scholarly journals 2-Methacryloyloxyethyl phosphorylcholine (MPC)-polymer suppresses an increase of oral bacteria: a single-blind, crossover clinical trial

2018 ◽  
Vol 23 (2) ◽  
pp. 739-746 ◽  
Author(s):  
Natsumi Fujiwara ◽  
Hiromichi Yumoto ◽  
Koji Miyamoto ◽  
Katsuhiko Hirota ◽  
Hiromi Nakae ◽  
...  

Abstract Objectives The biocompatible 2-methacryloyloxyethyl phosphorylcholine (MPC)-polymers, which mimic a biomembrane, reduce protein adsorption and bacterial adhesion and inhibit cell attachment. The aim of this study is to clarify whether MPC-polymer can suppress the bacterial adherence in oral cavity by a crossover design. We also investigated the number of Fusobacterium nucleatum, which is the key bacterium forming dental plaque, in clinical samples. Materials and methods This study was a randomized, placebo-controlled, single-blind, crossover study, with two treatment periods separated by a 2-week washout period. We conducted clinical trial with 20 healthy subjects to evaluate the effect of 5% MPC-polymer mouthwash after 5 h on oral microflora. PBS was used as a control. The bacterial number in the gargling sample before and after intervention was counted by an electronic bacterial counter and a culture method. DNA amounts of total bacteria and F. nucleatum were examined by q-PCR. Results The numbers of total bacteria and oral streptcocci after 5 h of 5% MPC-polymer treatment significantly decreased, compared to the control group. Moreover, the DNA amounts of total bacteria and F. nucleatum significantly decreased by 5% MPC-polymer mouthwash. Conclusions We suggest that MPC-polymer coating in the oral cavity may suppress the oral bacterial adherence. Clinical relevance MPC-polymer can be a potent compound for the control of oral microflora to prevent oral infection.

Author(s):  
Ali Mohammadpour ◽  
Mousa Sajadi ◽  
Somayyeh Maghami ◽  
Hossein Soltani

Objective: Increased gastric residual volume is a complication of enteral nutrition intolerance that leads to gastrointestinal complications such as nausea, vomiting, and aspiration pneumonia. The present study was conducted to determine the effect of gastric gas emptying on the residual gastric volume in mechanically-ventilated patients fed through nasogastric tubes.Methods: This randomized, single-blind, clinical trial was conducted on two groups of patients in the intensive care unit (ICU) of Kamyab Hospital of Mashhad. A total of 64 patients were randomly divided into a case and a control group. In the case group, the gastric gases accumulated through the nasogastric tube were emptied by applying palm pressure on the epigastric region. The control group did not undergo this intervention but received the routine care provided in the ward. Data were collected using a demographic questionnaire and a form containing records of the patients’ residual gastric volume and disease-related information. The residual gastric volume was measured and compared in the two groups before and after the intervention. Data were analyzed in SPSS-19 using the Chi-square test, the independent t-test, and the repeated measures ANOVA at the significance level of 5%.Results: The residual gastric volume did not differ significantly between the two groups before the intervention (p=0.14); after the intervention; however, a significant reduction was observed in the case group compared to the controls (p=0.007).Conclusion: Gastric gas emptying helps reduce the residual gastric volume in mechanically-ventilated patients fed through nasogastric tubes. Further studies are recommended to further ensure the benefits of this method.


2021 ◽  
Author(s):  
Linda LD Zhong ◽  
Chi-Fung CHOY ◽  
Hung-Wai CHO ◽  
Yi-Ping WONG ◽  
Alan Yat-Lun WONG ◽  
...  

