Hepatoprotective effect of grape seed oil against carbon tetrachloride induced oxidative stress in liver of γ-irradiated rat

2016 ◽  
Vol 160 ◽  
pp. 1-10 ◽  
Author(s):  
Amel F.M. Ismail ◽  
Asmaa A.M. Salem ◽  
Mamdouh M.T. Eassawy
Keyword(s):  
Oxidative Stress ◽  
Carbon Tetrachloride ◽  
Seed Oil ◽  
Grape Seed ◽  
Hepatoprotective Effect ◽  
Grape Seed Oil

scholarly journals Effect of Grape Seed Oil on Chronic Carbon Tetrachloride-Induced Hepatic Injury and Determination of Hepatic Apoptosis in Rats

2019 ◽  
pp. 1-10
Author(s):  
Ayhan Atasever ◽  
Ahmet Alpay ◽  
Gorkem Ekebas ◽  
Duygu Yaman Gram
Keyword(s):  
Carbon Tetrachloride ◽  
Hepatic Injury ◽  
Caspase 3 ◽  
Seed Oil ◽  
Antioxidant Properties ◽  
Elevated Serum ◽  
Grape Seed ◽  
Control Group ◽  
Albino Rats ◽  
Grape Seed Oil

Aims: The present study was designed to evaluate the hepatoprotective activity of grape seed oil (GSO) on liver lesions induced by carbon tetrachloride (CCl4) in rats. The effects on hepatic injury were investigated by measuring serum levels of ALT, triglyceride, total protein, total cholesterol and liver levels of MDA. Furthermore, caspase -3, -8 and -9 activities in cellular apoptosis were determined. Place and Duration of Study: Faculty of Veterinary Medicine, Department of Pathology, Erciyes University, Kayseri, between November 2017 and September 2018. Methodology: In this study 40 male Wistar albino rats were divided into four groups including 10 animals in each. Control group administered with 0.9% NaCl. The second group was administered with 4 mL/kg GSO for twelve weeks. Third group were given CCl4 (0.2 mL/kg) twice for 8-weeks. Fourth group was administered with 4 mL/kg GSO, for 12 weeks and also given CCl4 (0.2 mL/kg) twice for 8 weeks, starting from the 5th week. Results: Histopathological examination of CCl4 group showed intense macro and micro vesicular steatosis in hepatocytes, necrosis, and lymphocytes rich mononuclear cell infiltration in portal area and mild portal fibrosis in the parenchyma. The grape seed oil applications have partially normalized the altered histological changes and the activity of caspase -3, -8 and -9. Administration of GSO led to a decline in the activities of ALT and MDA levels while this treatment elevated serum triglycerides levels which are not significantly important. Conclusion: The results indicate that the antioxidant properties of GSO have not ameliorative effect in either the histopathological lesions or biochemical parameters against CCl4-induced hepatotoxicity in rats. Also, it has been concluded that duration‐dependent further research results are needed to determine the effects of grape seed oil in high doses which can give the best results without side effects.

scholarly journals AMELIORATIVE EFFECTS OF GRAPE SEED OIL ON CHROMIUM-INDUCED NEPHROTOXICITY AND OXIDATIVESTRESS IN RATS

2020 ◽  
Vol 57 (3) ◽  
Author(s):  
Sahar Hassan Orabi ◽  
Sherif Mohamed Shawky
Keyword(s):  
Dna Damage ◽  
Hexavalent Chromium ◽  
Seed Oil ◽  
Potassium Dichromate ◽  
Serum Levels ◽  
Grape Seed ◽  
Renal Tissue ◽  
Male Rats ◽  
Group I ◽  
Grape Seed Oil

