scholarly journals Changes in the Population of Methicillin-Resistant Staphylococcus pseudintermedius and Dissemination of Antimicrobial-Resistant Phenotypes in the Netherlands

2015 ◽  
Vol 54 (2) ◽  
pp. 283-288 ◽  
Author(s):  
Birgitta Duim ◽  
Koen M. Verstappen ◽  
Els M. Broens ◽  
Laura M. Laarhoven ◽  
Engeline van Duijkeren ◽  
...  
Keyword(s):  
The Netherlands ◽  
Otitis Externa ◽  
Temporal Distribution ◽  
Multidrug Resistant ◽  
Wound Infections ◽  
Staphylococcus Pseudintermedius ◽  
Methicillin Resistant ◽  
Content Type ◽  
Clinical Conditions ◽  
Sequence Types

Methicillin-resistantStaphylococcus pseudintermedius(MRSP), which is often multidrug resistant (MDR), has recently emerged as a threat to canine health worldwide. Knowledge of the temporal distribution of specific MRSP lineages, their antimicrobial resistance phenotypes, and their association with clinical conditions may help us to understand the emergence and spread of MRSP in dogs. The aim of this study was to determine the yearly proportions of MRSP lineages and their antimicrobial-resistant phenotypes in the Netherlands and to examine possible associations with clinical conditions. MRSP was first isolated from a canine specimen submitted for diagnostics to the Faculty of Veterinary Medicine of Utrecht University in 2004. The annual cumulative incidence of MRSP amongS. pseudintermediusincreased from 0.9% in 2004 to 7% in 2013. MRSP was significantly associated with pyoderma and, to a lesser extent, with wound infections and otitis externa. Multilocus sequence typing (MLST) of 478 MRSP isolates yielded 39 sequence types (ST) belonging to 4 clonal complexes (CC) and 15 singletons. CC71 was the dominant lineage that emerged since 2004, and CC258, CC45, and several unlinked isolates became more frequent during the following years. All but two strains conferred an MDR phenotype, but strains belonging to CC258 or singletons were less resistant. In conclusion, our study showed that MDR CC71 emerged as the dominant lineage from 2004 and onward and that less-resistant lineages were partly replacing CC71.

scholarly journals Draft Genome Sequences of 12 Clinical and Environmental Methicillin-Resistant Staphylococcus pseudintermedius Strains Isolated from a Veterinary Teaching Hospital in Washington State

2018 ◽  
Vol 6 (15) ◽  
pp. e00290-18
Author(s):  
Devendra H. Shah ◽  
Lisa P. Jones ◽  
Narayan Paul ◽  
Margaret A. Davis
Keyword(s):  
Teaching Hospital ◽  
Draft Genome ◽  
Multidrug Resistant ◽  
Washington State ◽  
Nosocomial Transmission ◽  
Whole Genome ◽  
Genome Sequences ◽  
Staphylococcus Pseudintermedius ◽  
Methicillin Resistant ◽  
Content Type

ABSTRACT Methicillin-resistant Staphylococcus pseudintermedius (MRSP) is a globally emergent multidrug-resistant pathogen of dogs associated with nosocomial transmission in dogs and with potential zoonotic impacts. Here, we report the draft whole-genome sequences of 12 hospital-associated MRSP strains and their resistance genotypes and phenotypes.

scholarly journals Clonal Diversity and Antimicrobial Resistance of Methicillin-Resistant Staphylococcus pseudintermedius Isolated from Canine Pyoderma

2021 ◽  
Vol 9 (3) ◽  
pp. 482
Author(s):  
Vanessa Silva ◽  
Ana Oliveira ◽  
Vera Manageiro ◽  
Manuela Caniça ◽  
Diogo Contente ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Antimicrobial Agents ◽  
Animal Health ◽  
Clonal Diversity ◽  
Multidrug Resistant ◽  
Diffusion Method ◽  
Staphylococcus Pseudintermedius ◽  
Methicillin Resistant ◽  
Genetic Lineages ◽  
Sequence Types

