Evaluation of secondary metabolites of Hirsutella citriformis against Udaspes folus infecting Curcuma longa L.

2013 ◽  
Vol 7 (1) ◽  
pp. 7-14
Author(s):  
Arutselvi Ramachandran ◽  
Bala Saravanan Thangappan ◽  
Ponmurugan Ponnusamy
Keyword(s):  
Secondary Metabolites ◽  
Curcuma Longa ◽  
Hirsutella Citriformis

scholarly journals Phytochemical Characterization, Hepatoprotective Activity on Alcohol-Induced Toxicity of the Aqueous Extract of Curcuma longa (Zingiberaceae) in Wistar Rats

2021 ◽  
pp. 134-149
Author(s):  
Ameaka Fatima Nkempu ◽  
Tembe Estella ◽  
Tchadji Mayoudom Vanessa Edwige ◽  
Bayaga Herve ◽  
Dobgima John Fonmboh ◽  
...  
Keyword(s):  
Liver Disease ◽  
Secondary Metabolites ◽  
Aqueous Extract ◽  
Wistar Rats ◽  
Liver Toxicity ◽  
Curcuma Longa ◽  
Hepatoprotective Activity ◽  
Bioactive Metabolites ◽  
Central Vein ◽  
Phytochemical Screening

Introduction: Liver or hepatic disease refers to different conditions that affect the liver. Chronic alcohol consumption is one of the most frequent causes of liver disease and accounts for about 55% of liver cirrhosis deaths recorded in Cameroon in 2020. Standard accessible treatments focus on end-stage liver disease with safety and efficacy obstacles. We have a research gap in Cameroon to understand the alternative use of natural products as treatment with a long traditional history of safe use. Curcuma longa has long been a source of traditional and modern medicine. It is commonly used in Cameroon as a spice and herbal product with some level of activity against various forms of liver disease. Objective: To phytochemically screen for bioactive metabolites and evaluate the hepatoprotective activity of the aqueous extract of Curcuma longaon alcohol-induced toxicity in Wistar rats. Methods: Phytochemical screening was carried out on the aqueous extract obtainedfrom maceration of plant rhizomes. Three doses (125, 250 and 500mg/Kg) of the plant extract and the reference (Silymarin 50mg/Kg) were administered daily (p.o) to rats 30 min before administration of 40% alcohol (2mL/100g p.o) for 21 days. Biochemical parameters such as ALAT, ASAT, GGT, Bilirubin and Lipid profile were quantified and histological studies of the liverwas carried out using standard procedures. Results: Phytochemical screening of the aqueous extract of C. longa revealed polyphenols such as flavonoids, tannins, quinones, saponins and phlobatanins. The plant showed hepatoprotective activity by decreasing liver toxicity markers such as ASAT, ALAT, GGT and Bilirubin. Histology revealed dose-dependent protection with 500 mg/Kg showing the most cellular integrity, no central vein occlusion and minimal fibrosis. Conclusion: This study indicated the presence of polyphenols like flavonoids and tannins in the aqueous extract of C. longa. The presence of these secondary metabolites in the studied extract justifies its antioxidant and anti-inflammatory properties confirmed by its hepatoprotective effects on alcohol-induced toxicity. This was clearly shown by biochemical and histological parameters. More sensitive and specific methods are required to test for these secondary metabolites in serum.

scholarly journals Evaluation of the Systemic Serum Exposure and Acute Toxicity of the Aqueous Extract of Curcuma longa (Zingiberaceae) Rhizomes in Wistar Rats

2021 ◽  
pp. 242-252
Author(s):  
Ameaka Fatima Nkempu ◽  
Tembe Estella Fokunang ◽  
Bayaga Hervé Narcisse ◽  
Eustace Bonghan Berinyuy ◽  
Tabi Yves Omgba ◽  
...  
Keyword(s):  
Body Weight ◽  
Secondary Metabolites ◽  
Acute Toxicity ◽  
Oral Dose ◽  
Aqueous Extract ◽  
Liver Damage ◽  
Biochemical Markers ◽  
Liver Toxicity ◽  
Curcuma Longa ◽  
Health Concern

