Electroporation: High frequency of occurrence of a transient high-permeability state in erythrocytes and intact yeast

FEBS Letters ◽  
1988 ◽  
Vol 229 (1) ◽  
pp. 30-34 ◽  
Author(s):  
James C. Weaver ◽  
Gail I. Harrison ◽  
Jonathan G. Bliss ◽  
Judith R. Mourant ◽  
Kevin T. Powell
2020 ◽  
Vol 62 ◽  
pp. 172-178
Author(s):  
S. E. Golovin

The article highlights the results of research carried out in 2019-2020 to study the reasons for the drying out of the stems of black currant (Ribes nigrum L.), red currant (Ribes rubrum L.) and golden currant (Ribes aureum L.). As a result of research, the fungus Sphaeropsis malorum Peck. in the first in Russia it was revealed from the drying stems of three types of black currants, red currants and golden currants. This  pathogen inhabited currant stems together with fungi from the genus Cytospora spp., causative agents of stem drying. On red currants, it was established that S. malorum was the main causative agent of branch drying, as indicated by the high frequency of occurrence (75.0-83.3 %) of this fungus on the affected stems, compared with species from the genus Cytospora spp. (16.7-33.3 %) and Phomopsis ribesia (25.0 %). Due to the fact that the cultivation of currants in the immediate vicinity of apple and pear trees leads to infection of plants with the fungus S. malorum, it is necessary to avoid planting currants in the vicinity of fruit-bearing seed gardens.


2017 ◽  
Vol 68 (11) ◽  
pp. 2566-2569 ◽  
Author(s):  
Elena Rusu ◽  
Ionela Sarbu ◽  
Magdalena Mitache ◽  
Horatiu Moldovan ◽  
Carmen Ioana Biris ◽  
...  

The high frequency of occurrence of candidiasis as well as high mortality of patients with immunosuppression cause a tendency toward better understanding of Candida albicans species virulence factors and developing sensitive and specific diagnostic methods, and appropriate strategies of candidiasis treatment. In recent decades the incidence of fungal infections has alarming increases because of advanced medical treatments. In this study was analyzed possible ultrastructural changes of the species C. albicans cells following treatment with sodium diclofenac at various concentrations. Following treatment of C. albicans cells with sodium diclofenac 1 mM and 2 mM changes in the plasmalemma can be noticed, changes in the density of cell wall, disruption and necrotic appearance of the cytoplasm.


2021 ◽  
Vol 11 (5) ◽  
pp. 2237
Author(s):  
Oh Heon Kwon ◽  
Won Bin Park ◽  
Juho Yun ◽  
Hong Jun Lim ◽  
Keum Cheol Hwang

In this paper, a low-profile HF (high-frequency) meandered dipole antenna with a ferrite-loaded artificial magnetic conductor (AMC) is proposed. To operate in the HF band while retaining a compact size, ferrite with high permeability is applied to the unit cell of the AMC. The operating frequency bandwidth of the designed unit cell of the AMC is 1.89:1 (19–36 MHz). Thereafter, a meandered dipole antenna is designed by implementing a binary genetic algorithm and is combined with the AMC. The overall size of the designed antenna is 0.06×0.06×0.002 λ3 at the lowest operating frequency. The proposed dipole antenna with a ferrite-loaded AMC is fabricated and measured. The measured VSWR bandwidth (<3) covers 20–30 MHz on the HF band. To confirm the performance of the antenna, a reference monopole antenna which operates on the HF band was selected, and the measured receiving power is compared with the result of the proposed antenna with the AMC.


2013 ◽  
Vol 9 (1) ◽  
pp. 25-28
Author(s):  
Е. Светлакова ◽  
E. Svetlakova ◽  
Ю. Мандра ◽  
J. Mandra ◽  
Н. Жегалина ◽  
...  

<p>Nowadays the treatment of periodontal diseases is an actual problem, that is caused by high frequency of occurrence of periodontopathies. diode dental lasers are nowadays often used for treatment periodontal pockets. Using hydrogel based on silativit and percutaneous electroneurostimulation healing of the wound surfaces.</p><p> </p><p> </p>


2020 ◽  
Vol 32 ◽  
Author(s):  
Tatiane Araujo Santos ◽  
Thamires Souza Gonçalves ◽  
Patricia Silva do Nascimento ◽  
Cézar Augusto Freire Fernandes ◽  
Francisca Edna de Andrade Cunha

Abstract: Aim Identify seasonal variations in the diet of juvenile living in the estuary of the Parnaíba River Delta. Methods Food items were identified through analysis of stomach contents, and later submitted to analysis by regular methods for Frequency of Occurrence (FO), Frequency Numerical (FN), Gravimetric Frequency (FW) and Relative Importance Index (IRI). Results Juvenile diet of Elops saurus that inhabit the lagoons in the Parnaíba delta was composed in order decreasing of importance by Osteichthyes (Teleostei), Insecta and Crustacea in addition to Polychaeta and Nematoda. It was also observed a high frequency of occurrence of fragments of plastics in the diet. In the rainy season, 17 items were recorded in the diet, whereas in the dry season only 7 items were present. Relative Importance Index showed high presence of the Teleostei fish (69.93%) on diet of E. saurus during the rainy season, whereas during the dry period the insects of the families Corixidae (43.25%) and Dysticidae (39.16%). Conclusions With the study it was possible to identify that juvenile are piscivorous in the rainy season when there is a higher availability of prey in the environment and insectivores in the dry season when it reduces the supply of food, indicating feeding flexibility to environmental alterations.