The current study focused on investigating the renoprotective effects of grape seed oil (GSO) against hexavalent chromium (Cr (VI))-induced nephrotoxicity. A total of 40 male rats were randomly divided into four groups: group I served as the control group, group II received 1000 mg/L potassium dichromate (353.5 mg/L Cr(VI)) in drinking water for 12 weeks, group III received 3.7 g/kg body weight/day GSO orally for 12 weeks, and group IV received GSO together with potassium dichromate for 12 weeks. Cr(VI) significantly increased serum levels of urea, creatinine, potassium and glucose. In addition, Cr(VI) increased MDA levels and induced renal tissue damage and DNA damage. On the other hand, Cr(VI) decreased serum levels of sodium and antioxidant defence system [reduced glutathione (GSH) and catalase (CAT)]. However, treatment with GSO prevented elevation levels of serum urea, creatinine, potassium and glucose. In addition, GSO enhanced sodium level, renal tissue antioxidant defense system due to its curative effect ameliorated particularly oxidative stress, renal tissue and DNA damage. In conclusion, these results demonstrate that GSO is a promising nephroprotective agent against Cr(VI)-induced nephrotoxicity.Key words: grape seed oil; hexavalent chromium; nephrotoxicity; DNA damage BLAŽILNI UČINKI OLJA GROZDNIH PEŠK PRI TOKSIČNI OBREMENITVI LEDVIC TER VPLIV NA OKSIDATIVNI STRES PODGAN, POVZROČEN S KROMOM Povzetek: Študija je bila osredotočena na proučevanje zaščitnih učinkov olja grozdnih pešk (GSO) pri toksični obremenitvi ledvic, povzročeni s heksavalentnim kromom (Cr (VI)). Štirideset samcev podgan je bilo naključno razdeljenih v štiri skupine: skupina I - kontrolna skupina, skupina II, ki je v pitni vodi 12 tednov prejemala 1000 mg/L kalijevega dikromata (353,5 mg/L Cr (VI)), skupina III, ki je peroralno 12 tednov prejemala 3,7 g/kg telesne mase/dan GSO ter skupina IV, ki je 12 tednov prejemala GSO skupaj s kalijevim dikromatom. Cr(VI) je znatno zvišal serumske ravni sečnine, kreatinina, kalija in glukoze v serumu. Poleg tega je Cr(VI) zvišal raven MDA in povzročil poškodbe ledvičnega tkiva in poškodbe DNK. Po drugi strani je Cr(VI) znižal serumsko raven natrija in antioksidativnega obrambnega sistema, zmanjšal raven glutationske peroksidaze in katalaze. Dodajanje GSO poskusnim živalim je preprečilo zvišanje ravni sečnine v serumu, kreatinina, kalija, natrija in glukoze. Poleg tega je GSO izboljšal obrambni sistem antioksidantov ledvičnega tkiva. Zaradi svojega zdravilnega učinka je izboljšal zlasti oksidativni stres, poškodbe ledvičnega tkiva in DNK. Rezultati kažejo, da je GSO obetavno zaščitno sredstvo za ledvica pri toksični obremenitvi, povzročeni s Cr(VI).Ključne besede: olje grozdnih pešk; heksavalentni krom; nefrotoksičnost; poškodba DNK

scholarly journals Characterization of fatty acid, antioxidant, and polyphenol content of grape seed oil from different Vitis vinifera L. varieties

OCL ◽  
2021 ◽  
Vol 28 ◽  
pp. 30
Author(s):  
Viktória Kapcsándi ◽  
Erika Hanczné Lakatos ◽  
Beatrix Sik ◽  
László Ádám Linka ◽  
Rita Székelyhidi
Keyword(s):  
Seed Oil ◽  
Grape Seed ◽  
Seed Oils ◽  
Grape Seeds ◽  
Polyphenol Content ◽  
Total Polyphenol ◽  
Total Polyphenol Content ◽  
Grape Seed Oil ◽  
Total Antioxidant ◽  
Grape Varieties

In this study, we examined the yield and oil quality of eight different grape varieties. For the experiments, the grape seeds were obtained from the Benedictine Pannonhalma Archabbey in the northwestern region of Hungary. The aim of the studies was to determine the oil yield obtained by extraction and to examine the differences between the fatty acid composition, antioxidant capacity, and total polyphenol content of the oils of different grape varieties. Based on the results, the oil content of the grape seeds varied between 99.91 g/kg and 126.74 g/kg. The grape seed oils analysed contained significant amounts of stearic acid (3.42–9.93%), palmitic acid (7.81–10.66%), oleic acid (14.29–19.92%), and linoleic acid (66.85–72.47%). Besides, the grape seed oils tested contained several other fatty acids in small amounts. There were significant differences in the total antioxidant and total polyphenol content of the oils. Total polyphenol content ranged from 0.24 to 1.13 mg GAE/g, while the total antioxidant content changed between 0.12 and 0.78 μg TEAC/g. The results show that the studied varieties are suitable for the production of table grape seed oil based on their oil yield, and the oils have favourable, health-protecting properties in terms of their quality.

scholarly journals Protective effects of vitamin E and grape seed oil against acute hepatorenal ivermectin toxicity in mice: biochemical and histopathological studies

2019 ◽  
Vol 7 (2) ◽  
pp. 087-094
Author(s):  
Mai Mohsen El Maghraby ◽  
Nada Mosaad El Maghraby ◽  
Ameera Ahmed Salama ◽  
Azza Shawky Abdlmonem ◽  
Eman Abdellatefe Authman ◽  
...  
Keyword(s):  
Vitamin E ◽  
Seed Oil ◽  
Grape Seed ◽  
Protective Effects ◽  
Grape Seed Oil ◽  
Histopathological Studies

scholarly journals Effect of Gold Sea Cucumber (Stichopus hermanni) Extract Concentration on Antioxidant Activity of Grape Seed Oil (Vitis vinifera) nanoemulsion