The emergence of methicillin-resistant Staphylococcus pseudintermedius (MRSP) antimicrobial resistance and epidemic genetic lineages is posing a challenge in veterinary medicine due to the limited therapeutical options. MRSP has been identified as an important canine pyoderma pathogen. Thus, we aimed to characterize the antimicrobial resistance and clonal lineages of MRSP isolated from canine cutaneous pyoderma. Thirty-one MRSP isolates recovered from pyoderma were further characterized. The antimicrobial susceptibility testing of the isolates was performed by the Kirby-Bauer disc diffusion method against 14 antimicrobial agents. The presence of antimicrobial and virulence genes was carried out by PCR. Multilocus sequence typing was performed in all isolates. All strains had a multidrug-resistant profile showing resistance mainly to penicillin, macrolides and lincosamides, aminoglycosides, tetracycline and trimethoprim-sulfamethoxazole, which was encoded by the blaZ, ermB, msr(A/B), aac(6′)-Ie-aph(2′′)-Ia, aph(3′)-IIIa, ant(4′)-Ia, tetM, tetK and dfrG genes. All isolates harbored the lukS-I/lukF-I virulence factors. Isolates were ascribed to nine previously described sequence types (STs): ST123, ST339, ST727, ST71, ST537, ST45, ST1029, ST118 and ST1468; and to five STs first described in this study: ST2024, ST2025, ST2026, ST2027 and ST2028. In this study, most isolates belonged to ST123 (n = 16), which belongs to CC71 and is the most common clone in Europe. All isolates were multidrug-resistant, which may impose a serious threat to animal health.

scholarly journals METHICILLIN-RESISTANT STAPHYLOCOCCUS PSEUDINTERMEDIUS, A NEW THREAT IN HUMAN AND VETERINARY MEDICINE?

2019 ◽  
Vol 12 (2) ◽  
pp. 13-25
Author(s):  
Maja Velhner ◽  
Ljubica Bratić ◽  
Sara Savić ◽  
Dalibor Todorović ◽  
Bojana Prunić ◽  
...  
Keyword(s):  
Clinical Epidemiology ◽  
Multidrug Resistant ◽  
Sequence Type ◽  
Beta Lactam ◽  
Staphylococcus Pseudintermedius ◽  
Methicillin Resistant ◽  
Beta Lactam Antibiotics ◽  
Worldwide Distribution ◽  
Resistance Patterns ◽  
Sequence Types

In this paper we briefl y described the worldwide distribution of methicillin-resistant Staphylococcus pseudintermedius (MRSP) in dogs and cats. Th e most common sequence type of MRSP strains in dogs is ST71 as it was detected in isolates on four continents (Europe, Asia, North and South America). However, several diff erent MRSP sequence types are detected in small animals, and the presence of new genetic variants is continually reported. Sometimes isolates belonging to the same sequence type (ST) are detected in dogs, cats, their owners, and veterinarians. MRSP is oft en multidrug-resistant and its resistance patterns are usually linked to certain sequence types. Th e resistance to non-beta-lactam antibiotics such as erythromycin, clindamycin, tetracycline, gentamicin, enrofl oxacin and sulfamethoxazole/trimethoprim is also recorded. Taking into account that MRSP tends to confer multidrug-resistant phenotype, it is quite challenging for veterinarians to give adequate therapy in clinically ill animals. It would seem as if the signifi cance of MRSP in the clinical epidemiology of humans is not fi rmly established. However, the importance of MRSP in human medicine should not be underestimated given the fact that all methicillinresistant Staphylococcus spp. carry resistance and virulence genes and have the potential to share their genetic elements with other bacteria.

scholarly journals Hybrid Assembly from a Pathogenic Methicillin- and Multidrug-Resistant Staphylococcus pseudintermedius Strain Isolated from a Case of Canine Otitis in Spain

2020 ◽  
Vol 9 (1) ◽  
Author(s):  
Joaquim Viñes ◽  
Anna Cuscó ◽  
Olga Francino
Keyword(s):  
Antimicrobial Resistance ◽  
Genome Assembly ◽  
Hybrid Approach ◽  
Multidrug Resistant ◽  
Nanopore Sequencing ◽  
Hybrid Assembly ◽  
Staphylococcus Pseudintermedius ◽  
Content Type ◽  
Antimicrobial Resistance Profile ◽  
Disk Diffusion Testing

Here we report the genome assembly, using a hybrid approach with Illumina and Nanopore sequencing, of a pathogenic Staphylococcus pseudintermedius strain isolated from a case of canine otitis. Genome assembly confirmed the antimicrobial resistance profile (disk diffusion testing) with specific genes and mutations.

scholarly journals The Resistome of Pseudomonas aeruginosa in Relationship to Phenotypic Susceptibility

2014 ◽  
Vol 59 (1) ◽  
pp. 427-436 ◽  
Author(s):  
Veronica N. Kos ◽  
Maxime Déraspe ◽  
Robert E. McLaughlin ◽  
James D. Whiteaker ◽  
Paul H. Roy ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Multidrug Resistant ◽  
Clinical Isolates ◽  
Genetic Determinants ◽  
Content Type ◽  
Susceptibility Data ◽  
Next Generation Sequencing Ngs ◽  
Sequence Types ◽  
Generation Sequencing ◽  
Phenotypic Susceptibility

