Heavy metal resistance and bioremediation capacity of rhizospheric strain BioRPaz-3 Pseudomonas azotoformans endowed with antifungal activities and multi-abiotic stress tolerance in in vitro trials

Recently, C. auris become one of the most prominent members of the genus Candida. Since its occurrence, several C. auris outbreaks have been reported worldwide. These outbreaks were associated with isolates displaying decreased susceptibility towards fluconazole, the first-line agent for prophylaxis. Fluconazole is the most frequently used antifungal drug to treat bloodstream Candida infections. The physiological effects of acquired antifungal resistance was investigated in this species using fluconazole, posaconazole and voriconazole resistant mutant strains generated by the in vitro microevolution method. Alterations in antifungal susceptibility and cross resistance were determined by the microdilution method, utilizing azoles (fluconazole, voriconazole, posaconazole), echinocandins (caspofungin, micafungin, anidulafungin) and a polyene (amphotericin B). Changes in the abiotic stress tolerance was examined by spotting assay, using osmotic stressors, cell wall perturbants and a membrane detergent. To evaluate the impact of the acquired resistance on sterol biosynthesis, ergosterol composition of all generated mutant strains were examined. A potential relationship between virulence and acquired antifungal resistance was also studied both in vitro and in vivo. Phagocytosis of the generated strains by J774.2 mouse macrophage-like cells was measured and analyzed by flow cytometry. In the murine infection model fungal burden of the triazole evolved strains was determined in spleen, kidney, liver and brain and compared to the fungal burden associated with the initial azole susceptible strain. Significant differences in virulence of the initial and the generated strains was observed suggesting a potential connection between the virulence and antifungal susceptibility of the emerging fungal pathogen, C. auris.

Download Full-text

Bioremediation of lead using bacterial isolates and study their plant relieving effect on wheat under metal contamination

Bhartiya Krishi Anusandhan Patrika ◽

10.18805/bkap222 ◽

2020 ◽

Vol 35 (1-2) ◽

Author(s):

Prashakha J. Shukla ◽

Vishwa R. Vyas

Keyword(s):

Heavy Metal ◽

Stress Condition ◽

Inhibitory Concentration ◽

Anthropogenic Activities ◽

Wheat Plant ◽

Cost Effective ◽

Heavy Metal Resistance ◽

Metal Resistance

Increasing concentration of heavy metals due to various anthropogenic activities is a serious problem. To overcome this issue, many chemical and physical methods are available but they are either directly or indirectly harmful to nature. By these methods more quality of chemicals are wasted. So, bioremediation is the best method to remove pollutants. It is an eco-friendly and cost-effective process. A low concentration of heavy metal is required to plant for their growth and metabolic process but at higher concentration, plants do not survive. With the use of microbes, we can survive plants at certain levels. During this work heavy metal tolerating microorganism was isolated and purified. Various tests were performed like staining, minimum inhibitory concentration, multiple heavy metal resistance, multiple antibiotic resistance, biochemical test, DNA isolation, in vitro examination of the wheat plant under the stress condition of lead (1000ppm).

Download Full-text

Expression of Rice CYP450-Like Gene (Os08g01480) in Arabidopsis Modulates Regulatory Network Leading to Heavy Metal and Other Abiotic Stress Tolerance

PLoS ONE ◽

10.1371/journal.pone.0138574 ◽

2015 ◽

Vol 10 (9) ◽

pp. e0138574 ◽

Cited By ~ 16

Author(s):

Arti Rai ◽

Ruchi Singh ◽

Pramod Arvind Shirke ◽

Rudra Deo Tripathi ◽

Prabodh Kumar Trivedi ◽

...