Abstract Background: Olfactory dysfunction (OD) was recognized as one of common symptom of COVID-19. OD, defined as the reduced or distorted ability to smell during sniffing (orthonasal olfaction) and may represent one of early symptoms in the clinical course of COVID-19 infection. A large online questionnaire-based survey found that some of post COVID-19 patients showed no improvement at one month after they were discharged from hospital. Therefore, this clinical trial is designed to explore the efficacy of acupuncture for OD in infected COVID-19 patients and to determine whether acupuncture could have benefits than sham acupuncture for OD in post- COVID-19 patients.Methods: This is a single blind, randomized controlled, cross over trial. We plan to recruit forty post-COVID-19 patients who are presenting with smell loss or smell distortions more than one month. Qualified patients will be randomly allocated into the intervention group (real acupuncture) or the control group (sham acupuncture) in a 1:1 ratio. Each patient will receive 8 sessions of treatment over 4 week (Cycle 1), and 2-week follow-up. After the follow-up, the control group will be conducted with real acupuncture for another 4 weeks (Cycle 2), and the real acupuncture group will be conducted with the 4-week sham acupuncture. The primary outcomes are the scores change on the questionnaire of olfactory functioning and olfaction related quality of life at weeks 6, 8, 12 and 14 from the baseline. Secondary outcome is the change on the Olfactory Test score at the week 6 and 12 from the baseline measured by using the Traditional Chinese version of the University of Pennsylvania Smell Identification Test (UPSIT-TC).Discussion: The results of this trial will help to determine the effectiveness of acupuncture for OD in post-COVID-19 Patients. This may provide a new treatment option for patients.Trial registration: ClinicalTrials.gov, NCT04959747, Registered on July 13, 2021.


Author(s):  
Жанна Владимировна Вечеркина ◽  
Наталья Александровна Шалимова ◽  
Наталия Владимировна Чиркова ◽  
Елена Анатольевна Андреева ◽  
Марина Николаевна Бобешко

Увеличивающаяся тенденция нуждаемости пациентов использования несъемных, съемных зубных протезов, ортодонтических и челюстно-лицевых апппаратов, наблюдающаяся в последние годы, сопряжена с многими проблемами. Использующиеся в практике ортопедической стоматологии зубные протезы и аппараты оказывают побочное действие на слизистую оболочку полости рта (механическое, химико-токсическое, сенсибилизирующее), что приводит к нарушению биоценоза, чрезмерному росту патогенных микроорганизмов и увеличению числа дрожжевых колоний. Установлено также, что при использовании адгезивных препаратов для улучшения фиксации съемных протезов статистически достоверно увеличивается число дрожжевых колоний при количественном посеве по сравнению с контрольной группой пациентов. Еще более сложная ситуация наблюдается у пациентов, уже имеющих хроническое нарушение соотношения нормальной и условно - патогенной микробиоты и нуждающихся в протезировании съемными пластиночными протезами. Эти пациенты, как правило, имеют неудовлетворительную гигиену полости рта и тяжелую сопутствующую патологию, чаще всего сахарный диабет или дисбактериоз ЖКТ. В настоящее время получены убедительные данные о важной роли иммунных нарушений в патогенезе развития кандидоинфекции и антифунгальной резистентности. В частности, дисфункции клеточного звена иммунной системы, что способствует хроническому, рецидивирующему и зачастую торпидному к терапии течению заболевания. Это, в свою очередь, требует выполнения научных исследований в направлении коррекции состояния микрофлоры ротовой полости пользующихся ортопедическими конструкциями. Данная обзорная работа посвящена актуальным на сегодняшний день вопросам применения пре-, пробиотических культур и синбиотиков в комплексном лечении больных с дисбиотическим изменением полости рта, пользующихся ортодонтическими конструкциями, лечебно-диагностическими аппаратами. Целью работы стал всесторонний анализ проблемы целесообразности и эффективности использования вышеназванных средств с точки зрения этиопатогенеза дисбиоза в полости рта The increasing trend of patients ' need to use non-removable, removable dentures, orthodontic and maxillofacial devices, observed in recent years, is associated with many problems. Dental prostheses and devices used in the practice of orthopedic dentistry have a side effect on the oral mucosa (mechanical, chemical-toxic, sensitizing), which leads to a violation of the biocenosis, excessive growth of pathogenic microorganisms and an increase in the number of yeast colonies. It was also found that the use of adhesive preparations to improve the fixation of removable prostheses statistically significantly increases the number of yeast colonies during quantitative seeding compared to the control group of patients. An even more difficult situation is observed in patients who already have a chronic violation of the relationship between normal and opportunistic microbiota and need prosthetics with removable plate prostheses. These patients usually have poor oral hygiene and severe comorbidities, most often diabetes mellitus or gastrointestinal dysbiosis. Currently, there is strong evidence of the important role of immune disorders in the pathogenesis of Candida infection and antifungal resistance. In particular, dysfunction of the cellular link of the immune system, which contributes to the chronic, recurrent and often torpid to therapy course of the disease. This, in turn, requires scientific research in the direction of correlation of the state of the oral microflora using orthopedic structures. This review is devoted to current issues of the use of pre - probiotic cultures and synbiotics in the complex treatment of patients with dysbiotic changes in the oral cavity, using orthodontic structures, medical and diagnostic devices. The aim of the work was a comprehensive analysis of the problem of expediency and effectiveness of the use of the above-mentioned means, from the point of view of the eiopathogenesis of dysbiosis in the oral cavity


