Prevalence and characterization of methicillin-resistant Staphylococcus pseudintermedius from symptomatic companion animals in Northern Italy: Clonal diversity and novel sequence types

2019 ◽  
Vol 66 ◽  
pp. 101331 ◽  
Author(s):  
Maria Luisa Menandro ◽  
Giorgia Dotto ◽  
Alessandra Mondin ◽  
Marco Martini ◽  
Letizia Ceglie ◽  
...  
Keyword(s):  
Companion Animals ◽  
Clonal Diversity ◽  
Northern Italy ◽  
Staphylococcus Pseudintermedius ◽  
Methicillin Resistant ◽  
Sequence Types

scholarly journals Clonal Diversity and Antimicrobial Resistance of Methicillin-Resistant Staphylococcus pseudintermedius Isolated from Canine Pyoderma

2021 ◽  
Vol 9 (3) ◽  
pp. 482
Author(s):  
Vanessa Silva ◽  
Ana Oliveira ◽  
Vera Manageiro ◽  
Manuela Caniça ◽  
Diogo Contente ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Antimicrobial Agents ◽  
Animal Health ◽  
Clonal Diversity ◽  
Multidrug Resistant ◽  
Diffusion Method ◽  
Staphylococcus Pseudintermedius ◽  
Methicillin Resistant ◽  
Genetic Lineages ◽  
Sequence Types

The emergence of methicillin-resistant Staphylococcus pseudintermedius (MRSP) antimicrobial resistance and epidemic genetic lineages is posing a challenge in veterinary medicine due to the limited therapeutical options. MRSP has been identified as an important canine pyoderma pathogen. Thus, we aimed to characterize the antimicrobial resistance and clonal lineages of MRSP isolated from canine cutaneous pyoderma. Thirty-one MRSP isolates recovered from pyoderma were further characterized. The antimicrobial susceptibility testing of the isolates was performed by the Kirby-Bauer disc diffusion method against 14 antimicrobial agents. The presence of antimicrobial and virulence genes was carried out by PCR. Multilocus sequence typing was performed in all isolates. All strains had a multidrug-resistant profile showing resistance mainly to penicillin, macrolides and lincosamides, aminoglycosides, tetracycline and trimethoprim-sulfamethoxazole, which was encoded by the blaZ, ermB, msr(A/B), aac(6′)-Ie-aph(2′′)-Ia, aph(3′)-IIIa, ant(4′)-Ia, tetM, tetK and dfrG genes. All isolates harbored the lukS-I/lukF-I virulence factors. Isolates were ascribed to nine previously described sequence types (STs): ST123, ST339, ST727, ST71, ST537, ST45, ST1029, ST118 and ST1468; and to five STs first described in this study: ST2024, ST2025, ST2026, ST2027 and ST2028. In this study, most isolates belonged to ST123 (n = 16), which belongs to CC71 and is the most common clone in Europe. All isolates were multidrug-resistant, which may impose a serious threat to animal health.

scholarly journals Occurrence and Molecular Characteristics of Methicillin-Resistant Staphylococcus aureus and Methicillin-Resistant Staphylococcus pseudintermedius in an Academic Veterinary Hospital

2010 ◽  
Vol 76 (15) ◽  
pp. 5165-5174 ◽  
Author(s):  
Kanako Ishihara ◽  
Natsumi Shimokubo ◽  
Akie Sakagami ◽  
Hiroshi Ueno ◽  
Yasukazu Muramatsu ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Control Strategies ◽  
Companion Animals ◽  
Molecular Characteristics ◽  
Stepwise Logistic Regression ◽  
Type Ii ◽  
Staphylococcus Pseudintermedius ◽  
Methicillin Resistant ◽  
Type Iv ◽  
Veterinary Hospital