ABSTRACTMany clinical isolates ofPseudomonas aeruginosacause infections that are difficult to eradicate due to their resistance to a wide variety of antibiotics. Key genetic determinants of resistance were identified through genome sequences of 390 clinical isolates ofP. aeruginosa, obtained from diverse geographic locations collected between 2003 and 2012 and were related to microbiological susceptibility data for meropenem, levofloxacin, and amikacin. β-Lactamases and integron cassette arrangements were enriched in the established multidrug-resistant lineages of sequence types ST111 (predominantly O12) and ST235 (O11). This study demonstrates the utility of next-generation sequencing (NGS) in defining relevant resistance elements and highlights the diversity of resistance determinants withinP. aeruginosa. This information is valuable in furthering the design of diagnostics and therapeutics for the treatment ofP. aeruginosainfections.

scholarly journals Targeting Methicillin-Resistant Staphylococcus aureus with Short Salt-Resistant Synthetic Peptides

2014 ◽  
Vol 58 (7) ◽  
pp. 4113-4122 ◽  
Author(s):  
Mohamed F. Mohamed ◽  
Maha I. Hamed ◽  
Alyssa Panitch ◽  
Mohamed N. Seleem
Keyword(s):  
Staphylococcus Aureus ◽  
Synthetic Peptides ◽  
Bactericidal Effect ◽  
Multidrug Resistant ◽  
Antimicrobial Activities ◽  
Net Charge ◽  
Methicillin Resistant ◽  
Content Type ◽  
C Terminus ◽  
Conventional Antibiotics

ABSTRACTThe seriousness of microbial resistance combined with the lack of new antimicrobials has increased interest in the development of antimicrobial peptides (AMPs) as novel therapeutics. In this study, we evaluated the antimicrobial activities of two short synthetic peptides, namely, RRIKA and RR. These peptides exhibited potent antimicrobial activity againstStaphylococcus aureus, and their antimicrobial effects were significantly enhanced by addition of three amino acids in the C terminus, which consequently increased the amphipathicity, hydrophobicity, and net charge. Moreover, RRIKA and RR demonstrated a significant and rapid bactericidal effect against clinical and drug-resistantStaphylococcusisolates, including methicillin-resistantStaphylococcus aureus(MRSA), vancomycin-intermediateS. aureus(VISA), vancomycin-resistantS. aureus(VRSA), linezolid-resistantS. aureus, and methicillin-resistantStaphylococcus epidermidis. In contrast to many natural AMPs, RRIKA and RR retained their activity in the presence of physiological concentrations of NaCl and MgCl2. Both RRIKA and RR enhanced the killing of lysostaphin more than 1,000-fold and eradicated MRSA and VRSA isolates within 20 min. Furthermore, the peptides presented were superior in reducing adherent biofilms ofS. aureusandS. epidermidiscompared to results with conventional antibiotics. Our findings indicate that the staphylocidal effects of our peptides were through permeabilization of the bacterial membrane, leading to leakage of cytoplasmic contents and cell death. Furthermore, peptides were not toxic to HeLa cells at 4- to 8-fold their antimicrobial concentrations. The potent and salt-insensitive antimicrobial activities of these peptides present an attractive therapeutic candidate for treatment of multidrug-resistantS. aureusinfections.

scholarly journals Comparative RNA-seq-Based Transcriptome Analysis of the Virulence Characteristics of Methicillin-Resistant and -Susceptible Staphylococcus pseudintermedius Strains Isolated from Small Animals

2015 ◽  
Vol 60 (2) ◽  
pp. 962-967 ◽  
Author(s):  
Natacha Couto ◽  
Adriana Belas ◽  
Manuela Oliveira ◽  
Paulo Almeida ◽  
Carla Clemente ◽  
...  
Keyword(s):  
Virulence Genes ◽  
Expression Profiles ◽  
Brain Heart Infusion ◽  
Surface Proteins ◽  
Rna Seq ◽  
Staphylococcus Pseudintermedius ◽  
Methicillin Resistant ◽  
Content Type ◽  
Genes Encoding

