HISTOLOGICAL VARIANCES OF MORINGA OLIFERA ON THE KIDNEY OF ADULT WISTAR RATS

Aim and Objectives: Demonstrating the exact quantity of Moringa oleifera (MO) that will cure any hepatocyte diseases and the one that can harm the hepatocytes of the Adult Wistar rats. Methods: Twenty (20) adult Wistar rats (both sex) were used for the study (caged based on same sex to avoid mating and pregnancy) and were randomly assigned into four groups (n=5); A-Control, B-250 mg/body weight (BW) of MO, C-500 mg/BW of MO, and D-750 mg/BW of MO orally. Result: The crude aqueous extract of MO Lam, shows insignificant increased in BW at the 1st week of administration which latter dropped little by little doing the weeks of the administration in groups (B, C, and D) rats, by comparing (p<0.05) to the control group after MO administration, the organ (Kidneys) shows a significant difference between the kidneys (left and right kidneys) in relation to the control group rats. MO increases the weight of the animals morphologically by comparing the weight of the animals before and after administration. Histological sections shows a normal Glomerulli, Peri-Glomerular Space, Convoluted Tubules, and Interstitium, after administration of the Crude Aqueous Extract of MO lame in terms with the control group rats. Conclusion: MO is a good herb that has no damage effect on the body and hepatocytes but of more beneficial.

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The effect of ethanol extract of pegagan (Centella asiatica) on the performance of Wistar rats after restraint stress

Biofarmasi Journal of Natural Product Biochemistry ◽

10.13057/biofar/f080102 ◽

2010 ◽

Vol 8 (1) ◽

pp. 11-16

Author(s):

BAARID LUQMAN HAMIDI ◽

SAMIGUN SAMIGUN ◽

ANIK LESTARI

Keyword(s):

Restraint Stress ◽

Wistar Rats ◽

Radial Maze ◽

Centella Asiatica ◽

Ethanol Extract ◽

The Body ◽

Control Group ◽

Error Type ◽

Stress Group ◽

Significant Difference

Hamidi BL, Samigun, Lestari A. 2010. The effect of extract ethanol of pegagan (Centella asiatica) on the performance of Wistar rats after restraint stress. Biofarmasi 8: 11-16. The aim of this research was to investigate the effects of extract ethanol of pegagan (Centella asiatica) after treated with restraint stress by measuring the eight arms radial maze performance of rats. Pre-test and post-test controlled groups design was applied in this research. Male Wistar rats (Rattus novergicus) with the mean age of 8 weeks and the body weight of 150-200 grams which used for sample were divided randomly into 4 groups, each group consisted of 6 rats, i.e. (i) control group (without any treatment), (ii) stress group (it was given by restraint stress for 2 hours/day for each rat), (iii) pegagan group (it was given by 0.3 mg/g BW/day/rat extract ethanol of pegagan), and (iv) pegagan and stress group (it was given by 0.3 mg/g BW/day/rat extract ethanol of pegagan and restraint stress for 2 hours/day for each rat). The treatments were given for 21 days. Within 12 days for each pre-treatment and post-treatment, a test on the eight arm radial maze was conducted on individual rat to observe its performance. The assessment of rat performance in the eight arms radial maze test was conducted based on error type B. Kruskal-Wallis and Mann-Whitney tests with SPSS for Windows 16 version were applied to analyze statistically the difference between four groups. Kruskal-Wallis test was used to show the significant performance level difference between four groups of rats with p=0.001, while Mann-Whitney test was used to determine the significant difference between stress group and pegagan group (p=0.001), also stress group and pegagan and stress group (p=0.001). The result of research showed that there was no significant difference between control group and stress group (p=0.051), control group and pegagan group (p=0.143), control group and pegagan and stress group (p=0.143), also pegagan group and pegagan and stress group (p=0.952). It was concluded that extract ethanol of pegagan improved the performances of rats on the eight arms radial maze after treated with restraint stress.

