Change in expression levels of NAD kinase-encoding genes in Flaveria species

2021 ◽  
pp. 153495
Author(s):  
Masami Tanaka ◽  
Yuuma Ishikawa ◽  
Sayaka Suzuki ◽  
Takako Ogawa ◽  
Yukimi Y. Taniguchi ◽  
...  
Keyword(s):  
Nad Kinase ◽  
Expression Levels ◽  
Encoding Genes ◽  
Flaveria Species

scholarly journals DNA Methylation Influences miRNA Expression in Gonadotroph Pituitary Tumors

Life ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 59
Author(s):  
Joanna Boresowicz ◽  
Paulina Kober ◽  
Natalia Rusetska ◽  
Maria Maksymowicz ◽  
Agnieszka Paziewska ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Mirna Expression ◽  
Target Genes ◽  
Expression Profiles ◽  
Pituitary Tumors ◽  
Target Prediction ◽  
Aberrant Methylation ◽  
Expression Levels ◽  
Putative Target ◽  
Encoding Genes

microRNAs are involved in pathogenesis of cancer. DNA methylation plays a role in transcription of miRNA-encoding genes and may contribute to changed miRNA expression in tumors. This issue was not investigated in pituitary neuroendocrine tumors (PitNETs) previously. DNA methylation patterns, assessed with HumanMethylation450K arrays in 34 PitNETs and five normal pituitaries, were used to determine differentially methylated CpGs located at miRNA genes. It showed aberrant methylation in regions encoding for 131 miRNAs. DNA methylation data and matched miRNA expression profiles, determined with next-generation sequencing (NGS) of small RNAs, were correlated in 15 PitNETs. This showed relationship between methylation and expression levels for 12 miRNAs. DNA methylation and expression levels of three of them (MIR145, MIR21, and MIR184) were determined in the independent group of 80 tumors with pyrosequencing and qRT-PCR and results confirmed both aberrant methylation in PitNETs and correlation between methylation and expression. Additionally, in silico target prediction was combined with analysis of established miRNA profiles and matched mRNA expression pattern, assessed with amplicon-based NGS to indicate putative target genes of epigenetically deregulated miRNAs. This study reveals aberrant DNA methylation in miRNA-encoding genes in gonadotroph PitNETs. Methylation changes affect expression level of miRNAs that regulate putative target genes with tumorigenesis-relevant functions.

scholarly journals Effects of fosfomycin on Shiga toxin-producing Escherichia coli: quantification of copy numbers of Shiga toxin-encoding genes and their expression levels using real-time PCR

2009 ◽  
Vol 58 (7) ◽  
pp. 971-973 ◽  
Author(s):  
Naoko Ichinohe ◽  
Yuko Ohara-Nemoto ◽  
Takayuki K. Nemoto ◽  
Shigenobu Kimura ◽  
Sadato Ichinohe
Keyword(s):  
Escherichia Coli ◽  
Real Time ◽  
Shiga Toxin ◽  
Real Time Pcr ◽  
Expression Levels ◽  
Copy Numbers ◽  
Encoding Genes

scholarly journals Induksi Ekspresi Gen Sitokin/Kemokin pada Sel Makrofag Manusia yang Dipapar Virus Dengue Isolat Indonesia

2014 ◽  
Vol 1 (3) ◽  
pp. 146-157
Author(s):  
Siti Warnasih
Keyword(s):  
Dengue Virus ◽  
Mononuclear Cells ◽  
Expression Profiles ◽  
Gradient Centrifugation ◽  
Gene Expression Profiles ◽  
Host Gene ◽  
Peripheral Blood Mononuclear ◽  
Expression Levels ◽  
Dengue Disease ◽  
Encoding Genes

Dengue is one of the world's most important arbovirus disease. Dengue pathogenesis has not been yet fully understood. It has been reported that there is involvement of the host immune factors and viral factors. Several studies have shown that concentrations of cytokines/chemokines on blood are significantly increased during infection and viral factors are also involved in disease severity. Therefore, characterization of host gene expression profiles in response to dengue virus infection of different serotypes could provide input for understanding the pathogenesis of dengue. The purpose of this research was to determine expression profiles of the genes (mRNA) of cytokines/chemokines as immune response that are released by monocyte derived macrophages (MDM) cells (host gene) exposed dengue viruses. Four dengue serotypes of Indonesia isolate were used in this study. Peripheral Blood Mononuclear Cells (PBMCs) were isolated from blood cells of healthy donors by Ficoll gradient centrifugation techniques and then differentiated into MDM cells. Quantitative real time RT-PCR was used to quantify expression levels of cytokine/chemokine-encoding genes from MDM cells infected dengue. Four cytokine/chemokine-encoding genes i.e IP-10, MCP-1, IL-10, and MIP-1β known involved in dengue pathogenesis. Measurement of the expression levels of cytokines/ chemokines showed that the dengue virus of serotypes DENV-1 and DENV-3 caused an increase in the expression of genes encoding cytokine IL-10 and chemokine IP-10 is higher than other serotypes. Further research is needed to better determine the pathogenesis of dengue disease.

