and the susceptibility patterns of infected total knee arthroplasties (TKAs) vary depending on demographic, local antimicrobial stewardship, and surgical factors. We wanted to compare the recent microbial profile and antimicrobial resistance pattern in revisions due to infections after primary TKAs in Sweden and Lithuania. Our hypothesis was that there is a difference in bacteriology and resistance pattern based on patient related, societal and local hospital factors as almost similar praxis have been applied for TKA surgery, short term systemic prophylaxis and routine use of local gentamicin containing bone cement. Methods Primary TKAs revised for the first time due to verified or suspected infection were collected nationwide in Sweden during 2018, and in Lithuania between 2011 and 2020 from a single major TKA revision centre in Kaunas. We identified 202 TKAs in Sweden from the Swedish Knee Arthroplasty Register and 84 from Kaunas revised due to infection. We collected available culture reports and evaluated the type of microorganisms with antimicrobial resistance pattern at revision. Results The majority of the infected cases in Sweden were early-type prosthetic joint infection (PJI) (44%), whereas late-type PJI (52%) were more common in the Kaunas cases. Gram-positive bacteria prevailed in both Sweden (55%) and Lithuania (80%). Staphylococcus aureus was the most frequent organism identified in both countries (33% in Sweden and 34% in Lithuania). More polymicrobial infections were observed in Sweden than in Lithuania (16 and 6% respectively). Methicillin resistance in Staphylococcus aureus and coagulase-negative staphylococci were higher in Lithuania (4/28 and 19/29) than in Sweden (1/42 and 9/41). Conclusions The type of infections, microbial profile, and drug resistance pattern differed between Sweden and Lithuania. Societal and local hospitals factors with emerging resistance in Lithuania are the most plausible explanation for the difference. Lack of complete data on a national level in Lithuania underlines the importance of adding microbiology of PJIs in implant registers for national aggregation and allow cross country comparisons.

scholarly journals Antibiotic Sensitivity and Virulence Genes in Staphylococcus aureus Isolates from Surgical Site Infection

2021 ◽  
Vol 38 (1) ◽  
pp. 21-26
Author(s):  
Marufa Zerin Akhter ◽  
Nuheen Akter ◽  
Sunjukta Ahsan ◽  
Fatema Moni Chowdhury
Keyword(s):  
Staphylococcus Aureus ◽  
Surgical Site Infection ◽  
Virulence Genes ◽  
Methicillin Resistance ◽  
Meca Gene ◽  
Antibiotic Sensitivity ◽  
Virulence Gene ◽  
Resistance Pattern ◽  
Toxin Gene ◽  
Site Infection

Fourteen multi drug resistant Staphylococcus aureus isolates from surgical site infection were analyzed for their antibiotic sensitivity and the presence of nine virulence genes. The isolates showed a high resistance pattern, all being resistant to methicillin, oxacillin, azithromycin, ceftazidime and amoxyclav. Seven of the isolates were sensitive to linezolid; three were sensitive to trimethoprim: sulfamethoxazol and another three were sensitive to ciprofloxacin. Ceftriaxone, gentamicin and amikacin were the drugs of choice as nine (64.3%) isolates were sensitive to ceftriaxone, eleven (78.6%) were sensitive to gentamicin and another eleven (78.6%) to amikacin. The present study focused to identify nine important virulence genes including intrinsic methicillin resistance gene mecA, methicillin resistance assisting gene femA, toxic shock syndrome toxin gene tst, exfoliative toxin A and B genes, eta and etb, Panton Valentine leukocidin gene LukS/F-PVL, along with three enterotoxin genes sec, sed and see. According to the presence of mecA gene and antibiotic resistance profile, two isolates were identified as methicillin resistant Staphylococcus aureus. However, another isolate, despite harbouring both mecA and femA genes, was sensitive to ceftriaxone which excluded it from being considered as an MRSA. Thus, the ratio of MRSA can be considered to be quite high (2/14) among the strains. Interestingly, most of the isolates (10/14) harboured femA gene, the majority of which were mecA negative with an MSSA type antibiotic profile. Although considered as a marker for community acquired MRSA, LukS/F-PV was found in half of these nosocomial isolates. Five, four and two of the isolates harboured etb, tst and sec gene, respectively. However, all the isolates were negative for eta, sed and see genes. Two isolates showed the co-existance of “femA, LukS/F-PV, etb, and tst” genes. Another two virulence gene patterns observed were “femA, mecA, tst, sec” and “femA, LukS/F-PV, etb”. The presence of several virulence genes can be correlated to the highly pathogenic nature of the isolates. Bangladesh J Microbiol, Volume 38, Number 1, June 2021, pp 21-26

scholarly journals Eugenol Provokes ROS-Mediated Membrane Damage-Associated Antibacterial Activity against Clinically Isolated Multidrug-Resistant Staphylococcus aureus Strains

2016 ◽  
Vol 9 ◽  
pp. IDRT.S31741 ◽  
Author(s):  
Balaram Das ◽  
Debasis Mandal ◽  
Sandeep Kumar Dash ◽  
Sourav Chattopadhyay ◽  
Satyajit Tripathy ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Membrane Damage ◽  
Reactive Oxygen Species Generation ◽  
Multidrug Resistant ◽  
Vancomycin Resistance ◽  
Resistance Pattern ◽  
Antibiotics Resistance ◽  
Aureus Infection ◽  
Vancomycin Resistant

Due to the indiscriminate use of antibiotics, resistance to antibiotics has increased remarkably in Staphylococcus aureus. Vancomycin is the final drug to treat the S. aureus infection, but nowadays, resistance to this antibiotic is also increasing. So, the investigation of antibiotic resistance pattern is important. As there is already resistance to vancomycin, there is an urgent need to develop a new kind of antimicrobial to treat S. aureus infection. Eugenol may be the new drug of choice. This study was conducted to evaluate the antibacterial activity of eugenol against vancomycin-resistant S. aureus isolated from clinical pus samples. Thirty six pus samples were included in the study. Samples were isolated, identified and antimicrobial susceptibility tests were performed as per routine laboratory protocol. The antimicrobial activity and mechanisms of killing of eugenol were studied. Out of 36 pus samples, only 20 isolates were confirmed as S. aureus strains and 6 isolates exhibited vancomycin resistance. Eugenol successfully destroyed the vancomycin-resistant strains via reactive oxygen species generation and membrane damage. The prevalence of vancomycin resistance is increased day by day in different countries, and necessary steps to prevent the spread and emergence of resistance should be taken. The findings of the study suggested that eugenol might be used to treat vancomycin-resistant S. aureus.

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