ABSTRACTStaphylococcus pseudintermediusis often associated with pyoderma, which can turn into a life-threatening disease. The dissemination of highly resistant isolates has occurred in the last 10 years and has challenged antimicrobial treatment of these infections considerably. We have compared the carriage of virulence genes and biofilm formation between methicillin-resistant and methicillin-susceptibleS. pseudintermedius(MRSP and MSSP, respectively) isolates and theirin vitrogene expression profiles by transcriptome sequencing (RNA-seq). Isolates were relatively unevenly distributed among the fouragrgroups, andagrtype III predominated in MRSP. Five virulence genes were detected in all isolates. Only thespsOgene was significantly associated with MSSP isolates (P= 0.04). All isolates produced biofilm in brain heart infusion broth (BHIB)–4% NaCl. MSSP isolates produced more biofilm on BHIB and BHIB–1% glucose media than MRSP isolates (P= 0.03 andP= 0.02, respectively). Virulence genes encoding surface proteins and toxins (spsA,spsB,spsD,spsK,spsL,spsN,nucC,coa, andluk-I) and also prophage genes (encoding phage capsid protein, phage infection protein, two phage portal proteins, and a phage-like protein) were highly expressed in the MRSP isolate (compared with the MSSP isolate), suggesting they may play a role in the rapid and widespread dissemination of MRSP. This study indicates that MRSP may upregulate surface proteins, which may increase the adherence of MRSP isolates (especially sequence type 71 [ST71]) to corneocytes. MSSP isolates may have an increased ability to form biofilm under acidic circumstances, through upregulation of the entirearcoperon. Complete understanding ofS. pseudintermediuspathogenesis and host-pathogen signal interaction during infections is critical for the treatment and prevention ofS. pseudintermediusinfections.

scholarly journals Epidemiology of Staphylococcus pseudintermedius in cats in Poland

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
K. Bierowiec ◽  
M. Miszczak ◽  
A. Korzeniowska-Kowal ◽  
A. Wzorek ◽  
D. Płókarz ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Multidrug Resistance ◽  
Biofilm Formation ◽  
Clinical Signs ◽  
Multidrug Resistant ◽  
Health Condition ◽  
Staphylococcus Pseudintermedius ◽  
First Time ◽  
Sequence Types ◽  
Antibiotic Resistance Properties

AbstractStaphylococcus pseudintermedius is a well-known coagulase-positive staphylococcus that is mainly associated with the asymptomatic colonization of the skin of pets and mucous membranes. Little is still known about the occurrence of S. pseudintermedius in cats. The current study aimed to characterize the isolates of S. pseudintermedius from sick and healthy cats. This was achieved by examining their antibiotic resistance properties, biofilm formation, and genotype differences. Six hundred and seventy-six cats were swabbed (595 healthy and 81 sick cats). Thirty-five distinct S. pseudintermedius isolates from 27 cats were isolated. The prevalence of S. pseudintermedius in healthy and sick cats was 2.49% and 7.61%, respectively. In comparison, MRSP (methicillin-resistant Staphylococcus pseudintermedius) prevalence was 0.12% and 2.98%, respectively. Cats were more frequently colonized with S. pseudintermedius when kept with dogs, regardless of their health condition, with this result being statistically significant. Multidrug resistance was detected in 50%, and 38.46% of S. pseudintermedius isolates from healthy and sick cats, respectively. In contrast, genetic multidrug resistance was detected in 59% and 46.15% cases, respectively. Seven from eight isolated MRSPs were multidrug-resistant. Multi-locus sequence typing (MLST) assigned isolates to 19 types, of which 16 types submitted for the first time to the PubMLST database. The most frequently detected STs (sequence types) were 551 and 71. ST71 and ST551 were mainly isolated from cats with clinical signs of infection. All were MRSPs, regardless of cats’ health. These isolates were characterized with the most frequent antibiotic resistance at the phenotypic and genotypic level.

scholarly journals In VitroActivity of Dalbavancin against Drug-Resistant Staphylococcus aureus Isolates from a Global Surveillance Program

2015 ◽  
Vol 59 (8) ◽  
pp. 5007-5009 ◽  
Author(s):  
Sandra P. McCurdy ◽  
Ronald N. Jones ◽  
Rodrigo E. Mendes ◽  
Sailaja Puttagunta ◽  
Michael W. Dunne
Keyword(s):  
Staphylococcus Aureus ◽  
Drug Administration ◽  
Food And Drug Administration ◽  
Multidrug Resistant ◽  
Surveillance Program ◽  
Drug Resistant ◽  
Methicillin Resistant ◽  
Content Type

ABSTRACTIn over a decade (2002 to 2012) ofStaphylococcus aureussurveillance testing on 62,195 isolates, dalbavancin was demonstrated to be active against isolates that were either susceptible or nonsusceptible to daptomycin, linezolid, or tigecycline. Nearly all (99.8%) multidrug-resistant methicillin-resistantS. aureusisolates were inhibited by dalbavancin at ≤0.12 μg/ml (MIC50/90, 0.06/0.06 μg/ml), the current U.S. Food and Drug Administration (U.S. FDA) breakpoint. Overall, only 0.35% of the monitoredS. aureusisolates had a dalbavancin MIC of either 0.25 or 0.5 μg/ml (i.e., were nonsusceptible).

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