Characterizing the structural and surface properties of proteins isolated before and after enzymatic demulsification of the aqueous extract emulsion of peanut seeds

2015 ◽  
Vol 47 ◽  
pp. 51-60 ◽  
Author(s):  
Shao Bing Zhang ◽  
Qi Yu Lu
Author(s):  
Sri Indrawati ◽  
Yuliet Yuliet ◽  
Ihwan Ihwan

Pisang Ambon (Musa paradisiaca L.) is one type of bananas usually consumed by Indonesian people. Besides its flesh which has high nutrition, its peels also has antioxidant activity. Antioxidants has the ability to reduce oxidative damage in people’s body with diabetes mellitus. Therefore, this study aimed to determine the antioxidant activity of the aqueous  extract of Pisang Ambon peels and to determine it’s effective dose as an antidiabetic agent in hyperglycemic mice. This study used male mice which all have been intravenously induced with alloxan at a dose of 50 mg/kgBW. They were then divided into five groups. The first two groups got Na CMC 0.5% (negative control) and glibenclamide 0.65 mg/kgBW (positive control), while the other three got  the aqueous  extract of Pisang Ambon peels successively at doses of 400, 800, and 1200 mg/kgBW. The data were statistically analyzed using ANOVA (Analysis of Variance) at 95% confidence interval with parameter of blood glucose levels difference between before and after treatment. The results showed that the aqueous extract of Pisang Ambon peels had antidiabetic activity at an effective dose of 400 mg/kgBW in hyperglycemic mice which was comparable to glibenclamide


2012 ◽  
Vol 727-728 ◽  
pp. 1616-1621 ◽  
Author(s):  
Boeira Braga Matheus ◽  
Cristina dos Santos Rocha Sandra

In this research, glass beads with size range between 1.68 and 2mm were coated with 5 polymeric suspensions. The suspensions formulations differ in relation to their employment and physical properties (solids concentration, surface tension and rheology), generating different characteristics of wettability and adhesion with the nucleus. The aim of this study was to evaluate particle coating in a spouted bed through analysis of particle growth in terms of solid surface energy, wettability, and adhesion before and after the formation of the first layer of polymeric film on the particle. The solid-suspension and film-suspension systems were characterized by contact angle and surface energy. The operating conditions were fixed for all suspensions: 1.5 kg of beads, air velocity of 0.369 m/s, air temperature of 60 °C, suspension flow rate of 4 ml and atomizing pressure of 10 psig. Analyzing particle growth kinetics, different behaviors were observed and related not only to glass-suspension wettability, but also to polymeric film-suspension surface properties.


2018 ◽  
Vol 26 (6(132)) ◽  
pp. 114-119 ◽  
Author(s):  
Magdalena Ziąbka ◽  
Michał Dziadek

The aim of this study was to investigate the surface properties of polymeric composites and the osteoblastic cell behaviour set in direct contact with the biomaterials tested. The surface properties were evaluated before and after 6-month incubation in an in vitro environment. The composite materials were prepared by means of extrusion and injection moulding. Three commercially available thermoplastic polymers (ABS (poly)acrylonitrile butadiene styrene) were used as composite matrices. Antibacterial silver nanoparticles (AgNPs) were added as a modifying phase. Surface properties of the materials tested, such as: wettability, roughness and microstructure, were determined. Furthermore the morphology of Saos-2 human osteoblastic cells in direct contact with the composite materials was assessed after the 7-day culture. The addition of silver nanoparticles caused minor changes in the wettability and roughness values. As light modification, the silver nanoparticles influenced the microstructure. The osteoblasts displayed the proper morphology and they evenly settled on the surface of the pure polymer and composite materials, which indicated the material cytocompatibility.


2018 ◽  
Vol 2018 ◽  
pp. 1-14 ◽  
Author(s):  
Stéphane Zingue ◽  
Harquin Simplice Foyet ◽  
Sefirin Djiogue ◽  
Yannick Ezo’o Ezo’o ◽  
Hervé Hervé Ngatanko Abaïssou ◽  
...  

The present work was undertaken to evaluate the ability of F. umbellata aqueous extract and its major component 7-methoxycoumarin (MC) to improve scopolamine-induced spatial memory impairment in ovariectomized Wistar rats. For this to be done, 10 sham-operated and 30 postmenopausal-like rats were randomly distributed in eight groups (n=5) and treated with distilled water (2 mL/250 g), estradiol valerate (1 mg/kg BW), piracetam (1.5 mg/kg BW), F. umbellata aqueous extract (50 and 200 mg/kg BW), or MC (1 mg/kg BW) for 21 consecutive days. Before and after the memory impairment with scopolamine (2 mg/kg BW), animals underwent behavioral evaluations on Y- and radial mazes. As results, age and ovariectomy did not induce significant changes in the reference memory errors. While age decreased working memory errors, ovariectomy increased it. The MC as well as F. umbellata extract significantly increased (p<0.01) the percentage of spontaneous alternation and decreased (p<0.001) working and spatial reference memory errors and anxiety parameters (rearing and grooming) in ovariectomized rats. MC significantly reduced (p<0.05) the MDA level, but resulted in an increase in GSH level in brain homogenates. These results suggest that MC is endowed with neuroprotective effects and could account for the neuroprotective effects of F. umbellata in rats.


