The impact of molecular markers in common bean through a scientometric approach

The current research was intended to comprehend hypoglycemic and anti-lipidaemic exercises of hydrated common bean (phaseolus Vulgaris L.) seed extracts on streptozotocin-induced diabetic albino rats. At a set portion fluctuate of 100, 200,300 mg/kg body weight of common bean extracts was orally directed as one portion for every day to polygenic disorder rats for a measure of thirty days. The impact of P.vulgaris L. on hypoglycemic, glycosylated hemoprotein (HbA1c) and blood serum lipid profile (Total cholesterin), Triglyceride (TG), very low-density lipoprotein (VLDL), low-density lipoprotein (LDL), High-density lipoprotein (HDL)) in plasma were estimated in the regular and diabetic induced rat. The outcomes demonstrated that quick glucose,serum TC, TG, LDL, VLDL, levels were significantly (p<0.05) attenuate, while blood serum HDL, the level was extensively (p<0.05) upgraded inside the diabetic rats. The inconclusive amount of pace of 300 mg/kg is more reasonable than that of a hundred mg/kg. Our examination so shows that Phaseolus vulgaris L has a powerful adversary to diabetic and anti-lipidaemic impacts on streptozotocin-induced diabetic rats, and results were comparable to reference drug glibenclamide.

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Incidence of base rot and wilt, sanitary severity and influence about seed pathology by cultivars of common bean (Phaseolus vulgaris)

Arquivos do Instituto Biológico ◽

10.1590/1808-1657000112018 ◽

2019 ◽

Vol 86 ◽

Author(s):

Mateus Paulino Braga ◽

Luana Machado Rezende ◽

Lucinete Martins Barbosa Estrela ◽

Natanael Marcos Lemes ◽

Anderson Rodrigues Rietjens ◽

...

Keyword(s):

Phaseolus Vulgaris ◽

Common Bean ◽

Test Method ◽

The Other ◽

Other Hand ◽

Seed Pathology ◽

Random Samples ◽

Physiological Quality ◽

The Impact ◽

The City

ABSTRACT: The objective of this work was to conduct a temporal evaluation of incidence of rot base and sanitary severity, and to relate the impact on the seed pathology of common bean cultivars. In the 2015-2016 harvest, in the city of Ipameri, Goiás, ten cultivars of common bean were evaluated (BRS Estilo©, BRS Pérola©, IPR Tangará©, IPR Tuiuiú©, IPR Uirapuru©, IAC Milênio©, Imperador©, IAC F3 R2©, IAC OTG© and IPR Campos Gerais©) and distributed into five blocks, totaling 40 experimental units. The incidence of wilt and base rot and sanitary severity were analyzed by taking ten random samples per block at 21, 28, 56, 63 and 69 days after planting. At the end of 120 days, a total of 20 plants were harvested per cultivar, and 250 seeds were harvested for application of the Blotter Test method. From 21 to 69 days after planting, the cultivars BRS Pérola© and IPR Campos Gerais© deserve to be highlighted for presenting the lowest incidence of wilt in the reproductive and vegetative cycles. The cultivar BRS Pérola© showed the lowest incidence of wilt and sanitary severity. In the analysis of harvested seeds, the cultivar BRS Pérola© presented high physiological quality for all evaluated parameters. On the other hand, Cramberry (OTG)© showed low physiological potential in germination and vigor tests.

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Adverse biological effects of Milan urban PM looking for suitable molecular markers of exposure

Chemical Industry and Chemical Engineering Quarterly ◽

10.2298/ciceq120206114m ◽

2012 ◽

Vol 18 (4-2) ◽

pp. 635-641 ◽

Cited By ~ 5

Author(s):

Paride Mantecca ◽

Maurizio Gualtieri ◽

Eleonora Longhin ◽

Giuseppina Bestetti ◽

Paola Palestini ◽

...

