18. Two distinct cell lines in one hepatoblastoma tissue block

2020 ◽  
Vol 244 ◽  
pp. 7
Author(s):  
Leslie Grimmett ◽  
Joanna Plunkitt ◽  
Tamara Restrepo ◽  
Alanna Church ◽  
Sanda Alexandrescu ◽  
...  
Keyword(s):  
Cell Lines ◽  
Tissue Block ◽  
Distinct Cell

scholarly journals Modulation of Pathways Underlying Distinct Cell Death Mechanisms in Two Human Lung Cancer Cell Lines in Response to SN1 Methylating Agents Treatment

PLoS ONE ◽  
2016 ◽  
Vol 11 (7) ◽  
pp. e0160248 ◽  
Author(s):  
Olga Papadodima ◽  
Panagiotis Moulos ◽  
Aggeliki Koryllou ◽  
Georgia Piroti ◽  
Fragiskos Kolisis ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Cell Death ◽  
Cell Lines ◽  
Cancer Cell ◽  
Human Lung ◽  
Human Lung Cancer ◽  
Lung Cancer Cell ◽  
Distinct Cell ◽  
Cell Death Mechanisms ◽  
Methylating Agents

scholarly journals A Radioautographic Study of Hemopoietic Repopulation Using Irradiated Parabiotic Rats

Blood ◽  
1966 ◽  
Vol 28 (6) ◽  
pp. 873-890 ◽  
Author(s):  
RUTH W. CAFFREY TYLER ◽  
N. B. EVERETT
Keyword(s):  
Bone Marrow ◽  
Stem Cell ◽  
Lymph Nodes ◽  
Cell Lines ◽  
Cell Types ◽  
Cell Type ◽  
Distinct Cell ◽  
Major Cell Type

Abstract These radioautographic studies using parabiotic rats and partial marrow shielding showed that cells responsible for recovery of irradiated bone marrow had their origin in the shielded marrow. Three morphologically distinct cell types appeared in the blood of these parabionts, mature granulocytes, small lymphocytes and monocytoid cells. The monocytoid was the major cell type which crossed from the shielded to nonshielded marrow, and the observations suggested that it is this cell which served as a stem cell for both the erythrocytic and granulocytic cell lines. Labeled erythroblasts and myeloblasts were observed in the recovering marrow, and the labeling intensity of these cells indicated that they were the second or third division products of labeled immigrant cells. The effect of marrow shielding upon the recovery of lymphopoiesis in spleen, thymus, lymph nodes and bone marrow is also discussed.

scholarly journals Acquiring Metastatic Competence by Oral Squamous Cell Carcinoma Cells Is Associated with Differential Expression ofα-Tubulin Isoforms

2012 ◽  
Vol 2012 ◽  
pp. 1-9 ◽  
Author(s):  
Becky Lou ◽  
David Engler ◽  
William Dubinsky ◽  
Jean Wu ◽  
Nadarajah Vigneswaran
Keyword(s):  
Cell Lines ◽  
Cell Shape ◽  
Quantitative Difference ◽  
Distribution Patterns ◽  
Carcinoma Cells ◽  
Protein Levels ◽  
Distinct Cell ◽  
Tubulin Isoforms

We performed comparative global proteomics analyses of patient-matched primary (686Tu) and metastatic (686Ln) OSCC cells. The metastatic OSCC 686Ln cells showed greaterin vitromigratory/invasive potential and distinct cell shape from their parental primary 686Tu cells. Ettan DIGE analysis revealed 1316 proteins spots in both cell lines with >85% to be quantitatively similar (<2 folds) between the two cell lines. However, two protein spots among four serial spots were highly dominant in 686Ln cells. Mass spectrometry sequencing demonstrated all four spots to beα-tubulin isotypes. Further analysis showed no significant quantitative difference in theα-tubulin between the two cell lines either at mRNA or protein levels. Thus, two distinct isoforms ofα-tubulin, probably due to posttranslational modification, were associated with metastatic 686Ln cells. Immunofluorescence demonstrated remarkable differences in the cytosolicα-tubulin distribution patterns between the two cells. In 686Tu cells,α-tubulin proteins formed a normal network composed of filaments. In contrast,α-tubulin in 686Ln cells exhibited only partial cytoskeletal distribution with the majority of the protein diffusely distributed within the cytosol. Sinceα-tubulin is critical for cell shape and mobility, our finding suggests a role ofα-tubulin isoforms in acquisition of metastatic phenotype and represents potential target for therapeutic intervention.