ABSTRACT Recently, methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-resistant Staphylococcus pseudintermedius (MRSP) have been increasingly isolated from veterinarians and companion animals. With a view to preventing the spread of MRSA and MRSP, we evaluated the occurrence and molecular characteristics of each in a veterinary college. MRSA and MRSP were isolated from nasal samples from veterinarians, staff members, and veterinary students affiliated with a veterinary hospital. Using stepwise logistic regression, we identified two factors associated with MRSA carriage: (i) contact with an identified animal MRSA case (odds ratio [OR], 6.9; 95% confidence interval [95% CI], 2.2 to 21.6) and (ii) being an employee (OR, 6.2; 95% CI, 2.0 to 19.4). The majority of MRSA isolates obtained from individuals affiliated with the veterinary hospital and dog patients harbored spa type t002 and a type II staphylococcal cassette chromosome mec (SCCmec), similar to the hospital-acquired MRSA isolates in Japan. MRSA isolates harboring spa type t008 and a type IV SCCmec were obtained from one veterinarian on three different sampling occasions and also from dog patients. MRSA carriers can also be a source of MRSA infection in animals. The majority of MRSP isolates (85.2%) carried hybrid SCCmec type II-III, and almost all the remaining MRSP isolates (11.1%) carried SCCmec type V. MRSA and MRSP were also isolated from environmental samples collected from the veterinary hospital (5.1% and 6.4%, respectively). The application of certain disinfection procedures is important for the prevention of nosocomial infection, and MRSA and MRSP infection control strategies should be adopted in veterinary medical practice.

scholarly journals Characterization of a CC1153 PVL-producing community-acquired methicillin-resistant Staphylococcus aureus from a dog bite wound

2017 ◽  
Vol 11 (06) ◽  
pp. 513-516 ◽  
Author(s):  
Edet Ekpenyong Udo ◽  
Samar Saed Boswihi ◽  
Tsonyu Ivanov Dimitrov ◽  
Bobby Noronha ◽  
Bindu Mathew ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Companion Animals ◽  
Dog Bite ◽  
Bite Wound ◽  
Methicillin Resistant ◽  
Wound Culture ◽  
Genes Encoding ◽  
Disease Hospital

The isolation of a rare community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) strain from an infected dog bite wound is here reported. A 27–year old man presented with a deep open wound on his right hand caused by his dog’s bite at the outpatient clinic of Infectious Disease Hospital (IDH), Kuwait. A wound swab was obtained for bacteriological culture and susceptibility testing. The wound culture yielded pure heavy growth of an MRSA isolate, designated IDH70, which was susceptible to vancomycin, teicoplanin, erythromycin, clindamycin, trimethoprim, fusidic acid and rifampicin. The patient was successfully treated with a combination of rifampicin and cotrimoxazole twice daily for 10 days. Molecular characterization revealed that IDH70 was positive for genes encoding Panton-Valentine leucocidin. IDH70 also carried the SCCmec V genetic element, belonged to coagulase type XIIIa, spa type t903, and was assigned to clonal complex 1153 and sequence type ST1153 (ST1153-V-t903). The report highlights the increasing burden of CA-MRSA in the community and the risk of its acquisition from bites of companion animals.

scholarly journals Characterization of a Novel Composite Staphylococcal Cassette Chromosomemecin Methicillin-Resistant Staphylococcus pseudintermedius from Thailand

2015 ◽  
Vol 60 (2) ◽  
pp. 1153-1157 ◽  
Author(s):  
Pattrarat Chanchaithong ◽  
Nuvee Prapasarakul ◽  
Vincent Perreten ◽  
Sybille Schwendener
Keyword(s):  
Integration Site ◽  
Heavy Metal Resistance ◽  
Metal Resistance ◽  
New Combination ◽  
Staphylococcus Pseudintermedius ◽  
Methicillin Resistant ◽  
Content Type ◽  
Class A ◽  
Restriction Modification

ABSTRACTA novel staphylococcal cassette chromosomemec(SCCmec) composite island (SCCmecAI16-SCCczrAI16-CI) was identified inStaphylococcus pseudintermedius. Four integration site sequences for SCC subdivided the 60,734-bp island into 41,232-bp SCCmecAI16, 19,400-bp SCCczrAI16, and 102-bp SCC-likeAI16elements. SCCmecAI16represents a new combination ofccrA1B3genes with a class Ameccomplex. SCCczrAI16containsccrA1B6and genes related to restriction modification and heavy metal resistance. SCCmecAI16-SCCczrAI16-CI was found in methicillin-resistantS. pseudintermediussequence type 112 (ST112) and ST111 isolated from dogs and veterinarians in Thailand.

scholarly journals Characterization of Staphylococcus pseudintermedius isolated from diseased dogs in Lithuania

