Hydrothermal fabrication of pure ZnSe nanocrystals at different microwave irradiation times and their disc-diffusion antibacterial potential against Gram negative bacteria: Bio-optical advantages

2021 ◽  
Vol 123 ◽  
pp. 108345
Author(s):  
Dariush Souri ◽  
Noushin Salimi ◽  
Mehdi Ghabooli
Keyword(s):  
Microwave Irradiation ◽  
Gram Negative Bacteria ◽  
Disc Diffusion ◽  
Gram Negative ◽  
Znse Nanocrystals ◽  
Antibacterial Potential

In-vitro Antioxidant and Antibacterial Activity of Methanolic Extract of Shorea robusta Gaertn. F. Resin

2016 ◽  
Vol 6 (4) ◽  
pp. 68
Author(s):  
Sushma Vashisht ◽  
Manish Pal Singh ◽  
Viney Chawla
Keyword(s):  
Antibacterial Activity ◽  
Methanolic Extract ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Disc Diffusion ◽  
Gram Positive ◽  
Gram Negative ◽  
Shorea Robusta ◽  
Dose Dependent ◽  
Resin Extract

The methanolic extract of the resin of Shorea robusta was subjected to investigate its antioxidant and antibacterial properties its utility in free radical mediated diseases including diabetic, cardiovascular, cancer etc. The methanol extract of the resin was tested for antioxidant activity using scavenging activity of DPPH (1,1-diphenyl-2-picrylhydrazil) radical method, reducing power by FeCl3 and antibacterial activity against gram positive and gram negative bacteria using disc diffusion method. The phytochemical screening considered the presence of triterpenoids, tannins and flavoniods. Overall, the plant extract is a source of natural antioxidants which might be helpful in preventing the progress of various oxidative stress mediated diseases including aging. The half inhibition concentration (IC50) of resin extract of Shorea robusta and ascorbic acid were 35.60 µg/ml and 31.91 µg/ml respectively. The resin extract exhibit a significant dose dependent inhibition of DPPH activity. Antibacterial activity was observed against gram positive and gram negative bacteria in dose dependent manner.Key Words: Shorea robusta, antioxidant, antibacterial, Disc-diffusion, DPPH.

scholarly journals Enaminone-Derived Pyrazoles with Antimicrobial Activity

2019 ◽  
Vol 2019 ◽  
pp. 1-10
Author(s):  
Mashooq Ahmad Bhat ◽  
Mohamed A. Al-Omar ◽  
Ahmed M. Naglah ◽  
Abdul Arif Khan
Keyword(s):  
Antimicrobial Activity ◽  
Gram Negative Bacteria ◽  
Significant Activity ◽  
Disc Diffusion ◽  
Initial Screening ◽  
Gram Positive ◽  
Gram Negative ◽  
Disc Diffusion Assay ◽  
Gram Positive Bacteria ◽  
Diffusion Assay

A series of pyrazoles derived from the substituted enaminones were synthesized and were evaluated for antimicrobial activity. All the compounds were characterized by the spectral data and elemental analysis. The synthesized compounds were initially screened for their antimicrobial activity against ATCC 6538, NCTC 10400, NCTC 10418, and ATCC 27853. During initial screening, compounds (P1, P6, and P11) presented significant antimicrobial activity through disc diffusion assay. These compounds were further evaluated for antimicrobial activity at different time points against Gram-positive and Gram-negative bacteria and presented significant activity for 6 hours. The activity was found to be greater against Gram-positive bacteria. In contrast at 24 hours, the activity was found only against Gram-positive bacteria except compound (P11), showing activity against both types of bacteria. Compound (P11) was found to have highest activity against both Gram-positive and Gram-negative bacteria.

scholarly journals “ISOLATION AND CHARACTERIZATION OF LACTOSE AND NON- LACTOSE FERMENTING BACTERIA FROM TERTIARY CARE HOSPITAL AND THEIR ANTIMICROBIAL SUSCEPTIBILITY TEST”

2017 ◽  
Vol 10 (2) ◽  
pp. 201
Author(s):  
Kumud Bala ◽  
Ridhima Wadhwa ◽  
Rachana Bohra
Keyword(s):  
Tertiary Care Hospital ◽  
Tertiary Care ◽  
Susceptibility Test ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Care Hospital ◽  
Disc Diffusion ◽  
Gram Negative ◽  
Fermenting Bacteria ◽  
Vitek 2

