scholarly journals Evaluation of two wild castor (Ricinus communis L.) accessions for cadmium tolerance in relation to antioxidant systems and lipid peroxidation

2021 ◽  
Author(s):  
Akwasi Yeboah ◽  
Jiannong Lu ◽  
Shuailei Gu ◽  
Haiyan Liu ◽  
Yuzhen Shi ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activities ◽  
Growth Parameters ◽  
Ricinus Communis ◽  
Dry Weight ◽  
Antioxidant Systems ◽  
Control Group ◽  
Tolerance Index ◽  
Cd Stress ◽  
Mda Content

AbstractThe present study was conducted to assess the effect of toxicity of cadmium (Cd) on growth, tolerance index (TI), antioxidant activities, and malondialdehyde (MDA) content in two contrasting wild castor accessions (16-024 and S2-4) via hydroponic experiment (0 and 100 mg/L Cd). The results showed that Cd significantly reduced the growth rate, seedling height, root length, and shoot length of the castor accessions compared to the control, with the Cd effect being more severe in S2-4 than in 16-024. In addition, biomass response including the root and shoot fresh weight and root dry weight decreased in both accessions compared to the control. Compared to the control group, the shoot dry weight of accession S2-4 declined by 21.7%, whereas there was no change in 16-024, suggesting a level of tolerance in 16-024. Analysis of TI on all the growth parameters and biomass content revealed that accession 16-024 was highly tolerant to Cd stress than S2-4. The results further revealed that the expression of the antioxidant enzymes, viz., superoxide dismutase (SOD), catalase (CAT), non-enzymatic antioxidant, glutathione, and MDA content, was influenced by genotype. S2-4 exhibited a higher antioxidant activity (SOD, CAT) and lipid peroxidation activity than 16-024, indicative of oxidative damage from Cd stress.

scholarly journals Evaluation of Two Wild Castor (Ricinus Communis L.) Accessions for Cadmium Tolerance in Relation to Antioxidant Systems and Lipid Peroxidation

2021 ◽  
Author(s):  
Akwasi Yeboah ◽  
Jiannong Lu ◽  
Shuailei Gu ◽  
Liu Haiyan ◽  
Yuzhen Shi ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activities ◽  
Growth Parameters ◽  
Ricinus Communis ◽  
Dry Weight ◽  
Antioxidant Systems ◽  
Tolerance Index ◽  
Cd Stress ◽  
Shoot Dry Weight ◽  
Mda Content

Abstract The present study was conducted to assess the effect of toxicity of cadmium (Cd) on growth, tolerance index (TI), antioxidant activities, and malondialdehyde (MDA) content in two contrasting wild castor accessions (16–024 and S2-4) via hydroponic experiment (0 and 100 mg/L Cd). The results showed that Cd significantly reduced the growth rate, seedling height, root length, and shoot length of the castor accessions compared to control, with the Cd effect being more severe in S2-4 than in 16–024. In addition, biomass response including the root and shoot fresh weight and root dry weight decreased in both accessions compared to control. Shoot dry weight of 16–024 increased (p > 0.05) by 2.04 % compared to S2-4 which declined by 21.07 %, suggesting a minimal effect of Cd and a level of tolerance in 16–024. Analysis of TI on all the growth parameters and biomass content revealed that accession 16–024 was highly tolerant to Cd stress than S2-4. The results further revealed that the expression of the antioxidant enzymes viz., superoxide dismutase (SOD), catalase (CAT), non-enzymatic antioxidant; glutathione, and MDA content, were influenced by genotype. S2-4 exhibited a higher antioxidant activity (SOD, CAT) and lipid peroxidation activity than 16–024, indicative of oxidative damage from Cd stress.

scholarly journals Growth and Antioxidant Responses in Iron-Biofortified Lentil under Cadmium Stress

Toxics ◽  
2021 ◽  
Vol 9 (8) ◽  
pp. 182
Author(s):  
Ruchi Bansal ◽  
Swati Priya ◽  
Harsh Kumar Dikshit ◽  
Sherry Rachel Jacob ◽  
Mahesh Rao ◽  
...  
Keyword(s):  
Growth Parameters ◽  
Dry Weight ◽  
Cadmium Stress ◽  
Antioxidant Enzyme Activities ◽  
Limited Information ◽  
Cd Stress ◽  
Fresh Weight ◽  
Cd Uptake ◽  
Cd Tolerance ◽  
Controlled Conditions