Keyword(s):

Molecular Markers ◽

Chemical Composition ◽

Biological Effects ◽

Detailed Knowledge ◽

Biological Responses ◽

Physico Chemical ◽

Biological Hazard ◽

The Impact

The results presented summarise the ones obtained in the coordinated research project Tosca, which extensively analysed the impact of Milan urban PM on human health. The molecular markers of exposure and effects of seasonally and size-fractionated PMs (summer and winter PM10, PM2.5) were investigated in in vitro (human lung cell lines) and in vivo (mice) systems. The results obtained by the analyses of cytotoxic, pro-inflammatory and genotoxic parameters demonstrate that the biological responses are strongly dependent upon the PM samples seasonal and dimensional variability, that ultimately reflect their chemical composition and source. In fact summer PM10, enriched in crustal elements and endotoxins, was the most cytotoxic and pro-inflammatory fraction, while fine winter PMs induced genotoxic effects and xenobiotic metabolizing enzymes (like CYP1B1) production, likely as a consequence of the higher content in combustion derived particles reach in PAHs and heavy toxic metals. These outcomes outline the need of a detailed knowledge of the PMs physico-chemical composition on a local scale, coupled with the biological hazard directly associated to PM exposure. Apparently this is the only way allowing scientists and police-makers to establish the proper relationships between the respirable PM quantity/quality and the health outcomes described by clinicians and epidemiologists.

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A meta-analysis of prognostic roles of molecular markers in papillary thyroid carcinoma

Endocrine Connections ◽

10.1530/ec-17-0010 ◽

2017 ◽

Vol 6 (3) ◽

pp. R8-R17 ◽

Cited By ~ 35

Author(s):

Huy Gia Vuong ◽

Uyen N P Duong ◽

Ahmed M A Altibi ◽

Hanh T T Ngo ◽

Thong Quang Pham ◽

...

Keyword(s):

Molecular Markers ◽

Papillary Thyroid Carcinoma ◽

Thyroid Carcinoma ◽

Random Effect ◽

Random Effect Model ◽

Disease Free Survival ◽

Papillary Thyroid ◽

Promoter Mutation ◽

Meta Analyses ◽

The Impact

The prognostic role of molecular markers in papillary thyroid carcinoma (PTC) is a matter of ongoing debate. The aim of our study is to investigate the impact of RAS, BRAF, TERT promoter mutations and RET/PTC rearrangements on the prognosis of PTC patients. We performed a search in four electronic databases: PubMed, Scopus, Web of Science and Virtual Health Library (VHL). Data of hazard ratio (HR) and its 95% confidence interval (CI) for disease-specific survival (DSS) and disease-free survival (DFS) were directly obtained from original papers or indirectly estimated from Kaplan–Meier curve (KMC). Pooled HRs were calculated using random-effect model weighted by inverse variance method. Publication bias was assessed by using Egger’s regression test and visual inspection of funnel plots. From 2630 studies, we finally included 35 studies with 17,732 patients for meta-analyses. TERT promoter mutation was significantly associated with unfavorable DSS (HR = 7.64; 95% CI = 4.00–14.61) and DFS (HR = 2.98; 95% CI = 2.27–3.92). BRAF mutations significantly increased the risk for recurrence (HR = 1.63; 95% CI = 1.27–2.10) but not for cancer mortality (HR = 1.41; 95% CI = 0.90–2.23). In subgroup analyses, BRAF mutation only showed its prognostic value in short-/medium-term follow-up. Data regarding RAS mutations and RET/PTC fusions were insufficient for meta-analyses. TERT promoter mutation can be used as an independent and reliable marker for risk stratification and predicting patient’s outcomes. The use of BRAF mutation to assess patient prognosis should be carefully considered.