Objective: The purpose of the present study was to identify the fermenting and non-fermenting gram negative bacteria from the tertiary care hospital.Methods: The conventional method of identification by biochemical analysis and antibiotic susceptibility test was performed by Kirby-Bauer disc diffusion method. Furthermore, analysis of microbes was done by Vitek-2.Results: 424strains of lactose fermenting and non-lactose fermenting gram negative bacilli were isolated from 3097 clinical samples. From the total lactose fermenting bacteria Escherichia coli was the predominant isolate accounting for 50.94% specimens, followed by Klebsiella pneumonia 27.59% and Enterobacter 0.47%. From the total non-lactose fermenting gram negative bacilli Acinetobacter baumannii was the predominant isolate accounting for 12.73% specimens followed by Pseudomonas aeroginosa 6.13%, other isolates were Stenotrophomonas maltophilia 1.17% , Burkholderia cepacia 0.94%. In the present study male were more infected than female. The study also showed that lactose fermenting bacteria were more infectious than non lactose-fermenting bacteria and isolates were from urine samples.Conclusion: Both Non-Lactose Fermenting Gram Negative Bacilli and Lactose Fermenting Gram Negative Bacilli were found to be major contaminants, and are important pathogenic bacteria causing wide range of infections in the tertiary care hospital.Keywords: Lactose fermenting gram negative bacteria, Vitek-2, Tertiary Care Hospital, Kirby-Bauer Disc Diffusion, Lactose non-fermenting gram negative bacteria  

scholarly journals A Study on Antibacterial Activity of Silver Nanoparticles

2018 ◽  
Vol 29 (2) ◽  
pp. 37-41
Author(s):  
M Iqbal Hossain ◽  
M Anwar Habib ◽  
Nazimuddin Ahmed
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Antibacterial Effect ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Reference Drug ◽  
Gram Negative ◽  
Quasi Experimental

This quasi experimental study was designed to evaluate antibacterial activity of chemically synthesized silver nanoparticles (AgNPs) from silver nitrate (AgNO3) solutions on gram negative bacteria like E.Coli using disc diffusion method. Different concentrations of AgNPs, AgNO3 and reference drug ciprofloxacin were used to find out the antibacterial activity which revealed that AgNPs possessed significant antibacterial effect compared to AgNO3 solutions but relatively less antibacterial effect than that of ciprofloxacin. So chemical synthesis guided AgNPs may have some antibacterial effects.TAJ 2016; 29(2): 37-41

scholarly journals Synthesis and Antimicrobial Assessment of Fe3+ Inclusion Complex of p-tert-Butylcalix[4]arene Diamide Derivative

2019 ◽  
Vol 2019 ◽  
pp. 1-8 ◽  
Author(s):  
Anwar Ali Chandio ◽  
Ayaz Ali Memon ◽  
Shahabuddin Memon ◽  
Fakhar N. Memon ◽  
Qadeer Khan Panhwar ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Gram Negative ◽  
Mueller Hinton ◽  
Mueller Hinton Agar ◽  
Ft Ir ◽  
Bacteria And Fungi

Present study deals with the synthesis of the p-tert-butylcalix[4]arene diamide derivative as ligand (L) and its Fe3+ complex, followed by its characterization using TLC and FT-IR, while UV-Vis and Job’s plot study were performed for complex formation. Antimicrobial activity of the derivative (L) and its metal complex was carried out by the disc diffusion method against bacteria (Escherichia coli and Staphylococcus albus) and fungi (R. stolonifer). Different concentrations of the derivative (L) (6, 3, 1.5, 0.75, and 0.37 μg/mL) and its Fe3+ complex were prepared, and Mueller–Hinton agar was used as the medium for the growth of microorganisms. Six successive dilutions of the derivative (L) and Fe3+ complex were used against microorganisms. Two successive dilutions (6 and 3 μg/mL) of the derivative (L) showed antibacterial action against both Gram-positive and Gram-negative bacteria. In addition, three successive dilutions (6, 3, and 1.5 μg/mL) of the derivative (L) showed antifungal activity. However, all of six dilutions of the Fe3+ complex showed antimicrobial activity. Derivative (L) showed 3 and 1.5 μg/mL minimum inhibitory concentrations (MIC) against bacteria and fungi, respectively. On the contrary, its Fe3+ complex showed 0.37 μg/mL value of MIC against bacteria and fungi. Hence, Fe3+ complex of the derivative (L) was found to be a more effective antimicrobial agent against selected bacteria and fungi than the diamide derivative (L).