Cadmium (Cd) is a hazardous heavy metal, toxic to our ecosystem even at low concentrations. Cd stress negatively affects plant growth and development by triggering oxidative stress. Limited information is available on the role of iron (Fe) in ameliorating Cd stress tolerance in legumes. This study assessed the effect of Cd stress in two lentil (Lens culinaris Medik.) varieties differing in seed Fe concentration (L4717 (Fe-biofortified) and JL3) under controlled conditions. Six biochemical traits, five growth parameters, and Cd uptake were recorded at the seedling stage (21 days after sowing) in the studied genotypes grown under controlled conditions at two levels (100 μM and 200 μM) of cadmium chloride (CdCl2). The studied traits revealed significant genotype, treatment, and genotype × treatment interactions. Cd-induced oxidative damage led to the accumulation of hydrogen peroxide (H2O2) and malondialdehyde in both genotypes. JL3 accumulated 77.1% more H2O2 and 75% more lipid peroxidation products than L4717 at the high Cd level. Antioxidant enzyme activities increased in response to Cd stress, with significant genotype, treatment, and genotype × treatment interactions (p < 0.01). L4717 had remarkably higher catalase (40.5%), peroxidase (43.9%), superoxide dismutase (31.7%), and glutathione reductase (47.3%) activities than JL3 under high Cd conditions. In addition, L4717 sustained better growth in terms of fresh weight and dry weight than JL3 under stress. JL3 exhibited high Cd uptake (14.87 mg g−1 fresh weight) compared to L4717 (7.32 mg g−1 fresh weight). The study concluded that the Fe-biofortified lentil genotype L4717 exhibited Cd tolerance by inciting an efficient antioxidative response to Cd toxicity. Further studies are required to elucidate the possibility of seed Fe content as a surrogacy trait for Cd tolerance.

Expression Analysis of 4-Hydroxynonenal Modified Proteins in Schizophrenia Brain; Relevance to Involvement in Redox Dysregulation

2021 ◽  
Vol 18 ◽  
Author(s):  
Sobia Manzoor ◽  
Ayesha Khan ◽  
Beena Hasan ◽  
Shamim Mushtaq ◽  
Nikhat Ahmed
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Thiobarbituric Acid ◽  
Brain Regions ◽  
Protein Adducts ◽  
Antioxidant Systems ◽  
Control Group ◽  
Tbars Level ◽  
Elevated Expression ◽  
Modified Proteins

Background: Oxidative damage contributes to the pathophysiology of schizophrenia (SZ). Redox imbalance may lead to increased lipid peroxidation, which produces toxic aldehydes like 4-hydroxynonenal (4-HNE) ultimately leading to oxidative stress. Conversely, implications of oxidative stress points towards an alteration in HNE-protein adducts and activities of enzymatic and antioxidant systems in schizophrenia. Objectives: Present study focuses on identification of HNE-protein adducts and its related molecular consequences in schizophrenia pathology due to oxidative stress, particularly lipid peroxidation. Material and Methods: Oxyblotting was performed on seven autopsied brain samples each from cortex and hippocampus region of schizophrenia patients and their respective normal healthy controls. Additionally, thiobarbituric acid substances (TBARS), reduced glutathione (GSH) levels and catalase (CAT) activities associated with oxidative stress, were also estimated. Results: Obtained results indicates substantially higher levels of oxidative stress in schizophrenia patients than healthy control group represented by elevated expression of HNE-protein adducts. Interestingly, hippocampus region of schizophrenia brain shows increased HNE protein adducts compared to cortex. An increase in catalase activity (4.8876 ± 1.7123) whereas decrease in antioxidant GSH levels (0.213 ± 0.015µmol/ml) have been observed in SZ brain. Elevated TBARS level (0.3801 ± 0.0532ug/ml) were obtained in brain regions SZ patients compared with their controls that reflects an increased lipid peroxidation (LPO). Conclusion: Conclusion: We propose the role of HNE modified proteins possibly associated with the pathology of schizophrenia. Our data revealed increase lipid peroxidation as a consequence of increased TBARS production. Furthermore, altered cellular antioxidants pathways related to GSH and CAT also highlight the involvement of oxidative stress in schizophrenia pathology.

scholarly journals Effects of cadmium-induced oxidative stress on growth and nitrogen assimilation in blackgram [Vigna mungo (L.) Hepper]

2013 ◽  
Vol 58 (1) ◽  
pp. 31-39
Author(s):  
Mohammad Mobin
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Nitrogen Assimilation ◽  
Dry Weight ◽  
Vigna Mungo ◽  
Cd Stress ◽  
Cd Accumulation ◽  
Shoot Dry Weight ◽  
Activity Of Enzymes ◽  
Consequent Increase

Cadmium (Cd) accumulation, oxidative damage, and nitrogen metabolism were studied in roots and leaves of 30-d-old blackgram plants [Vigna mungo (L.) Hepper], grown in a mixture of soil and compost (3:1) with different Cd concentrations. Significant reductions in both root and shoot dry weight were noted. The concentration of Cd in roots and leaves increased with increasing Cd levels. The level of lipid peroxidation elevated with a consequent increase in H2O2 content under Cd stress in both plant organs. The activity of enzymes mediating the nitrogen assimilation in roots and leaves was greatly reduced in the presence of Cd, except glutamate dehydrogenase (GDH) which showed a significant increase.