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How to Choose a Good Marker to Analyze the Olive Germplasm (Olea europaea L.) and Derived Products

Genes ◽

10.3390/genes12101474 ◽

2021 ◽

Vol 12 (10) ◽

pp. 1474

Author(s):

Sara Sion ◽

Michele Antonio Savoia ◽

Susanna Gadaleta ◽

Luciana Piarulli ◽

Isa Mascio ◽

...

Keyword(s):

Molecular Markers ◽

Olea Europaea ◽

Dna Analysis ◽

Olive Tree ◽

Olea Europaea L ◽

Varietal Identification ◽

Olive Cultivars ◽

History Of ◽

The Impact ◽

Olea Europaéa

The olive tree (Olea europaea L.) is one of the most cultivated crops in the Mediterranean basin. Its economic importance is mainly due to the intense production of table olives and oil. Cultivated varieties are characterized by high morphological and genetic variability and present a large number of synonyms and homonyms. This necessitates the introduction of a rapid and accurate system for varietal identification. In the past, the recognition of olive cultivars was based solely on analysis of the morphological traits, however, these are highly influenced by environmental conditions. Therefore, over the years, several methods based on DNA analysis were developed, allowing a more accurate and reliable varietal identification. This review aims to investigate the evolving history of olive tree characterization approaches, starting from the earlier morphological methods to the latest technologies based on molecular markers, focusing on the main applications of each approach. Furthermore, we discuss the impact of the advent of next generation sequencing and the recent sequencing of the olive genome on the strategies used for the development of new molecular markers.

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Impacts of Phosphorus as Soil Application on Growth, Yield and some Chemical Constitutes of Common Bean Plants Grown under Saline Soil Conditions

Sustainable Food Production ◽

10.18052/www.scipress.com/sfp.7.24 ◽

2020 ◽

Vol 7 ◽

pp. 24-36

Author(s):

Mohamed A. Seif El-Yazal ◽

Ahmed A. El-Shewy ◽

Kariman E.S. Abdelaal ◽

Mostafa M. Rady

Keyword(s):

Chlorophyll Fluorescence ◽

Common Bean ◽

Photosynthetic Pigments ◽

Seed Weight ◽

Growth Traits ◽

Saline Soil ◽

Growth Yield ◽

Soil Conditions ◽

Total Carotenoids ◽

The Impact

This study aimed to assess the impact of calcium superphosphate (P; 0, 100, and 200 kg per feddan) as soil amendments, in addition to the recommended P, on the growth traits, green and dry yields characteristics, leaf photosynthetic pigments, chlorophyll fluorescence, and leaf contents of nutrients of common bean (Phaseolus vulgaris L., cv. “Bronco”) plants grown under saline soil conditions. Two field trials were conducted at the Experimental Farm of Faculty of Agriculture, Fayoum University during the 2016 and 2017 summer seasons. The obtained results showed that, Na+ content was significantly declined, while the all other tested parameters such as growth characteristics (i.e., shoot length, number of leaves per plant, area of leaves per plant, and shoot fresh and dry weights), yield characteristics of green pods and dry seeds (i.e., average pod weight, number of pods per plant, pods weight per plant, dry seed weight per plant and 100-seed weight), leaf photosynthetic pigments (i.e., total chlorophylls, total carotenoids) contents and leaf chlorophyll fluorescence (i.e., Fv/Fm and PI), leaf contents of N, P, K+, and Ca2+, and the ratios of K+/Na+, Ca2+/Na+ and K++Ca2+/Na+ were significantly increased by the two tested P treatments compared to the controls (without more P than the recommended). The all tested treatments conferred, approximately, the same results. Therefore, results of this study recommend using P at 100 kg per feddan above the recommended dose to optimize the common bean performance in saline soils.