Gene expression analysis of metabolic markers during bone regeneration in a rat model

2014 ◽  
Vol 23 (03) ◽  
pp. 207-211
Author(s):  
C. Kasch ◽  
A. Osterberg ◽  
Thordis Granitzka ◽  
T. Lindner ◽  
M. Haenle ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Analysis ◽  
Saline Solution ◽  
Female Rats ◽  
Synthesis Rate ◽  
Metabolic Markers ◽  
Expression Levels ◽  
Fibrotic Tissue ◽  
Intermittent Pth ◽  
Lower Expression

SummaryThe RANK/RANKL/OPG system plays an important role in the regulation of bone metabolism and bony integration around implants. The aim of this study was to analyse gene expression of OPG, RANK, and RANKL in regenerating bone during implant integration. Additionally, the effect of intermittent para - thyroid hormone (PTH) treatment was analysed. A titanium chamber was implanted in the proximal tibiae of 48 female rats. The animals received either human PTH or saline solution (NaCl). After 21 and 42 days, RNA was isolated from tissue adjacent to the implant and expression of RANK, RANKL, and OPG was analysed. After 21 days, very low expression levels of all genes were shown. In contrast, increased gene expression after 42 days was determined. Expression of RANK and RANKL was lower than that for OPG. The lower expression levels after 21 days might be due to still ossifying, fibrotic tissue around the titanium chamber. An increased OPG synthesis rate associated with decreased RANKL expression after 42 days revealed bone-forming processes. Despite significant differences in gene expression between the time points, only slight differences were observed between application of intermittent PTH and NaCl after a period of 42 days.

High Level Expression of Active Human Prothrombin in a Vaccina Virus Expression System

1992 ◽  
Vol 68 (02) ◽  
pp. 119-124 ◽  
Author(s):  
F G Falkner ◽  
P L Turecek ◽  
R T A MacGillivray ◽  
W Bodemer ◽  
F Scheiflinger ◽  
...  
Keyword(s):  
Vaccinia Virus ◽  
Cell Lines ◽  
Expression System ◽  
Human Cell Line ◽  
Cell System ◽  
Time Saving ◽  
Expression Levels ◽  
Mammalian Cell Lines ◽  
Cell Line Hela ◽  
Virus Expression

SummaryWe have worked out an efficient and time saving procedure for the expression of recombinant human prothrombin. The glycoprotein was expressed in the vaccinia virus expression system in several mammalian cell lines. The kidney cell lines Vero and BHK and the human cell line Hela were found to efficiently secrete prothrombin. Expression levels of 3–4 µg of factor II per 106 cells per day corresponding to 18–23 mU per 106 cells per day were achieved. Since the expression levels obtained with the vaccinia virus/Vero cell system were comparable to those obtained in amplified transformed CHO cells it provides an alternative system for the efficient expression of human prothrombin and may allow to further elucidate structure-function relationships of (pro)thrombin and its various effectors.

Dynamics of fatty acid regulatory genes during ovarian development in Penaeus monodon

Reproduction ◽  
2018 ◽  
Author(s):  
Pacharawan Deenarn ◽  
Punsa Tobwor ◽  
Rungnapa Leelatanawit ◽  
Somjai Wongtriphop ◽  
Jutatip Khudet ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Ovarian Development ◽  
Penaeus Monodon ◽  
Chain Fatty Acid ◽  
Regulatory Genes ◽  
Ovarian Maturation ◽  
Long Chain Fatty Acid ◽  
Eyestalk Ablation ◽  
Regulatory Pathways ◽  
Expression Levels

The delay in ovarian maturation in farmed black tiger shrimp Penaeus monodon has resulted in the widespread practice of feeding broodstock with the polychaetes Perinereis nuntia and their unilateral eyestalk ablation. Although this practice alters fatty acid content in shrimp ovaries and hepatopancreas, its effects on fatty acid regulatory genes have yet to be systematically examined. Here, microarray analysis was performed on hepatopancreas and ovary cDNA collected from P. monodon at different ovarian maturation stages, revealing that 72 and 58 genes in fatty acid regulatory pathways were differentially expressed in hepatopancreas and ovaries respectively. Quantitative real-time PCR analysis revealed that ovarian maturation was associated with higher expression levels of acetyl-CoA acetyltransferase, acyl-CoA dehydrogenase, acyl-CoA oxidase 3 and long-chain fatty acid transport protein 4 in hepatopancreas, whereas the expression levels of 15 fatty acid regulatory genes were increased in shrimp ovaries. To distinguish the effects of different treatments, transcriptional changes were examined in P. monodon with stage 1 ovaries before polychaete feeding, after one-month of polychaete feeding and after eyestalk ablation. Polychaete feeding resulted in lower expression levels of enoyl-CoA hydratase and acyl-CoA synthetase medium-chain family member 4, while the expression level of phosphatidylinositide phosphatase SAC1 was higher in shrimp hepatopancreas and ovaries. Additionally, eyestalk ablation resulted in a higher expression level of long-chain fatty acid-CoA ligase 4 in both tissues. Together, our findings describe the dynamics of fatty acid regulatory pathways during crustacean ovarian development and provide potential target genes for alternatives to eyestalk ablation in the future.

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