Keyword(s):

Heavy Metal ◽

Abiotic Stress ◽

Stress Tolerance ◽

Regulatory Network ◽

Abiotic Stress Tolerance

Download Full-text

Analysis of the Genome of the Heavy Metal Resistant and Hydrocarbon-Degrading Rhizospheric Pseudomonas qingdaonensis ZCR6 Strain and Assessment of Its Plant-Growth-Promoting Traits

International Journal of Molecular Sciences ◽

10.3390/ijms23010214 ◽

2021 ◽

Vol 23 (1) ◽

pp. 214

Author(s):

Daria Chlebek ◽

Tomasz Płociniczak ◽

Sara Gobetti ◽

Agata Kumor ◽

Katarzyna Hupert-Kocurek ◽

...

Keyword(s):

Heavy Metal ◽

Plant Growth ◽

Contaminated Soils ◽

Growth Promotion ◽

Sulfur Metabolism ◽

Heavy Metal Resistance ◽

Metal Resistance ◽

Minimal Inhibitory Concentrations ◽

Plant Growth Promoting

The Pseudomonas qingdaonensis ZCR6 strain, isolated from the rhizosphere of Zea mays growing in soil co-contaminated with hydrocarbons and heavy metals, was investigated for its plant growth promotion, hydrocarbon degradation, and heavy metal resistance. In vitro bioassays confirmed all of the abovementioned properties. ZCR6 was able to produce indole acetic acid (IAA), siderophores, and ammonia, solubilized Ca3(PO4)2, and showed surface active properties and activity of cellulase and very high activity of 1-aminocyclopropane-1-carboxylic acid deaminase (297 nmol α-ketobutyrate mg−1 h−1). The strain degraded petroleum hydrocarbons (76.52% of the initial hydrocarbon content was degraded) and was resistant to Cd, Zn, and Cu (minimal inhibitory concentrations reached 5, 15, and 10 mM metal, respectively). The genome of the ZCR6 strain consisted of 5,507,067 bp, and a total of 5055 genes were annotated, of which 4943 were protein-coding sequences. Annotation revealed the presence of genes associated with nitrogen fixation, phosphate solubilization, sulfur metabolism, siderophore biosynthesis and uptake, synthesis of IAA, ethylene modulation, heavy metal resistance, exopolysaccharide biosynthesis, and organic compound degradation. Complete characteristics of the ZCR6 strain showed its potential multiway properties for enhancing the phytoremediation of co-contaminated soils. To our knowledge, this is the first analysis of the biotechnological potential of the species P. qingdaonensis.

Download Full-text

Abiotic stresses induced physiological, biochemical, and molecular changes in Betula platyphylla: a review

Silva Fennica ◽

10.14214/sf.10516 ◽

2021 ◽

Vol 55 (3) ◽

Author(s):

Faujiah Ritonga ◽

Jacob Ngatia ◽

Run Song ◽

Umar Farooq ◽

Sonia Somadona ◽

...

Keyword(s):