Introduction: Liver toxicity has become a public health concern as more people globally get exposed to xenobiotics with the potential to cause liver damage and consequent liver cirrhosis. The increase in liver toxicant abuse has necessitated the exploration of xenobiotic exposure levels when addressing therapeutic measures using alternative herbal remedies. The increasing use of herbal products as alternative therapy needs regulatory alignment through evidence-based support for the safety and efficacy of these natural products. To undertake preclinical discovery of new metabolites from medicinal products, the objective of this study was to investigate the systemic serum exposure and acute toxicity of the aqueous extract of Curcuma longa (Zingiberaceae) rhizomes on Wistar rat models. Methods: Phytochemical screening was carried out on the aqueous extract obtained by maceration of the dried plant rhizomes. Standard screening techniques for plant metabolites were used to screen blood serum after animal exposure with the extract. After a 500mg/Kg dose, systemic exposure was evaluated in blood samples collected at 30-minute intervals for one hour. For acute toxicity, a single 2000mg/Kg by body weight dose of the plant extract and the reference (Silymarin 50mg/Kg) were administered to rats, and they were observed for 14 days. Biochemical markers of toxicity such as ALAT, ASAT, GGT, Bilirubin were quantified, and histological studies of the liver were carried out. Results: No secondary metabolites were identified at 30 mins and 1hr in rat serum following a 500 mg/Kg oral dose. Administration of a 2000 mg/Kg oral dose to rats was well tolerated, and there were no deaths or significant target organ toxicity. The plant showed no lethality at the dose of 2000mg/kg body weight and decreased liver toxicity markers such as ASAT, ALAT, GGT, and Bilirubin. Histology revealed no significant damage to liver hepatocytes, no central vein occlusion, and no evidence of fibrosis. Conclusion: There were no systemically available secondary metabolites at a dose of 500 mg/Kg after the qualitative screening; more sensitive and specific methods are required to test these secondary metabolites in serum. This study confirmed the safety margin of Curcuma longa with no lethality following a single oral dose of 2000mg/Kg and after observation for 14 days. There was a low expression of biochemical markers of toxicity ALAT, ASAT, and no histological indication of liver damage.

scholarly journals MORFOLOGI, ANATOMI DAN UJI HISTOKIMIA RIMPANG Curcuma aeruginosa Roxb; Curcuma longa L. DAN Curcuma heyneana Valeton dan Zijp.

2018 ◽  
Vol 17 (2) ◽  
Author(s):  
Trimanto Trimanto ◽  
Dini Dwiyanti ◽  
Serafinah Indriyani
Keyword(s):  
Secondary Metabolites ◽  
Traditional Medicine ◽  
Cell Density ◽  
Secretory Cell ◽  
Curcuma Longa ◽  
Anatomical Structure ◽  
Medium Size ◽  
Anatomical Structures ◽  
Primary And Secondary Metabolites ◽  
Histochemical Test

Curcuma is a genus of family Zingiberaceae. Its rhizomes, as well as leaves, have long been used in the traditional medicine. This research aimed to examine the morphological and anatomical structure as well as the primary and secondary metabolites of Curcuma aeruginosa Roxb, Curcuma longa L, and Curcuma heyneana Valeton & Zijp. The Anatomical structure, histochemical test and secretory cell density were observed microscopically. The Histochemical test consisted of amilum, protein, lipid, tanin, alkaloid dan flavonoid tests. Observation of anatomical structures of the of rhizome showed that starch grains has a medium size and shape of starch was oval. Rhizomes of Curcuma longa and C. aeruginosa had a positive correlation for starch, protein, lipids, alkaloids, flavonoids and tannins. C. heyneana has the highest density value on protein while C. longa has the highest density value on lipids, alkaloids, flavonoids and tannins.

scholarly journals Variable Secondary Metabolite Profiles Across Cultivars of Curcuma longa L. and C. aromatica Salisb.

