LcaR: a regulatory switch from Pseudomonas aeruginosa for bioengineering alkane degrading bacteria

2022 ◽  
Author(s):  
Erandika H. Hemamali ◽  
Laksiri P. Weerasinghe ◽  
Hideaki Tanaka ◽  
Genji Kurisu ◽  
Inoka C. Perera
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Degrading Bacteria

scholarly journals Enhanced Biodegradation of Phthalic Acid Esters’ Derivatives by Plasticizer-Degrading Bacteria (Burkholderia cepacia, Archaeoglobus fulgidus, Pseudomonas aeruginosa) Using a Correction 3D-QSAR Model

2020 ◽  
Vol 17 (15) ◽  
pp. 5299
Author(s):  
Haigang Zhang ◽  
Chengji Zhao ◽  
Hui Na
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Burkholderia Cepacia ◽  
Energy Barrier ◽  
Phthalic Acid ◽  
Similarity Index ◽  
3D Qsar ◽  
Qsar Model ◽  
Dynamics Simulation ◽  
Archaeoglobus Fulgidus ◽  
Degrading Bacteria

A phthalic acid ester’s (PAEs) comprehensive biodegradability three-dimensional structure-activity relationship (3D-QSAR) model was established, to design environmentally friendly PAE derivatives, which could be simultaneously degraded by plasticizer-degrading bacteria, such as Burkholderia cepacia, Archaeoglobus fulgidus, and Pseudomonas aeruginosa. Only three derivatives of diethyl phthalate (DEP (DEP-27, DEP-28 and DEP-29)) were suited for their functionality and environmental friendliness, which had an improved stability in the environment and improved the characteristics (bio-toxicity, bioaccumulation, persistence, and long-range migration) of the persistent organic pollutants (POPs). The simulation inference of the microbial degradation path before and after DEP modification and the calculation of the reaction energy barrier exhibited the energy barrier for degradation being reduced after DEP modification and was consistent with the increased ratio of comprehensive biodegradability. This confirmed the effectiveness of the comparative molecular similarity index analysis (CoMSIA) model of the PAE’s comprehensive biodegradability. In addition, a molecular dynamics simulation revealed that the binding of the DEP-29 derivative with the three plasticizer-degradation enzymes increased significantly. DEP-29 could be used as a methyl phthalate derivative that synergistically degrades with microplastics, providing directional selection and theoretical designing for plasticizer replacement.

scholarly journals Biodegradation Styrofoam by Soil Bacteria from Sarimukti Cipatat Bandung Final Disposal Site

2020 ◽  
Vol 13 (2) ◽  
pp. 218-227
Author(s):  
Tri Rahayu Hidayat ◽  
Ida Indrawati ◽  
Tati Herlina
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Functional Groups ◽  
Bacillus Amyloliquefaciens ◽  
Soil Bacteria ◽  
Physical Analysis ◽  
Bacillus Firmus ◽  
Degrading Bacteria ◽  
Scanning Electron