2016 ◽  
Vol 19 (1) ◽  
pp. 7-14 ◽  
Author(s):  
M. Ruzauskas ◽  
N. Couto ◽  
A. Pavilonis ◽  
I. Klimiene ◽  
R. Siugzdiniene ◽  
...  
Keyword(s):  
Companion Animals ◽  
Penicillin G ◽  
Special Focus ◽  
Reproductive Disorders ◽  
Gene Encoding ◽  
Staphylococcus Pseudintermedius ◽  
Genes Encoding ◽  
Species Specific ◽  
Staphylococcus Spp

AbstractThe aim of this study was to characterize Staphylococcus pseudintermedius for its antimicrobial resistance and virulence factors with a special focus on methicillin-resistant (MRSP) strains isolated from sick dogs in Lithuania. Clinically sick adult dogs suffering from infections (n=214) and bitches with reproductive disorders (n=36) from kennels were selected for the study. Samples (n=192) from the 250 tested (76.8%) dogs were positive for Staphylococcus spp. Molecular profiling using the species-specific nuc gene identified 51 isolates as S. pseudintermedius (26.6% from a total number of isolated staphylococci) of which 15 isolates were identified as MRSP. Ten MRSP isolates were isolated from bitches with reproductive disorders from two large breeding kennels. Data on susceptibility of S. pseudintermedius to different antimicrobials revealed that all isolates were susceptible to vancomycin, daptomycin and linezolid. Two isolates (3.9%) were resistant to rifampicin. A high resistance was seen towards penicillin G (94.1%), tetracycline (64.7%) and macrolides (68.7%). Resistance to fluoroquinolones ranged from 25.5% (gatifloxacin) to 31.4% (ciprofloxacin). The most prevalent genes encoding resistance included blaZ, aac(6’)-Ie-aph(2’’)-Ia, mecA, and tet(M). The Luk-I gene encoding a leukotoxin was detected in 29% of the isolates, whereas the siet gene encoding exfoliative toxin was detected in 69% of the S. pseudintermedius isolates. This report of MRSP in companion animals represents a major challenge for veterinarians in terms of antibiotic therapy and is a concern for both animal and public health.

scholarly journals Genomic insights into methicillin-resistant Staphylococcus pseudintermedius isolates from dogs and humans of the same sequence types reveals diversity in prophages and pathogenicity islands

PLoS ONE ◽  
2021 ◽  
Vol 16 (7) ◽  
pp. e0254382
Author(s):  
Nathita Phumthanakorn ◽  
Sybille Schwendener ◽  
Valentina Donà ◽  
Pattrarat Chanchaithong ◽  
Vincent Perreten ◽  
...  
Keyword(s):  
Genomic Diversity ◽  
Nucleotide Polymorphisms ◽  
Clonal Lineage ◽  
Genome Sequences ◽  
Single Nucleotide ◽  
Staphylococcus Pseudintermedius ◽  
Methicillin Resistant ◽  
Opportunistic Pathogenic ◽  
High Level ◽  
Sequence Types

Methicillin-resistant Staphylococcus pseudintermedius (MRSP) is an important opportunistic pathogenic bacterium of dogs that also occasionally colonize and infect humans. However, whether MRSP can adapt to human hosts is not clear and whole genome sequences of MRSP from humans are still limited. Genomic comparative analyses of 3 couples of isolates from dogs (n = 3) and humans (n = 3) belonging to ST45, ST112, and ST181, the dominant clones in Thailand were conducted to determine the degree of similarities between human and animal MRSP of a same ST. Among eight prophages, three prophages associated with the leucocidins genes (lukF/S-I), φVB88-Pro1, φVB16-Pro1 and φAP20-Pro1, were distributed in the human MRSPs, while their remnants, φAH18-Pro1, were located in the dog MRSPs. A novel composite pathogenicity island, named SpPI-181, containing two integrase genes was identified in the ST181 isolates. The distribution of the integrase genes of the eight prophages and SpPI-181 was also analysed by PCR in 77 additional MRSP isolates belonging to different STs. The PCR screen revealed diversity in prophage carriage, especially in ST45 isolates. Prophage φAK9-Pro1 was only observed in ST112 isolates from dogs and SpPI-181 was found associated with ST181 clonal lineage. Among the 3 couple of isolates, ST45 strains showed the highest number of single nucleotide polymorphisms (SNP) in their core genomes (3,612 SNPs). The genomic diversity of ST45 isolates suggested a high level of adaptation that may lead to different host colonization of successful clones. This finding provided data on the genomic differences of MRSP associated with colonization and adaption to different hosts.

