Laser-assisted NMR in the Presence of a Cryogenic Probe Enables Multidimensional Data Collection on Amino Acids and Proteins at Unprecedented Sensitivity

Most of the current health management products are used in medical institutions and generally do not pay enough attention to the student population. Based on this, this paper designs a student-oriented and functional autonomous health management system. This paper proposes a personal health management system based on a multidimensional data model based on the main social characteristics of the population with chronic diseases and the actual needs of personal health management for chronic diseases. The value of various health data for health management is deeply analyzed and mined, and a multidimensional model data warehouse is constructed according to relevant national health data standards to create a standard data platform for intelligent health warning and disease risk assessment. This paper researches and designs a closed-loop personal health management method based on the Plan-Do-Check-Action (PDCA) cycle management model, with detailed functional design in four aspects: health data collection and recording, health assessment, health planning, and tracking and execution. This paper researches health data collection, processing, and storage technologies and adopts HDFS data storage technology, html, css, Java Script, java, and other software development technologies, combined with j Query, UEditor, Date Range Picker, and other plug-ins, as well as SMS email generation interface, wireless Bluetooth transmission interface, etc. This system web and mobile application platforms are designed and developed. Relational database is used as the system database, and a snowflake-type multidimensional data model is designed. Finally, the functions and performance of this system were tested, and the development and trial run of the basic version have been completed.

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Be Jubel Dan Be Ancruk Kuliner Ekstrim Langka Di Bali

Journey : Journal of Tourismpreneurship, Culinary, Hospitality, Convention and Event Management ◽

10.46837/journey.v1i1.16 ◽

2019 ◽

Vol 1 (1 Special Edition) ◽

pp. 42-61

Keyword(s):

Amino Acids ◽

Data Collection ◽

Farming System ◽

Essential Amino Acids ◽

Body Tissues ◽

Insect Larva ◽

Green Zone ◽

Organic Farming System ◽

Research Show

Jubel is a caterpillar-shaped water insect larva that lives in muddy waters. Ancruk, in the form of a white elongated caterpillar, is known as a sago caterpillar. This article aims to (1) describe gastronomic beams and features. (2) obtain a strategy of avoiding the extinction of the living resources of jubel and ancruk. The research used gastronomic theory and the theory of revitalization. The data collection techniques used are interviews and literature. The results of the research show that be jubel and ancruk have good savor for the human body. They are of high protein (supports growth and maintenance of body tissues), free of cholesterol, contain essential amino acids, vitamins, minerals, and can maintain stamina and low fat. The strategy that can be done is to revitalize the living sources of the main ingredients, such as physical intervention by limiting development in the green zone. Improving the quality of existing rice fields, implementing an organic farming system. Meanwhile, to save ancruk is to maintain the existence of their plants habitat and to do cultivation. Keywords: Jubel, Ancruk, Rare, Bali

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Homochirality as the Signature of Extra-Terrestrial Life

International Astronomical Union Colloquium ◽

10.1017/s0252921100015037 ◽

1997 ◽

Vol 161 ◽

pp. 505-510

Author(s):

Alexandra J. MacDermott ◽

Laurence D. Barron ◽

Andrè Brack ◽

Thomas Buhse ◽

John R. Cronin ◽

...

Keyword(s):

Amino Acids ◽

Protein Synthesis ◽

Optical Rotation ◽

Physical Origin ◽

Natural Choice ◽

Rosetta Mission ◽

Terrestrial Life ◽

Subsurface Layers ◽

Solar System Bodies ◽

Origin Of Homochirality

AbstractThe most characteristic hallmark of life is its homochirality: all biomolecules are usually of one hand, e.g. on Earth life uses only L-amino acids for protein synthesis and not their D mirror images. We therefore suggest that a search for extra-terrestrial life can be approached as a Search for Extra- Terrestrial Homochirality (SETH). The natural choice for a SETH instrument is optical rotation, and we describe a novel miniaturized space polarimeter, called the SETH Cigar, which could be used to detect optical rotation as the homochiral signature of life on other planets. Moving parts are avoided by replacing the normal rotating polarizer by multiple fixed polarizers at different angles as in the eye of the bee. We believe that homochirality may be found in the subsurface layers on Mars as a relic of extinct life, and on other solar system bodies as a sign of advanced pre-biotic chemistry. We discuss the chiral GC-MS planned for the Roland lander of the Rosetta mission to a comet and conclude with theories of the physical origin of homochirality.