AbstrakStyrofoam digunakan sebagai kemasan makanan atau minuman, dibentuk oleh stiren dan benzena. Migrasi benzena dari bahan kemasan ke makanan dapat menyebabkan berbagai penyakit. Cara untuk mengurangi limbah styrofoam adalah dengan mencari bakteri yang dapat mendegradasi styrofoam secara alami. Sumber potensial untuk menemukan bakteri tersebut adalah di Tempat Pembuangan Akhir Sarimukti. Metode penelitian ini menggunakan metode eksplorasi yang dianalisis secara deskriptif kualitatif. Tahapan  penelitian terdiri atas uji biodegradasi dengan metode Winogradsky Column, perhitungan persentase penurunan berat kering Styrofoam, analisis fisik dengan Scanning Electron Microscope (SEM), dan analisis perubahan gugus fungsi dengan FT-IR.Hasil penelitian ini ditemukan 4 spesies bakteri pendegradasi polistiren yaitu Pseudomonas aeruginosa, Bacillus amyloliquefaciens, Bacillus cereus, dan Bacillus firmus. Persentase penurunan berat kering polistiren menunjukkan pada minggu kedelapan mencapai 18,23% dan analisis fisik dengan Scanning Electron Microscope (SEM) menunjukkan bahwa proses degradasi oleh bakteri tanah menghasilkan pembentukan pori-pori di permukaan Styrofoam. Analisis gugus fungsi menunjukkan bahwa gugus fungsi menjadi lebih sederhana setelah degradasi dengan munculnya gugus fungsi C-O pada bilangan gelombang 1.030,02 cm-1. Bakteri pendegradasi polistiren dari tempat pembuangan akhir Sarimukti ini dapat direkomendasikan sebagai metode yang ramah lingkungan untuk mengurangi limbah styrofoam.Abstract Styrofoam used as packaging food or drinks, that are formed on styrene and benzene. The migration of benzene from packaging materials to food can cause various diseases. The way to reduce styrofoam waste is to look for bacteria that can degrade styrofoam naturally. The most potent source of finding bacteria is in the Sarimukti Final Landfill. This research method uses exploratory methods that are analyzed descriptively qualitative. The stages of the study consisted of biodegradation testing using the Winogradsky method, calculation of the percentage of weight loss of Styrofoam, physical analysis with Scanning Electron Microscope (SEM), and analysis of changes in functional groups with FTIR. The results of this study found 4 species of polystyrene degrading bacteria is Pseudomonas aeruginosa, Bacillus amyloliquefaciens, Bacillus cereus and Bacillus firmus. The percentage reduction in dry weight of polystyrene showed in the eighth week which reached 18.23% and physical analysis with Scanning Electron Microscope (SEM) indicates that the process of degradation by soil bacteria showed the formation of pores on the surface. Functional group analysis shows that functional groups become simpler after the degradation with the appearance of C-O functional groups at wave number 1,030.02 cm-1. These polystyrene degrading bacteria from Sarimukti landfills can be recommended as an environmentally friendly method for reducing styrofoam waste.

scholarly journals Isolation and Screening of Water Microbes for Decolourisation of Textile Dye Waste

2016 ◽  
Vol 11 (1) ◽  
pp. 296-300
Author(s):  
J. K Singh ◽  
R Ranjan ◽  
Pranay Pankaj
Keyword(s):  
Waste Water ◽  
Pseudomonas Aeruginosa ◽  
Bacillus Subtilis ◽  
Azo Dyes ◽  
Bacterial Species ◽  
Textile Dye ◽  
Human Beings ◽  
Degrading Bacteria ◽  
Vis Spectroscopy ◽  
Pre Treatment

Azo dyes are widely used in textile industry. Unused dyes, consisting mainly non biodegradable released along with waste water streams without any proper pre-treatment which cause nuisance for environment and accumulate in flora as well as fauna. These also exhibit allergic, carcinogenic and mutagenic properties for human beings. Isolation and screening of azo dye degrading bacteria are economic in biodegradation and detoxification. In the present study, 200 waste water samples were collected from dye-contaminated sites of textile industries and bacterial species such as Bacillus subtilis, Pseudomonas aeruginosa and Psuedomonas putida were isolated and identified. Evaluation of decolorizing properties of these bacteriae were done by UV-Vis spectroscopy (Amax 596 nm) in different concentrations using different carbon sources such as Hans’s medium and GYP medium. Maximum decolourisation of 0.1% azo dyes were recorded to be 89.0%, 91% and 86% in Hans medium containing charcoal source by Bacillus subtilis, Pseudomonas aeruginosa and Psuedomonas putida respectively at 24 hrs. These bacterial isolates may be utilized in large scale for pre-treatment for ecological balance by avoiding water pollution.

scholarly journals Biodegradation α-endosulfan and α-cypermethrin by Acinetobacter schindleri B7 isolated from the microflora of grasshopper (Poecilimon tauricola)