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Pengaruh Infusa Biji Buah Pinang (Areca catechu) Terhadap Tingkat Kematian dan Morfometri Cacing Dewasa Ascaridia galli Secara In Vitro

Jurnal Sain Veteriner ◽

10.22146/jsv.43751 ◽

2019 ◽

Vol 37 (2) ◽

pp. 166

Author(s):

Wida Wahidah Mubarokah ◽

Kurniasih Kurniasih ◽

Wisnu Nurcahyo ◽

Joko Prastowo

Keyword(s):

Mortality Rate ◽

Aqueous Extract ◽

Saline Water ◽

The Body ◽

Ascaridia Galli ◽

Body Width ◽

Significant Difference ◽

Areca Catechu ◽

Crude Aqueous Extract

The study evaluates in vitro effects of Areca catechu crude aqueous extract as anthelmintics on Ascaridia galli adult mortality rate and morphometry. Naturally infected chickens were collected from slaughtered chickens at local slaughterhouses in Yogyakarta. Chicken’s intestines were carefully examined and transported to the Parasitological Laboratory of Gadjah Mada University, Yogyakarta. Ascaridia galli were collected from intestines (including duodenum, jejunum, and ileum) and put into a petri dish containing 0.62% saline water. There were 60 A. galli in each concentration of Areca catechu crude aqueous extract. Investigation on mortality rate of adult worms at various concentration and observation as well as differences in A. galli morphometry and mortality rate were analyzed using analysis Anova. The figures of the parasites or the parts of the parasites were captured using camera Lucida and then they were measured using both micrometer and curvimeter. The morphology of the parasites was identified to find out their morphometric characteristics.The results of morphometric observation of the A. galli showed that there was a significant difference in the body width and the vulva length of the females and in the body width, the esophageal length and the esophageal width of the males.This research disclosed that Areca catechu crude aqueous extract significantly affected the mortality rate of adult A.galli at various concentration. This research observed that the best concentration to kill the adult worms was 25% Areca catechu crude aqueous extract.

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The Effect of Moringa Leaf Capsule on the Hemoglobin Levels in Young Women at Smp Sabbihisma Padang

KnE Life Sciences ◽

10.18502/kls.v4i15.5753 ◽

2019 ◽

Author(s):

Wiwi Sartika ◽

Herwati . ◽

Yossi Suryarinilsih

Keyword(s):

Young Women ◽

Junior High School ◽

The Body ◽

Control Group ◽

Case Group ◽

Control Groups ◽

Control Group Design ◽

Learning Abilities ◽

Significant Difference ◽

Before And After

In adolescents, the iron not only functions as energy metabolism in the body but also helps to improve learning achievement. Iron deficiency can lead to fatigue, and the concentration, memory and learning abilities can be disrupted. It can also lead to anemia, especially in young women. The purpose of this study was to determine the effect of leaf moringa capsules on the hemoglobin levels in adolescent girls in Sabbihisma Junior High School. This research used a quasi method of pretest and posttest experiment with control group design. The sample of the study were 32 people, 16 cases (moringa leaf capsule) and 16 control groups. Data collection was done through observation and laboratory examination of hemoglobin levels before and after treatment. The data were tested with paired sample t-test. The results showed that there was a significant difference in female hemoglobin levels in the case group with p = 0,000, while the control group was not significant with p = 0,091.

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Evaluation of 90-day subchronic oral toxicity of aqueous extract of Gmelina arborea Roxb (Verbenaceae) leaves in Wistar rats

Tropical Journal of Pharmaceutical Research ◽

10.4314/tjpr.v19i3.23 ◽

2020 ◽

Vol 19 (3) ◽

pp. 617-622

Author(s):

Razack Osseni ◽

Azonbakin Simon ◽

Diallo Aboudoulatif ◽

Habib Ganfon ◽

Adjagba Marius ◽

...