scholarly journals Molecular Epidemiology of Clinical and Colonizing Methicillin-Resistant Staphylococcus Isolates in Companion Animals

2021 ◽  
Vol 8 ◽  
Author(s):  
Hester Rynhoud ◽  
Brian M. Forde ◽  
Scott A. Beatson ◽  
Sam Abraham ◽  
Erika Meler ◽  
...  
Keyword(s):  
Molecular Epidemiology ◽  
Companion Animals ◽  
High Volume ◽  
Multidrug Resistant ◽  
Clinical Isolates ◽  
Contributing Factors ◽  
Diagnostic Laboratory ◽  
Methicillin Resistant ◽  
The Individual ◽  
Sequence Types

In this study, we aimed to investigate the molecular epidemiology of methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-resistant Staphylococcus pseudintermedius (MRSP) clinical and colonizing isolates of dogs and cats to profile contributing factors associated with their isolation. Nasal and rectal samples were collected from dogs and cats between 2015 and 2017 to identify colonizing isolates. Clinical isolates collected between 2003 and 2016 were retrieved from a Queensland university veterinary diagnostic laboratory. All isolates were identified using standard microbiological and molecular methods and were characterized by whole genome sequencing. Phylogenetic relationships and differences in epidemiological factors were investigated. Seventy-two MRSP isolates out of 1,460 colonizing samples and nine MRSP clinical isolates were identified. No MRSA was isolated. ST496 and ST749 were the most commonly isolated sequence types with different SCCmec types. ST496 clones spread both along the coast and more inland where ST749 was more centered in Brisbane. The resistance and virulence factors differed significantly between the two sequence types. ST496 colonizing and clinical isolates were similarly multidrug resistant. The virulence genes of ST749 colonizing and clinical isolates were similar as both contained the gene nanB for sialidase. There were no differences in the individual and clinical factors between predominant sequence types. High levels of antimicrobial resistance occurred in the majority of isolates, which is of potential concern to human and veterinary health. The phylogenetic clustering of isolates from this study and others previously identified in countries, particularly New Zealand, with which Australia has high volume of pet movements could suggest the importation of clones, which needs further investigation.

scholarly journals Changes in the Population of Methicillin-Resistant Staphylococcus pseudintermedius and Dissemination of Antimicrobial-Resistant Phenotypes in the Netherlands

2015 ◽  
Vol 54 (2) ◽  
pp. 283-288 ◽  
Author(s):  
Birgitta Duim ◽  
Koen M. Verstappen ◽  
Els M. Broens ◽  
Laura M. Laarhoven ◽  
Engeline van Duijkeren ◽  
...  
Keyword(s):  
The Netherlands ◽  
Otitis Externa ◽  
Temporal Distribution ◽  
Multidrug Resistant ◽  
Wound Infections ◽  
Staphylococcus Pseudintermedius ◽  
Methicillin Resistant ◽  
Content Type ◽  
Clinical Conditions ◽  
Sequence Types

Methicillin-resistantStaphylococcus pseudintermedius(MRSP), which is often multidrug resistant (MDR), has recently emerged as a threat to canine health worldwide. Knowledge of the temporal distribution of specific MRSP lineages, their antimicrobial resistance phenotypes, and their association with clinical conditions may help us to understand the emergence and spread of MRSP in dogs. The aim of this study was to determine the yearly proportions of MRSP lineages and their antimicrobial-resistant phenotypes in the Netherlands and to examine possible associations with clinical conditions. MRSP was first isolated from a canine specimen submitted for diagnostics to the Faculty of Veterinary Medicine of Utrecht University in 2004. The annual cumulative incidence of MRSP amongS. pseudintermediusincreased from 0.9% in 2004 to 7% in 2013. MRSP was significantly associated with pyoderma and, to a lesser extent, with wound infections and otitis externa. Multilocus sequence typing (MLST) of 478 MRSP isolates yielded 39 sequence types (ST) belonging to 4 clonal complexes (CC) and 15 singletons. CC71 was the dominant lineage that emerged since 2004, and CC258, CC45, and several unlinked isolates became more frequent during the following years. All but two strains conferred an MDR phenotype, but strains belonging to CC258 or singletons were less resistant. In conclusion, our study showed that MDR CC71 emerged as the dominant lineage from 2004 and onward and that less-resistant lineages were partly replacing CC71.

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