2021 ◽  
Author(s):  
Ozlem Gur Ozdal ◽  
Omer Faruk ALGUR
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Bacillus Megaterium ◽  
Biological Treatment ◽  
Minimal Salt Medium ◽  
Salt Medium ◽  
Negative Bacterium ◽  
Pesticide Degradation ◽  
Gram Negative ◽  
Degrading Bacteria ◽  
Brevibacillus Parabrevis

Abstract Extensive applications of pesticides have led to the contamination of ecosystem. Therefore, the isolation of new pesticide degrading bacteria is important. For the biodegradation of α-endosulfan and α-cypermethrin, new bacteria isolates were isolated from grasshopper (Poecilimon tauricola). According to different tests, these isolated bacteria were identified as Pseudomonas aeruginosa B5, Acinetobacter johnsonii B6, Acinetobacter schindleri B7, Bacillus megaterium B9 and Brevibacillus parabrevis B12. The first two of these bacteria have been isolated as those that can use only α-endosulfan and the last two only use α-cypermethrin. Moreover, A. schindleri B7 was determined to be able to degrade both pesticides. When glucose was added to non-sulfur medium containing α-endosulfan (100 mg/L) and minimal salt medium containing α-cypermethrin (100 mg/L), both pesticide degradation and bacterial growth was increased. As a result, A. schindleri, a new gram negative bacterium, can inevitably be used in the biological treatment of environments exposed to pesticides.

Biodegradation of tobacco waste by composting: Genetic identification of nicotine-degrading bacteria and kinetic analysis of transformations in leachate

2012 ◽  
Vol 66 (12) ◽  
Author(s):  
Felicita Briški ◽  
Nina Kopčić ◽  
Ivana Ćosić ◽  
Dajana Kučić ◽  
Marija Vuković
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Liquid Waste ◽  
Genetic Identification ◽  
Airflow Rate ◽  
Degrading Bacterium ◽  
Half Saturation Constant ◽  
Degrading Bacteria ◽  
Bacterium Strain ◽  
Monod Kinetic ◽  
Tobacco Waste

AbstractThe tobacco industry produces large quantities of solid and liquid waste. This waste poses a significant environmental problem, as some major components are harmful and toxic. The aim of this work is to isolate and identify the nicotine-degrading microorganisms in the composting of tobacco waste. The bioremediation process for the detoxification of waste was carried out in a column reactor at an airflow-rate of 0.4 L min−1 kg−1. The concentrations of nicotine and number of CFU in the samples taken from reactor were monitored over nineteen days. After nineteen days, 89.8 % of nicotine conversion was obtained. A nicotine-degrading bacterium, strain FN, was isolated from the composting mass and identified as Pseudomonas aeruginosa on the basis of morphology, 16S rDNA sequence, and the phylogenetic characteristics. To confirm that the isolated Pseudomonas aeruginosa FN is the actual nicotine degrader, batch experiments were performed using tobacco leachate. It was confirmed that the strain FN possesses a considerable capacity to degrade nicotine with simultaneous COD removal. The Monod kinetic model for single substrate was applied to obtain the substrate degradation rate and half saturation constant.

Isolation and Characterization of a Styrene Trimer Degrading Microorganism

2014 ◽  
Vol 1073-1076 ◽  
pp. 666-671
Author(s):  
Guang Chun Li ◽  
Chun Xiang Piao ◽  
Katsuhiko Saido ◽  
Seon Yong Chung
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Liquid Phase ◽  
Carbon Source ◽  
Sole Carbon Source ◽  
Isolation And Characterization ◽  
Degrading Bacteria ◽  
Ochrobactrum Intermedium ◽  
Complex Strain ◽  
Degrading Microorganism

Biodegradation of the styrene trimer was investigated, and its degrading bacteria were screened and isolated. Complex bacteria ST (strain ST1 and ST2) was isolated from contaminated soil by polystyrene and named by strain ST1 and ST2. ST1 and ST2 were identified by 16S rDNA and classified byOchrobactrum intermediumsp. andPseudomonas aeruginosasp., respectively. Biodegradation experiments were performed in batch and styrene trimer was used as a sole carbon source. Isolated two bacteria were used as degrading microorganisms. Initial liquid phase concentration of the styrene trimer was 50 mg/L. 95% of the styrene trimer was degraded in 17 days by the complex strain ST. The concentration was analyzed by using GC. Metabolites of bacteria were analyzed and three kinds of products that were identified by GC/MS.