2021 ◽  
Vol 12 ◽  
Author(s):  
Poonam Kulyal ◽  
Satyabrata Acharya ◽  
Aditya B. Ankari ◽  
Praveen K. Kokkiripati ◽  
Sarada D. Tetali ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Essential Oil ◽  
Essential Oils ◽  
Biological Activities ◽  
Curcuma Longa ◽  
Methanolic Extracts ◽  
Cosmetic Industry ◽  
Metabolite Profiles ◽  
New Compounds ◽  
Phytochemical Profiles

Background:Curcuma spp. (Zingiberaceae) are used as a spice and coloring agent. Their rhizomes and essential oils are known for medicinal properties, besides their use in the flavoring and cosmetic industry. Most of these biological activities were attributed to volatile and nonvolatile secondary metabolites present in the rhizomes of Curcuma spp. The metabolite variations among the species and even cultivars need to be established for optimized use of Curcuma spp.Objectives: We compared the phytochemical profiles of rhizomes and their essential oils to establish the variability among seven cultivars: five of Curcuma longa L. (Alleppey Supreme, Duggirala Red, Prathibha, Salem, Suguna) and two of C. aromatica Salisb. (Kasturi Araku, Kasturi Avidi). The GC-MS and LC-MS-based analyses were employed to profile secondary metabolites of these selected cultivars.Methods: Rhizomes of Curcuma spp. were subjected to hydro-distillation to collect essential oil and analyzed by GC-MS. The methanol extracts of fresh rhizomes were subjected to LC-MS analyses. The compounds were identified by using the relevant MS library databases as many compounds as possible.Results: The essential oil content of the cultivars was in the range of 0.74–1.62%. Several compounds were detected from the essential oils and rhizome extracts by GC-MS and LC-MS, respectively. Of these, 28 compounds (13 from GCMS and 15 from LCMS) were common in all seven cultivars, e.g., α-thujene, and diarylheptanoids like curcumin. Furthermore, a total of 39 new compounds were identified from C. longa L. and/or C. aromatica Salisb., most of them being cultivar-specific. Of these compounds, 35 were detected by GC-MS analyses of essential oils, 1,2-cyclohexanediol, 1-methyl-4-(1-methylethyl)-, and santolina alcohol, to name a few. The other four compounds were detected by LC-MS of the methanolic extracts of the rhizomes, e.g., kaempferol-3,7-O-dimethyl ether and 5,7,8-trihydroxy-2′,5′-dimethoxy-3′,4′-methylene dioxyisoflavanone.Conclusions: We identified and recorded the variability in the metabolite profiles of essential oils and whole rhizome extracts from the seven cultivars of Curcuma longa L. and C. aromatica Salisb. As many as 39 new metabolites were detected in these seven Indian cultivars of Curcuma spp. Many of these compounds have health benefits.

scholarly journals Antimicrobial Activities of Some Indonesian Medicinal Plants against Propionibacterium acnes (ATCC 27853) and Staphylococcus epidermis (ATCC 12228) causing Acne

2019 ◽  
Vol 32 (1) ◽  
pp. 41-44
Author(s):  
Ida Duma Riris ◽  
Albinus Silalahi ◽  
Tita Juwitaningsih ◽  
Wesly Hutabarat
Keyword(s):  
Medicinal Plants ◽  
Secondary Metabolites ◽  
Propionibacterium Acnes ◽  
Curcuma Longa ◽  
Antibacterial Activities ◽  
Psidium Guajava ◽  
Antimicrobial Activities ◽  
Lawsonia Intracellularis ◽  
Staphylococcus Epidermis ◽  
Local Plants

Secondary metabolites investigation of local plants, such as Psidium guajava L, Curcuma longa rhizome, Citrus hystrix and Lawsonia intracellularis shown that they have antibacterial activities against Propionibacterium acnes (ATCC 27853) and Staphylococcus epidermis (ATCC 12228) with chloramphenicol as antibiotic controlled. The phytoscreening method used to find out compounds in the plants and inhibition tests carried out by diffuse test with a concentration variation of 10, 5, 2.5 and 1.25 %. It was found that Lawsonia intracellularis plants has the highest inhibitory growth of 17; 12, 15, 14, 12 mm at 10 % concentration.