2016 ◽  
Vol 2016 ◽  
pp. 1-6 ◽  
Author(s):  
Ji Hee Kim ◽  
Su Kyung Lee ◽  
Min Cheol Joo

Objective. To investigate the effects and safety of the aqueous extract of the dried, immature fruit ofPoncirus trifoliata(L.) Raf., known asPoncirus fructus(PF), in spinal cord injury (SCI) patients with neurogenic bowel.Methods. Thirty-one SCI patients with neurogenic bowel were recruited. Patients were evaluated based on clinical information, constipation score, Bristol Stool Form Scale, stool retention score using plain abdominal radiograph, and colon transit time. PF was administered in dosages of 800 mg each prior to breakfast and lunch for 14 days.Results. The morphological feature of the stool before and after administration indicated a statistically significant difference from 3.52 ± 1.33 to 4.32 ± 1.44 points (p<0.05). Stool retention score before and after administration of PF was represented with low significance (7.25 ± 1.60 to 6.46 ± 1.53 points) in the whole colon (p<0.05), and the colon transit time was significantly shortened (57.41 ± 20.7 to 41.2 ± 25.5 hours) in terms of the whole transit time (p<0.05). Side effects were observed in 7 people (28.0%) consisting of 2 people with soft stools and 5 people with diarrhea.Conclusion. For SCI patients, PF administration significantly improved defecation patterns, defecation retention, and colon transit time. PF could be an effective aid to improve colonic motility and constipation.


2016 ◽  
Vol 45 (2) ◽  
pp. 103-109
Author(s):  
Brenna Louise Cavalcanti GONDIM ◽  
Isabella Cavalcante MEDEIROS ◽  
Bruna Palmeira COSTA ◽  
Hugo Lemes CARLO ◽  
Rogério Lacerda dos SANTOS ◽  
...  

Abstract Introduction Few studies investigated the surface properties of luting cements after erosive challenge. Objective To evaluate the surface roughness (Ra), Vickers hardness (VHN) and morphology of 4 luting cements after erosive challenge. Material and method Twenty specimens of each cement were prepared (4×2mm) and divided into experimental (erosive challenge) and control (artificial saliva) groups (n=10): Rely X U200 (U200); Rely X ARC (ARC); Ketac Cem Easy Mix (Ketac) and Zinc phosphate (ZnP). The erosive challenge was performed by four daily erosive cycles (90s) in a cola drink and 2 h in artificial saliva over 7 days. Ra and VHN readings were performed before and after erosion. The percentage of hardness loss (%VHN) was obtained after erosion. The surface morphology was analyzed by scanning electron microscopy (SEM). ANOVA, Tukey and Student-T tests were used (α=0.05). Result After erosion, all luting cements had increase in Ra values and U200 and ZnP groups had the highest %VHN. After saliva immersion, only U200 and ZnP groups had significant increases in Ra values and there were no significant differences among the groups in %VHN. SEM analysis showed that Ketac and ZnP groups had rough and porous surfaces, and U200 group had higher resin matrix degradation than ARC group. Conclusion Erosive challenge with a cola drink affected the surface properties of all luting cements.


Author(s):  
ADETUNJI OPEYEMI ADEBOLA ◽  
OYEWO OLUWATOYIN ◽  
ADETUNJI IYABODE TOYIN ◽  
NWOBI NNENA LINDA

Aim and Objectives: Demonstrating the exact quantity of Moringa oleifera (MO) that will cure any hepatocyte diseases and the one that can harm the hepatocytes of the Adult Wistar rats. Methods: Twenty (20) adult Wistar rats (both sex) were used for the study (caged based on same sex to avoid mating and pregnancy) and were randomly assigned into four groups (n=5); A-Control, B-250 mg/body weight (BW) of MO, C-500 mg/BW of MO, and D-750 mg/BW of MO orally. Result: The crude aqueous extract of MO Lam, shows insignificant increased in BW at the 1st week of administration which latter dropped little by little doing the weeks of the administration in groups (B, C, and D) rats, by comparing (p<0.05) to the control group after MO administration, the organ (Kidneys) shows a significant difference between the kidneys (left and right kidneys) in relation to the control group rats. MO increases the weight of the animals morphologically by comparing the weight of the animals before and after administration. Histological sections shows a normal Glomerulli, Peri-Glomerular Space, Convoluted Tubules, and Interstitium, after administration of the Crude Aqueous Extract of MO lame in terms with the control group rats. Conclusion: MO is a good herb that has no damage effect on the body and hepatocytes but of more beneficial.


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