2021 ◽  
Vol 15 (5) ◽  
pp. 39-43
Author(s):  
M. S. Yesayan ◽  
E. I. Selifanova ◽  
E. G. Margaryan ◽  
T. V. Beketova

Systemic sclerosis (SSc) can lead to pathological changes in the maxillofacial region, contributing to the violation of the microbiocenosis of the oral cavity with a predominance of pathogenic microflora.Objective: to study the composition of the oral microflora in patients with SSc. Patients and methods. The composition of the oral microflora was studied in 50 patients with SSc. The control group consisted of 50 subjects without rheumatic diseases. To assess the intensity of dental caries and the level of oral hygiene we used dental indices: the index of caries intensity (Decayed, Missing, and Filled Teeth (DMFT) and the hygienic index (OHI-S).Results and discussion. Microbiological examination in patients with SSc revealed pathogenic Staphylococcus aureus and Candida albicans > 10-6 CFU in equal percentage of cases (18.9%), which was significantly more frequent than in the control group (p=0.049). In the oral cavity in SSc, there were no representatives of normal microflora (lactobacilli). In patients with SSc, the DMFT index was 17.8±7.1 on average, and OHI-S – 2.3±0.7, which corresponds to a very high level of caries intensity and low indicators of oral hygiene, respectively. When analyzing the microflora of the oral cavity in 90% of cases, a dysbiotic shift of the 3rd degree was stated.Conclusion. It can hypothesized that the qualitative and quantitative composition of the microflora of the oral cavity affects the development and severity of inflammatory and destructive pathology of the periodontal and oral mucosa. It is necessary to develop and implement an adapted personal hygiene regimen, including cleansing of the tongue and administration of local probiotics, which, as part of complex therapy, can improve the results of SSc treatment.


2018 ◽  
Vol 8 (12) ◽  
pp. 90 ◽  
Author(s):  
Cristina Silva Sousa ◽  
Vanessa de Brito Poveda ◽  
Ruth Natalia Teresa Turrini

Objective: This study compared the effectiveness of an informational booklet on postoperative self-care, knowledge, anxiety and symptoms related to orthognathic surgery.Methods: This study is a randomized, single-blind, controlled clinical trial. This study was carried out from August 2013 to August 2015 and included 40 participants (20/group). The intervention group had routine postoperative surgeon guidance, and received a self-care booklet. Booklet information was explained by a nurse. The control group received only routine surgeon guidance. Knowledge and anxiety were compared pre- and postoperatively. Postoperative outcomes included self-care (oral hygiene, mobility and sensitivity, hydration of the lips, edema, and sleeping and breathing conditions), nutrition (eating ability and change in bodyweight), and pain (treatment required and a visual analog scale). Data were analyzed by general linear mixed models and mixed-effects models.Results: Knowledge was higher in intervention group compared to control group (p < .001). Anxiety assessment displayed no difference between groups. The intervention group had more halitosis (p = .003) and greater bodyweight loss (p = .002).Conclusions: The booklet increased knowledge of postoperative self-care, but did not lower anxiety. Halitosis and weight loss were higher in the intervention group, however, most outcomes were similar between groups.