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Hydrogen Cyanide Polymers from the Impact of Comet P/Shoemaker-Levy 9 on Jupiter

International Astronomical Union Colloquium ◽

10.1017/s025292110001469x ◽

1997 ◽

Vol 161 ◽

pp. 179-187

Author(s):

Clifford N. Matthews ◽

Rose A. Pesce-Rodriguez ◽

Shirley A. Liebman

Keyword(s):

Amino Acids ◽

Solar System ◽

Hydrogen Cyanide ◽

Nitrogen Heterocycles ◽

Laboratory Studies ◽

Heterogeneous Solids ◽

The Many ◽

Comet Halley ◽

The Impact ◽

By Products

AbstractHydrogen cyanide polymers – heterogeneous solids ranging in color from yellow to orange to brown to black – may be among the organic macromolecules most readily formed within the Solar System. The non-volatile black crust of comet Halley, for example, as well as the extensive orangebrown streaks in the atmosphere of Jupiter, might consist largely of such polymers synthesized from HCN formed by photolysis of methane and ammonia, the color observed depending on the concentration of HCN involved. Laboratory studies of these ubiquitous compounds point to the presence of polyamidine structures synthesized directly from hydrogen cyanide. These would be converted by water to polypeptides which can be further hydrolyzed to α-amino acids. Black polymers and multimers with conjugated ladder structures derived from HCN could also be formed and might well be the source of the many nitrogen heterocycles, adenine included, observed after pyrolysis. The dark brown color arising from the impacts of comet P/Shoemaker-Levy 9 on Jupiter might therefore be mainly caused by the presence of HCN polymers, whether originally present, deposited by the impactor or synthesized directly from HCN. Spectroscopic detection of these predicted macromolecules and their hydrolytic and pyrolytic by-products would strengthen significantly the hypothesis that cyanide polymerization is a preferred pathway for prebiotic and extraterrestrial chemistry.

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Electron Diffraction of Wet Biological Membranes

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100050809 ◽

1974 ◽

Vol 32 ◽

pp. 158-159

Author(s):

S.W. Hui ◽

D.F. Parsons

Keyword(s):

Electron Diffraction ◽

Data Collection ◽

Biological Membranes ◽

Small Areas ◽

Electron Diffraction Technique ◽

Electron Microscopes ◽

Collection Time ◽

Camera Length

The development of the hydration stages for electron microscopes has opened up the application of electron diffraction in the study of biological membranes. Membrane specimen can now be observed without the artifacts introduced during drying, fixation and staining. The advantages of the electron diffraction technique, such as the abilities to observe small areas and thin specimens, to image and to screen impurities, to vary the camera length, and to reduce data collection time are fully utilized. Here we report our pioneering work in this area.

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Methionine-Specific Staining of Collagen

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s042482010005408x ◽

1982 ◽

Vol 40 ◽

pp. 294-295

Author(s):

E.M. Kuhn ◽

K.D. Marenus ◽

M. Beer

Keyword(s):

Amino Acids ◽

Collagen Fibers ◽

Type Iii Collagen ◽

Type I ◽

Electron Microscopic ◽

Good Correspondence ◽

Specific Staining ◽

Type Iii ◽

Different Types ◽

Sulfur Containing

Fibers composed of different types of collagen cannot be differentiated by conventional electron microscopic stains. We are developing staining procedures aimed at identifying collagen fibers of different types.Pt(Gly-L-Met)Cl binds specifically to sulfur-containing amino acids. Different collagens have methionine (met) residues at somewhat different positions. A good correspondence has been reported between known met positions and Pt(GLM) bands in rat Type I SLS (collagen aggregates in which molecules lie adjacent to each other in exact register). We have confirmed this relationship in Type III collagen SLS (Fig. 1).