scholarly journals New Diarylheptanoid from Garuga pinnata Roxb

2013 ◽  
Vol 61 (2) ◽  
pp. 131-134 ◽  
Author(s):  
Mst Taslima Khatun ◽  
M. Mahboob Ali Siddiqi ◽  
Al-Mansur MA ◽  
MH Sohrab ◽  
AFM Mustafizur Rahman ◽  
...  
Keyword(s):  
Brine Shrimp ◽  
Microbial Growth ◽  
Stem Bark ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Gram Negative ◽  
Wide Range ◽  
Bacteria And Fungi

9´-Desmethylgaruganin I has been isolated from the dichloromethane extract of the stem bark of Garuga pinnata Roxb. The crude extract was screened for antimicrobial activity against a wide range of gram-positive and gram-negative bacteria and fungi by disc diffusion method and cytotoxicity by brine shrimp lethality bioassay. The dichloromethane extract showed moderate inhibitory activity to microbial growth and weak cytotoxicity having LC90 25.703?g-mL–1 DOI: http://dx.doi.org/10.3329/dujs.v61i2.17058 Dhaka Univ. J. Sci. 61(2): 131-134, 2013 (July)

scholarly journals Gölbaşı ve Azaplı Göllerinden (Adıyaman) İzole Edilen Bakterilerin Tiplendirilmesi ve Çoklu Antibiyotik Dirençliliklerinin Araştırılması

2017 ◽  
Vol 5 (1) ◽  
pp. 43
Author(s):  
Fikret Büyükkaya Kayış ◽  
Sadık Dinçer ◽  
Fatih Matyar ◽  
Hatice Aysun Mercimek Takcı ◽  
Melis Sümengen Özdenefe ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Seasonal Changes ◽  
Reference Value ◽  
Summer Season ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Disc Diffusion ◽  
Multiple Antibiotic Resistance ◽  
Disc Diffusion Method ◽  
Gram Negative

Identification and multiple antibiotic resistances of amphicillin, chloramphenicol, streptomycin and tetracycline resistant gram-negative bacteria that isolated microorganisms from Gölbaşı and Azaplı lakes (Adiyaman) were investigated in this study. Seasonally taken isolates of totally 386 bacteria in 10 different species from 7 genera were scanned against 16 antibiotics [gentamycin, imipenem, kanamycin, chloramphenicol, meropenem, nalidixic acid, nitrofurantoin, penicillin, cephalothin, cefazolin, cefpirome, ceftizoxime, cefuroxime, streptomycin, tetracycline and trimethoprim-sulphamethoxazole (Bioanalyse)] by using the disc diffusion method to determine the prevalence of multiple antibiotic resistance. Multiple antibiotic resistance of stations showed seasonal changes between 0.29 and 0.91. In generally, multiple antibiotic resistance in Golbasi and Azapli lakes were higher than the reference value and highest multiple antibiotic resistance values were obtained at summer season (3th period). When the obtained data are considered, high multiple antibiotic resistance poses a risk in terms of public health and for economically important animals.

scholarly journals Pharmacotherapeutic Activity of Allium sativum (Garlic) Bulb against Gram-positive and Gram-negative Bacteria

2020 ◽  
pp. 22-27
Author(s):  
Augustine I. Airaodion ◽  
Ada C. Ngwogu ◽  
Kenneth O. Ngwogu ◽  
John A. Ekenjoku ◽  
Anthony U. Megwas
Keyword(s):  
Inhibitory Activity ◽  
Allium Sativum ◽  
Diffusion Method ◽  
Local Market ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative ◽  
Garlic Bulb ◽  
Zones Of Inhibition ◽  
Antibacterial Potential