2018 ◽  
Vol 34 (1-2) ◽  
pp. 26-37
Author(s):  
Rudi Ismail ◽  
Achiriul Bakri ◽  
Mohammad Nazir ◽  
Ryanto Haridawati

To evaluate the effectiveness of the standard practice of antibiotic prescribing in diarrheal diseases (DD) at Palembang General Hospital, we performed this single blind clinical trial. Subjects were children with DD, without E. histolytica.. or G. Iamblia in their stool, 6 to 59 months of age, seen at the OPD from May 20, 1991 until March 31, 1992. Antibiotic treatment (AT) was given to the treated group (n=289), and was withheld from the control group (n=298). The effectiveness of antibiotic treatment was measured by rate of reconsultation, need for subsequent AT, duration of diarrhea, vomiting, and fever as measured by home visitors. The treated group has a significantly shorter duration of diarrhea and a significant difference in the need for additional AT. Subjects whose diarrhea persist more than 7 days were significant statistically only in bloody diarrhea and in subjects whose fecal leukocytes were more than 9 per high power field. Profuse diarrhea and mother's anxiety were the main reasons for further consultation, which were strikingly greater in control than in treated group. Mothers seeked reconsultation 12.5 times more often for bloody diarrhea and 19.5 times for mucoid diarrhea plus fever. This study reconfirmed that AT in DD shortens the duration of diarrhea, diminishes the rate of reconsultation, and need for subsequent antibiotics in bloody and mucoid diarrhea.


2019 ◽  
Vol 36 (2) ◽  
pp. 135-142 ◽  
Author(s):  
Kyung-Hyo Do ◽  
Ho-Eun Park ◽  
Mi-Sun Kang ◽  
Jong-Tae Kim ◽  
Ji-Eun Yeu ◽  
...  

Weissella (W.) cibaria strain Chonnam Medical University (CMU) has shown oral colonizing ability and inhibitory effects on the formation of volatile sulfur compounds (VSCs) in vitro studies. The present study was conducted to analyze the effects of the W. cibaria CMU on canine oral health. Halitosis, calculus, plaque, gingivitis, and intraoral microbiota were assessed in 3 groups: control (maltodextrin), W. cibaria CMU low concentration (CMU-L, 2 × 107 colony forming unit [CFU]), and high-concentration (CMU-H, 2 × 109 CFU). Halitosis was analyzed using both organoleptic evaluation and measurement of VSCs. Intraoral microbiota were analyzed by real-time polymerase chain reaction. From week 4, the total VSC level in the CMU-H group (4.0 ± 1.30 ng/10 mL) was significantly lower than in the control group (6.3 ± 2.28 ng/10 mL). Significant reduction in methyl mercaptan in the CMU-treated groups was also observed. In addition, the plaque index in the CMU-treated groups was significantly decreased. The CMU-treated groups showed significant decreases in Fusobacterium nucleatum, Porphyromonas gingivalis, Prevotella intermedia, and Tannerella forsythia and demonstrated the colonizing ability of W. cibaria CMU in the oral cavity. We demonstrated that W. cibaria CMU suppresses halitosis, colonizes the oral cavity, and inhibits the proliferation of malodor-causing oral bacteria in beagles. According to these results, we expect that W. cibaria CMU could be a new oral hygiene solution by reducing VSC production and inhibiting the growth of oral harmful bacteria in companion animals.


2021 ◽  
Vol 2021 ◽  
pp. 1-7
Author(s):  
Fataneh Ghorbanyjavadpour ◽  
Vahid Rakhshan