Heavy Metal ◽

Transcription Factors ◽

Abiotic Stress ◽

Stress Tolerance ◽

Metal Tolerance ◽

Abiotic Stress Tolerance ◽

Heavy Metal Tolerance ◽

Dry Weight ◽

Betula Platyphylla ◽

Enzymatic Antioxidant

Abiotic stress is one of the major factors in reducing plant growth, development, and yield production by interfering with various physiological, biochemical, and molecular functions. In particular, abiotic stress such as salt, low temperature, heat, drought, UV-radiation, elevated CO2, ozone, and heavy metals stress is the most frequent study in Sukaczev. is one of the most valuable tree species in East Asia facing abiotic stress during its life cycle. Using transgenic plants is a powerful tool to increase the abiotic stress tolerance. Generally, abiotic stress reduces leaves water content, plant height, fresh and dry weight, and enhances shed leaves as well. In the physiological aspect, salt, heavy metal, and osmotic stress disturbs seed germination, stomatal conductance, chlorophyll content, and photosynthesis. In the biochemical aspect, salt, drought, cold, heat, osmotic, UV-B radiation, and heavy metal stress increases the ROS production of cells, resulting in the enhancement of enzymatic antioxidant (SOD and POD) and non-enzymatic antioxidant (proline and AsA) to reduce the ROS accumulation. Meanwhile, upregulates various genes, as well as proteins to participate in abiotic stress tolerance. Based on recent studies, several transcription factors contribute to increasing abiotic stress tolerance in , including , and . These transcription factors bind to different cis-acting elements to upregulate abiotic stress-related genes, resulting in the enhancement of salt, drought, cold, heat, osmotic, UV-B radiation, and heavy metal tolerance. These genes along with phytohormones mitigate the abiotic stress. This review also highlights the candidate genes from another Betulacea family member that might be contributing to increasing abiotic stress tolerance.Betula platyphyllaBetula platyphyllaB. platyphyllaB. platyphyllaB. platyphyllaB. platyphyllaBplMYB46, BpMYB102, BpERF13, BpERF2, BpHOX2, BpHMG6, BpHSP9, BpUVR8, BpBZR1, BplERD15BpNACsB. platyphylla

Download Full-text

Response of the callus cells of fir (Abies nordmanniana) to in vitro heavy metal stress

Folia Forestalia Polonica ◽

10.1515/ffp-2017-0003 ◽

2017 ◽

Vol 59 (1) ◽

pp. 25-33 ◽

Cited By ~ 3

Author(s):

Katarzyna Nawrot-Chorabik

Keyword(s):

Heavy Metals ◽

Heavy Metal ◽

Heavy Metal Stress ◽

Heavy Metal Resistance ◽

Metal Resistance ◽

Stress Factors ◽

Negative Effects ◽

Abies Nordmanniana ◽

Long Time

Abstract The aim of the presented research was to investigate the effect of three heavy metals - lead, cadmium and copper - on the callus cells of Abies nordmanniana. The toxicity degree and toxicity effect of the selected heavy metals was determined on the embryonic level. On the basis of the spectrometric analyses as well as macroscopic and microscopic observations, this research referred to the accumulation of heavy metals in tissues, assuming that this mechanism is related to the acquisition of tolerance by cells exposed to this type of abiotic stress. Moreover, the effect of the genotype of fir on the cell defence, that is, the induction of tolerance, was analysed. Understanding of the issues related to the heavy metal resistance of plant genotypes in future may contribute to the selection of genotypes of individuals that are more resistant to stress factors, particularly in the multi-directional and rational forest management. The results showed that lead (20 mg l-1), which proved to be the most toxic amongst the three examined heavy metals, has the most severe negative effects on the tissue of fir trees. Copper (20 mg l-1) was accumulated for a long time in the cells of fir trees, and it was not degraded or excreted outside the tissues even after three weeks of in vitro culture. Of the three tested genotypes, G14 had the greatest tendency to accumulate each of the examined metals, that is, it appeared to be the least tolerant genotype.

Download Full-text

Rice (Oryza sativa L.) tau class glutathione S-transferase (OsGSTU30) overexpression in Arabidopsis thaliana modulates a regulatory network leading to heavy metal and drought stress tolerance

Metallomics ◽

10.1039/c8mt00204e ◽

2019 ◽

Vol 11 (2) ◽

pp. 375-389 ◽

Cited By ~ 12

Author(s):

Dipali Srivastava ◽

Giti Verma ◽

Abhishek Singh Chauhan ◽

Veena Pande ◽

Debasis Chakrabarty

Keyword(s):

Heavy Metal ◽

Arabidopsis Thaliana ◽

Oryza Sativa ◽

Catalytic Activity ◽

Abiotic Stress ◽

Stress Tolerance ◽

Regulatory Network ◽

Oryza Sativa L ◽

Abiotic Stress Tolerance ◽

Glutathione S Transferase

OsGSTU30 increases the abiotic stress tolerance in plants either by its catalytic activity or by modulating the expression of stress responsive genes.

Download Full-text