Background: Emergence of methicillin drug resistance is evident and has become a global challenge. Seeking for alternative antibiotics that are new, natural, plant based, cost effective and less toxic is the recent task for global health. Aim: This study is aimed at assessing the pharmacotherapeutic activity of Allium sativum (Garlic) bulb against gram-positive and gram-negative bacteria. Materials and Methods: Fresh A. sativum bulbs were purchased from a local market in Ibadan, Nigeria and were identified by a botanist. They were cut into small pieces and mashed in a laboratory with a mortar and pestle and the fluid squeezed out of the resultant slurry. The antibacterial activity of the juice was determined by diffusion method. Nutrient agar medium was prepared using standard method. Pure cultures of Coliform bacillus, Staphylococcus epidermidis, Streptococcus viridians, Salmonella typhi and Escherichia coli were obtained from the Department of Veterinary Microbiology and Parasitology, Federal University of Agriculture, Abeokuta, Nigeria. The juices were serially diluted to obtain 1.0%, 0.5%, 0.25% and 0.125% solutions in sterile test tubes. Sterilized 9 mm filter paper disc soaked in the diluted juice were placed on the plate and incubated for 24 hours at room temperature. The plates were examined for clear zones of inhibition. Presence of zones of inhibition indicated activity. Results: The results showed that A. sativum bulb has antibacterial potential against all the bacteria used in this study and also exhibited inhibitory activity against them. Conclusion: The result of this present study showed that A. sativum juice has high range of antibacterial potential against both gram positive (S. epidemidis and S. viridans) and gram negative bacteria (C. bacillus, E. coli and S. typhi). However, the extract has a greater inhibitory activity against gram positive bacteria than gram negative bacteria.

scholarly journals Synthesis, Characterization and Antimicrobial Activities of Co (III) and Fe (III) Complexes of Imino-N-heterocyclic Carbenes Ligands

2021 ◽  
pp. 59-67
Author(s):  
Samaila Abubakar ◽  
Musa Muktari ◽  
Rejoice Atiko
Keyword(s):  
Escherichia Coli ◽  
Salmonella Typhi ◽  
Antimicrobial Activities ◽  
Heterocyclic Carbenes ◽  
Gram Negative Bacteria ◽  
Disc Diffusion ◽  
Gram Negative ◽  
E Coli ◽  
Gram Positive Bacteria

The synthesis and antimicrobial application of Co (III) and Fe (III) complexes of imine functionalized N-heterocyclic carbene (Imino-NHC) ligands is reported. The ligand precursors 1-(2-[(hydroxyl-benzylidene)-amino]-ethyl)-3-R-3H-imidazol-1-ium bromide where R = pyridyl (1a) and benzyl (1b) have been reported in our previous work. The in-situ generated ligands of 1a and 1b have been successfully coordinated to CoBr2 and [FeI(Cp)(CO)2] leading to the isolation of air-stable N^C^N^O four coordinate Co(III)  complex 2 and a six-coordinate Fe(III) complex 3. The synthesised complexes were both found to be NMR inactive hence were characterize using FTIR and LRMS. The complexes were screened for antimicrobial activities against four gram-negative bacteria Escherichia Coli (E-coli), Shigella, Klebsiella pneumoniae (K. Pneumoniae) and Salmonella typhi (S. typhi) and a gram positive bacteria Staphylocossus aureus (S. aureus). The antimicrobial test was conducted using disc diffusion methods and based on the concentrations of 100, 200, 300, 400 and 500 µg/ mL, significant activities were recorded for both cobalt and the iron complexes.

scholarly journals A Mini Review of Antibacterial Properties of ZnO Nanoparticles

2021 ◽  
Vol 9 ◽  
Author(s):  
Sergey V. Gudkov ◽  
Dmitriy E. Burmistrov ◽  
Dmitriy A. Serov ◽  
Maxim B. Rebezov ◽  
Anastasia A. Semenova ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Zinc Oxide Nanoparticles ◽  
Antibacterial Effect ◽  
Antibacterial Properties ◽  
World Health ◽  
Oxide Nanoparticles ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
E Coli ◽  
Antibacterial Potential

The development of antibiotic resistance of bacteria is one of the most pressing problems in world health care. One of the promising ways to overcome microbial resistance to antibiotics is the use of metal nanoparticles and their oxides. In particular, numerous studies have shown the high antibacterial potential of zinc oxide nanoparticles (ZnO-NP) in relation to gram-positive and gram-negative bacteria. This mini-review includes an analysis of the results of studies in recent years aimed at studying the antibacterial activity of nanoparticles based on zinc oxide. The dependence of the antibacterial effect on the size of the applied nanoparticles in relation to E. coli and S. aureus is given. The influence of various ways of synthesis of zinc oxide nanoparticles and the main types of modifications of NP-ZnO to increase the antibacterial efficiency are also considered.

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