Purpose. The literature regarding the treatment of posterior crossbites using a transpalatal arch (TPA) is scarce. Moreover, there is only one clinical study on the correction of unilateral crossbites using torque activation. This is an important clinical issue; therefore, this study was conducted to show the effects of an active Goshgarian TPA in correcting nonfunctional single-tooth unilateral crossbite. Methods. The present single-blind, randomized clinical trial examined 60 observations on 30 individuals with nonfunctional single-tooth unilateral crossbites in the first permanent molar area. Patients were randomly divided into two groups of “symmetric expansion” [control] and “expansion + torque activation” using Goshgarian TPAs [experimental]. The palatal arch was expanded at a rate of 2 mm/month, for 2–8 months. The average treatment durations were 157.9 and 117.1 days, respectively, for the control and experimental groups. Dentoskeletal alterations were assessed on dental records, posteroanterior frontal cephalographs, and occlusal radiographs taken before and after treatment. Changes induced by treatments in each group and differences between changes in both groups were analyzed statistically (α = 0.05). Results. The treatment duration was significantly shorter in the experimental group ( P < 0.05 ). The extent of dental displacement on the crossbite side was significant no matter what treatment was applied ( P < 0.001 ); no between-group difference was detected ( P > 0.05 ). Both treatments tilted the teeth in crossbite ( P < 0.001 ) without any between-group difference ( P > 0.05 ). The noncrossbite molar was displaced in the control group, whereas this did not occur in the experimental group (between-group P < 0.001 ). Conclusions. The Goshgarian TPA can be used with torque activation in order to deliver a more effective and faster correction of nonfunctional single-tooth unilateral crossbites with more favorable clinical results.


2016 ◽  
Vol 9 (4) ◽  
pp. 257
Author(s):  
Mahin Najafian ◽  
Ahmad Ahmadzadeh ◽  
Mojgan Barati ◽  
Mahin Bahadori ◽  
Zeinab Shajirat

<p>To the best of our knowledge, there is no prospective trial study assessing the management of β-thalassemia by blood transfusion in pregnancy. The aim of this study was to investigate the effect of blood transfusion on maternal and neonatal outcomes in pregnant patients with beta thalassemia minor and intermedia. We did this randomized, single –blind, controlled clinical trial on 36 pregnant women with β thalassemia minor and intermedia at two tertiary hospitals of Imam Khomeini and Shafa in Ahvaz, Iran from January 2016 to July 2016. Patients were randomly assigned to receive either packed cell or not (control group) during pregnancy (ratio 1:1). The main outcomes of interest transfusion times, abortion, preterm delivery, type of delivery (cesarean or vaginal), stillbirth, birth weight, low birth weight, very low birth weight, small for gestational age, low Apgar score (≤7) at 1 and 5 minutes, oligohydramnios, fetal anomalies , and NICU admission. The median of blood transfusion in intervention group during pregnancy was two blood units. The cesarean section rate did not significantly differ between the intervention and control groups (72.2% and 44.4%, respectively, P=0.2). Two infants in intervention group experienced with LBW, one with SGA, one with low Apgar score, two with oligohydramnios, and one with NICU admission, while none in control group experienced these complications. The mean birth weight of infants in intervention group was significantly lower than control group (P value 0.002). The comparison between two groups showed that the adverse maternal and neonatal outcomes, especially low birth weight and preterm delivery were higher in intervention group.</p>


2018 ◽  
Vol 29 (6) ◽  
pp. 583-591 ◽  
Author(s):  
Vail Natale Júnior ◽  
Francisley Ávila Souza ◽  
Eduardo Vedovatto ◽  
Renato Sussumu Nishioka ◽  
Pier Paolo Poli ◽  
...  

Abstract The purpose of this study was to evaluate the preservation of alveolar dimensions in human fresh extraction sockets filled with a composite bovine bone graft by means of design of single-blind randomized clinical trial. Forty participants had monoradicular teeth extracted (one teeth in each participant), and after were randomly divided into 2 groups: individuals whose fresh sockets were filled with the composite heterologous bone graft (Biomaterial Group), or with blood clot (Control Group). After extraction, the fresh sockets were measured at their greatest mesiodistal (MD) and bucco-lingual/palatal (BL/P) distance. Primary closure of the soft tissue was performed with a fibro-mucosal plug. After 120 post-operative days, the re-entry procedure was performed and the largest MD and BL/P measurements were again obtained to calculate the remodeling of the alveolar bone measured in percentage. In the biomaterial group, a percentage reduction of 1.62% and 3.29% in the MD and BL/P dimensions was observed 120 days after the extractions, whereas a reduction of 4.97% and 7.18% in the MD and BL/P dimensions occurred in the control group. There was a statistically significant difference (p<0.05) between the two groups for the bucco-palatal and mesiodistal measurements in the maxilla. In view of the results obtained, it can be concluded that composite bovine bone graft limited but did not impede alveolar bone remodeling.


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