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REQUIREMENT OF SUPPLEMENTAL IRRIGATION FOR DRY SEASON COMMON BEAN IN GOIÁS

Irriga ◽

10.15809/irriga.2015v1n2p57 ◽

2015 ◽

Vol 1 (2) ◽

pp. 57-66 ◽

Cited By ~ 1

Author(s):

Alexandre Bryan Heinemann ◽

Luís Fernando Stone

Keyword(s):

Phaseolus Vulgaris ◽

Grain Yield ◽

Simulation Model ◽

Common Bean ◽

Water Deficit ◽

Dry Season ◽

Supplemental Irrigation ◽

Dry Bean ◽

Irrigation Depth ◽

The Impact

REQUIREMENT OF SUPPLEMENTAL IRRIGATION FOR DRY SEASON COMMON BEAN IN GOIÁS ALEXANDRE BRYAN HEINEMANN1 E LUÍS FERNANDO STONE2 1 Embrapa Arroz e Feijão, Santo Antônio de Goiás, GO, [email protected] Embrapa Arroz e Feijão, Santo Antônio de Goiás, GO, [email protected] 1 ABSTRACT Common bean crop is grown in Goiás state in three crop seasons, "wet”, “dry”, and autumn/winter. In "dry" crop season, common bean yield is lower and it can be attributed in large part to the water deficit during its cycle. The use of supplemental irrigation can be an alternative to overcome this stress and increase grain yield. The objective of this study was to quantify the impact of water stress in "dry" season common bean and the irrigation depth required to mitigate this stress, using the CSM-CROPGRO-Dry bean simulation model. The model was calibrated and evaluated against observed data set for Pérola cultivar. Grain yield for six sowing dates (10/1, 20/01, 30/1, 10/2, 20/2, and 28/2) and three soil classes (Oxisol, Ultisol, and Inceptisol) were simulated considering growth under rainfall and growth with supplemental irrigation, using daily climatic data of 26 weather stations in Goiás for the period of 33 years. The relative impact of water deficit, estimated by the grain yield gap in both cases, was 48%. Soil classes had low impact on average values of irrigation depth required as well as on irrigated grain yield. The irrigation depth required increased from 70 to 157 mm and the grain yield decreased from 3,813 to 3,510 kg ha-1 with the advance of sowing date. Keywords: Phaseolus vulgaris, water deficiency, simulation model. HEINEMANN, A.B.; STONE, L.F.REQUERIMENTO DE IRRIGAÇÃO SUPLEMENTAR PARA O FEIJOEIRO DA SAFRA DA SECA EM GOIÁS 2 RESUMO O feijoeiro é cultivado em Goiás em três safras, “águas”, “seca” e outono/inverno. Na safra da “seca”, com semeadura de 01/01 a 28/02, a produtividade do feijoeiro é menor e pode ser atribuída, em grande parte, a ocorrência de deficiência hídrica durante o seu ciclo. O uso de irrigação suplementar pode ser uma alternativa para superar esse estresse e incrementar a produtividade. O objetivo deste trabalho foi quantificar o impacto da deficiência hídrica no feijão da “seca” e a lâmina de irrigação requerida para mitigar essa deficiência, utilizando o modelo de simulação CSM-CROPGRO-Dry bean. O modelo foi calibrado e validado por meio da comparação de dados observados e simulados para a cultivar Pérola. Foram simuladas a produtividade para seis datas de semeadura (10/1, 20/01, 30/1, 10/2, 20/2 e 28/2) e três classes de solo (Latossolo, Cambissolo e Argissolo), considerando o crescimento sob precipitação pluvial ou com o uso da irrigação suplementar, utilizando dados climáticos diários de 26 estações meteorológicas de Goiás para o período de 33 anos. O impacto relativo da deficiência hídrica, estimado pela diferença de produtividade nas duas situações, foi de 48%. As classes de solo tiveram pouco impacto na lâmina media requerida como também na produtividade irrigada. A lâmina média de irrigação requerida aumenta com o avanço da data de semeadura, variando de 70 a 157 mm, e a produtividade decresce de 3.813 a 3.510 kg ha-1. Palavras-chave: Phaseolus vulgaris, deficiência hídrica, modelo de simulação.