A Study on the Antioxidant Activity and Tissues Selective Inhibition of Lipid Peroxidation by Saponins from the Roots of Platycodon grandiflorum

2009 ◽  
Vol 37 (05) ◽  
pp. 967-975 ◽  
Author(s):  
Xian-Jun Fu ◽  
Hong-Bing Liu ◽  
Peng Wang ◽  
Hua-Shi Guan
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Selective Inhibition ◽  
Antioxidant Systems ◽  
Platycodon Grandiflorum ◽  
Platycodi Radix ◽  
Antioxidant Effects ◽  
Different Tissues ◽  
Selective Effects

Platycodi Radix is the root of Platycodon grandiflorum (Jacq.) A. DC and has been used as a traditional medicine in China. According to the theory of traditional Chinese medicine, Platycodi Radix (PR) possesses the character of lung meridian tropism (Guijing) and has selective effects on the lung and respiratory system. The aim of this study was to confirm the antioxidant effects of saponins from Platycodi Radix (PRS), with emphasis on its selective inhibition of lipid peroxidation in different tissues. The hydroxyl radical scavenging activity was determined by a H2O2/Fe2+ system; a modified thiobarbituric acid reactive species assay was used to measure the lipid peroxide in rats' tissues. These antioxidant activities were compared to ascorbic acid (Vc). The results showed that PRS had antioxidant activities in various antioxidant systems. And the inhibition capability of lipid peroxidation of PRS and Vc were excellent, but differed greatly in different tissues. These results suggested that PRS had antioxidant effects and selective inhibition of lipid peroxidation. It indicated that the mechanism of Platycodi Radix to treat some diseases might be related to its antioxidant activity, especially its tissue selective effects. However, further study is needed.

scholarly journals Effect of electric field on seed germination and growth parameters of chickpea Cicer arietinum L.

2020 ◽  
Vol 10 (4) ◽  
pp. 12-16
Author(s):  
A. Afrasiyab ◽  
J. Zafar ◽  
H. Muhmmad
Keyword(s):  
Seed Germination ◽  
Electric Field ◽  
Cicer Arietinum ◽  
Root Length ◽  
Growth Parameters ◽  
Dry Weight ◽  
Control Group ◽  
Cicer Arietinum L ◽  
Number Of Leaves ◽  
Germination And Growth

Cicer arietinum (L.) seed were exposed to electric field in the soil via electrodes. Five different EF were (3V,6V, 9V, 12V, and 0V) were induced 10 min after 24hours for 100 days, each treatment was consisting of an isolated gathering of plants. The rate of seed germination was enhanced to the control group. Plant height, root length, number of leaves, number of flowers, dry weight of the plant, and seed weight were measured at harvesting. Plants height by the influence of 3V, 6V, 9V and 12V were found to be significant in all treated groups than the control group, having an increase of 25.5%, 30.5%, 11.8%, and 17.1%. Similarly, root length was significantly increased than the control group, being increased in 3V, 6V and 12V by 28.6%, 24.0%, 3.0% and retarded in 9V by -3.0%. Leaves numbers were significantly higher than the control group, being increased by 25.3%, 25.2%, 15%, and 19.3% respectively. There was no centrality contrast found indifference in, number of flowers, the dried weight of plant and weight of seeds respectively.

scholarly journals Effects of Soybean Isoflavone and Astragalus Polysaccharide Mixture on Colostrum Components, Serum Antioxidant, Immune and Hormone Levels of Lactating Sows

Animals ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 132
Author(s):  
Hongzhi Wu ◽  
Ji Yang ◽  
Sibo Wang ◽  
Xin Zhang ◽  
Jinwang Hou ◽  
...  
Keyword(s):  
Antioxidant Activities ◽  
Basal Diet ◽  
Control Group ◽  
Hormone Levels ◽  
Soybean Isoflavone ◽  
Astragalus Polysaccharide ◽  
Significant Difference ◽  
Lactating Sows ◽  
Group A ◽  
Mda Content