1996 ◽  
Vol 157 ◽  
pp. 207-211
Author(s):  
A. V. Zasov ◽  
O. K. Sil’chenko

AbstractAnalysis of ionized gas velocity fields in the circumnuclear regions of 13 normal spiral galaxies has shown that about half of them reveal a discrepancy between the kinematical axis at R < 2″ and the photometric or kinematical axes of the outer regions. This result indicates a high frequency of occurrence of non-axisymmetric gravitational potentials in the inner few hundred parsecs of galaxies. The possible nature of these minibars is discussed.


Author(s):  
Yu. A. Tyurin ◽  
R. S. Fassakhov ◽  
T. V. Grigorieva ◽  
I. G. Mustafin

Aim. Study transformation of skin microflora during development of atopic dermatitis. Materials and methods. 45 patients with various forms of atopic dermatitis (AtD) were examined. Control group consisted of 26 healthy individuals. The strains were cultivated on elective nutrient media. Identification of the isolated strains was carried out by MALDI-TOF mass-spectrometry method. Results. A low frequency of occurrence of taxon Staphylococcus epidermidis on face skin and high frequency of occurrence of Staphylococcus aureus on upper and lower limb skin was established for AtD patients compared with healthy individuals. The frequency of occurrence of proteolytically active isolates of S. aureus in AtD patients was 3 times higher than in healthy carriers of this taxon. Taxons of microorganisms not inherent to healthy individuals such as Bacillus mycoides, Pseudomonas putida, Pseudomonas radiobacter were isolated on lower limb and neck skin of AtD patients. A high frequency of occurrence of Cryptococcus satoi, Candida albicans, Malassezia globosa fungi was noted. Conclusion. A decrease of barrier functions of skin during AtD facilitates contamination of patients’ skin with rare bacterial taxons and fungi. One of the possible mechanisms of suppression of immune competent cell functions could be proteolytic enzymes of S. aureus.


2021 ◽  
Vol 8 ◽  
Author(s):  
Krista van der Linde ◽  
Ingrid N. Visser ◽  
Rick Bout ◽  
Chris Lalas ◽  
Lara Shepherd ◽  
...  

Leopard seals (Hydrurga leptonyx) are top-order predators that prey on a wide variety of species including crustaceans, cephalopods, fishes, birds, and pinnipeds. While multiple diet studies have been conducted worldwide, there are no previous accounts of leopard seals predating on chondrichthyans. As part of a wider study on the diet of leopard seals in New Zealand (NZ) waters, researchers and citizen scientists recorded 39 observations of predation and collected 127 scats (166 total records) between 1942 and 2019. Predation on chondrichthyans was detected in 23.1% (n = 9) of observations of predation and 7.1% (n = 9) of scats (the latter via morphological examination and DNA sequencing). From both observations of predations and scats, three chondrichthyan species or genus were identified; elephantfish (Callorhinchus milii), ghost sharks (Hydrolagus spp.) and spiny dogfish (Squalus acanthias). While this is the first published record of leopard seals feeding on chondrichthyans, the relatively high frequency of occurrence within our NZ records, and that certain individuals appeared to target this type of prey, indicates that these species could constitute a substantial, or important, part of the diet for some leopard seals in this region. As chondrichthyans form an important part of the NZ marine ecosystems, our recognition of an additional top-order predator of these species contributes to understanding the overall health of, and future impacts of predators on, the wider NZ marine ecosystem.


2018 ◽  
Vol 5 (1) ◽  
pp. 1-5 ◽  
Author(s):  
João Pedro Souza-Alves ◽  
Natasha M. Albuquerque ◽  
Luana Vinhas ◽  
Thayane S. Cardoso ◽  
Raone Beltrão-Mendes ◽  
...  

Abstract. Self-anointing behaviour using Bauhinia sp. was reported in two captive titi monkeys (Callicebus coimbrai and Callicebus barbarabrownae). The study was carried out from October 2013 to May 2014 during an experimental study investigating the gut passage time of these individuals at the Getúlio Vargas Zoobotanical Park, north-eastern Brazil. Although leaves, petioles and flowers of Bauhinia contain chemical substances that could affect the presence of ectoparasites, it is unclear if titi monkeys demonstrate self-anointing behaviour as a method of self-medication. However, due to the presence of large glands in C. coimbrai and C. barbarabrownae chests, and the high frequency of occurrence observed for the adult male, we cautiously suggest that the use of Bauhinia may be linked to olfactory communication.


Author(s):  
Massimiliano Burlando ◽  
Djordje Romanic

Severe winds produced by thunderstorm outflows cause damage to structures and properties worldwide. Due to their high frequency of occurrence, these winds can be considered one of the most high-risk phenomena in the plethora of the dangerous manifestations of the weather in the troposphere. Through the analysis of experimental data that initiated with visual observations and followed with quantitative measurements, the downburst phenomenon was first discovered and measured in the second half of the last century. Since then, the physical processes responsible for downburst formation have been identified thanks to the advances in observing systems, numerical modeling techniques, as well as smaller scale physical experiments carried out under well-controlled and confined conditions. The purpose of this chapter is to summarize what has been learned about kinematics, dynamics, and thermodynamics of downbursts and unveil the physics behind this phenomenon, running through the most relevant discoveries over the last half century in this research field.


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