scholarly journals CellTrans: An R Package to Quantify Stochastic Cell State Transitions

2017 ◽  
Vol 11 ◽  
pp. 117793221771224 ◽  
Author(s):  
Thomas Buder ◽  
Andreas Deutsch ◽  
Michael Seifert ◽  
Anja Voss-Böhme
Keyword(s):  
Cell Lines ◽  
Transition Probabilities ◽  
Cell Lineage ◽  
R Package ◽  
State Transitions ◽  
Cell State ◽  
Current State ◽  
Cancerous Cell ◽  
Distinct Cell ◽  
A Cell

Many normal and cancerous cell lines exhibit a stable composition of cells in distinct states which can, e.g., be defined on the basis of cell surface markers. There is evidence that such an equilibrium is associated with stochastic transitions between distinct states. Quantifying these transitions has the potential to better understand cell lineage compositions. We introduce CellTrans, an R package to quantify stochastic cell state transitions from cell state proportion data from fluorescence-activated cell sorting and flow cytometry experiments. The R package is based on a mathematical model in which cell state alterations occur due to stochastic transitions between distinct cell states whose rates only depend on the current state of a cell. CellTrans is an automated tool for estimating the underlying transition probabilities from appropriately prepared data. We point out potential analytical challenges in the quantification of these cell transitions and explain how CellTrans handles them. The applicability of CellTrans is demonstrated on publicly available data on the evolution of cell state compositions in cancer cell lines. We show that CellTrans can be used to (1) infer the transition probabilities between different cell states, (2) predict cell line compositions at a certain time, (3) predict equilibrium cell state compositions, and (4) estimate the time needed to reach this equilibrium. We provide an implementation of CellTrans in R, freely available via GitHub ( https://github.com/tbuder/CellTrans ).

Concurrent Production of Adrenocorticotropin and Prolactin from Two Distinct Cell Lines in a Single Pituitary Adenoma: A Detailed Immunohistochemical Analysis

1982 ◽  
Vol 55 (5) ◽  
pp. 947-955 ◽  
Author(s):  
STEPHEN H. SHERRY ◽  
ANDRÉ T. GUAY ◽  
ARTHUR K. LEE ◽  
E. TESSA HEDLEY-WHYTE ◽  
MICHELINE FEDERMAN ◽  
...  
Keyword(s):  
Pituitary Adenoma ◽  
Cell Lines ◽  
Immunohistochemical Analysis ◽  
Distinct Cell

Chimeric Dizygotic Twins; Twin Study Summaries; News At Home and Abroad

2005 ◽  
Vol 8 (2) ◽  
pp. 178-181 ◽  
Author(s):  
Nancy L. Segal
Keyword(s):  
Cell Lines ◽  
Twin Study ◽  
Disease Susceptibility ◽  
Physical Development ◽  
Twin Research ◽  
Dizygotic Twins ◽  
Separate Cell ◽  
Distinct Cell ◽  
The Military ◽  
At Home

AbstractChimeras, individuals whose genetically distinct cell lines originated from different zygotes, are attracting attention among twin researchers. This is because chimerism may occur more frequently than previously suspected, having been observed among 8% of dizygotic (DZ) twins and 21% of DZ triplets. The presence of separate cell lines in these twins may hold clues to disease susceptibility, developmental complications and histocompatibility features. Next, new twin research on physical development is offering fresh insights on height and hormones, height and education, and pelvic development. Finally, twins having twins, twins deserting the military and twins composing music raise new thoughts about reproduction, behavior and talent.

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