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Automated electron tomography: from data collection to image processing

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100136507 ◽

1995 ◽

Vol 53 ◽

pp. 26-27

Author(s):

Weiping Liu ◽

Jennifer Fung ◽

W.J. de Ruijter ◽

Hans Chen ◽

John W. Sedat ◽

...

Keyword(s):

Image Processing ◽

Data Collection ◽

Structural Information ◽

Imaging Technique ◽

Electron Tomography ◽

Ccd Camera ◽

Automated Data Collection ◽

Full Control ◽

Transmission Electron ◽

Amorphous Structures

Electron tomography is a technique where many projections of an object are collected from the transmission electron microscope (TEM), and are then used to reconstruct the object in its entirety, allowing internal structure to be viewed. As vital as is the 3-D structural information and with no other 3-D imaging technique to compete in its resolution range, electron tomography of amorphous structures has been exercised only sporadically over the last ten years. Its general lack of popularity can be attributed to the tediousness of the entire process starting from the data collection, image processing for reconstruction, and extending to the 3-D image analysis. We have been investing effort to automate all aspects of electron tomography. Our systems of data collection and tomographic image processing will be briefly described.To date, we have developed a second generation automated data collection system based on an SGI workstation (Fig. 1) (The previous version used a micro VAX). The computer takes full control of the microscope operations with its graphical menu driven environment. This is made possible by the direct digital recording of images using the CCD camera.

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Prevalence of the pit and antipit in the genus Glycine

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100129218 ◽

1987 ◽

Vol 45 ◽

pp. 986-987

Author(s):

R. W. Yaklich ◽

E. L. Vigil ◽

W. P. Wergin

Keyword(s):

Amino Acids ◽

Endoplasmic Reticulum ◽

Glycine Max ◽

Seed Coat ◽

Cell Types ◽

Abaxial Surface ◽

Legume Seed ◽

Initial Report ◽

Cone Cells ◽

Glycine Max L

The legume seed coat is the site of sucrose unloading and the metabolism of imported ureides and synthesis of amino acids for the developing embryo. The cell types directly responsible for these functions in the seed coat are not known. We recently described a convex layer of tissue on the inside surface of the soybean (Glycine max L. Merr.) seed coat that was termed “antipit” because it was in direct opposition to the concave pit on the abaxial surface of the cotyledon. Cone cells of the antipit contained numerous hypertrophied Golgi apparatus and laminated rough endoplasmic reticulum common to actively secreting cells. The initial report by Dzikowski (1936) described the morphology of the pit and antipit in G. max and found these structures in only 68 of the 169 seed accessions examined.

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Imaging polymers, proteins and dna in aqueous solutions with the atomic force microscope

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100152136 ◽

1989 ◽

Vol 47 ◽

pp. 32-33

Author(s):

S.A.C. Gould ◽

B. Drake ◽

C.B. Prater ◽

A.L. Weisenhorn ◽

S.M. Lindsay ◽

...

Keyword(s):

Amino Acids ◽

Dna Sequencing ◽

Aqueous Solutions ◽

Atomic Force Microscope ◽

Piezoelectric Crystal ◽

Atomic Force ◽

Structure Of Molecules ◽

Optical Lever

The atomic force microscope (AFM) is an instrument that can be used to image many samples of interest in biology and medicine. Images of polymerized amino acids, polyalanine and polyphenylalanine demonstrate the potential of the AFM for revealing the structure of molecules. Images of the protein fibrinogen which agree with TEM images demonstrate that the AFM can provide topographical data on larger molecules. Finally, images of DNA suggest the AFM may soon provide an easier and faster technique for DNA sequencing.The AFM consists of a microfabricated SiO2 triangular shaped cantilever with a diamond tip affixed at the elbow to act as a probe. The sample is mounted on a electronically driven piezoelectric crystal. It is then placed in contact with the tip and scanned. The topography of the surface causes minute deflections in the 100 μm long cantilever which are detected using an optical lever.

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