The objectives of this study were to investigate the effects of soybean isoflavone (SI) and astragalus polysaccharide (APS) mixture on the colostrum components, serum antioxidant, immune and hormone levels of lactating sows. A total of 72 healthy Yorkshire × Landrace lactating sows, were randomly divided into four treatments with six replicates and three lactating sows for each replicate. The control group was fed the basal diet, while the experimental groups were fed the basal diet with 100, 200 and 300 mg/kg SI and APS mixture in the form of powder, respectively. Compared with the control group, (a) the total lactation yield of the 200 mg/kg group was significantly higher (p < 0.05) at 21 days, (b) there was no significant difference in colostrum composition, (c) TG, CHO and MDA content in each treatment group were significantly decreased (p < 0.05), (d) IgA, GH, IGF-1, TNF-α and SOD contents in the 200 mg/kg group were significantly increased (p < 0.05). The SI and APS mixture could improve the average daily feed intake, lactation yield, serum antioxidant activities, immune function, and hormone levels of lactating sows, and the optimum dosage in this study was 200 mg/kg.

scholarly journals Research of indicators of pro- and antioxidant system in spermoplasm in violation of fertility in men

Urology ◽  
2021 ◽  
Vol 25 (2) ◽  
Author(s):  
O.D. Melenevsky ◽  
O.M. Chaika ◽  
O.V. Tretyakova
Keyword(s):  
Oxidative Stress ◽  
Male Infertility ◽  
Protection System ◽  
Reproductive Age ◽  
Antioxidant Systems ◽  
Control Group ◽  
Antioxidant Protection ◽  
Study Results ◽  
Mda Content ◽  
Antioxidant Protection System

The article presents the study results of marker indicators of pro- and antioxidant spermoplasm systems among men of reproductive age with various types of infertility. It is shown that patients who were diagnosed with “secretory male infertility”, had the level of MDA content that was exceeding control indicators in 1.2 times (aЈ0,05) with simultaneous activation of SOD (in 132.2%, aЈ0,05) against the background of slight CAT activity decreasing. The SOD/CAT ratio increased in 1.44 times compared to the control, and the activity of GAOS enzymes was not significantly different from the control group. The condition of  pro- and antioxidant protection system can be classified as “activated” due to “SOD + CAT” link with imbalance in the system, which can lead to progression of cytotoxic effects. The patients’ segmentation who were diagnosed with “excretory-toxic male infertility” into three subgroups by MDA content in spermoplasm made it possible to establish that the first subgroup was characterized by “compensatory activation” mainly of GAOS against the background of decreasing MDA content, in the second subgroup - indicators of the pro- and anti-radical protection system did not have reliable differences comparing with control results. The third subgroup showed increase of MDA content (66.1%, aЈ0,01) and activity decrease of all anti-radical protection systems. The state of pro- and antioxidant protection system in this subgroup can be classified as “oxidative stress.” The indicators study of pro-antioxidant systems in spermoplasm among patients with various types of male infertility will allow to carry out pathogenetically justified prevention and correction of conditions, which are accompanied by development of oxidative stress.

scholarly journals Growth of Falcataria moluccana and Albizia chinensis seedling under aluminum exposure

2021 ◽  
Vol 22 (9) ◽  
Author(s):  
Mohammad Agus Salim ◽  
LULUK SETYANINGSIH ◽  
IMAM WAHYUDI ◽  
SRI WILARSO BUDI
Keyword(s):  
Root Length ◽  
Acid Soils ◽  
Dry Weight ◽  
Limiting Factors ◽  
Tolerance Index ◽  
Exposure Treatment ◽  
Aluminum Exposure ◽  
Al Content ◽  
Randomized Design ◽  
Mda Content

Abstract. Salim MA, Setyaningsih L, Wahyudi I, Budi SW. 2021. Growth of Falcataria moluccana and Albizia chinensis seedling under aluminum exposure. Biodiversitas 22: 3693-3701. Aluminum (Al) is an element found in acid soils and is one of the limiting factors for plant growth. This study aims to examine the growth of Falcataria moluccana (Miq.) Barneby & J.W.Grimes and Albizia chinensis (Osbeck) Merr seedlings under exposure of aluminum. This study used an one-factor completely randomized design (Al concentration) consisting of 5 levels, namely 0, 2, 4, 6, and 8 mM. Each treatment was repeated 3 times and each replication consisted of 3 plant units. The results showed that the Al exposure treatment gave significant differences in the growth of height, root length, dry weight (root, shoot, and total) of F. moluccana and A. chinensis seedlings. The 2 mM Al concentration stimulated the growth of height, root length and dry weight (root, shoot, and total) of A. chinensis seedlings. The tolerance index for F. moluccana and A. chinensis seedlings was highest when the Al 2 mM concentration was 147.55% and 115.32%, respectively. 2 mM Al exposure treatment increased the chlorophyll content a, b, total chlorophyll and carotenoids of F. moluccana and A. chinensis seedlings. Al exposure treatment did not significantly differ from the rate of photosynthesis and MDA content in F. moluccana and A. chinensis seedlings. The Al content in the roots was higher than in the shoots, and the increase in Al concentration increased the Al content in the roots and shoots of F. moluccana and A. chinensis seedlings.

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