Keyword(s):

Food Intake ◽

Aqueous Extract ◽

Wistar Rats ◽

The Body ◽

Biological Parameters ◽

Gmelina Arborea ◽

Oral Toxicity ◽

Subchronic Toxicity ◽

Significant Difference ◽

Body Weights

Purpose: To evaluate the 90 day sub-chronic toxicity of aqueous extract of Gmelina arborea leaves in Wistar rats. Methods: Rats were submitted to repeated daily oral administration of extract (250, 62.5 and 15.62 mg/kg) of Gmelina arborea leaves. The control groups were given distilled water and the rats were monitored for any toxicity symptoms as well as body and organs weights, water and food intake changes. The biochemical, haematological and histolopathological parameters were analysed. Results: The 90 days administration of the aqueous extract did not produce any toxicity signs or mortality. In addition, no significant alteration in water or food intake by the rats was observed. Although there were no changes in the body weights, significant decrease in the weight of the kidneys of the rats was observed at 250 mg/kg. Biological parameters as well as the histopathology of liver and kidneys were not significantly affected. Significant decreases were noted in glucose level at the three dose levels. In addition, significant difference in the levels of transaminases, glucose and platelets were observed. Conclusion: The 90-days subchronic toxicity test on Gmelina arborea did not produce any toxic effects. This confirms the safety of the plant leaves by traditional medicine practitioners. Keywords: Gmelina arborea, Subchronic toxicity, Wistars rats, Biological parameters

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Effects of Moringa oleifera on the developing cerebrum of young wistar rats

Anatomy Journal of Africa ◽

10.4314/aja.v8i1.182596 ◽

1970 ◽

Vol 8 (1) ◽

Author(s):

A Z Abijo ◽

O O Adeeyo ◽

O A Komolafe ◽

O S Saka ◽

V K Abodunrin

Keyword(s):

Frontal Cortex ◽

Wistar Rats ◽

Moringa Oleifera ◽

The Body ◽

Brain Weight ◽

Control Group ◽

Significant Difference ◽

Group A ◽

High Doses ◽

Group B

The study evaluated the effects of moringa oleifeira on the histoarchitecture of the cerebral cortex, the body weight and brain weight of young wistar rats. Fifteen (15) young wistar rats of both sexes weighing 20-30g were used for this study. They were randomly assigned into three groups (A, B and C) of five rats each. Group A served as control and received distilled water, group B and C received 100 mg/kg and 200mg/kg of moringa oleifera respectively. Treatment lasted for a period of 6 weeks (orally). Rats were weighed and sacrificed under ketamine (30 mg/kg) anaesthesia. The cerebrum was harvested and fixed immediately in 10% formolcalcium, for further histological processing. One-way ANOVA was used to analyze data, followed by Newan-Keuls (SNK) for multiple comparisons. The results showed that there was significant increase in the feed intake of animals in groups B and C starting from the 4th week of administration. There was no significant difference in the relative brain weight and the mean weight of the rats in group B and C when compared with group A. Histological findings revealed that there was slight distortion in group B and more distortion in group C when compared with the normal histoarchitecture in control group A. The results obtained from this study showed that high doses of Moringa oleifera caused damage of some parts of histoarchitecture of the frontal cortex of developing wistarKey words: MORINGA OLEIFERA; Cerebrum; Frontal Cortex

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Perbedaan Tekanan Darah Sebelum dan Sesudah Exercise Intradialysis Pada Pasien Hemodialisis di Unit Hemodialisis Rumah Sakit Panti Rahayu Gunung Kidul

I Care Jurnal Keperawatan STIKes Panti Rapih ◽

10.46668/jurkes.v2i2.196 ◽

2021 ◽

Vol 2 (2) ◽

pp. 156-169

Author(s):

Christina Murni Yuliastuti ◽

Th.Tatik Pujiastuti ◽

Sr. Lucilla Suparmi, CB

Keyword(s):