2018 ◽  
Vol 16 (1) ◽  
pp. 36 ◽  
Author(s):  
Siti Umroh Noor ◽  
Amelia Gozali
Keyword(s):  
Antioxidant Activity ◽  
Sea Cucumber ◽  
Seed Oil ◽  
Grape Seed ◽  
Omega 3 ◽  
Extract Concentration ◽  
Globule Size ◽  
Grape Seed Oil ◽  
Dpph Method ◽  
Antioxidant Agent

Gold sea cucumber extract (Stichopus hermanni) is rich in collagen and unsatturated fatty acid also known omega 3 such as docosahexaenoic acid and eicosapentaenoic acid that highly effective as an antioxidant. Oligomeric proanthocyanidin is the main content in grape seed oil and the most powerful antioxidant agent that can protect collagen. The aim of research was to determine the effect of gold sea cucumber extract concetration on antioxidant activity of grape seed oil nanoemulsion. The three formulas with results test of antioxidant activity with DPPH method using 15% Brij CS12® and 30% propylene glycol has done which the oil phase was dispersed in the aqueous phase.Variation that have been done is to use the antioxidant activity (IC50) of gold sea cucumber extract  for 2, 4, and 6 times. The resulting preparations was conformed of organoleptic, nanoemulsion type, clarity, globule size, viscosity and rheology, pH and freez thaw test. Antioxidant activity of gold sea cucumber extract and grape seed oil were  (IC50= 53.79 and 17.41 μg/mL respectively). The nanoemulsion was analysed using two-way ANOVA method. Antioxidant  activity at storage for 0; 2 and 4-weeks of formula I, II, III were (IC50=  49.15; 49.45, 51.15 µg/mL), (IC50= 44.12, 47.48, 48.14 µg/mL), and (IC50= 42.14, 43.45, 45.33 µg/mL) respectively. Can be concluded that the higher concentration of gold sea cucumber extract, the antioxidant activity of nanoemulsion is higher, but it decreased during the storage.

Chemical characterization and phase behaviour of grape seed oil in compressed carbon dioxide and ethanol as co-solvent

2010 ◽  
Vol 42 (6) ◽  
pp. 797-801 ◽  
Author(s):  
Irede Dalmolin ◽  
Marcio A. Mazutti ◽  
Eduardo A.C. Batista ◽  
M. Angela A. Meireles ◽  
J. Vladimir Oliveira
Keyword(s):  
Carbon Dioxide ◽  
Seed Oil ◽  
Chemical Characterization ◽  
Grape Seed ◽  
Phase Behaviour ◽  
Grape Seed Oil ◽  
Compressed Carbon Dioxide

scholarly journals Effect of drying method on supercritical CO2 extraction of grape seed oil

Poljoprivreda ◽  
2019 ◽  
Vol 25 (1) ◽  
pp. 81-88 ◽  
Author(s):  
Mario Jakobović ◽  
Stela Jokić ◽  
Melita Lončarić ◽  
Snježana Jakobović ◽  
Krunoslav Aladić ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Sample Preparation ◽  
Supercritical Co2 ◽  
Seed Oil ◽  
Grape Seed ◽  
Cabernet Sauvignon ◽  
Drying Method ◽  
Supercritical Co2 Extraction ◽  
White Variety ◽  
Grape Seed Oil

Aim of this study was to monitor the influence of drying method (naturally and chamber drying) and different sample preparation on supercritical CO2 extraction of oil from three grape seed varieties (Graševina, Zweigelt, Cabernet Sauvignon). The highest oil content was obtained from naturally dried screened and washed seeds of red variety Cabernet Sauvignon (14.85%) and lowest from chamber dried screened seeds of white variety Graševina (7.67%). Peroxide value ranged from 0.36 to 1.77 mmol O2/kg oil, free fatty acids 0.28-8.0%, and insoluble impurities 0.05-0.28%. Determined fatty acids were palmitic (6.98-11.58%), stearic (3.82-6.59%), oleic (14.90-19.97%) and linoleic acid (61.82-71.96%) in oil obtained from naturally dried seeds and 6.84-8.68%, 4.12-5.73%, 15.10-20.18% and 67.88-70.76% in oil from chamber dried seeds, respectively. In defatted cakes after supercritical CO2 extraction, protein and fibre content ranged from 8.17 to 9.85% and 34.58 to 43.96%, respectively. According to ANOVA results, sample preparation and drying method had statistically significant influence on grape seed oil extraction.

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