scholarly journals ISOLASI BAKTERI DARI TANAH TEMPAT PEMBANGAN AKHIR (TPA) AIR SEBAKUL SEBAGAI AGEN BIODEGRADASI LIMBAH PLASTIK POLYETHYLENE

Alotrop ◽  
2020 ◽  
Vol 4 (2) ◽  
pp. 98-106
Author(s):  
Desy Purnama Sari ◽  
Hermansyah Amir ◽  
Rina Elvia
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Bacterial Isolate ◽  
Morphological Characteristics ◽  
Bacterial Isolation ◽  
Gram Staining ◽  
Chemistry Learning ◽  
Degrading Bacteria ◽  
Identification Of Bacteria ◽  
Time Variations

This study aims to obtain the bacteria of Pseudomonas from the land of TPA Air Sebakul Bengkulu City as a plastic degrading agent and measure the ability of bacteria to degrade Low Density Polyethylene (LDPE) and Oxium plastic. The research was conducted from February to August 2019, at the Laboratory of Biology and Chemistry Learning, Faculty of Teacher Training and Education, University of Bengkulu. Air Sebakul landfill samples were taken at the coordinate point 3o49 '27.8 "S 102o20 '48.4" E. Isolation of plastic degrading bacteria using selective media King's B Agar added with 2% Polyethylen Glycol (PEG) to test the ability to develop bacterial isolates in plastic-based media. The steps of this research are bacterial isolation, bacterial purification and bacterial selection, macroscopic and microscopic identification of bacteria (Gram staining) and plastic biodegradation test with Mineral Salt Agar (MSM) media. Determination of the% weight loss of LDPE and oxium plastics in the biodegradation process was carried out for 30 days with time variations of 10, 20 and 30 days. The results of bacterial isolation based on morphological characteristics and gram staining test of P-1 bacterial isolate have similarities with Pseudomonas aeruginosa. So that the P-1 bacterial isolate is thought to be a Pseudomonas aeruginosa bacterium. The bidoegradation of LDPE and oxium plastics with isolates of P-1 bacteria for 10, 20 and 30 days respectively was able to degrade oxium plastics by 2.43, 5.17 and 9.86% while LDPE plastics by 1.13, 2 and 1 , 17%.

scholarly journals TREATMENT PETROLEUM OIL CONTAMINATED SOIL BY INOCULATION OF DIFFERENT BACTERIAL STRAINS

2019 ◽  
Vol 2 (1) ◽  
pp. 1
Author(s):  
Pankaj Kumar Jain
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Contaminated Soil ◽  
Bacterial Count ◽  
Soil Samples ◽  
Total Bacterial Count ◽  
Soil Reaction ◽  
Available Phosphorus ◽  
Bacterial Strains ◽  
Degrading Bacteria ◽  
Petroleum Oil

Petroleum oil contains a large number of poly cyclic hydrocarbons (PAH's) that are toxic to living beings. The complete degradation of petroleum oil required a population of microorganisms in the soil. In the present investigation petroleum oil contaminated soil samples were incubated with four bacterial strains (Mycobacterium sp., Pseudomonas aeruginosa, Alcaligenes faecalis and Enterobacter cloacae) to study the bioremediation efficacy. The soil samples were analyzed for soil reaction (pH), soil moisture content, soil organic carbon (SOC), available phosphorus (P), total petroleum hydrocarbon content (TPH), total bacterial count (TBC) and total petroleum degrading bacteria at the interval of 0 days (initial), 2 weeks, 4 weeks, 6 weeks and 8 weeks prior and after treatment by bacteria. Values obtained reveals that there was a clear modulating effect of bacteria on above determinations. Maximum decrements in TPH (86%), soil pH (18.2%) and SOC (40%) were recorded in Pseudomonas aeruginosa inoculated samples.