Blood Pressure ◽

Intervention Group ◽

The Body ◽

Control Group ◽

Cardiovascular Exercise ◽

Significant Difference ◽

Before And After ◽

Quasi Experimental ◽

The Difference ◽

Intradialytic Exercise

ABSTRACT Background:Hemodialysis defines as a process of cleaning the blood from waste substances through a filtering process outside the body. Patients with chronic renal failure undergoing hemodialysis often experience complications including hypotension. Several references state an alternative intervention to prevent complications of hypotension in hemodialysis patients with an Intradialytic exercise. Intradialytic exercise is a planned and gradual form of exercise that includes various stages of flexibility exercise, strengthening exercise and cardiovascular exercise performed during hemodialysis. Intradialytic exercise is aimed to improve the work of the heart, respiration and improve hemodialysis regulation for the better. Objective:This study was aimed to determine the difference in blood pressure before and after intradialytic exercise in patients undergoing hemodialysis. Methods:This research design used a quasi-experimental design with pre-test and post-test with control design. The samples were 38 respondents who taken by total sampling at the Hemodialysis Unit of Panti Rahayu Hospital. The samples were divided into the intervention group who undertook intradialytic exercise for 4 weeks, each respondent experiences twice a week, while the control group who did routine hemodialysis and independent exercise. Results:The results showed that the distribution of the characteristics of the respondents was 51-54 years old (18.41%) the sex was mostly male (63.2%) Most of them (55.3%) underwent hemodialysis for less than 2 years. Statistically, it was known that there was no significant difference in blood pressure before and after intradialytic exercise inside patient’s body of the control and intervention groups. There was no significant difference in blood pressure between the intervention group compared to the control group, but there was a dynamic difference in blood pressure in patients who did intradialytic exercise. Conclusion:There was dynamics of differences in blood pressure in patients undergoing intradialytic exercise, it is recommended that hemodialysis nurses at Panti Rahayu Hospital take care patients during hemodialysis so that these interventions are routinely carried out.

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The effect of using Electrical Flipping Model Learning to improve the performance of student in Electric and Electronic of Automobiles

ALUSTATH JOURNAL FOR HUMAN AND SOCIAL SCIENCES ◽

10.36473/ujhss.v226i3.93 ◽

2018 ◽

Vol 226 (3) ◽

pp. 229-248

Author(s):

Lecturer: Majida Waheeb Irzooqi

Keyword(s):

Control Group ◽

Model Learning ◽

Traditional Course ◽

Significant Difference ◽

Before And After ◽

Training Of Trainers ◽

And Performance ◽

The One ◽

And Control ◽

Experimental Group

The aim of the study was to uncover the effectiveness of the inverted learning model in the achievement and performance of e-learning skills in the students of the department of machinery and equipment . The semi experimental approach design was used with before and after test measurement. The study sample consisted of students of the first stage of the equipment and equipment department of the Institute of training of trainers (70) students divided into two groups: experimental group (35) students were taught the subject of electricity and electronic cars. And a control group (35) students were taught with a traditional course. The tools of the study were applied before and after the two groups. The data were analyzed using the one-way contrast test and Gohen Standard equation to effective size . The results of the study were statistically significant difference at (α = 0.05k) between the average of the experimental and control groups in the post application of both the achievement test and the skill performance observation card for the benefit of the experimental group.

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Bagian Biomedis Fakultas Kedokteran Gigi, Universitas Jember Jember, Indonesia

Journal of Dentomaxillofacial Science ◽

10.15562/jdmfs.v10i1.248 ◽

2011 ◽

Vol 10 (1) ◽

pp. 26

Author(s):

I Dewa Ayu Ratna Dewanti

Keyword(s):

Traditional Medicine ◽

Aqueous Extract ◽

Wistar Rats ◽

The Other ◽

Control Group ◽

Aqueous Extracts ◽

Treatment Groups ◽

Neem Leaves ◽

Significant Difference ◽

Phagocytosis Activity

Neem is one of the traditional medicine known by society as immunomodulator. On the other hand, 80% of oralinfection diseases is caused by C. albicans. This research is aimed to explain the phagocytosis activities on wistarrats which were inoculated with C. albicans and fed with neem leaves. There are 5 groups, namely control group(KO)with no treatment, the other treatment groups were classified into 4 groups. First group (KP1)was inoculatedwith C. albicans only. KP2 was fed with 50 mg/day/kg BW aqueous extracts from neem leaves, then inoculated withC. albicans start from day 8-21. KP3 was fed with 100 mg/day/kg BW aqueous extract from neem leaves, theninoculated with C. albicans start from day 8-21. KP4 was fed with 200 mg/day/kg BW aqueous extract from neemleaves, then inoculated with C. albicans start from day 8-21. On day 22, the tongue was swabbed for each group,then cut for immunohistochemistry preparation. The study that there was significant difference showed that therewere different results computed using anova, HSD test, and linier regression. The conclusion was neem leavesincreased the phagocytosis activity of wistar rats, inoculated with C.albicans.