Secondary Metabolites, Pharmacognostic and Therapeutic Activities of the Rhizome Extract of Curcuma longa Grown in South-West, Nigeria

2020 ◽  
Vol 9 (1) ◽  
pp. 30-37
Author(s):  
Ololade Zaccchaeus S ◽  
◽  
Abam Esther O ◽  
Anuoluwa Iyadunni A ◽  
Abiona Oluwafunmilayo O ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Curcuma Longa ◽  
South West ◽  
Therapeutic Activities

scholarly journals Metabolite Profiling of Three Curcuma Species (Zingiberaceae) Based on H-NMR Spectroscopy

2019 ◽  
Vol 15 (1) ◽  
pp. 79
Author(s):  
Dinar Sari Cahyaningrum Wahyuni ◽  
Mutya Puti Wardianti ◽  
Yudi Rinanto ◽  
Soerya Dewi Marliyana
Keyword(s):  
Nmr Spectroscopy ◽  
Secondary Metabolites ◽  
Curcuma Longa ◽  
Quantitative Characteristic ◽  
Principal Component ◽  
Proton Nuclear Magnetic Resonance ◽  
Tropical Region ◽  
Anticancer Activities ◽  
Primary And Secondary Metabolites ◽  
H Nmr

Genus <em>Curcuma</em>, Zingiberaceae, is a typical medicinal plant in tropical region especially in Indonesia. It has been studied to have antioxidant, antimicrobial, antitumor, anti-inflammatory and anticancer activities. However, little knowledge of the metabolic profile both primary and secondary metabolites have been reported. Thus, this study aims to investigate metabolic profiling both primary and secondary metabolites simultaneously in the <em>Curcuma</em> species based on proton nuclear magnetic resonance (<sup>1</sup>H-NMR) spectroscopy. The present work applied metabolomic study which measured the qualitative and quantitative characteristic metabolites. The <em>Curcuma</em> species, <em>Curcuma aeruginosa </em>Roxb., <em>Curcuma xanthorrhiza </em>Roxb., and <em>Curcuma longa </em>L., collected from Nguter, Sukoharjo, Indonesia. Two-dimensional (2D)-NMR techniques were applied to further identify a number of different types of compounds. Multivariate data analysis such as Principal Component Analysis (PCA) applied to reveal differences among species. A clear difference occurred among 3 Curcuma species. Primary metabolites responsible for the discrimination are alanine (<em>C. xanthorrhiza</em> Roxb. was 3.78 times higher than in <em>C. longa </em>L), sugars (<em>C. xanthorrhiza</em> Roxb. were 6.03 and 3.81 times higher in <em>C. aeruginosa </em>Roxb. and <em>C. longa </em>L. respectively). Besides, secondary metabolites which differed among 3 species are curcumin (<em>C. xanthorrhiza </em>Roxb. were 38.25 and 25 times higher than in <em>C. aeruginosa</em> Roxb.) and xanthorrhizol (<em>C. longa </em>L. were 62 and 44.4 times higher than in <em>C aeruginosa </em>Roxb.).

Lycopene alleviates sulfamethoxazole-induced hepatotoxicity in grass carp (Ctenopharyngodon idellus) via suppression of oxidative stress, inflammation and apoptosis

2020 ◽  
Vol 11 (10) ◽  
pp. 8547-8559
Author(s):  
Hongjing Zhao ◽  
Yu Wang ◽  
Mengyao Mu ◽  
Menghao Guo ◽  
Hongxian Yu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Secondary Metabolites ◽  
Human Health ◽  
Grass Carp ◽  
Aquatic Environment ◽  
Ctenopharyngodon Idellus

Antibiotics are used worldwide to treat diseases in humans and other animals; most of them and their secondary metabolites are discharged into the aquatic environment, posing a serious threat to human health.

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