scholarly journals Characterizations of novel pesticide-degrading bacterial strains from industrial wastes found in the industrial cities of Pakistan and their biodegradation potential

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e12211
Author(s):  
Noreen Asim ◽  
Mahreen Hassan ◽  
Farheen Shafique ◽  
Maham Ali ◽  
Hina Nayab ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Enrichment Culture ◽  
Culture Technique ◽  
Industrial Wastes ◽  
Bacterial Strains ◽  
Isolation And Identification ◽  
16S Rrna Analysis ◽  
Degrading Bacteria ◽  
Considerable Degradation ◽  
Different Strains

Background Lack of infrastructure for disposal of effluents in industries leads to severe pollution of natural resources in developing countries. These pollutants accompanied by solid waste are equally hazardous to biological growth. Natural attenuation of these pollutants was evidenced that involved degradation by native microbial communities. The current study encompasses the isolation of pesticide-degrading bacteria from the vicinity of pesticide manufacturing industries. Methods The isolation and identification of biodegrading microbes was done. An enrichment culture technique was used to isolate the selected pesticide-degrading bacteria from industrial waste. Results Around 20 different strains were isolated, among which six isolates showed significant pesticide biodegrading activity. After 16S rRNA analysis, two isolated bacteria were identified as Acinetobacter baumannii (5B) and Acidothiobacillus ferroxidans, and the remaining four were identified as different strains of Pseudomonas aeruginosa (1A, 2B, 3C, 4D). Phylogenetic analysis confirmed their evolution from a common ancestor. All strains showed distinctive degradation ability up to 36 hours. The Pseudomonas aeruginosa strains 1A and 4D showed highest degradation percentage of about 80% for DDT, and P. aeruginosa strain 3C showed highest degradation percentage, i.e., 78% for aldrin whilst in the case of malathion, A. baumannii and A. ferroxidans have shown considerable degradation percentages of 53% and 54%, respectively. Overall, the degradation trend showed that all the selected strains can utilize the given pesticides as sole carbon energy sources even at a concentration of 50 mg/mL. Conclusion This study provided strong evidence for utilizing these strains to remove persistent residual pesticide; thus, it gives potential for soil treatment and restoration.

scholarly journals Lubricant Oil Bioremediation by Rhodococcus erythropolis Bacteria and Indigenous Bacteria Isolated from Water Contaminated with Lubricant Oil

2020 ◽  
Vol 7 (1) ◽  
pp. 62-74
Author(s):  
Frentina Murti Sujadi ◽  
Yahya Yahya ◽  
Andi Kurniawan ◽  
Abd. Aziz Amin
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Rhodococcus Erythropolis ◽  
Polluted Water ◽  
Oil Contamination ◽  
Indigenous Bacteria ◽  
Used Oil ◽  
Lubricant Oil ◽  
Degrading Bacteria ◽  
Oil Concentration

The bioremediation system can be improved by using specific bacterial potential as oil-degrading bacteria which microorganisms can use hydrocarbons as a carbon source for their metabolic processes. The aim of this study is to identify R. erythropolis on degradation oil contamination and to obtain indigenous bacteria as new agent bacteria on bioremediation of oil contamination. The polluted water samples from used oil were taken from PPN Prigi, Trenggalek, East Java, Indonesia. The parameters considered were the detection and characterization of indigenous bacteria that degraded used oil. The density of bacteria was analyzed in the interval time of days 0, 2, 4, 6 and 7 and TPH was analyzed at final incubation. The results of this study indicate that the effectiveness of reducing oil concentration was used in testing the potential of bacteria from the highest was Pseudomonas aeruginosa which as indigenous bacteria isolated from water contaminated sites with application cell rate 1×108 cells/ml. It reduced of oil concentration up to 53%, and 1×106 cells/ml reduced oil concentration up to 47%. While, R. erythropolis with application cell rate 1×108 cells/ml reduced 47%. This result was found that Pseudomonas aeruginosa was effectively removed of oil concentration.

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