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Evaluation of the Effects of Aqueous Fruit Extract of Tamarindus indica on Body Weight, Lipid Profile, Some Electrolytes and Urea of Wistar Rats

Journal of Pharmaceutical Research International ◽

10.9734/jpri/2021/v33i59a34290 ◽

2021 ◽

pp. 444-451

Author(s):

I. P. Ekpe ◽

Dennis Amaechi ◽

B. N. Yisa ◽

O. G. Osarhiemen

Keyword(s):

Body Weight ◽

Aqueous Extract ◽

Wistar Rats ◽

Low Density Lipoprotein ◽

Density Lipoprotein ◽

Control Group ◽

Low Density ◽

Healthy Weight ◽

Group I ◽

Significant Difference

Objectives: The objectives of this research is to evaluate the effects of aqueous extract of T. indica on weight, lipid parameters, some electrolytes and urea of Wistar rats Design & Methods: Twenty-five Male Adult rats were grouped into four consisting of six rats each. Group I served as control group, group II was administered 100mg/kg of the Aqueous extract, group III was administered 150mg/kg of the Aqueous extract, and Group IV was administered 200mg/kg of the Aqueous extract. The administration took place for seven days, and on the eight day, The Animals were sacrificed. The blood samples was collected via cardiac puncture and used for analysis using a visible spectrophotometer. Results: From the result, there was no significant difference (p<0.05) in body weight of Wistar rats. In the Test Groups; Serum cholesterol, low density lipoprotein (LDL), High density lipoprotein (HDL), Very Low-density Lipoprotein and Triglycerides revealed a significant increase (p<0.05) compared to Group 1 which is the control Group but had a significant decrease (p<0.05) compared within test groups. For electrolytes concentrations, potassium, Sodium, Chloride and Urea concentrations was significant at p<0.05. Conclusion: the results above ,T. indica extract may help to maintain healthy weight, may have hypolipidemic properties, may help in body homeostatic and fluid balance and may also prevent renal damages.

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THE EFFECT OF CUPPING THERAPY ON THE SCALE OF PAIN IN ADVANCED AGE WHO EXPERIENCED JOINT PAIN IN JEMBER

International Journal of Islamic Medicine ◽

10.37275/ijim.v1i1.3 ◽

2020 ◽

Vol 1 (1) ◽

pp. 21-29

Author(s):

Istiadhatul Magfiroh ◽

Wahyudi Widada ◽

Sofia Rhosma Dewi

Keyword(s):

Pain Intensity ◽

Joint Pain ◽

Cupping Therapy ◽

The Body ◽

Wilcoxon Test ◽

Control Group ◽

P Value ◽

Test Results ◽

Significant Difference ◽

Before And After

ABSTRACT Joint inflammation characterized by swelling of the joints, redness, heat, pain and movement disorders. Arthralgia causes the elderly to have difficulty in carrying out activities that are burdening the joints of the body. Wet cupping therapy is a complementary therapy that has a working principle of vacuum, injury and blood-sucking in certain areas so it can cure the disease. This study was to identify the effect of cupping therapy on pain intensity in elderly patients with arthralgia in Bangsalsari Jember. It was a quasy experiment research with pretest-posttest with control group approach. The sample in this study was 30 elderly then divided into two groups, i.e. 15 elderly in the wet cupping therapy group and 15 elderly in the warm compress group. It used purposive sampling. The average pain intensity before moist cupping therapy was 7.47, and after wet cupping therapy 5.53. While the moderate pain intensity before warm compress was 7.00 and after warm compress 5.73. Wilcoxon test results moist cupping therapy obtained P-value 0.001 means there are significant differences before and after the wet cupping therapy. While Wilcoxon test results, warm compress got P-value 0.001 means there are substantial differences before and after the warm compress. Mann Whitney test result obtained P-value 0.383 means there is no significant difference of effect between the treatment group and the control group. There is an effect of cupping therapy and warm compress on pain intensity. Elderly can use wet cupping therapy as an alternative treatment to decrease joint pain intensity arthralgia.

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