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The Healthy and Diseased Equine Endometrium: A Review of Morphological Features and Molecular Analyses

Animals ◽

10.3390/ani10040625 ◽

2020 ◽

Vol 10 (4) ◽

pp. 625 ◽

Cited By ~ 2

Author(s):

Sandra Schöniger ◽

Heinz-Adolf Schoon

Keyword(s):

Molecular Markers ◽

Microscopic Examination ◽

Molecular Mechanisms ◽

Plasma Cells ◽

Endometrial Biopsy ◽

Diagnostic Biomarkers ◽

Molecular Analyses ◽

Horse Breeding ◽

Health And Disease ◽

The Impact

Mares are seasonally polyestric. The breeding season in spring and summer and the winter anestrus are flanked by transitional periods. Endometrial diseases are a frequent cause of subfertility and have an economic impact on the horse breeding industry. They include different forms of endometrosis, endometritis, glandular maldifferentiation, and angiosis. Except for suppurative endometritis, these are subclinical and can only be diagnosed by the microscopic examination of an endometrial biopsy. Endometrosis is characterized by periglandular fibrosis and nonsuppurative endometritis by stromal infiltration with lymphocytes and plasma cells. The pathogenesis of endometrosis and nonsuppurative endometritis is still undetermined. Some mares are predisposed to persistent endometritis; this has likely a multifactorial etiology. Glandular differentiation has to be interpreted under consideration of the season. The presence of endometrial diseases is associated with alterations in the expression of several intra- and extracellular molecular markers. Some of them may have potential to be used as diagnostic biomarkers for equine endometrial health and disease. The aim of this review is to provide an overview on pathomorphological findings of equine endometrial diseases, to outline data on analyses of cellular and molecular mechanisms, and to discuss the impact of these data on reproduction and treatment.

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Effect of process-induced common bean hardness on structural properties of in vivo generated boluses and consequences for in vitro starch digestion kinetics

British Journal Of Nutrition ◽

10.1017/s0007114519001624 ◽

2019 ◽

Vol 122 (04) ◽

pp. 388-399 ◽

Cited By ~ 6

Author(s):

Andrea Pallares Pallares ◽

Bram Loosveldt ◽

Solomon N. Karimi ◽

Marc Hendrickx ◽

Tara Grauwet

Keyword(s):

Common Bean ◽

Structural Properties ◽

Kinetic Modelling ◽

Mechanical Degradation ◽

Common Beans ◽

Starch Digestion ◽

Digestion Kinetics ◽

The Impact

AbstractIn the present study, we evaluated the effect of process-induced common bean hardness on structural properties ofin vivogenerated boluses and the consequences forin vitrostarch digestion. Initially, the impact of human mastication on the particle size distribution (PSD) of oral boluses from common beans with different process-induced hardness levels was investigated through a mastication study. Then the effect of structural properties of selected boluses onin vitrostarch digestion kinetics was assessed. For a particular process-induced hardness level, oral boluses had similar PSD despite differences in masticatory parameters between participants of the mastication study. At different hardness levels, a clear effect of processing (P<0·0001) was observed. However, the effect of mastication behaviour (P=0·1141) was not significant. Two distinctive fractions were present in all boluses. The first one was a cotyledon-rich fraction consisting of majorly small particles (40–125 µm), which could be described as individual cells based on microscopic observations. This fraction increased with a decrease in process-induced hardness. The second fraction (>2000 µm) mostly contained seed coat material and did not change based on hardness levels. Thein vitrostarch digestion kinetics of common bean boluses was only affected by process-induced hardness. After kinetic modelling, significant differences were observed between the reaction rate constant of boluses generated from the hardest beans and those obtained from softer ones. Overall this work demonstrated that thein vitronutritional functionality of common beans is affected to a greater extent by structural properties induced by processing than by mechanical degradation in the mouth.

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